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You searched for: Journal Biochimica et biophysica acta Remove constraint Journal: Biochimica et biophysica acta Publication Year 2016 Remove constraint Publication Year: 2016 Source 2016 v.1860 no.3 Remove constraint Source: 2016 v.1860 no.3
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1. A hemocyanin-derived antimicrobial peptide from the penaeid shrimp adopts an alpha-helical structure that specifically permeabilizes fungal membranes General subjects

Author:
Petit, Vanessa W.; Rolland, Jean-Luc; Blond, Alain; Cazevieille, Chantal; Djediat, Chakib; Peduzzi, Jean; Goulard, Christophe; Bachère, Evelyne; Dupont, Joëlle; Destoumieux-Garzón, Delphine; Rebuffat, Sylvie
Source:
Biochimica et biophysica acta 2016 v.1860 no.3 pp. 557-568
ISSN:
0304-4165
Subject:
Fusarium oxysporum; Litopenaeus vannamei; antimicrobial peptides; cell walls; cytoplasm; dextran; fluorescence; fungal spores; fungi; hemocyanin; homeostasis; hyphae; immunochemistry; mechanism of action; minimum inhibitory concentration; nuclear magnetic resonance spectroscopy; pathogens; permeability; plasma membrane; shrimp
Abstract:
... Hemocyanins are respiratory proteins with multiple functions. In diverse crustaceans hemocyanins can release histidine-rich antimicrobial peptides in response to microbial challenge. In penaeid shrimp, strictly antifungal peptides are released from the C-terminus of hemocyanins.The three-dimensional structure of the antifungal peptide PvHCt from Litopenaeus vannamei was determined by NMR. Its mech ...
DOI:
10.1016/j.bbagen.2015.12.010

2. A hydrogen peroxide safety valve: The reversible phosphorylation of catalase from the freeze-tolerant North American wood frog, Rana sylvatica

Author:
Dawson, Neal J.; Storey, Kenneth B.
Source:
Biochimica et biophysica acta 2016 v.1860 no.3 pp. 476-485
ISSN:
0304-4165
Subject:
AMP-activated protein kinase; Lithobates sylvaticus; freezing; ischemia; phosphoprotein phosphatase; catalase; hydrogen peroxide; superoxide dismutase; skeletal muscle; blood flow; cAMP-dependent protein kinase; frogs; oxygen; phosphorylation; cold tolerance; oxidative stress
Abstract:
... The North American wood frog, Rana sylvatica, endures whole body freezing while wintering on land and has developed multiple biochemical adaptations to elude cell/tissue damage and optimize its freeze tolerance. Blood flow is halted in the frozen state, imparting both ischemic and oxidative stress on cells. A potential build-up of H2O2 may occur due to increased superoxide dismutase activity previ ...
DOI:
10.1016/j.bbagen.2015.12.007

3. A novel fibrinolytic metalloproteinase, barnettlysin-I from Bothrops barnetti (barnett´s pitviper) snake venom with anti-platelet properties General subjects

Author:
Sanchez, Eladio Flores; Richardson, Michael; Gremski, Luiza Helena; Veiga, Silvio Sanches; Yarleque, Armando; Niland, Stephan; Lima, Augusto Martins; Estevao-Costa, Maria Inácia; Eble, Johannes Andreas
Source:
Biochimica et biophysica acta 2016 v.1860 no.3 pp. 542-556
ISSN:
0304-4165
Subject:
Bothrops; EDTA (chelating agent); blood; cation exchange; collagen; drugs; enzyme activity; fibrin; fibrinogen; fibronectins; humans; laminin; platelet aggregation; polypeptides; proteinases; snakes; venoms
Abstract:
... Viperid snake venoms contain active components that interfere with hemostasis. We report a new P-I class snake venom metalloproteinase (SVMP), barnettlysin-I (Bar-I), isolated from the venom of Bothrops barnetti and evaluated its fibrinolytic and antithrombotic potential.Bar-I was purified using a combination of molecular exclusion and cation-exchange chromatographies. We describe some biochemical ...
DOI:
10.1016/j.bbagen.2015.12.021

4. A probabilistic framework for the exploration of enzymatic capabilities based on feasible kinetics and control analysis General subjects

Author:
Saa, Pedro A.; Nielsen, Lars K.
Source:
Biochimica et biophysica acta 2016 v.1860 no.3 pp. 576-587
ISSN:
0304-4165
Subject:
enzymatic reactions; enzymes; metabolic engineering; reaction kinetics; thermodynamics; uncertainty
Abstract:
... Analysis of limiting steps within enzyme-catalyzed reactions is fundamental to understand their behavior and regulation. Methods capable of unravelling control properties and exploring kinetic capabilities of enzymatic reactions would be particularly useful for protein and metabolic engineering. While single-enzyme control analysis formalism has previously been applied to well-studied enzymatic me ...
DOI:
10.1016/j.bbagen.2015.12.015

5. CREB-binding protein contributes to the regulation of endocrine and developmental pathways in insect hemimetabolan pre-metamorphosis General subjects

Author:
Fernandez-Nicolas, Ana; Belles, Xavier
Source:
Biochimica et biophysica acta 2016 v.1860 no.3 pp. 508-515
ISSN:
0304-4165
Subject:
Blattella germanica; Drosophila melanogaster; RNA interference; ecdysis; ecdysone; embryogenesis; genomics; histones; insects; instars; metamorphosis; models; transcription (genetics)
Abstract:
... CREB-binding protein (CBP) is a promiscuous transcriptional co-regulator. In insects, CBP has been studied in the fly Drosophila melanogaster, where it is known as Nejire. Studies in D. melanogaster have revealed that Nejire is involved in the regulation of many pathways during embryo development, especially in anterior/posterior polarity, through Hedgehog and Wingless signaling, and in dorsal/ven ...
DOI:
10.1016/j.bbagen.2015.12.008

6. Fluoroquinolones stimulate the DNA cleavage activity of topoisomerase IV by promoting the binding of Mg2+ to the second metal binding site

Author:
Oppegard, Lisa M.; Schwanz, Heidi A.; Towle, Tyrell R.; Kerns, Robert J.; Hiasa, Hiroshi
Source:
Biochimica et biophysica acta 2016 v.1860 no.3 pp. 569-575
ISSN:
0304-4165
Subject:
DNA; DNA damage; DNA topoisomerase (ATP-hydrolysing); Escherichia coli; binding sites; calcium; cytotoxicity; divalent metals; drugs; fluoroquinolones; magnesium; manganese; metal ions
Abstract:
... Fluoroquinolones target bacterial type IIA topoisomerases, DNA gyrase and topoisomerase IV (Topo IV). Fluoroquinolones trap a topoisomerase–DNA covalent complex as a topoisomerase–fluoroquinolone–DNA ternary complex and ternary complex formation is critical for their cytotoxicity. A divalent metal ion is required for type IIA topoisomerase-catalyzed strand breakage and religation reactions. Recent ...
DOI:
10.1016/j.bbagen.2015.12.019
PubMed:
26723176
PubMed Central:
PMC4720527

7. Functional analysis and crystallographic structure of clotrimazole bound OleP, a cytochrome P450 epoxidase from Streptomyces antibioticus involved in oleandomycin biosynthesis General subjects

Author:
Montemiglio, Linda Celeste; Parisi, Giacomo; Scaglione, Antonella; Sciara, Giuliano; Savino, Carmelinda; Vallone, Beatrice
Source:
Biochimica et biophysica acta 2016 v.1860 no.3 pp. 465-475
ISSN:
0304-4165
Subject:
Streptomyces antibioticus; active sites; biosynthesis; chemical bonding; clotrimazole; crystal structure; cytochrome P-450; drugs; heme; ketoconazole; oleandomycin; therapeutics
Abstract:
... OleP is a cyt P450 from Streptomyces antibioticus carrying out epoxigenation of the antibiotic oleandomycin during its biosynthesis. The timing of its reaction has not been fully clarified, doubts remain regarding its substrate and catalytic mechanism.The crystal structure of OleP in complex with clotrimazole, an inhibitor of P450s used in therapy, was solved and the complex formation dynamics was ...
DOI:
10.1016/j.bbagen.2015.10.009

8. Insights into the serine protease mechanism based on structural observations of the conversion of a peptidyl serine protease inhibitor to a substrate General subjects

Author:
Jiang, Longguang; Andersen, Lisbeth Moreau; Andreasen, Peter A.; Chen, Liqing; Huang, Mingdong
Source:
Biochimica et biophysica acta 2016 v.1860 no.3 pp. 599-606
ISSN:
0304-4165
Subject:
X-radiation; active sites; catalytic activity; hydrolysis; pH; proteinase inhibitors; u-plasminogen activator
Abstract:
... Serine proteases are one of the most studied group of enzymes. Despite the extensive mechanistic studies, some crucial details remain controversial, for example, how the cleaved product is released in the catalysis reaction. A cyclic peptidyl inhibitor (CSWRGLENHRMC, upain-1) of a serine protease, urokinase-type plasminogen activator (uPA), was found to become a slow substrate and cleaved slowly u ...
DOI:
10.1016/j.bbagen.2015.12.009

9. Kiwifruit cysteine protease actinidin compromises the intestinal barrier by disrupting tight junctions General subjects

Author:
Grozdanovic, Milica M.; Čavić, Milena; Nešić, Andrijana; Andjelković, Uroš; Akbari, Peyman; Smit, Joost J.; Gavrović-Jankulović, Marija
Source:
Biochimica et biophysica acta 2016 v.1860 no.3 pp. 516-526
ISSN:
0304-4165
Subject:
allergens; animal models; blood serum; confocal microscopy; cysteine proteinases; food allergies; human cell lines; humans; intestinal mucosa; kiwifruit; mice; occludins; permeability; plant-based foods; proteolysis; tight junctions
Abstract:
... The intestinal epithelium forms a barrier that food allergens must cross in order to induce sensitization. The aim of this study was to evaluate the impact of the plant-derived food cysteine protease — actinidin (Act d1) on the integrity of intestinal epithelium tight junctions (TJs).Effects of Act d1 on the intestinal epithelium were evaluated in Caco-2 monolayers and in a mouse model by measurin ...
DOI:
10.1016/j.bbagen.2015.12.005

10. Mapping the response of human fibroblast growth factor 21 (FGF21) promoter to serum availability and lipoic acid in HepG2 hepatoma cells General subjects

Author:
Xia, Mengna; Erickson, Anjeza; Yi, Xiaohua; Moreau, Régis
Source:
Biochimica et biophysica acta 2016 v.1860 no.3 pp. 498-507
ISSN:
0304-4165
Subject:
actinomycin D; agonists; binding sites; blood serum; diet; fibroblast growth factors; glucose; hepatoma; histones; human cell lines; humans; lipids; lipoic acid; loci; messenger RNA; metabolic diseases; metabolism; nucleosomes; obesity; polypeptides; secretion; starvation; transcription (genetics); transcription factors
Abstract:
... The hormone-like polypeptide, fibroblast growth factor 21 (FGF21), is a major modulator of lipid and glucose metabolism and an exploratory treatment strategy for obesity related metabolic disorders. The costs of recombinant FGF21 and mode of delivery by injection are important constraints to its wide therapeutic use. The stimulation of endogenous FGF21 production through diet is being explored as ...
DOI:
10.1016/j.bbagen.2015.12.004

11. Multidrug resistant tumour cells shed more microvesicle-like EVs and less exosomes than their drug-sensitive counterpart cells General subjects

Author:
Lopes-Rodrigues, Vanessa; Di Luca, Alessio; Sousa, Diana; Seca, Hugo; Meleady, Paula; Henry, Michael; Lima, Raquel T.; O'Connor, Robert; Vasconcelos, M. Helena
Source:
Biochimica et biophysica acta 2016 v.1860 no.3 pp. 618-627
ISSN:
0304-4165
Subject:
P-glycoproteins; biogenesis; biomarkers; exosomes; genetic markers; models; molecular weight; multiple drug resistance; neoplasm cells; neoplasms; phenotype; prediction; protein content; proteomics
Abstract:
... Multidrug resistance (MDR) is a serious impediment to cancer treatment, with overexpression of drug efflux pumps such as P-glycoprotein (P-gp) playing a significant role. In spite of being a major clinical challenge, to date there is no simple, minimally invasive and clinically validated method for diagnosis of the MDR phenotype using non-tumour biological samples. Recently, P-gp has been found in ...
DOI:
10.1016/j.bbagen.2015.12.011

12. Probing the influence of hypermodified residues within the tRNA3Lys anticodon stem loop interacting with the A-loop primer sequence from HIV-1

Author:
Galindo-Murillo, Rodrigo; Davis, Darrell R.; Cheatham, Thomas E.
Source:
Biochimica et biophysica acta 2016 v.1860 no.3 pp. 607-617
ISSN:
0304-4165
Subject:
adenine; chemical elements; virion; Human immunodeficiency virus 1; humans; genome; quantum mechanics; nucleotides; transfer RNA; reverse transcription; binding sites; molecular dynamics; viruses
Abstract:
... Replication of the HIV-1 virus requires reverse transcription of the viral RNA genome, a process that is specifically initiated by human tRNA3ᴸʸˢ packaged within the infectious virion. The primary binding site for the tRNA involves the 3′ 18 nucleotides with an additional interaction between an adenine rich loop (A-loop) in the template and the anticodon stem–loop region of the tRNA3ᴸʸˢ. The loop ...
DOI:
10.1016/j.bbagen.2015.11.009

13. Salmonella enterica serovar Typhimurium growth is inhibited by the concomitant binding of Zn(II) and a pyrrolyl-hydroxamate to ZnuA, the soluble component of the ZnuABC transporter General subjects

Author:
Ilari, Andrea; Pescatori, Luca; Di Santo, Roberto; Battistoni, Andrea; Ammendola, Serena; Falconi, Mattia; Berlutti, Francesca; Valenti, Piera; Chiancone, Emilia
Source:
Biochimica et biophysica acta 2016 v.1860 no.3 pp. 534-541
ISSN:
0304-4165
Subject:
Gram-negative bacteria; Salmonella Typhimurium; X-radiation; active ingredients; antibiotics; binding sites; drugs; eukaryotic cells; growth retardation; human cell lines; hydroxamic acids; microbial growth; virulence; zinc
Abstract:
... Under conditions of Zn(II) deficiency, the most relevant high affinity Zn(II) transport system synthesized by many Gram-negative bacteria is the ZnuABC transporter. ZnuABC is absent in eukaryotes and plays an important role in bacterial virulence. Consequently, ZnuA, the periplasmic component of the transporter, appeared as a good target candidate to find new compounds able to contrast bacterial g ...
DOI:
10.1016/j.bbagen.2015.12.006

14. Sequential protein expression and selective labeling for in-cell NMR in human cells General subjects

Author:
Luchinat, Enrico; Secci, Erica; Cencetti, Francesca; Bruni, Paola
Source:
Biochimica et biophysica acta 2016 v.1860 no.3 pp. 527-533
ISSN:
0304-4165
Subject:
Western blotting; binding proteins; biosynthesis; gene overexpression; genes; human cell lines; humans; isotope labeling; messenger RNA; nuclear magnetic resonance spectroscopy; protein synthesis; protein-protein interactions; reverse transcriptase polymerase chain reaction; superoxide dismutase; transfection
Abstract:
... In-cell NMR is a powerful technique to investigate proteins in living human cells at atomic resolution. Ideally, when studying functional processes involving protein–protein interactions by NMR, only one partner should be isotopically labeled. Here we show that constitutive and transient protein expression can be combined with protein silencing to obtain selective protein labeling in human cells.W ...
DOI:
10.1016/j.bbagen.2015.12.023

15. Syk negatively regulates TLR4-mediated IFNβ and IL-10 production and promotes inflammatory responses in dendritic cells

Author:
Yin, Hui; Zhou, Huaxin; Kang, Yi; Zhang, Xiaoju; Duan, Xiaoxian; Alnabhan, Ridab; Liang, Shuang; Scott, David A.; Lamont, Richard J.; Shang, Jia; Wang, Huizhi
Source:
Biochimica et biophysica acta 2016 v.1860 no.3 pp. 588-598
ISSN:
0304-4165
Subject:
Toll-like receptor 4; dendritic cells; enzyme-linked immunosorbent assay; inflammation; interleukin-10; interleukin-6; loss-of-function mutation; mice; neutralization; phosphatidylinositol 3-kinase; phosphorylation; tau-protein kinase; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... While Syk has been shown to associate with TLR4, the immune consequences of Syk–TLR interactions and related molecular mechanisms are unclear.Gain- and loss-of-function approaches were utilized to determine the regulatory function of Syk and elucidate the related molecular mechanisms in TLR4-mediated inflammatory responses. Cytokine production was measured by ELISA and phosphorylation of signaling ...
DOI:
10.1016/j.bbagen.2015.12.012
PubMed:
26708990
PubMed Central:
PMC5321715

16. Towards a characterization of the structural determinants of specificity in the macrocyclizing thioesterase for deoxyerythronolide B biosynthesis General subjects

Author:
Argyropoulos, Panos; Bergeret, Fabien; Pardin, Christophe; Reimer, Janice M.; Pinto, Atahualpa; Boddy, Christopher N.; Schmeing, T. Martin
Source:
Biochimica et biophysica acta 2016 v.1860 no.3 pp. 486-497
ISSN:
0304-4165
Subject:
biosynthesis; biphenyl; erythromycin; hydrolysis; polyketide synthases; therapeutics; transesterification
Abstract:
... Type I polyketide synthases (PKSs) are giant multidomain proteins that synthesize many therapeutics and other natural products. The synthesis proceeds by a thiotemplate mechanism whereby intermediates are covalently attached to the PKS. The release of the final polyketide is catalyzed by the terminal thioesterase (TE) domain through hydrolysis, transesterification, or macrocyclization. The PKS 6-d ...
DOI:
10.1016/j.bbagen.2015.11.007