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1. Active site mapping of Loxosceles phospholipases D: Biochemical and biological features Molecular and cell biology of lipids

Author:
Vuitika, L.; Chaves-Moreira, D.; Caruso, I.; Lima, M.A.; Matsubara, F.H.; Murakami, M.T.; Takahashi, H.K.; Toledo, M.S.; Coronado, M.A.; Nader, H.B.; Senff-Ribeiro, A.; Chaim, O.M.; Arni, R.K.; Veiga, S.S.
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 970-979
ISSN:
1388-1981
Subject:
Loxosceles; active sites; antibodies; bioactive properties; blood serum; circular dichroism spectroscopy; differential scanning calorimetry; hemolysis; inflammation; mutants; permeability; phospholipase D; phospholipases; phospholipids; renal failure; toxins; venoms
Abstract:
... Brown spider phospholipases D from Loxosceles venoms are among the most widely studied toxins since they induce dermonecrosis, triggering inflammatory responses, increase vascular permeability, cause hemolysis, and renal failure. The catalytic (H12 and H47) and metal-ion binding (E32 and D34) residues in Loxosceles intermedia phospholipase D (LiRecDT1) were mutated to understand their roles in the ...
DOI:
10.1016/j.bbalip.2016.05.009

2. Assessing compartmentalized flux in lipid metabolism with isotopes

Author:
Allen, Doug K.
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1226-1242
ISSN:
1388-1981
Subject:
acetyl coenzyme A; biochemical pathways; biosynthesis; cytosol; experimental design; fatty acids; instrumentation; lipid metabolism; metabolites; organelles; radiolabeling; radionuclides; spatial variation; stable isotopes; tissue distribution; tissues
Abstract:
... Metabolism in plants takes place across multiple cell types and within distinct organelles. The distributions equate to spatial heterogeneity; though the limited means to experimentally assess metabolism frequently involve homogenizing tissues and mixing metabolites from different locations. Most current isotope investigations of metabolism therefore lack the ability to resolve spatially distinct ...
Handle:
10113/62904
DOI:
10.1016/j.bbalip.2016.03.017
Chorus Open Access:
10.1016/j.bbalip.2016.03.017

3. Biochemical characterization of the tomato phosphatidylinositol-specific phospholipase C (PI-PLC) family and its role in plant immunity Molecular and cell biology of lipids

Author:
Abd-El-Haliem, Ahmed M.; Vossen, Jack H.; van Zeijl, Arjan; Dezhsetan, Sara; Testerink, Christa; Seidl, Michael F.; Beck, Martina; Strutt, James; Robatzek, Silke; Joosten, Matthieu H.A.J.
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1365-1378
ISSN:
1388-1981
Subject:
Arabidopsis thaliana; Solanum lycopersicum; abiotic stress; calcium; diacylglycerols; flagellin; genome; immunity; ions; nucleotide sequences; pH; phospholipase C; recombinant proteins; substrate specificity; tomatoes
Abstract:
... Plants possess effective mechanisms to quickly respond to biotic and abiotic stresses. The rapid activation of phosphatidylinositol-specific phospholipase C (PLC) enzymes occurs early after the stimulation of plant immune-receptors. Genomes of different plant species encode multiple PLC homologs belonging to one class, PLCζ. Here we determined whether all tomato homologs encode active enzymes and ...
DOI:
10.1016/j.bbalip.2016.01.017

4. Broad substrate specificity of phosphotransbutyrylase from Listeria monocytogenes: A potential participant in an alternative pathway for provision of acyl CoA precursors for fatty acid biosynthesis

Author:
Sirobhushanam, Sirisha; Galva, Charitha; Sen, Suranjana; Wilkinson, Brian J.; Gatto, Craig
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1102-1110
ISSN:
1388-1981
Subject:
Listeria monocytogenes; acetyl coenzyme A; acyl coenzyme A; additives; amino acids; biosynthesis; branched chain fatty acids; butyrates; catalytic activity; chemical structure; enzymes; foodborne illness; listeriosis; mutants; operon; substrate specificity
Abstract:
... Listeria monocytogenes, the causative organism of the serious food-borne disease listeriosis, has a membrane abundant in branched-chain fatty acids (BCFAs). BCFAs are normally biosynthesized from branched-chain amino acids via the activity of branched chain α-keto acid dehydrogenase (Bkd), and disruption of this pathway results in reduced BCFA content in the membrane. Short branched-chain carboxyl ...
DOI:
10.1016/j.bbalip.2016.06.003
PubMed:
27320015
PubMed Central:
PMC4947441

5. Construction of a cyanobacterium synthesizing cyclopropane fatty acids Molecular and cell biology of lipids

Author:
Machida, Shuntaro; Shiraiwa, Yoshihiro; Suzuki, Iwane
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 980-987
ISSN:
1388-1981
Subject:
Escherichia coli; Synechocystis sp. PCC 6714; Synechocystis sp. PCC 6803; biofuels; fatty acids; genes; microalgae; oxidation; photosynthesis; temperature
Abstract:
... Microalgae have received much attention as a next-generation source of biomass energy. However, most of the fatty acids (FAs) from microalgae are multiply unsaturated; thus, the biofuels derived from them are fluid, but vulnerable to oxidation. In this study, we attempted to synthesize cyclopropane FAs in the cyanobacterium Synechocystis sp. PCC 6803 by expressing the cfa gene for cyclopropane FA ...
DOI:
10.1016/j.bbalip.2016.05.012

6. Critical role for cytosolic group IVA phospholipase A2 in early adipocyte differentiation and obesity Molecular and cell biology of lipids

Author:
Peña, Lucía; Meana, Clara; Astudillo, Alma M.; Lordén, Gema; Valdearcos, Martín; Sato, Hiroyasu; Murakami, Makoto; Balsinde, Jesús; Balboa, María A.
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1083-1095
ISSN:
1388-1981
Subject:
adipocytes; adipogenesis; adipose tissue; animals; arachidonic acid; cell cycle; comorbidity; high fat diet; obesity; phospholipase A2; stem cells; transcription factors
Abstract:
... Adipogenesis is the process of differentiation of immature mesenchymal stem cells into adipocytes. Elucidation of the mechanisms that regulate adipocyte differentiation is key for the development of novel therapies for the control of obesity and related comorbidities. Cytosolic group IVA phospholipase A2 (cPLA2α) is the pivotal enzyme in receptor-mediated arachidonic acid (AA) mobilization and att ...
DOI:
10.1016/j.bbalip.2016.06.004

7. Digalactosyldiacylglycerol is essential in Synechococcus elongatus PCC 7942, but its function does not depend on its biosynthetic pathway Molecular and cell biology of lipids

Author:
Maida, Eri; Awai, Koichiro
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1309-1314
ISSN:
1388-1981
Subject:
Chloroflexus aurantiacus; Synechococcus sp. PCC 7942; autotrophs; biochemical pathways; galactose; genes; lipid composition; lipids; photosynthesis; plants (botany); thylakoids
Abstract:
... Digalactosyldiacylglycerol (DGDG) is a major component of thylakoid membranes, occupying approximately 20% of the membrane system. This lipid composition is conserved from cyanobacteria to the chloroplasts of terrestrial plants, suggesting that DGDG is important for the function of photosynthetic membranes. Here we isolated the gene for DGDG synthase in the cyanobacterium Synechococcus elongatus P ...
DOI:
10.1016/j.bbalip.2016.03.011

8. Effect of preduodenal lipase inhibition in suckling rats on dietary octanoic acid (C8:0) gastric absorption and plasma octanoylated ghrelin concentration Molecular and cell biology of lipids

Author:
Lemarié, F.; Cavalier, J.-F.; Garcia, C.; Boissel, F.; Point, V.; Catheline, D.; Legrand, P.; Carrière, F.; Rioux, V.
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1111-1120
ISSN:
1388-1981
Subject:
absorption; carboxylic ester hydrolases; digestion; emulsions; gastric mucosa; ghrelin; ghrelin receptors; ligands; lipolysis; octanoic acid; rats; suckling; triolein
Abstract:
... Part of medium chain fatty acids (MCFAs) coming from dietary triglycerides (TGs) can be directly absorbed through the gastric mucosa after the action of preduodenal lipase (lingual lipase in the rat). MCFA gastric absorption, particularly that of octanoic acid (C8:0), may have a physiological importance in the octanoylation of ghrelin, the orexigenic gastric peptide acting as an endogenous ligand ...
DOI:
10.1016/j.bbalip.2016.06.009

9. Hepatitis C virus p7 mediates membrane-to-membrane adhesion Molecular and cell biology of lipids

Author:
Lee, Gi Young; Lee, Sora; Lee, Hye-Ra; Yoo, Young Do
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1096-1101
ISSN:
1388-1981
Subject:
Hepatitis C virus; adhesion; ion channels; lipids; pH
Abstract:
... Viroporin p7 of the hepatitis C virus (HCV) acts as an ion channel for pH equilibration to stabilize HCV particles; most studies of p7 have focused on this role. However, pH equilibration by p7 via its ion channel activity does not fully explain the importance of p7 in HCV particle production. Indeed, several researchers have suggested p7 to have an unidentified ion channel-independent function. H ...
DOI:
10.1016/j.bbalip.2016.06.011

10. Hormone-sensitive lipase deficiency disturbs lipid composition of plasma membrane microdomains from mouse testis Molecular and cell biology of lipids

Author:
Casado, María E.; Pastor, Oscar; García-Seisdedos, David; Huerta, Lydia; Kraemer, Fredric B.; Lasunción, Miguel A.; Martín-Hidalgo, Antonia; Busto, Rebeca
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1142-1150
ISSN:
1388-1981
Subject:
acylglycerols; carboxylic ester hydrolases; cholesterol; fractionation; lipid composition; male fertility; males; mice; monounsaturated fatty acids; phosphatidylcholines; phosphatidylethanolamines; phosphatidylserines; plasma membrane; proteins; signal transduction; sphingolipids; testes
Abstract:
... Hormone-sensitive lipase (HSL) is an intracellular neutral lipase capable of hydrolyzing acylglycerols, as well as cholesteryl and retinyl esters. In mice, HSL deficiency results in male sterility. Lipid rafts, a plasma membrane microdomain enriched in cholesterol, sphingolipids and saturated glycerophospholipids, comprise a highly dynamic clustering of proteins and lipids that play a central role ...
DOI:
10.1016/j.bbalip.2016.06.018

11. How did nature engineer the highest surface lipid accumulation among plants? Exceptional expression of acyl-lipid-associated genes for the assembly of extracellular triacylglycerol by Bayberry (Myrica pensylvanica) fruits Molecular and cell biology of lipids

Author:
Simpson, Jeffrey P.; Thrower, Nicholas; Ohlrogge, John B.
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1243-1252
ISSN:
1388-1981
Subject:
Morella caroliniensis; amino acid sequences; biosynthesis; cherries; cutin; diacylglycerols; esterification; evolution; gene expression; gene expression regulation; genes; leaves; lipid metabolism; mesocarp; microscopy; saturated fatty acids; secretion; seeds; tissues; tomatoes; triacylglycerols
Abstract:
... Bayberry (Myrica pensylvanica) fruits are covered with a remarkably thick layer of crystalline wax consisting of triacylglycerol (TAG) and diacylglycerol (DAG) esterified exclusively with saturated fatty acids. As the only plant known to accumulate soluble glycerolipids as a major component of surface waxes, Bayberry represents a novel system to investigate neutral lipid biosynthesis and lipid sec ...
DOI:
10.1016/j.bbalip.2016.01.022

12. Inflammatory intracellular pathways activated by electronegative LDL in monocytes Molecular and cell biology of lipids

Author:
Estruch, Montserrat; Sanchez-Quesada, Jose Luis; Ordoñez-Llanos, Jordi; Benitez, Sonia
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 963-969
ISSN:
1388-1981
Subject:
Toll-like receptor 4; cyclic AMP; enzyme-linked immunosorbent assay; interleukin-10; interleukin-6; low density lipoprotein; mitogen-activated protein kinase; monocytes; phosphatidylinositol 3-kinase; phosphorylation; transcription factor NF-kappa B
Abstract:
... Electronegative LDL (LDL(−)) is a plasma LDL subfraction that induces cytokine release in monocytes through toll-like receptor 4 (TLR4) activation. However, the intracellular pathways induced by LDL(−) downstream TLR4 activation are unknown. We aimed to identify the pathways activated by LDL(−) leading to cytokine release in monocytes.We determined LDL(−)-induced activation of several intracellula ...
DOI:
10.1016/j.bbalip.2016.05.010

13. Integrin-mediated cell migration is blocked by inhibitors of human neuraminidase Molecular and cell biology of lipids

Author:
Jia, Feng; Howlader, Md. Amran; Cairo, Christopher W.
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1170-1179
ISSN:
1388-1981
Subject:
adhesion; cell movement; cytoskeleton; enzyme activity; gangliosides; humans; integrins; isozymes; phosphorylation; receptors; sialidase
Abstract:
... Integrins are critical receptors in cell migration and adhesion. A number of mechanisms are known to regulate the function of integrins, including phosphorylation, conformational change, and cytoskeletal anchoring. We investigated whether native neuraminidase (Neu, or sialidase) enzymes which modify glycolipids could play a role in regulating integrin-mediated cell migration. Using a scratch assay ...
DOI:
10.1016/j.bbalip.2016.06.013

14. Isolation and characterization of a mutant defective in triacylglycerol accumulation in nitrogen-starved Chlamydomonas reinhardtii Molecular and cell biology of lipids

Author:
Hung, Chun-Hsien; Kanehara, Kazue; Nakamura, Yuki
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1282-1293
ISSN:
1388-1981
Subject:
Chlamydomonas reinhardtii; acetates; acetyl coenzyme A; beta-galactosidase; biodiesel; biosynthesis; culture media; fatty acid composition; fructose 6-phosphate; fuel production; genes; glucose 6-phosphate; glycolysis; hydrolysis; metabolites; metabolomics; mutants; nitrogen; photosynthesis; starch granules; starvation; triacylglycerols; tricarboxylic acid cycle
Abstract:
... Triacylglycerol (TAG), a major source of biodiesel production, accumulates in nitrogen-starved Chlamydomonas reinhardtii. However, the metabolic pathway of starch-to-TAG conversion remains elusive because an enzyme that affects the starch degradation is unknown. Here, we isolated a new class of mutant bgal1, which expressed an overaccumulation of starch granules and defective photosynthetic growth ...
DOI:
10.1016/j.bbalip.2016.04.001

15. Knockdown of triglyceride synthesis does not enhance palmitate lipotoxicity or prevent oleate-mediated rescue in rat hepatocytes

Author:
Leamy, Alexandra K.; Hasenour, Clinton M.; Egnatchik, Robert A.; Trenary, Irina A.; Yao, Cong-Hui; Patti, Gary J.; Shiota, Masakazu; Young, Jamey D.
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1005-1014
ISSN:
1388-1981
Subject:
diacylglycerol acyltransferase; esterification; hepatocytes; lipotoxicity; oleic acid; palmitates; phospholipids; protective effect; rats; saturated fatty acids; toxicity; triacylglycerols
Abstract:
... Experiments in a variety of cell types, including hepatocytes, consistently demonstrate the acutely lipotoxic effects of saturated fatty acids, such as palmitate (PA), but not unsaturated fatty acids, such as oleate (OA). PA+OA co-treatment fully prevents PA lipotoxicity through mechanisms that are not well defined but which have been previously attributed to more efficient esterification and sequ ...
DOI:
10.1016/j.bbalip.2016.05.013
PubMed:
27249207
PubMed Central:
PMC4947436

16. Lipids in plant–microbe interactions Molecular and cell biology of lipids

Author:
Siebers, Meike; Brands, Mathias; Wewer, Vera; Duan, Yanjiao; Hölzl, Georg; Dörmann, Peter
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1379-1395
ISSN:
1388-1981
Subject:
bacteria; ceramides; diacylglycerols; free fatty acids; fungi; jasmonic acid; oxylipins; phospholipids; systemic acquired resistance
Abstract:
... Bacteria and fungi can undergo symbiotic or pathogenic interactions with plants. Membrane lipids and lipid-derived molecules from the plant or the microbial organism play important roles during the infection process. For example, lipids (phospholipids, glycolipids, sphingolipids, sterol lipids) are involved in establishing the membrane interface between the two organisms. Furthermore, lipid-derive ...
DOI:
10.1016/j.bbalip.2016.02.021

17. Lipids in pollen — They are different Molecular and cell biology of lipids

Author:
Ischebeck, Till
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1315-1328
ISSN:
1388-1981
Subject:
Arabidopsis; droplets; evolution; fatty acids; galactolipids; gametophytes; lipid composition; males; mutants; ovules; phenotype; pollen; pollen tubes; proteins; spermatozoa; sphingolipids; stigma; triacylglycerols
Abstract:
... During evolution, the male gametophyte of Angiosperms has been severely reduced to the pollen grain, consisting of a vegetative cell containing two sperm cells.This vegetative cell has to deliver the sperm cells from the stigma through the style to the ovule. It does so by producing a pollen tube and elongating it to many centimeters in length in some species, requiring vast amounts of fatty acid ...
DOI:
10.1016/j.bbalip.2016.03.023

18. Low-density lipoprotein upregulate SR-BI through Sp1 Ser702 phosphorylation in hepatic cells Molecular and cell biology of lipids

Author:
Yang, Fan; Du, Yu; Zhang, Jin; Jiang, Zhibo; Wang, Li; Hong, Bin
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1066-1075
ISSN:
1388-1981
Subject:
acetylation; blood lipids; cardiovascular diseases; genes; hepatocytes; high density lipoprotein cholesterol; histone acetyltransferase; histone deacetylase; histones; lipid content; low density lipoprotein; mass spectrometry; phosphorylation; precipitin tests; promoter regions; risk; site-directed mutagenesis; transcription factors
Abstract:
... Scavenger receptor class B type I (SR-BI) is one of the key proteins in the process of reverse cholesterol transport (RCT), and its major function is to uptake high density lipoprotein (HDL) cholesterol from plasma into liver cells. The regulation of SR-BI expression is important for controlling serum lipid content and reducing the risks of cardiovascular diseases. Here we found that SR-BI express ...
DOI:
10.1016/j.bbalip.2016.06.001

19. Mapping a kingdom-specific functional domain of squalene synthase Molecular and cell biology of lipids

Author:
Linscott, Kristin B.; Niehaus, Thomas D.; Zhuang, Xun; Bell, Stephen A.; Chappell, Joe
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1049-1057
ISSN:
1388-1981
Subject:
Saccharomyces cerevisiae; active sites; animals; biosynthesis; chimerism; enzymes; ergosterol; eukaryotic cells; genes; growth retardation; knockout mutants; mutation; phenotype; plants (botany); squalene; toxicity; yeasts
Abstract:
... Squalene synthase catalyzes the first committed step in sterol biosynthesis and consists of both an amino-terminal catalytic domain and a carboxy-terminal domain tethering the enzyme to the ER membrane. While the overall architecture of this enzyme is identical in eukaryotes, it was previously shown that plant and animal genes cannot complement a squalene synthase knockout mutation in yeast unless ...
DOI:
10.1016/j.bbalip.2016.06.008

20. Mechanisms and metabolic regulation of PPARα activation in Nile tilapia (Oreochromis niloticus) Molecular and cell biology of lipids

Author:
Ning, Li-Jun; He, An-Yuan; Li, Jia-Min; Lu, Dong-Liang; Jiao, Jian-Gang; Li, Ling-Yu; Li, Dong-Liang; Zhang, Mei-Ling; Chen, Li-Qiao; Du, Zhen-Yu
Source:
Biochimica et biophysica acta 2016 v.1861 no.9 pp. 1036-1048
ISSN:
1388-1981
Subject:
DNA; Oreochromis niloticus; agonists; beta oxidation; blood glucose; brain; carbohydrate metabolism; carnitine; cytochrome b; dephosphorylation; energy; fasting; fatty acids; fenofibrate; fish; gene expression; heart; hepatocytes; homeostasis; insulin; lactic acid; lipemic effect; liver; mammals; messenger RNA; mitochondria; nutrients; palmitates; peroxisome proliferator-activated receptors; phosphorylation; protein synthesis; tissues; transcriptomics; triacylglycerols
Abstract:
... Although the key metabolic regulatory functions of mammalian peroxisome proliferator-activated receptor α (PPARα) have been thoroughly studied, the molecular mechanisms and metabolic regulation of PPARα activation in fish are less known. In the first part of the present study, Nile tilapia (Nt)PPARα was cloned and identified, and high mRNA expression levels were detected in the brain, liver, and h ...
DOI:
10.1016/j.bbalip.2016.06.005