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You searched for: Journal Biochimica et biophysica acta Remove constraint Journal: Biochimica et biophysica acta Publication Year 2013 Remove constraint Publication Year: 2013 Source 2013 v.1827 no.6 Remove constraint Source: 2013 v.1827 no.6
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1. An NMR comparison of the light-harvesting complex II (LHCII) in active and photoprotective states reveals subtle changes in the chlorophyll a ground-state electronic structures

Author:
Pandit, Anjali; Reus, Michael; Morosinotto, Tomas; Bassi, Roberto; Holzwarth, Alfred R.; de Groot, Huub J.M.
Source:
Biochimica et biophysica acta 2013 v.1827 no.6 pp. 738-744
ISSN:
0005-2728
Subject:
Chlamydomonas reinhardtii; X-radiation; carbon; chlorophyll; energy; light harvesting complex; lutein; neoxanthin; nuclear magnetic resonance spectroscopy; photostability; photosystem II; solar radiation
Abstract:
... To protect the photosynthetic apparatus against photo-damage in high sunlight, the photosynthetic antenna of oxygenic organisms can switch from a light-harvesting to a photoprotective mode through the process of non-photochemical quenching (NPQ). There is growing evidence that light-harvesting proteins of photosystem II participate in photoprotection by a built-in capacity to switch their conforma ...
DOI:
10.1016/j.bbabio.2013.02.015

2. Atomistic simulations indicate cardiolipin to have an integral role in the structure of the cytochrome bc1 complex

Author:
Pöyry, Sanja; Cramariuc, Oana; Postila, Pekka A.; Kaszuba, Karol; Sarewicz, Marcin; Osyczka, Artur; Vattulainen, Ilpo; Róg, Tomasz
Source:
Biochimica et biophysica acta 2013 v.1827 no.6 pp. 769-778
ISSN:
0005-2728
Subject:
Rhodobacter capsulatus; bacteria; electron transfer; heme; lipid bilayers; lipids; mitochondrial membrane; molecular dynamics
Abstract:
... The reaction mechanism of the cytochrome (cyt) bc1 complex relies on proton and electron transfer to/from the substrate quinone/quinol, which in turn generate a proton gradient across the mitochondrial membrane. Cardiolipin (CL) have been suggested to play an important role in cyt bc1 function by both ensuring the structural integrity of the protein complex and also by taking part in the proton up ...
DOI:
10.1016/j.bbabio.2013.03.005

3. A variant conferring cofactor-dependent assembly of Escherichia coli dimethylsulfoxide reductase

Author:
Tang, Huipo; Rothery, Richard A.; Weiner, Joel H.
Source:
Biochimica et biophysica acta 2013 v.1827 no.6 pp. 730-737
ISSN:
0005-2728
Subject:
Escherichia coli; amino acid substitution; antagonists; apoproteins; cell membranes; dimethyl sulfoxide; dimethylsulfoxide reductase; guanine; molybdates; molybdenum; protein subunits
Abstract:
... We have investigated the final steps of complex iron–sulfur molybdoenzyme (CISM) maturation using Escherichia coli DMSO reductase (DmsABC) as a model system. The catalytic subunit of this enzyme, DmsA, contains an iron–sulfur cluster (FS0) and a molybdo-bis(pyranopterin guanine dinucleotide) cofactor (Mo-bisPGD). We have identified a variant of DmsA (Cys59Ser) that renders enzyme maturation sensit ...
DOI:
10.1016/j.bbabio.2013.02.009

4. Catalytically-relevant electron transfer between two hemes bL in the hybrid cytochrome bc1-like complex containing a fusion of Rhodobacter sphaeroides and capsulatus cytochromes b

Author:
Czapla, Monika; Cieluch, Ewelina; Borek, Arkadiusz; Sarewicz, Marcin; Osyczka, Artur
Source:
Biochimica et biophysica acta 2013 v.1827 no.6 pp. 751-760
ISSN:
0005-2728
Subject:
Rhodobacter capsulatus; Rhodobacter sphaeroides; bacteria; cytochrome b; electron transfer; engineering
Abstract:
... To address mechanistic questions about the functioning of dimeric cytochrome bc1 new genetic approaches have recently been developed. They were specifically designed to enable construction of asymmetrically-mutated variants suitable for functional studies. One approach exploited a fusion of two cytochromes b that replaced the separate subunits in the dimer. The fusion protein, built from two copie ...
DOI:
10.1016/j.bbabio.2013.02.007
PubMed:
23428397
PubMed Central:
PMC4330944

5. Characterization of singlet oxygen production and its involvement in photodamage of Photosystem II in the cyanobacterium Synechocystis PCC 6803 by histidine-mediated chemical trapping

Author:
Rehman, Ateeq Ur; Cser, Krisztián; Sass, László; Vass, Imre
Source:
Biochimica et biophysica acta 2013 v.1827 no.6 pp. 689-698
ISSN:
0005-2728
Subject:
Synechocystis sp. PCC 6803; chlorophyll; deuterium oxide; electrodes; fluorescence; histidine; light intensity; lighting; models; mutants; oxygen; oxygen production; photoinhibition; photosystem II; singlet oxygen; trapping
Abstract:
... Singlet oxygen production in intact cells of the cynobacterium Synechocystis 6803 was studied using chemical trapping by histidine, which leads to O2 uptake during illumination. The rate of O2 uptake, measured by a standard Clark-type electrode, is enhanced in the presence of D2O, which increases the lifetime of 1O2, and suppressed by the 1O2 quencher NaN3. Due to the limited mobility of 1O2 these ...
DOI:
10.1016/j.bbabio.2013.02.016

6. Conversion of Corynebacterium glutamicum from an aerobic respiring to an aerobic fermenting bacterium by inactivation of the respiratory chain

Author:
Koch-Koerfges, Abigail; Pfelzer, Nina; Platzen, Laura; Oldiges, Marco; Bott, Michael
Source:
Biochimica et biophysica acta 2013 v.1827 no.6 pp. 699-708
ISSN:
0005-2728
Subject:
Corynebacterium glutamicum; acetates; adenosine triphosphate; aerobiosis; bacteria; biomass; electron transport chain; fumarate reductase (quinol); glucose; hydrolysis; mutants; oxygen; oxygen consumption; peptones; proton-motive force; protons; succinic acid
Abstract:
... In this study a comparative analysis of three Corynebacterium glutamicum ATCC 13032 respiratory chain mutants lacking either the cytochrome bd branch (ΔcydAB), or the cytochrome bc1–aa3 branch (Δqcr), or both branches was performed. The lack of cytochrome bd oxidase was inhibitory only under conditions of oxygen limitation, whereas the absence of a functional cytochrome bc1–aa3 supercomplex led to ...
DOI:
10.1016/j.bbabio.2013.02.004

7. Deletion of β-strands 9 and 10 converts VDAC1 voltage-dependence in an asymmetrical process

Author:
Reina, Simona; Magrì, Andrea; Lolicato, Marco; Guarino, Francesca; Impellizzeri, Agata; Maier, Elke; Benz, Roland; Ceccarelli, Matteo; De Pinto, Vito; Messina, Angela
Source:
Biochimica et biophysica acta 2013 v.1827 no.6 pp. 793-805
ISSN:
0005-2728
Subject:
apoptosis; chimerism; metabolism; mitochondrial membrane; molecular dynamics; mutants; permeability
Abstract:
... Voltage-dependent anion selective channel isoform1 maintains the permeability of the outer mitochondrial membrane. Its voltage-gating properties are relevant in bioenergetic metabolism and apoptosis. The N-terminal domain is suspected to be involved in voltage-gating, due to its peculiar localization. However this issue is still controversial. In this work we exchanged or deleted the β-strands tha ...
DOI:
10.1016/j.bbabio.2013.03.007

8. Key role of water in proton transfer at the Qo-site of the cytochrome bc1 complex predicted by atomistic molecular dynamics simulations

Author:
Postila, Pekka A.; Kaszuba, Karol; Sarewicz, Marcin; Osyczka, Artur; Vattulainen, Ilpo; Róg, Tomasz
Source:
Biochimica et biophysica acta 2013 v.1827 no.6 pp. 761-768
ISSN:
0005-2728
Subject:
Rhodobacter capsulatus; electron transport chain; lipid bilayers; molecular dynamics; protein subunits; protons; sulfur
Abstract:
... Cytochrome (cyt) bc1 complex, which is an integral part of the respiratory chain and related energy-conserving systems, has two quinone-binding cavities (Qo- and Qi-sites), where the substrate participates in electron and proton transfer. Due to its complexity, many of the mechanistic details of the cyt bc1 function have remained unclear especially regarding the substrate binding at the Qo-site. I ...
DOI:
10.1016/j.bbabio.2013.02.005

9. Light harvesting complexes of Chromera velia, photosynthetic relative of apicomplexan parasites

Author:
Tichy, Josef; Gardian, Zdenko; Bina, David; Konik, Peter; Litvin, Radek; Herbstova, Miroslava; Pain, Arnab; Vacha, Frantisek
Source:
Biochimica et biophysica acta 2013 v.1827 no.6 pp. 723-729
ISSN:
0005-2728
Subject:
circular dichroism spectroscopy; solar radiation; electron microscopy; Rhodophyta; Miozoa; chlorophyll binding proteins; photosystem I; molecular weight; autotrophs; antennae; Bacillariophyceae
Abstract:
... The structure and composition of the light harvesting complexes from the unicellular alga Chromera velia were studied by means of optical spectroscopy, biochemical and electron microscopy methods. Two different types of antennae systems were identified. One exhibited a molecular weight (18–19kDa) similar to FCP (fucoxanthin chlorophyll protein) complexes from diatoms, however, single particle anal ...
DOI:
10.1016/j.bbabio.2013.02.002

10. Monogalactosyldiacylglycerol deficiency in tobacco inhibits the cytochrome b6f-mediated intersystem electron transport process and affects the photostability of the photosystem II apparatus

Author:
Wu, Wang; Ping, Wenli; Wu, Hanying; Li, Minchun; Gu, Dan; Xu, Yinong
Source:
Biochimica et biophysica acta 2013 v.1827 no.6 pp. 709-722
ISSN:
0005-2728
Subject:
chlorophyll; chronic exposure; electron transfer; fluorescence; growth retardation; immunoblotting; phenotype; photostability; photosystem II; polyacrylamide gel electrophoresis; thylakoids; tobacco; transgenic plants
Abstract:
... Monogalactosyldiacylglycerol (MGDG) is the most abundant lipid component of the thylakoid membrane. Although MGDG is believed to be important in sustaining the structure and function of the photosynthetic membrane, its exact role in photosynthesis in vivo requires further investigation. In this study, the transgenic tobacco plant M18, which has an MGDG deficiency of approximately 53%, and which co ...
DOI:
10.1016/j.bbabio.2013.02.013

11. On the analysis of non-photochemical chlorophyll fluorescence quenching curves

Author:
Holzwarth, Alfred R.; Lenk, Dagmar; Jahns, Peter
Source:
Biochimica et biophysica acta 2013 v.1827 no.6 pp. 786-792
ISSN:
0005-2728
Subject:
autotrophs; chlorophyll; fluorescence; lighting
Abstract:
... Non-photochemical quenching (NPQ) protects photosynthetic organisms against photodamage by high light. One of the key measuring parameters for characterizing NPQ is the high-light induced decrease in chlorophyll fluorescence. The originally measured data are maximal fluorescence (Fm′) signals as a function of actinic illumination time (Fm′(t)). Usually these original data are converted into the so ...
DOI:
10.1016/j.bbabio.2013.02.011

12. Photochemical trapping heterogeneity as a function of wavelength, in plant photosystem I (PSI–LHCI)

Author:
Jennings, Robert C.; Zucchelli, Giuseppe; Santabarbara, Stefano
Source:
Biochimica et biophysica acta 2013 v.1827 no.6 pp. 779-785
ISSN:
0005-2728
Subject:
absorption; corn; energy transfer; fluorescence; photosystem I; trapping; wavelengths
Abstract:
... In the present paper the marked changes in photochemical trapping time over the absorption/fluorescence band of isolated PSI–LHCI are studied by means of time resolved fluorescence decay measurements. For emission at 680–690nm the effective trapping time is close to 17–18ps, and represents the effective trapping time from the bulk antenna. At wavelengths above 700nm the effective trapping time inc ...
DOI:
10.1016/j.bbabio.2013.03.008

13. Relative importance of driving force and electrostatic interactions in the reduction of multihaem cytochromes by small molecules

Author:
Quintas, Pedro O.; Cepeda, Andreia P.; Borges, Nuno; Catarino, Teresa; Turner, David L.
Source:
Biochimica et biophysica acta 2013 v.1827 no.6 pp. 745-750
ISSN:
0005-2728
Subject:
Methylophilus methylotrophus; bioremediation; cytochromes; electron transfer; electrostatic interactions; energy; ionic strength; mutants; pH; redox potential; sodium; thermodynamics
Abstract:
... Multihaem cytochromes are essential to the energetics of organisms capable of bioremediation and energy production. The haems in several of these cytochromes have been discriminated thermodynamically and their individual rates of reduction by small electron donors were characterized. The kinetic characterization of individual haems used the Marcus theory of electron transfer and assumed that the r ...
DOI:
10.1016/j.bbabio.2013.02.006