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- Author:
- Pandit, Anjali; Reus, Michael; Morosinotto, Tomas; Bassi, Roberto; Holzwarth, Alfred R.; de Groot, Huub J.M.
- Source:
- Biochimica et biophysica acta 2013 v.1827 no.6 pp. 738-744
- ISSN:
- 0005-2728
- Subject:
- Chlamydomonas reinhardtii; X-radiation; carbon; chlorophyll; energy; light harvesting complex; lutein; neoxanthin; nuclear magnetic resonance spectroscopy; photostability; photosystem II; solar radiation
- Abstract:
- ... To protect the photosynthetic apparatus against photo-damage in high sunlight, the photosynthetic antenna of oxygenic organisms can switch from a light-harvesting to a photoprotective mode through the process of non-photochemical quenching (NPQ). There is growing evidence that light-harvesting proteins of photosystem II participate in photoprotection by a built-in capacity to switch their conforma ...
- DOI:
- 10.1016/j.bbabio.2013.02.015
-
https://dx.doi.org/10.1016/j.bbabio.2013.02.015
- Author:
- Pöyry, Sanja; Cramariuc, Oana; Postila, Pekka A.; Kaszuba, Karol; Sarewicz, Marcin; Osyczka, Artur; Vattulainen, Ilpo; Róg, Tomasz
- Source:
- Biochimica et biophysica acta 2013 v.1827 no.6 pp. 769-778
- ISSN:
- 0005-2728
- Subject:
- Rhodobacter capsulatus; bacteria; electron transfer; heme; lipid bilayers; lipids; mitochondrial membrane; molecular dynamics
- Abstract:
- ... The reaction mechanism of the cytochrome (cyt) bc1 complex relies on proton and electron transfer to/from the substrate quinone/quinol, which in turn generate a proton gradient across the mitochondrial membrane. Cardiolipin (CL) have been suggested to play an important role in cyt bc1 function by both ensuring the structural integrity of the protein complex and also by taking part in the proton up ...
- DOI:
- 10.1016/j.bbabio.2013.03.005
-
https://dx.doi.org/10.1016/j.bbabio.2013.03.005
- Author:
- Tang, Huipo; Rothery, Richard A.; Weiner, Joel H.
- Source:
- Biochimica et biophysica acta 2013 v.1827 no.6 pp. 730-737
- ISSN:
- 0005-2728
- Subject:
- Escherichia coli; amino acid substitution; antagonists; apoproteins; cell membranes; dimethyl sulfoxide; dimethylsulfoxide reductase; guanine; molybdates; molybdenum; protein subunits
- Abstract:
- ... We have investigated the final steps of complex iron–sulfur molybdoenzyme (CISM) maturation using Escherichia coli DMSO reductase (DmsABC) as a model system. The catalytic subunit of this enzyme, DmsA, contains an iron–sulfur cluster (FS0) and a molybdo-bis(pyranopterin guanine dinucleotide) cofactor (Mo-bisPGD). We have identified a variant of DmsA (Cys59Ser) that renders enzyme maturation sensit ...
- DOI:
- 10.1016/j.bbabio.2013.02.009
-
https://dx.doi.org/10.1016/j.bbabio.2013.02.009
- Author:
- Czapla, Monika; Cieluch, Ewelina; Borek, Arkadiusz; Sarewicz, Marcin; Osyczka, Artur
- Source:
- Biochimica et biophysica acta 2013 v.1827 no.6 pp. 751-760
- ISSN:
- 0005-2728
- Subject:
- Rhodobacter capsulatus; Rhodobacter sphaeroides; bacteria; cytochrome b; electron transfer; engineering
- Abstract:
- ... To address mechanistic questions about the functioning of dimeric cytochrome bc1 new genetic approaches have recently been developed. They were specifically designed to enable construction of asymmetrically-mutated variants suitable for functional studies. One approach exploited a fusion of two cytochromes b that replaced the separate subunits in the dimer. The fusion protein, built from two copie ...
- DOI:
- 10.1016/j.bbabio.2013.02.007
- PubMed:
- 23428397
- PubMed Central:
- PMC4330944
-
https://dx.doi.org/10.1016/j.bbabio.2013.02.007
- Author:
- Rehman, Ateeq Ur; Cser, Krisztián; Sass, László; Vass, Imre
- Source:
- Biochimica et biophysica acta 2013 v.1827 no.6 pp. 689-698
- ISSN:
- 0005-2728
- Subject:
- Synechocystis sp. PCC 6803; chlorophyll; deuterium oxide; electrodes; fluorescence; histidine; light intensity; lighting; models; mutants; oxygen; oxygen production; photoinhibition; photosystem II; singlet oxygen; trapping
- Abstract:
- ... Singlet oxygen production in intact cells of the cynobacterium Synechocystis 6803 was studied using chemical trapping by histidine, which leads to O2 uptake during illumination. The rate of O2 uptake, measured by a standard Clark-type electrode, is enhanced in the presence of D2O, which increases the lifetime of 1O2, and suppressed by the 1O2 quencher NaN3. Due to the limited mobility of 1O2 these ...
- DOI:
- 10.1016/j.bbabio.2013.02.016
-
https://dx.doi.org/10.1016/j.bbabio.2013.02.016
- Author:
- Koch-Koerfges, Abigail; Pfelzer, Nina; Platzen, Laura; Oldiges, Marco; Bott, Michael
- Source:
- Biochimica et biophysica acta 2013 v.1827 no.6 pp. 699-708
- ISSN:
- 0005-2728
- Subject:
- Corynebacterium glutamicum; acetates; adenosine triphosphate; aerobiosis; bacteria; biomass; electron transport chain; fumarate reductase (quinol); glucose; hydrolysis; mutants; oxygen; oxygen consumption; peptones; proton-motive force; protons; succinic acid
- Abstract:
- ... In this study a comparative analysis of three Corynebacterium glutamicum ATCC 13032 respiratory chain mutants lacking either the cytochrome bd branch (ΔcydAB), or the cytochrome bc1–aa3 branch (Δqcr), or both branches was performed. The lack of cytochrome bd oxidase was inhibitory only under conditions of oxygen limitation, whereas the absence of a functional cytochrome bc1–aa3 supercomplex led to ...
- DOI:
- 10.1016/j.bbabio.2013.02.004
-
https://dx.doi.org/10.1016/j.bbabio.2013.02.004
- Author:
- Reina, Simona; Magrì, Andrea; Lolicato, Marco; Guarino, Francesca; Impellizzeri, Agata; Maier, Elke; Benz, Roland; Ceccarelli, Matteo; De Pinto, Vito; Messina, Angela
- Source:
- Biochimica et biophysica acta 2013 v.1827 no.6 pp. 793-805
- ISSN:
- 0005-2728
- Subject:
- apoptosis; chimerism; metabolism; mitochondrial membrane; molecular dynamics; mutants; permeability
- Abstract:
- ... Voltage-dependent anion selective channel isoform1 maintains the permeability of the outer mitochondrial membrane. Its voltage-gating properties are relevant in bioenergetic metabolism and apoptosis. The N-terminal domain is suspected to be involved in voltage-gating, due to its peculiar localization. However this issue is still controversial. In this work we exchanged or deleted the β-strands tha ...
- DOI:
- 10.1016/j.bbabio.2013.03.007
-
https://dx.doi.org/10.1016/j.bbabio.2013.03.007
- Author:
- Postila, Pekka A.; Kaszuba, Karol; Sarewicz, Marcin; Osyczka, Artur; Vattulainen, Ilpo; Róg, Tomasz
- Source:
- Biochimica et biophysica acta 2013 v.1827 no.6 pp. 761-768
- ISSN:
- 0005-2728
- Subject:
- Rhodobacter capsulatus; electron transport chain; lipid bilayers; molecular dynamics; protein subunits; protons; sulfur
- Abstract:
- ... Cytochrome (cyt) bc1 complex, which is an integral part of the respiratory chain and related energy-conserving systems, has two quinone-binding cavities (Qo- and Qi-sites), where the substrate participates in electron and proton transfer. Due to its complexity, many of the mechanistic details of the cyt bc1 function have remained unclear especially regarding the substrate binding at the Qo-site. I ...
- DOI:
- 10.1016/j.bbabio.2013.02.005
-
https://dx.doi.org/10.1016/j.bbabio.2013.02.005
- Author:
- Tichy, Josef; Gardian, Zdenko; Bina, David; Konik, Peter; Litvin, Radek; Herbstova, Miroslava; Pain, Arnab; Vacha, Frantisek
- Source:
- Biochimica et biophysica acta 2013 v.1827 no.6 pp. 723-729
- ISSN:
- 0005-2728
- Subject:
- circular dichroism spectroscopy; solar radiation; electron microscopy; Rhodophyta; Miozoa; chlorophyll binding proteins; photosystem I; molecular weight; autotrophs; antennae; Bacillariophyceae
- Abstract:
- ... The structure and composition of the light harvesting complexes from the unicellular alga Chromera velia were studied by means of optical spectroscopy, biochemical and electron microscopy methods. Two different types of antennae systems were identified. One exhibited a molecular weight (18–19kDa) similar to FCP (fucoxanthin chlorophyll protein) complexes from diatoms, however, single particle anal ...
- DOI:
- 10.1016/j.bbabio.2013.02.002
-
https://dx.doi.org/10.1016/j.bbabio.2013.02.002
- Author:
- Wu, Wang; Ping, Wenli; Wu, Hanying; Li, Minchun; Gu, Dan; Xu, Yinong
- Source:
- Biochimica et biophysica acta 2013 v.1827 no.6 pp. 709-722
- ISSN:
- 0005-2728
- Subject:
- chlorophyll; chronic exposure; electron transfer; fluorescence; growth retardation; immunoblotting; phenotype; photostability; photosystem II; polyacrylamide gel electrophoresis; thylakoids; tobacco; transgenic plants
- Abstract:
- ... Monogalactosyldiacylglycerol (MGDG) is the most abundant lipid component of the thylakoid membrane. Although MGDG is believed to be important in sustaining the structure and function of the photosynthetic membrane, its exact role in photosynthesis in vivo requires further investigation. In this study, the transgenic tobacco plant M18, which has an MGDG deficiency of approximately 53%, and which co ...
- DOI:
- 10.1016/j.bbabio.2013.02.013
-
https://dx.doi.org/10.1016/j.bbabio.2013.02.013
- Author:
- Holzwarth, Alfred R.; Lenk, Dagmar; Jahns, Peter
- Source:
- Biochimica et biophysica acta 2013 v.1827 no.6 pp. 786-792
- ISSN:
- 0005-2728
- Subject:
- autotrophs; chlorophyll; fluorescence; lighting
- Abstract:
- ... Non-photochemical quenching (NPQ) protects photosynthetic organisms against photodamage by high light. One of the key measuring parameters for characterizing NPQ is the high-light induced decrease in chlorophyll fluorescence. The originally measured data are maximal fluorescence (Fm′) signals as a function of actinic illumination time (Fm′(t)). Usually these original data are converted into the so ...
- DOI:
- 10.1016/j.bbabio.2013.02.011
-
https://dx.doi.org/10.1016/j.bbabio.2013.02.011
- Author:
- Jennings, Robert C.; Zucchelli, Giuseppe; Santabarbara, Stefano
- Source:
- Biochimica et biophysica acta 2013 v.1827 no.6 pp. 779-785
- ISSN:
- 0005-2728
- Subject:
- absorption; corn; energy transfer; fluorescence; photosystem I; trapping; wavelengths
- Abstract:
- ... In the present paper the marked changes in photochemical trapping time over the absorption/fluorescence band of isolated PSI–LHCI are studied by means of time resolved fluorescence decay measurements. For emission at 680–690nm the effective trapping time is close to 17–18ps, and represents the effective trapping time from the bulk antenna. At wavelengths above 700nm the effective trapping time inc ...
- DOI:
- 10.1016/j.bbabio.2013.03.008
-
https://dx.doi.org/10.1016/j.bbabio.2013.03.008
- Author:
- Quintas, Pedro O.; Cepeda, Andreia P.; Borges, Nuno; Catarino, Teresa; Turner, David L.
- Source:
- Biochimica et biophysica acta 2013 v.1827 no.6 pp. 745-750
- ISSN:
- 0005-2728
- Subject:
- Methylophilus methylotrophus; bioremediation; cytochromes; electron transfer; electrostatic interactions; energy; ionic strength; mutants; pH; redox potential; sodium; thermodynamics
- Abstract:
- ... Multihaem cytochromes are essential to the energetics of organisms capable of bioremediation and energy production. The haems in several of these cytochromes have been discriminated thermodynamically and their individual rates of reduction by small electron donors were characterized. The kinetic characterization of individual haems used the Marcus theory of electron transfer and assumed that the r ...
- DOI:
- 10.1016/j.bbabio.2013.02.006
-
https://dx.doi.org/10.1016/j.bbabio.2013.02.006