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You searched for: Journal name Biochimica et biophysica acta Remove constraint Journal name: Biochimica et biophysica acta Publication Year 2011 Remove constraint Publication Year: 2011 Subject term spectroscopy Remove constraint Subject term: spectroscopy Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. Catalytic intermediates of cytochrome bd terminal oxidase at steady-state: Ferryl and oxy-ferrous species dominate

Author:
Vitaliy B. Borisov; Elena Forte; Paolo Sarti; Alessandro Giuffrè
Source:
Biochimica et biophysica acta 2011 v.1807 no.5 pp. 503-509
ISSN:
0005-2728
Subject:
Escherichia coli; absorption; cytochrome-c oxidase; dithiothreitol; heme; mammals; models; oxidation; oxygen; proton-motive force; spectroscopy; ubiquinones
Abstract:
... The cytochrome bd ubiquinol oxidase from Escherichia coli couples the exergonic two-electron oxidation of ubiquinol and four-electron reduction of O₂ to 2H₂O to proton motive force generation by transmembrane charge separation. The oxidase contains two b-type hemes (b₅₅₈ and b₅₉₅) and one heme d, where O₂ is captured and converted to water through sequential formation of a few intermediates. The s ...
DOI:
10.1016/j.bbabio.2011.02.007

2. Caudal-related homeodomain proteins CDX1/2 bind to DNA replication-related element binding factor

Author:
So Young Park; Mi Suk Jeong; Mi-Ae Yoo; Se Bok Jang
Source:
Biochimica et biophysica acta 2011 v.1814 no.12 pp. 1891-1899
ISSN:
1878-1454
Subject:
DNA; DNA-binding domains; Drosophila; Escherichia coli; amino acids; chromatography; computer software; fluorescence; genes; homeodomain proteins; intestinal mucosa; models; mutants; polypeptides; spectroscopy; surface plasmon resonance; transcription factors
Abstract:
... In the intestinal epithelium, the CDX1 and CDX2 homeodomain genes play proliferative and tumor suppressor roles, respectively. The transcription factor DNA replication-related element binding factor (DREF), is an 80kDa polypeptide homodimer that plays an important role in regulating cell proliferation-related genes. Homeodomain genes encode DNA-binding proteins that play crucial roles during devel ...
DOI:
10.1016/j.bbapap.2011.07.021

3. Characterization of photosystem II in transgenic tobacco plants with decreased iron superoxide dismutase

Author:
Yan Zhang; Shunhua Ding; Qingtao Lu; Zhipan Yang; Xiaogang Wen; Lixin Zhang; Congming Lu
Source:
Biochimica et biophysica acta 2011 v.1807 no.4 pp. 391-403
ISSN:
0005-2728
Subject:
D1 protein; Nicotiana tabacum; RNA interference; electron transfer; fluorescence; gels; genes; photosystem II; protein degradation; redox potential; spectroscopy; superoxide anion; superoxide dismutase; thermoluminescence; tobacco; transgenic plants
Abstract:
... Iron superoxide dismutases (FeSODs) play an important role in preventing the oxidative damage associated with photosynthesis. To investigate the mechanisms of FeSOD in protection against photooxidative stress, we obtained transgenic tobacco (Nicotiana tabacum) plants with severely decreased FeSOD by using a gene encoding tobacco chloroplastic FeSOD for the RNAi construct. Transgenic plants were hi ...
DOI:
10.1016/j.bbabio.2011.01.006

4. Coupling of protein and environment fluctuations

Author:
R.D. Young; P.W. Fenimore
Source:
Biochimica et biophysica acta 2011 v.1814 no.8 pp. 916-921
ISSN:
1878-1454
Subject:
differential scanning calorimetry; glass; models; myoglobin; powders; solvents; spectroscopy; temperature
Abstract:
... We review the concepts of protein dynamics developed over the last 35years and extend applications of the unified model of protein dynamics to heat flow and spatial fluctuations in hydrated myoglobin (Mb) powders. Differential scanning calorimetry (DSC) and incoherent neutron scattering (INS) data on hydration Mb powders are explained by the temperature-dependence of the hydration-shell βₕ process ...
DOI:
10.1016/j.bbapap.2011.05.005

5. Demonstration of the hepatocyte growth factor signaling pathway in the in vitro neuritogenic activity of chondroitin sulfate from ray fish cartilage

Author:
Taishi Hashiguchi; Takanari Kobayashi; Duriya Fongmoon; Ajaya Kumar Shetty; Shuji Mizumoto; Nobuyuki Miyamoto; Toshikazu Nakamura; Shuhei Yamada; Kazuyuki Sugahara
Source:
Biochimica et biophysica acta 2011 v.1810 no.4 pp. 406-413
ISSN:
0304-4165
Subject:
antibodies; cartilage; chondroitin sulfate; extracellular matrix; fish; glucuronic acid; hepatocyte growth factor; industrial wastes; nervous system; signal transduction; spectroscopy; surface plasmon resonance
Abstract:
... BACKGROUND: Chondroitin sulfate (CS) is a ubiquitous component of the cell surface and extracellular matrix and its sugar backbone consists of repeating disaccharide units: D-glucuronic acid (GlcUA)β1-3N-acetyl-D-galactosamine (GalNAc). Although CS participates in diverse biological processes such as growth factor signaling and the nervous system's development, the mechanism underlying the functio ...
DOI:
10.1016/j.bbagen.2011.01.001

6. Differential effects of short- and long-term high-fat diet feeding on hepatic fatty acid metabolism in rats

Author:
Jolita Ciapaite; Nicole M. van den Broek; Heleen te Brinke; Klaas Nicolay; Jeroen A. Jeneson; Sander M. Houten; Jeanine J. Prompers
Source:
Biochimica et biophysica acta 2011 v.1811 no.7-8 pp. 441-451
ISSN:
1388-1981
Subject:
adults; beta oxidation; biogenesis; carnitine; fatty acid metabolism; fatty acids; high fat diet; lipid peroxidation; liver; males; mitochondria; mitochondrial DNA; obesity; oxidation; oxidative stress; oxygen consumption; prices; rats; spectroscopy
Abstract:
... Imbalance in the supply and utilization of fatty acids (FA) is thought to contribute to intrahepatic lipid (IHL) accumulation in obesity. The aim of this study was to determine the time course of changes in the liver capacity to oxidize and store FA in response to high-fat diet (HFD). Adult male Wistar rats were fed either normal chow or HFD for 2.5weeks (short-term) and 25weeks (long-term). Short ...
DOI:
10.1016/j.bbalip.2011.05.005

7. Effect of protein–surfactant interactions on aggregation of β-lactoglobulin

Author:
Jon G. Hansted; Peter L. Wejse; Hans Bertelsen; Daniel E. Otzen
Source:
Biochimica et biophysica acta 2011 v.1814 no.5 pp. 713-723
ISSN:
1878-1454
Subject:
beta-lactoglobulin; calorimetry; cationic surfactants; cattle; chlorides; foods; melting point; micelles; neutralization; nonionic surfactants; sodium; sodium dodecyl sulfate; spectroscopy; sulfates; whey; whey protein
Abstract:
... The milk protein β-lactoglobulin (βLG) dominates the properties of whey aggregates in food products. Here we use spectroscopic and calorimetric techniques to elucidate how anionic, cationic and non-ionic surfactants interact with bovine βLG and modulate its heat-induced aggregation. Alkyl trimethyl ammonium chlorides (xTAC) strongly promote aggregation, while sodium alkyl sulfates (SxS) and alkyl ...
DOI:
10.1016/j.bbapap.2011.03.011

8. Energy transfer in an LH4-like light harvesting complex from the aerobic purple photosynthetic bacterium Roseobacter denitrificans

Author:
Dariusz M. Niedzwiedzki; Marcel Fuciman; Harry A. Frank; Robert E. Blankenship
Source:
Biochimica et biophysica acta 2011 v.1807 no.5 pp. 518-528
ISSN:
0005-2728
Subject:
Rhodobacter sphaeroides; Rhodopseudomonas palustris; Roseobacter denitrificans; absorption; ambient temperature; bacteria; carotenoids; energy transfer; fluorescence; light harvesting complex; pigments; protein structure; spectral analysis; spectroscopy; wavelengths
Abstract:
... A peripheral light-harvesting complex from the aerobic purple bacterium Roseobacter (R.) denitrificans was purified and its photophysical properties characterized. The complex contains two types of pigments, bacteriochlorophyll (BChl) a and the carotenoid (Car) spheroidenone and possesses unique spectroscopic properties. It appears to lack the B850 bacteriochlorophyll a Qy band that is typical for ...
DOI:
10.1016/j.bbabio.2011.03.004

9. Excited-state properties of the 16kDa red carotenoid protein from Arthrospira maxima

Author:
Pavel Chábera; Milan Durchan; Patrick M. Shih; Cheryl A. Kerfeld; Tomáš Polívka
Source:
Biochimica et biophysica acta 2011 v.1807 no.1 pp. 30-35
ISSN:
0005-2728
Subject:
Spirulina maxima; absorption; binding proteins; carotenoids; hydrogen bonding; hydrophilicity; hydrophobicity; photostability; plant proteins; solvents; spectroscopy
Abstract:
... We have studied spectroscopic properties of the 16kDa red carotenoid protein (RCP), which is closely related to the orange carotenoid protein (OCP) from cyanobacteria. Both proteins bind the same chromophore, the carotenoid 3′-hydroxyechinenone (hECN), and the major difference between the two proteins is lack of the C-terminal domain in the RCP; this results in exposure of part of the carotenoid. ...
DOI:
10.1016/j.bbabio.2010.08.013

10. Exploring methionine γ-lyase structure-function relationship via microspectrophotometry and X-ray crystallography

Author:
Luca Ronda; Natalia P. Bazhulina; Elena A. Morozova; Svetlana V. Revtovich; Vladimir O. Chekhov; Alexei D. Nikulin; Tatyana V. Demidkina; Andrea Mozzarelli
Source:
Biochimica et biophysica acta 2011 v.1814 no.6 pp. 834-842
ISSN:
1878-1454
Subject:
Citrobacter freundii; X-ray diffraction; absorption; active sites; ammonia; ammonium sulfate; antineoplastic agents; crystals; humans; methanethiol; methionine; microorganisms; neoplasms; pathogens; polyethylene glycol; protein structure; pyridoxal; spectral analysis; spectroscopy; structure-activity relationships
Abstract:
... Pyridoxal 5’-phosphate (PLP) dependent methionine γ-lyase catalyzes the breakdown of L-methionine to α-ketobutyric acid, methanethiol and ammonia. This enzyme, present in anaerobic microorganisms, has biomedical interest both for its activity as antitumor agent, depleting methionine supply in methionine-dependent cancers, and as target in the treatment of human pathogen infections, activating the ...
DOI:
10.1016/j.bbapap.2010.06.017

11. Exploring the inhibitory activity of short-chain phospholipids against amyloid fibrillogenesis of hen egg-white lysozyme

Author:
Steven S.-S. Wang; Ying-Tz Hung; Wen-Sing Wen; Keng-Chi Lin; Geng-Yuan Chen
Source:
Biochimica et biophysica acta 2011 v.1811 no.5 pp. 301-313
ISSN:
1388-1981
Subject:
amyloid; hens; human diseases; light scattering; lysozyme; molecular dynamics; pH; phospholipids; spectroscopy; transmission electron microscopy
Abstract:
... Amyloid fibrillogenesis is an important pathological feature of a group of degenerative human diseases. The 129-residue enzyme hen egg-white lysozyme has been shown to form fibrils in vitro at pH 2.0 and 55°C. In this research, using various spectroscopic techniques, light scattering, and transmission electron microscopy, we first examined the influence of short-chain phospholipids on the amyloid ...
DOI:
10.1016/j.bbalip.2011.02.003

12. Glass transition and dynamics in BSA–water mixtures over wide ranges of composition studied by thermal and dielectric techniques

Author:
A. Panagopoulou; A. Kyritsis; R. Sabater i Serra; J.L. Gómez Ribelles; N. Shinyashiki; P. Pissis
Source:
Biochimica et biophysica acta 2011 v.1814 no.12 pp. 1984-1996
ISSN:
1878-1454
Subject:
enthalpy; glass transition; ice; solutions; sorption; cooling; crystals; heat; mixtures; ambient temperature; differential scanning calorimetry; water content; pellets; sorption isotherms; crystallization; melting; bovine serum albumin; spectroscopy
Abstract:
... Protein–water dynamics in mixtures of water and a globular protein, bovine serum albumin (BSA), was studied over wide ranges of composition, in the form of solutions or hydrated solid pellets, by differential scanning calorimetry (DSC), thermally stimulated depolarization current technique (TSDC) and dielectric relaxation spectroscopy (DRS). Additionally, water equilibrium sorption isotherm (ESI) ...
DOI:
10.1016/j.bbapap.2011.07.014

13. How different oxidation states of crystalline myoglobin are influenced by X-rays

Author:
Hans-Petter Hersleth; K. Kristoffer Andersson
Source:
Biochimica et biophysica acta 2011 v.1814 no.6 pp. 785-796
ISSN:
1878-1454
Subject:
X-radiation; X-ray diffraction; absorption; active sites; annealing; crystal proteins; crystal structure; crystals; monitoring; myoglobin; oxidation; oxidative stress; oxygenases; peroxidases; peroxides; protein structure; spectroscopy; temperature
Abstract:
... X-ray induced radiation damage of protein crystals is well known to occur even at cryogenic temperatures. Redox active sites like metal sites seem especially vulnerable for these radiation-induced reductions. It is essential to know correctly the oxidation state of metal sites in protein crystal structures to be able to interpret the structure–function relation. Through previous structural studies ...
DOI:
10.1016/j.bbapap.2010.07.019

14. Inhibition of Rac activity alleviates lipopolysaccharide-induced acute pulmonary injury in mice

Author:
Hong-yi Yao; Lihua Chen; Chengyun Xu; Jirong Wang; Jiqiang Chen; Qiang-min Xie; Ximei Wu; Xiao-feng Yan
Source:
Biochimica et biophysica acta 2011 v.1810 no.7 pp. 666-674
ISSN:
0304-4165
Subject:
albumins; cell adhesion; cell cycle; cell growth; chemoattractants; cytoskeleton; gene expression; guanosinetriphosphatase; interleukin-1beta; intraperitoneal injection; lipopolysaccharides; lungs; messenger RNA; mice; myeloperoxidase; necrosis; neutrophils; permeability; pneumonia; reverse transcriptase polymerase chain reaction; spectroscopy; transcription (genetics); tumor necrosis factor-alpha
Abstract:
... BACKGROUND: Rac small GTPases play important roles in cytoskeleton and many cell functions including cell cycle, cell growth, cell adhesion and gene transcription. Here, we investigated the roles of Rac including Rac1 and Rac2 in lipopolysaccharide (LPS)-induced pulmonary injury. METHODS: After LPS was intratracheally instilled to lungs in mice, Rac, CDC42 and RhoA activation assay by pull-down an ...
DOI:
10.1016/j.bbagen.2011.03.020

15. Interaction of berberine, palmatine, coralyne, and sanguinarine to quadruplex DNA: A comparative spectroscopic and calorimetric study

Author:
Kakali Bhadra; Gopinatha Suresh Kumar
Source:
Biochimica et biophysica acta 2011 v.1810 no.4 pp. 485-496
ISSN:
0304-4165
Subject:
DNA; absorption; berberine; binding capacity; calorimetry; enthalpy; entropy; ethidium; fluorescence; heat production; humans; hydrophobicity; melting; sanguinarine; spectroscopy; telomeres
Abstract:
... BACKGROUND: Interaction of isoquinoline alkaloids berberine, palmatine, coralyne and sanguinarine with human telomeric quadruplex DNA, dAGGG(TTAGGG)₃, has been investigated and compared with ethidium. METHODS: Biophysical techniques such as absorption, fluorescence, circular dichroism, optical melting and microcalorimetry have been used. RESULTS: Absorption and fluorescence studies revealed noncoo ...
DOI:
10.1016/j.bbagen.2011.01.011

16. Mechanistic enzymology of serine palmitoyltransferase

Author:
Hiroko Ikushiro; Hideyuki Hayashi
Source:
Biochimica et biophysica acta 2011 v.1814 no.11 pp. 1474-1480
ISSN:
1878-1454
Subject:
biosynthesis; pyridoxal phosphate; sphingolipids; spectroscopy; enzymology; serine C-palmitoyltransferase
Abstract:
... Serine palmitoyltransferase, which is one of the α-oxamine synthase family enzymes, catalyzes the condensation reaction of L-serine and palmitoyl-CoA to form 3-ketodihydrosphingosine, the first and rate-determining step of the sphingolipid biosynthesis. As with other α-oxamine synthase family enzymes, the catalytic reaction is composed of multiple elementary steps, and the mechanism to control the ...
DOI:
10.1016/j.bbapap.2011.02.005

17. Pyridoxal-5′-phosphate as the catalyst for radical isomerization in reactions of PLP-dependent aminomutases

Author:
Perry A. Frey; George H. Reed
Source:
Biochimica et biophysica acta 2011 v.1814 no.11 pp. 1548-1557
ISSN:
1878-1454
Subject:
active sites; catalysts; crystal structure; enzymes; enzymology; free radicals; isomerization; lysine; ornithine; pyridoxal; spectroscopy
Abstract:
... PLP catalyzes the 1,2 shifts of amino groups in free radical-intermediates at the active sites of amino acid aminomutases. Free radical forms of the substrates are created upon H atom abstractions carried out by the 5′-deoxyadenosyl radical. In most of these enzymes, the 5′-deoxyadenosyl radical is generated by an iron–sulfur cluster-mediated reductive cleavage of S-adenosyl-(S)-methionine. Howeve ...
DOI:
10.1016/j.bbapap.2011.03.005

18. Retigeric acid B exerts antifungal effect through enhanced reactive oxygen species and decreased cAMP

Author:
Wen-Qiang Chang; Xiu-Zhen Wu; Ai-Xia Cheng; Li Zhang; Mei Ji; Hong-Xiang Lou
Source:
Biochimica et biophysica acta 2011 v.1810 no.5 pp. 569-576
ISSN:
0304-4165
Subject:
Candida albicans; Lobaria; adenosine triphosphate; antifungal properties; apoptosis; cell proliferation; confocal microscopy; cyclic AMP; cytotoxicity; death; energy; enzyme-linked immunosorbent assay; flow cytometry; fluorescence microscopy; human diseases; interphase; lichens; metabolites; necrosis; phenotype; phosphates; plasma membrane; reactive oxygen species; spectroscopy; transmission electron microscopy; triterpenoids; ultrastructure; yeasts
Abstract:
... BACKGROUND: Retigeric acid B (RAB), a triterpene acid isolated from Lobaria kurokawae exerts antifungal effect. The present study was designed to elucidate the underlying mechanisms by which RAB regulates the proliferation and cell death of Candida albicans. METHODS: We measured the metabolic activity of C. albicans with WST1 Cell Proliferation and Cytotoxicity Assay Kit, analyzed the cell cycle b ...
DOI:
10.1016/j.bbagen.2011.02.001

19. Structure and single crystal spectroscopy of Green Fluorescent Proteins

Author:
Stefano Bettati; Elisa Pasqualetto; Graziano Lolli; Barbara Campanini; Roberto Battistutta
Source:
Biochimica et biophysica acta 2011 v.1814 no.6 pp. 824-833
ISSN:
1878-1454
Subject:
crystal proteins; crystal structure; crystallization; crystals; green fluorescent protein; models; protein structure; spectral analysis; spectroscopy
Abstract:
... Usually, spectroscopic data on proteins in solution are interpreted at molecular level on the basis of the three-dimensional structures determined in the crystalline state. While it is widely recognized that the protein crystal structures are reliable models for the solution 3D structures, nevertheless it is also clear that sometimes the crystallization process can introduce some “artifacts” that ...
DOI:
10.1016/j.bbapap.2010.10.002

20. Structure-activity relationship of acridine derivatives to amyloid aggregation of lysozyme

Author:
Andrea Antosova; Beatrice Chelli; Eva Bystrenova; Katarina Siposova; Francesco Valle; Jan Imrich; Maria Vilkova; Pavol Kristian; Fabio Biscarini; Zuzana Gazova
Source:
Biochimica et biophysica acta 2011 v.1810 no.4 pp. 465-474
ISSN:
0304-4165
Subject:
Alzheimer disease; DNA; acridines; amyloid; atomic force microscopy; cell viability; cytotoxicity; depolymerization; inhibitory concentration 50; lysozyme; noninsulin-dependent diabetes mellitus; spectroscopy; structure-activity relationships
Abstract:
... BACKGROUND: Amyloid-related diseases (such as Alzheimer's disease or diabetes type II) are associated with self-assembly of protein into amyloid aggregates. METHODS: Spectroscopic and atomic force microscopy were used to determine the ability of acridines to affect amyloid aggregation of lysozyme. RESULTS: We have studied the effect of acridine derivatives on the amyloid aggregation of lysozyme to ...
DOI:
10.1016/j.bbagen.2011.01.007

21. Studies of photo-induced protein reactions by spectrally silent reaction dynamics detection methods: Applications to the photoreaction of the LOV2 domain of phototropin from Arabidopsis thaliana

Author:
Masahide Terazima
Source:
Biochimica et biophysica acta 2011 v.1814 no.8 pp. 1093-1105
ISSN:
1878-1454
Subject:
Arabidopsis thaliana; absorption; blue light; dissociation; hydrogen bonding; hydrophobic bonding; photoreceptors; refractive index; spectroscopy
Abstract:
... Biological function involves a series of chemical reactions of biological molecules, and during these reactions, there are numerous spectrally silent dynamic events that cannot be monitored by absorption or emission spectroscopic techniques. Such spectrally silent dynamics include changes in conformation, intermolecular interactions (hydrogen bonding, hydrophobic interactions), inter-protein inter ...
DOI:
10.1016/j.bbapap.2010.12.011

22. The kinetics of molecular oxygen migration in the isolated α chains of human hemoglobin as revealed by molecular dynamics simulations and laser kinetic spectroscopy

Author:
Sergei V. Lepeshkevich; Sergey A. Biziuk; Alexander M. Lemeza; Boris M. Dzhagarov
Source:
Biochimica et biophysica acta 2011 v.1814 no.10 pp. 1279-1288
ISSN:
1878-1454
Subject:
adults; amino acids; dissociation; hemoglobin; humans; hydrophobicity; models; molecular dynamics; oxygen; solutions; spectroscopy
Abstract:
... Bimolecular and geminate molecular oxygen (O₂) rebinding to isolated α chains of human adult hemoglobin in solutions is analyzed. Multiple extended molecular dynamics (MD) simulations of the O₂ migration within the protein after dissociation are described. Computational modeling is exploited to identify hydrophobic pockets within the αchains and internal O₂ migration pathways associated with the e ...
DOI:
10.1016/j.bbapap.2011.06.013

23. The role of pH on the thermodynamics and kinetics of muscle biochemistry: An in vivo study by ³¹P-MRS in patients with myo-phosphorylase deficiency

Author:
E. Malucelli; S. Iotti; D.N. Manners; C. Testa; A. Martinuzzi; B. Barbiroli; R. Lodi
Source:
Biochimica et biophysica acta 2011 v.1807 no.9 pp. 1244-1249
ISSN:
0005-2728
Subject:
acidification; acidosis; adenosine diphosphate; calves; creatine kinase; energy balance; exercise; glycogen; hydrolysis; in vivo studies; muscles; pH; patients; phosphocreatine; phosphorus; skeletal muscle; spectroscopy; thermodynamics
Abstract:
... In this study we assessed ΔG′ATP hydrolysis, cytosolic [ADP], and the rate of phosphocreatine recovery using Phosphorus Magnetic Resonance Spectroscopy in the calf muscle of a group of patients affected by glycogen myo-phosphorylase deficiency (McArdle disease). The goal was to ascertain whether and to what extent the deficit of the glycogenolytic pathway would affect the muscle energy balance. A ...
DOI:
10.1016/j.bbabio.2011.06.013

24. The stereochemistry of chlorophyll-c₃ from the haptophyte Emiliania huxleyi: The (13²R)-enantiomers of chlorophylls-c are exclusively selected as the photosynthetically active pigments in chromophyte algae

Author:
Tadashi Mizoguchi; Yuki Kimura; Taichi Yoshitomi; Hitoshi Tamiaki
Source:
Biochimica et biophysica acta 2011 v.1807 no.11 pp. 1467-1473
ISSN:
0005-2728
Subject:
Bacillariophyceae; Emiliania huxleyi; ambient temperature; autotrophs; biosynthesis; carotenoids; chlorophyll; dehydrogenation; enantiomers; high performance liquid chromatography; molecular models; natural selection; phylogeny; pigments; prokaryotic cells; spectroscopy; stereochemistry
Abstract:
... Chlorophyll(Chl)-c pigments in algae, diatoms and some prokaryotes are characterized by the fully conjugated porphyrin π-system as well as the acrylate residue at the 17-position. The precise structural characterization of Chl-c₃ from the haptophyte Emiliania huxleyi was performed. The conformations of the π-conjugated peripheral substituents, the 3-/8-vinyl, 7-methoxycarbonyl and 17-acrylate moie ...
DOI:
10.1016/j.bbabio.2011.07.008

25. Time-resolved single-turnover of caa₃ oxidase from Thermus thermophilus. Fifth electron of the fully reduced enzyme converts OH into EH state

Author:
Sergey A. Siletsky; Ilya Belevich; Nikolai P. Belevich; Tewfik Soulimane; Michael I. Verkhovsky
Source:
Biochimica et biophysica acta 2011 v.1807 no.9 pp. 1162-1169
ISSN:
0005-2728
Subject:
Thermus thermophilus; active sites; cytochrome-c oxidase; electron transfer; electrons; heme; spectral analysis; spectroscopy
Abstract:
... The oxidative part of the catalytic cycle of the caa₃-type cytochrome c oxidase from Thermus thermophilus was followed by time-resolved optical spectroscopy. Rate constants, chemical nature and the spectral properties of the catalytic cycle intermediates (Compounds A, P, F) reproduce generally the features typical for the aa₃-type oxidases with some distinctive peculiarities caused by the presence ...
DOI:
10.1016/j.bbabio.2011.05.006

26. Whole-cell biotransformation assay for investigation of the human drug metabolizing enzyme CYP3A7

Author:
Ina Neunzig; Călin-Aurel Drăgan; Maria Widjaja; Andrea E. Schwaninger; Frank T. Peters; Hans H. Maurer; Matthias Bureik
Source:
Biochimica et biophysica acta 2011 v.1814 no.1 pp. 161-167
ISSN:
1878-1454
Subject:
Schizosaccharomyces pombe; adults; adverse effects; antineoplastic agents; biotransformation; cytochrome P-450; hepatoma; humans; liver; luminescence; miconazole; pharmacokinetics; prasterone; spectroscopy; testosterone
Abstract:
... The cytochrome P450 isoform CYP3A7 (wildtype) is the major form of CYP in human fetal liver. Since it is not exclusively expressed in the fetus but also in a significant number of adults, CYP3A7 has been moving into the focus of investigation on adverse drug reactions and interindividual differences in drug metabolism in the last few years. In addition, CYP3A7 is overexpressed in hepatocellular ca ...
DOI:
10.1016/j.bbapap.2010.07.011