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1. 1α,25-Dihydroxyvitamin D₃ exerts tissue-specific effects on estrogen and androgen metabolism

Author:
Johan Lundqvist; Maria Norlin; Kjell Wikvall
Source:
Biochimica et biophysica acta 2011 v.1811 no.4 pp. 263-270
ISSN:
1388-1981
Subject:
antineoplastic agents; aromatase; breast neoplasms; endocrine-disrupting chemicals; enzyme activity; estradiol; gene expression; metabolism; models; prostatic neoplasms; screening; testosterone; tissues
Abstract:
... It is well-known that 1α,25-dihydroxyvitamin D₃ and analogs exert anti-proliferative and pro-differentiating effects and these compounds have therefore been proposed to be of potential use as anti-cancer agents. Due to its effects on aromatase gene expression and enzyme activity, 1α,25-dihydroxyvitamin D₃ has been proposed as an interesting substance in breast cancer treatment and prevention. In t ...
DOI:
10.1016/j.bbalip.2011.01.004

2. A combination method of chemical with enzyme reactions for identification of membrane proteins

Author:
Jeong-Eun Lee; Joseph Kwon; Moon-Chang Baek
Source:
Biochimica et biophysica acta 2011 v.1814 no.3 pp. 397-404
ISSN:
1878-1454
Subject:
acetic acid; chemical reactions; digestion; enzymatic reactions; kidneys; membrane proteins; new combination; peptides; rats; trypsin
Abstract:
... A simple method for effective analysis of various proteins has been developed, including membrane proteins, with LC–MS/MS, using CNBr and acetic acid cleavage in one reaction for the digestion of both the M/ and /D/ positions within the target proteins. This dual chemical reaction has been compared with traditional CNBr or an acid cleavage method using a rat kidney membrane fraction and it showed ...
DOI:
10.1016/j.bbapap.2010.12.001

3. A combined quantum chemical and crystallographic study on the oxidized binuclear center of cytochrome c oxidase

Author:
Ville R.I. Kaila; Esko Oksanen; Adrian Goldman; Dmitry A. Bloch; Michael I. Verkhovsky; Dage Sundholm; Mårten Wikström
Source:
Biochimica et biophysica acta 2011 v.1807 no.7 pp. 769-778
ISSN:
0005-2728
Subject:
X-radiation; X-ray diffraction; active sites; cytochrome-c oxidase; electron transport chain; oxygen; peroxides; spectral analysis; United States
Abstract:
... Cytochrome c oxidase (CcO) is the terminal enzyme of the respiratory chain. By reducing oxygen to water, it generates a proton gradient across the mitochondrial or bacterial membrane. Recently, two independent X-ray crystallographic studies ((Aoyama et al. Proc. Natl. Acad. Sci. USA 106 (2009) 2165–2169) and (Koepke et al. Biochim. Biophys. Acta 1787 (2009) 635–645)), suggested that a peroxide dia ...
DOI:
10.1016/j.bbabio.2010.12.016

4. A conserved isoleucine in the LOV1 domain of a novel phototropin from the marine alga Ostreococcus tauri modulates the dark state recovery of the domain

Author:
Sindhu Kandoth Veetil; Chitvan Mittal; Peeyush Ranjan; Suneel Kateriya
Source:
Biochimica et biophysica acta 2011 v.1810 no.7 pp. 675-682
ISSN:
0304-4165
Subject:
crosslinking; proteins; aqueous solutions; serine; valine; absorption; mutation; glutaraldehyde; blue light; genes; isoleucine; spectral analysis; fluorescence emission spectroscopy; autotrophs
Abstract:
... BACKGROUND: Phototropins are UV-A/blue light receptor proteins with two LOV (Light-Oxygen-Voltage) sensor domains at their N terminus and a kinase domain at the C-terminus in photoautotrophic organisms. This is the first research report of a canonical phototropin from marine algae Ostreococcus tauri. METHODS: We synthesized core LOV1 (OtLOV1) domain-encoding portion of the phototropin gene of O. t ...
DOI:
10.1016/j.bbagen.2011.04.008

5. A fraction of the transcription factor TAF15 participates in interactions with a subset of the spliceosomal U1 snRNP complex

Author:
Michael Leichter; Marija Marko; Vassiliki Ganou; Meropi Patrinou-Georgoula; László Tora; Apostolia Guialis
Source:
Biochimica et biophysica acta 2011 v.1814 no.12 pp. 1812-1824
ISSN:
1878-1454
Subject:
crosslinking; transcription factors; protein-protein interactions; alpha-amanitin; gene expression; precipitin tests; small nuclear RNA; spliceosomes; DNA-directed RNA polymerase; autoantibodies
Abstract:
... RNA/ssDNA-binding proteins comprise an emerging class of multifunctional proteins with an anticipated role in coupling transcription with RNA processing. We focused here on the highly related transcription factors of the TET sub-class: TLS/FUS, EWS and in particular the least studied member TAF15. An extensive array of immunoprecipitation studies on differentially extracted HeLa nuclei revealed th ...
DOI:
10.1016/j.bbapap.2011.09.008

6. A heterogeneous tag-attachment to the homodimeric type 1 photosynthetic reaction center core protein in the green sulfur bacterium Chlorobaculum tepidum

Author:
Chihiro Azai; Kwang Kim; Toru Kondo; Jiro Harada; Shigeru Itoh; Hirozo Oh-oka
Source:
Biochimica et biophysica acta 2011 v.1807 no.7 pp. 803-812
ISSN:
0005-2728
Subject:
Chlorobium tepidum; DNA; absorption; bacteria; chromatography; electron paramagnetic resonance spectroscopy; electron transfer; gene duplication; genes; histidine; homologous recombination; mutants; site-directed mutagenesis; solubilization; spectral analysis; sulfur
Abstract:
... The 6xHis-tag-pscA gene, which was genetically engineered to express N-terminally histidine (His)-tagged PscA, was inserted into a coding region of the recA gene in the green sulfur bacterium Chlorobaculum tepidum (C. tepidum). Although the inactivation of the recA gene strongly suppressed a homologous recombination in C. tepidum genomic DNA, the mutant grew well under normal photosynthetic condit ...
DOI:
10.1016/j.bbabio.2011.03.007

7. A kinetic model of non-photochemical quenching in cyanobacteria

Author:
Maxim Y. Gorbunov; Fedor I. Kuzminov; Victor V. Fadeev; John Dongun Kim; Paul G. Falkowski
Source:
Biochimica et biophysica acta 2011 v.1807 no.12 pp. 1591-1599
ISSN:
0005-2728
Subject:
Cyanobacteria; absorption; activation energy; autotrophs; carotenoids; energy; eukaryotic cells; heat; isomerization; models; photosystem II; phycobilisome; proline
Abstract:
... High light poses a threat to oxygenic photosynthetic organisms. Similar to eukaryotes, cyanobacteria evolved a photoprotective mechanism, non-photochemical quenching (NPQ), which dissipates excess absorbed energy as heat. An orange carotenoid protein (OCP) has been implicated as a blue-green light sensor that induces NPQ in cyanobacteria. Discovered in vitro, this process involves a light-induced ...
DOI:
10.1016/j.bbabio.2011.08.009

8. A method to assess the migration properties of cell-derived microparticles within a living tissue

Author:
Thang Q. Hoang; Christine Rampon; Jean-Marie Freyssinet; Sophie Vriz; Danièle Kerbiriou-Nabias
Source:
Biochimica et biophysica acta 2011 v.1810 no.9 pp. 863-866
ISSN:
0304-4165
Subject:
Danio rerio; T-lymphocytes; apoptosis; calcium; eggs; extracellular space; fluorescence; humans; injection site; kidneys; plasma membrane; sialic acids
Abstract:
... BACKGROUND: Cells undergoing activation or apoptosis exhibit plasma membrane changes, leading to the formation of shed vesicles (microparticles, MP). Although their effects on recipient cells in vitro, and their ability to support inflammatory or thrombotic events in the circulation have been studied, the spreading of such vesicles in tissues is still elusive. Our aim was to set up a method to exa ...
DOI:
10.1016/j.bbagen.2011.05.003

9. A new human dyskerin isoform with cytoplasmic localization

Author:
Alberto Angrisani; Mimmo Turano; Lorella Paparo; Concetta Di Mauro; Maria Furia
Source:
Biochimica et biophysica acta 2011 v.1810 no.12 pp. 1361-1368
ISSN:
0304-4165
Subject:
alternative splicing; bioinformatics; bone marrow; cell adhesion; cell proliferation; clones; genes; humans; immunoblotting; immunocytochemistry; messenger RNA; pathogenesis; reverse transcriptase polymerase chain reaction; stem cells; telomerase; telomeres
Abstract:
... BACKGROUND: The human DKC1 gene is causative of X-linked dyskeratosis congenita (X-DC), a syndrome characterized by mucocutaneous features, bone marrow failure, tumor susceptibility, perturbation of stem cell function, and premature aging. DKC1 is thought to produce a single protein, named dyskerin, which shows strict nucleolar localization and participates in at least two distinct nuclear functio ...
DOI:
10.1016/j.bbagen.2011.07.012

10. A novel GRAIL E3 ubiquitin ligase promotes environmental salinity tolerance in euryhaline tilapia

Author:
Diego F. Fiol; Enio Sanmarti; Andreana H. Lim; Dietmar Kültz
Source:
Biochimica et biophysica acta 2011 v.1810 no.4 pp. 439-445
ISSN:
0304-4165
Subject:
Oreochromis mossambicus; Western blotting; adaptive immunity; apoptosis; cell viability; epithelium; euryhaline species; fish; gene expression regulation; genes; immunohistochemistry; lymphocytes; messenger RNA; nucleotide sequences; osmoregulation; osmotic stress; osmotolerance; proteinases; salinity; salt stress; salt tolerance; sequence homology; stress response; tissue distribution; ubiquitin-protein ligase
Abstract:
... BACKGROUND: Tilapia (Oreochromis mossambicus) are euryhaline fishes capable of tolerating large salinity changes. In a previous study aimed to identify genes involved in osmotolerance, we isolated an mRNA sequence with similarity to GRAIL (Gene Related to Anergy In Lymphocytes), which is a critical regulator of adaptive immunity and development. Tilapia GRAIL contains a PA (protease associated) do ...
DOI:
10.1016/j.bbagen.2010.11.005

11. A novel approach for the characterisation of proteoglycans and biosynthetic enzymes in a snail model

Author:
Tarsis F. Gesteira; Vivien Jane Coulson-Thomas; Fernando T. Ogata; Eduardo H.C. Farias; Renan P. Cavalheiro; Marcelo A. de Lima; Gabriel L.A. Cunha; Ernesto S. Nakayasu; Igor C. Almeida; Leny Toma; Helena B. Nader
Source:
Biochimica et biophysica acta 2011 v.1814 no.12 pp. 1862-1869
ISSN:
1878-1454
Subject:
Achatina fulica; biosynthesis; enzymes; evolution; glycosaminoglycans; immunohistochemistry; methodology; models; polymers; proteoglycans; proteomics; snails; tissues; uronic acids
Abstract:
... Proteoglycans encompass a heterogeneous group of glycoconjugates where proteins are substituted with linear, highly negatively charged glycosaminoglycan chains. Sulphated glycosaminoglycans are ubiquitous to the animal kingdom of the Eukarya domain. Information on the distribution and characterisation of proteoglycans in invertebrate tissues is limited and restricted to a few species. By the use o ...
DOI:
10.1016/j.bbapap.2011.07.024

12. A novel approach for the purification and proteomic analysis of pathogenic immunoglobulin free light chains from serum

Author:
Francesca Lavatelli; Francesca Brambilla; Veronica Valentini; Paola Rognoni; Simona Casarini; Dario Di Silvestre; Vittorio Perfetti; Giovanni Palladini; Gabriele Sarais; Pierluigi Mauri; Giampaolo Merlini
Source:
Biochimica et biophysica acta 2011 v.1814 no.3 pp. 409-419
ISSN:
1878-1454
Subject:
agarose; amyloidosis; antibodies; blood serum; bone marrow; dimerization; immunoblotting; immunoglobulin light chains; mass spectrometry; myeloma; oxidation; pathogenicity; patients; polyacrylamide gel electrophoresis; post-translational modification; precipitin tests; proteomics; tryptophan; two-dimensional gel electrophoresis
Abstract:
... An excess of circulating monoclonal free immunoglobulin light chains (FLC) is common in plasma cell disorders. A subset of FLC, as amyloidogenic ones, possess intrinsic pathogenicity. Because of their complex purification, little is known on the biochemical features of serum FLC, possibly related to their pathogenic spectrum. We developed an immunopurification approach to isolate serum FLC from pa ...
DOI:
10.1016/j.bbapap.2010.12.012

13. A novel retinoic acid analogue, 7-hydroxy retinoic acid, isolated from cyanobacteria

Author:
Kunimitsu Kaya; Fujio Shiraishi; Hideaki Uchida; Tomoharu Sano
Source:
Biochimica et biophysica acta 2011 v.1810 no.4 pp. 414-419
ISSN:
0304-4165
Subject:
Microcystis aeruginosa; Spirulina; animal tissues; fluorescent lighting; high performance liquid chromatography; isolation techniques; isomerization; isomers; mass spectrometry; metabolites; retinoic acid; two hybrid system techniques; ultraviolet radiation; vitamin A
Abstract:
... BACKGROUND: All-trans retinoic acid (RA) is a low-molecular compound derived from vitamin A. It induces events in various ways by binding with the retinoic acid receptor (RAR), a nuclear receptor, in animal cells. RA and its metabolites have been found in animal tissues. In this paper, we report a novel RA analogue found in cyanobacterial cells, describe the method for its isolation, and compare i ...
DOI:
10.1016/j.bbagen.2010.11.009

14. A novel subfamily of mitochondrial dicarboxylate carriers from Drosophila melanogaster: Biochemical and computational studies

Author:
Domenico Iacopetta; Marianna Madeo; Gianluca Tasco; Chiara Carrisi; Rosita Curcio; Emanuela Martello; Rita Casadio; Loredana Capobianco; Vincenza Dolce
Source:
Biochimica et biophysica acta 2011 v.1807 no.3 pp. 251-261
ISSN:
0005-2728
Subject:
Drosophila melanogaster; amino acid sequences; developmental stages; genome; mammals; mitochondria; mitochondrial membrane; phosphates; protein synthesis; proteins; screening; substrate specificity; yeasts
Abstract:
... The dicarboxylate carrier is an important member of the mitochondrial carrier family, which catalyzes an electroneutral exchange across the inner mitochondrial membrane of dicarboxylates for inorganic phosphate and certain sulfur-containing compounds. Screening of the Drosophila melanogaster genome revealed the presence of a mitochondrial carrier subfamily constituted by four potential homologs of ...
DOI:
10.1016/j.bbabio.2010.11.013

15. A novel vanadium transporter of the Nramp family expressed at the vacuole of vanadium-accumulating cells of the ascidian Ascidia sydneiensis samea

Author:
Tatsuya Ueki; Nobuaki Furuno; Hitoshi Michibata
Source:
Biochimica et biophysica acta 2011 v.1810 no.4 pp. 457-464
ISSN:
0304-4165
Subject:
H-transporting ATP synthase; Western blotting; antiporters; blood groups; copper; electrochemistry; ions; iron; manganese; oocytes; pH; rats; vacuoles; vanadium; zinc
Abstract:
... BACKGROUND: Vanadium is an essential transition metal in biological systems. Several key proteins related to vanadium accumulation and its physiological function have been isolated, but no vanadium ion transporter has yet been identified. METHODS: We identified and cloned a member of the Nramp/DCT family of membrane metal transporters (AsNramp) from the ascidian Ascidia sydneiensis samea, which ca ...
DOI:
10.1016/j.bbagen.2010.12.006

16. A physiologically-based kinetic model for the prediction of plasma cholesterol concentrations in the mouse

Author:
Niek C.A. van de Pas; Ruud A. Woutersen; Ben van Ommen; Ivonne M.C.M. Rietjens; Albert A. de Graaf
Source:
Biochimica et biophysica acta 2011 v.1811 no.5 pp. 333-342
ISSN:
1388-1981
Subject:
cholesterol metabolism; genes; high density lipoprotein cholesterol; knockout mutants; liver; low density lipoprotein cholesterol; mice; models; prediction
Abstract:
... The LDL cholesterol (LDL-C) and HDL cholesterol (HDL-C) concentrations are determined by the activity of a complex network of reactions in several organs. Physiologically-based kinetic (PBK) computational models can be used to describe these different reactions in an integrated, quantitative manner. A PBK model to predict plasma cholesterol levels in the mouse was developed, validated, and analyze ...
DOI:
10.1016/j.bbalip.2011.02.002

17. A screen for potential ferredoxin electron transfer partners uncovers new, redox dependent interactions

Author:
G.T. Hanke; Y. Satomi; K. Shinmura; T. Takao; T. Hase
Source:
Biochimica et biophysica acta 2011 v.1814 no.2 pp. 366-374
ISSN:
1878-1454
Subject:
Synechocystis; electron transfer; electron transport chain; electrons; energy transfer; matrix-assisted laser desorption-ionization mass spectrometry; photosynthesis; phycobilisome; recombinant proteins; regulatory proteins
Abstract:
... Ferredoxin (Fd) is the primary soluble acceptor at the end of the photosynthetic electron transport chain, and is known to directly transfer electrons to a wide range of proteins for use in metabolism and regulatory processes. We have conducted a screen to identify new putative Fd interaction partners in the cyanobacteria Synechocystis sp. PCC 6803 using Fd-chromatography in combination with MALDI ...
DOI:
10.1016/j.bbapap.2010.09.011

18. A theoretical investigation of the functional role of the axial methionine ligand of the CuA site in cytochrome c oxidase

Author:
Jiyoung Kang; Hiori Kino; Masaru Tateno
Source:
Biochimica et biophysica acta 2011 v.1807 no.10 pp. 1314-1327
ISSN:
0005-2728
Subject:
cattle; copper; cytochrome-c oxidase; electron transfer; energy; enzyme activity; ions; ligands; methionine; models; proton pump; redox potential
Abstract:
... The functional roles of the amino acid residues of the CuA site in bovine cytochrome c oxidase (CcO) were investigated by utilizing hybrid quantum mechanics (QM)/molecular mechanics (MM) calculations. The energy levels of the molecular orbitals (MOs) involving Cu dzₓ orbitals unexpectedly increased, as compared with those found previously with a simplified model system lacking the axial Met residu ...
DOI:
10.1016/j.bbabio.2011.06.014

19. A thermostable esterase from Thermoanaerobacter tengcongensis opening up a new family of bacterial lipolytic enzymes

Author:
Lang Rao; Yanfen Xue; Cheng Zhou; Jin Tao; Gang Li; Jian R. Lu; Yanhe Ma
Source:
Biochimica et biophysica acta 2011 v.1814 no.12 pp. 1695-1702
ISSN:
1570-9639
Subject:
Escherichia coli; Thermoanaerobacter tengcongensis; cysteine; esterases; genes; hexanoic acid; lipolysis; new family; pH; phylogeny; sequence alignment; site-directed mutagenesis; thermal stability; triacetin
Abstract:
... An unidentified α/β hydrolase gene lipA3 from thermostable eubacterium species Thermoanaerobacter tengcongensis MB4 was cloned and heterologously expressed by Escherichia coli BL21(DE3)pLysS. The purified recombinant enzyme EstA3 turned out to be a monomeric thermostable esterase with optimal activity at 70°C and pH 9.5. The enzyme showed lipolytic activity towards a wide range of ester substrates ...
DOI:
10.1016/j.bbapap.2011.08.013

20. A two-state stabilization-change mechanism for proton-pumping complex I

Author:
Ulrich Brandt
Source:
Biochimica et biophysica acta 2011 v.1807 no.10 pp. 1364-1369
ISSN:
0005-2728
Subject:
X-ray diffraction; electron transfer; energy transfer; membrane proteins; models; oxidative phosphorylation; stoichiometry
Abstract:
... Despite its central function in oxidative phosphorylation, the molecular mechanism of proton pumping respiratory complex I is still elusive. In recent years, considerable progress has been made towards understanding structure/function relationships in this very large and complicated membrane protein complex. Last year X-ray crystallographic analysis of bacterial and mitochondrial complex I provide ...
DOI:
10.1016/j.bbabio.2011.04.006

21. AICAR inhibits cancer cell growth and triggers cell-type distinct effects on OXPHOS biogenesis, oxidative stress and Akt activation

Author:
Caroline Jose; Etienne Hébert-Chatelain; Nadège Bellance; Anaïs Larendra; Melser Su; Karine Nouette-Gaulain; Rodrigue Rossignol
Source:
Biochimica et biophysica acta 2011 v.1807 no.6 pp. 707-718
ISSN:
0005-2728
Subject:
AMP-activated protein kinase; adenosine triphosphate; agonists; apoptosis; biogenesis; cell growth; cell proliferation; cell viability; energy; humans; mechanism of action; membrane potential; mitochondrial membrane; myoblasts; neoplasm cells; neurons; oxidative phosphorylation; oxidative stress
Abstract:
... The AMP-activated protein kinase agonist AICAR mimics a low intracellular energy state and inhibits the proliferation of cancer cells by different mechanisms, which may depend on the bioenergetic signature of these cells. AICAR can also stimulate mitochondrial biogenesis in myoblasts, neurons and HeLa cells. Yet, whether the reactivation of oxidative phosphorylation biogenesis by AICAR contributes ...
DOI:
10.1016/j.bbabio.2010.12.002

22. ATP synthase superassemblies in animals and plants: Two or more are better

Author:
Holger Seelert; Norbert A. Dencher
Source:
Biochimica et biophysica acta 2011 v.1807 no.9 pp. 1185-1197
ISSN:
0005-2728
Subject:
H+/K+-exchanging ATPase; H-transporting ATP synthase; adenine; chloroplasts; electrophoresis; energy conversion; energy metabolism; mammals; pathophysiology; plants (botany); protein-protein interactions; proteins
Abstract:
... ATP synthases are part of the sophisticated cellular metabolic network and therefore multiple interactions have to be considered. As discussed in this review, ATP synthases form various supramolecular structures. These include dimers and homooligomeric species. But also interactions with other proteins, particularly those involved in energy conversion exist. The supramolecular assembly of the ATP ...
DOI:
10.1016/j.bbabio.2011.05.023

23. ATP-binding cassette G5/G8 deficiency causes hypertriglyceridemia by affecting multiple metabolic pathways

Author:
Jesús Méndez-González; Josep Julve; Noemí Rotllan; Gemma Llaverias; Francisco Blanco-Vaca; Joan Carles Escolà-Gil
Source:
Biochimica et biophysica acta 2011 v.1811 no.12 pp. 1186-1193
ISSN:
1388-1981
Subject:
biochemical pathways; diet; gene expression; glycerol; heterozygosity; hypertriglyceridemia; insulin resistance; lipoprotein lipase; liver; metabolism; mice; mutation; oleic acid; phytosterols; secretion; transporters
Abstract:
... Mutations in ABCG5 or ABCG8 transporters are responsible for sitosterolemia, an autosomal recessive disease characterized by the accumulation of plant sterols. The aim of this study was to investigate the effects of ABCG5 and ABCG8 deficiency on TG metabolism in mice. Experiments were carried out in wild-type (G5/G8+/+) mice, mice heterozygous for ABCG5 and ABCG8 deficiency (G5/G8+/−) and ABCG5/G8 ...
DOI:
10.1016/j.bbalip.2011.07.019

24. Abnormal subcellular localization of AQP5 and downregulated AQP5 protein in parotid glands of streptozotocin-induced diabetic rats

Author:
Di Wang; Zhenfang Yuan; Noriko Inoue; Gota Cho; Masayuki Shono; Yasuko Ishikawa
Source:
Biochimica et biophysica acta 2011 v.1810 no.5 pp. 543-554
ISSN:
0304-4165
Subject:
Western blotting; agonists; animal disease models; cytoplasm; diabetes mellitus; gene expression; immunocytochemistry; immunohistochemistry; insulin; intravenous injection; messenger RNA; octoxynol; parotid gland; plasma membrane; quantitative polymerase chain reaction; rats; solubility
Abstract:
... BACKGROUND: The mechanisms underlying diabetic xerostomia have not been clarified in relation with aquaporin-5 (AQP5) subcellular localization in salivary glands. METHODS: Western blotting, real-time PCR, and immunocytochemistry were used to analyse AQP5 protein levels and mRNA expression. AQP5 protein levels were measured in the apical plasma membrane (APM) and detergent-insoluble fraction prepar ...
DOI:
10.1016/j.bbagen.2011.01.013

25. Absence of mitochondrial uncoupling protein 3: Effect on thymus and spleen in the fed and fasted mice

Author:
Orlagh M. Kelly; Richard K. Porter
Source:
Biochimica et biophysica acta 2011 v.1807 no.9 pp. 1064-1074
ISSN:
0005-2728
Subject:
apoptosis; confocal microscopy; gene expression; mice; mitochondria; oxygen consumption; spleen; splenocytes; starvation; suckling; thymocytes; thymus gland; weaning
Abstract:
... Mitochondrial uncoupling protein 3 (UCP3) is constitutively expressed in mitochondria from thymus and spleen of mice, and confocal microscopy has been used to visualize UCP3 in situ in mouse thymocytes. UCP3 is present in mitochondria of thymus and spleen up to at least 16weeks after birth, but levels decrease by a half in thymus and a fifth in spleen after three weeks, probably reflecting the suc ...
DOI:
10.1016/j.bbabio.2011.06.002

26. Acridine and quindoline oligomers linked through a 4-aminoproline backbone prefer G-quadruplex structures

Author:
Rubén Ferreira; Roberto Artali; Josep Farrera-Sinfreu; Fernando Albericio; Miriam Royo; Ramon Eritja; Stefania Mazzini
Source:
Biochimica et biophysica acta 2011 v.1810 no.8 pp. 769-776
ISSN:
0304-4165
Subject:
DNA; antineoplastic agents; aromatic compounds; binding properties; dialysis; ligands; models; molecular dynamics; nuclear magnetic resonance spectroscopy; oncogenes; promoter regions; telomeres
Abstract:
... BACKGROUND: DNA-intercalating drugs are planar molecules with several fused aromatic rings that form stacks between DNA base pairs, reducing the opening and unwinding of the double helix. Recently, interest on intercalating agents has moved in the search for new ligands to G-quadruplex structures. METHODS: The DNA binding properties of 4-aminoproline oligomers functionalized with one, two or three ...
DOI:
10.1016/j.bbagen.2011.04.013

27. Acylated and unacylated ghrelin attenuate isoproterenol-induced lipolysis in isolated rat visceral adipocytes through activation of phosphoinositide 3-kinase γ and phosphodiesterase 3B

Author:
Alessandra Baragli; Corrado Ghè; Elisa Arnoletti; Riccarda Granata; Ezio Ghigo; Giampiero Muccioli
Source:
Biochimica et biophysica acta 2011 v.1811 no.6 pp. 386-396
ISSN:
1388-1981
Subject:
adipocytes; apoptosis; cardiomyocytes; cyclic AMP; forskolin; ghrelin; islets of Langerhans; isozymes; lipolysis; mitogen-activated protein kinase; phosphatidylinositol 3-kinase; phosphorylation; rats
Abstract:
... The acylated peptide ghrelin (AG) and its endogenous non-acylated isoform (UAG) protect cardiomyocytes, pancreatic β-cells, and preadipocytes from apoptosis, and induce preadipocytes differentiation into adipocytes. These events are mediated by AG and UAG binding to a still unidentified receptor, which determines the activation of phosphoinositide 3-kinase (PI3K), protein kinase B (AKT), and mitog ...
DOI:
10.1016/j.bbalip.2011.03.001

28. Adenine nucleotide translocase 2 is a key mitochondrial protein in cancer metabolism

Author:
Arnaud Chevrollier; Dominique Loiseau; Pascal Reynier; Georges Stepien
Source:
Biochimica et biophysica acta 2011 v.1807 no.6 pp. 562-567
ISSN:
0005-2728
Subject:
adenine; adenosine diphosphate; adenosine triphosphate; apoptosis; brain; carcinogenesis; cell proliferation; cell viability; cytosol; germ cells; glycolysis; humans; membrane potential; mitochondria; mitochondrial membrane; neoplasms; oxidative phosphorylation; tissues
Abstract:
... Adenine nucleotide translocase (ANT), a mitochondrial protein that facilitates the exchange of ADP and ATP across the mitochondrial inner membrane, plays an essential role in cellular energy metabolism. Human ANT presents four isoforms (ANT1-4), each with a specific expression depending on the nature of the tissue, cell type, developmental stage and status of cell proliferation. Thus, ANT1 is spec ...
DOI:
10.1016/j.bbabio.2010.10.008

29. Adrenodoxin: The archetype of vertebrate-type [2Fe–2S] cluster ferredoxins

Author:
Kerstin Maria Ewen; Michael Kleser; Rita Bernhardt
Source:
Biochimica et biophysica acta 2011 v.1814 no.1 pp. 111-125
ISSN:
1878-1454
Subject:
amino acid sequences; biosynthesis; cytochrome P-450; electron transfer; ferredoxin-NADP reductase; ferredoxins; mammals; protein tertiary structure; protein-protein interactions; steroid hormones
Abstract:
... Adrenodoxin is probably the best characterized member of the vertebrate-type [2Fe–2S]-cluster ferredoxins. It has been in the spotlight of scientific interest for many years due to its essential role in mammalian steroid hormone biosynthesis, where it acts as electron mediator between the NADPH-dependent adrenodoxin reductase and several mitochondrial cytochromes P450. In this review we will focus ...
DOI:
10.1016/j.bbapap.2010.06.003

30. Adsorption of low-density lipoprotein, its oxidation, and subsequent binding of specific recombinant antibodies: An in situ ellipsometric study

Author:
Maria M. Stollenwerk; Olof Svensson; Alexandru Schiopu; Bo Jansson; Thomas Arnebrant; Gunilla Nordin Fredrikson
Source:
Biochimica et biophysica acta 2011 v.1810 no.2 pp. 211-217
ISSN:
0304-4165
Subject:
adsorption; apolipoprotein B-100; atherogenesis; binding sites; desorption; epitopes; humans; immune system; low density lipoprotein; monitoring; oxidation; recombinant antibodies; silica
Abstract:
... BACKGROUND: Low-density lipoprotein (LDL) particles accumulate in the arterial wall and become oxidized during atherogenesis, leading to the formation of atherosclerotic plaques. The major protein of the LDL particle, apolipoprotein B-100 (apoB-100), becomes fragmented during oxidation and a target for the immune system. METHODS: In this study we used in situ ellipsometry to monitor the adsorption ...
DOI:
10.1016/j.bbagen.2010.10.006

31. Agaritine from Agaricus blazei Murrill induces apoptosis in the leukemic cell line U937

Author:
Hidehiko Akiyama; Masahiro Endo; Taei Matsui; Itsurou Katsuda; Nobuhiko Emi; Yasuko Kawamoto; Takaaki Koike; Hidehiko Beppu
Source:
Biochimica et biophysica acta 2011 v.1810 no.5 pp. 519-525
ISSN:
0304-4165
Subject:
Agaricus subrufescens; DNA; DNA fragmentation; Western blotting; agarose; antigens; antineoplastic agents; apoptosis; caspase-3; caspase-8; cytochrome c; flow cytometry; gel electrophoresis; leukemia; mechanism of action; mitochondria; patients; phosphatidylserines
Abstract:
... BACKGROUND: Agaricus blazei Murrill (ABM) has been shown to exhibit immunostimulatory and anti-cancer activities; however, its mechanism of action is poorly understood. We recently found that the diffusible fraction of hot-water extract of ABM exhibits anti-tumor activity toward leukemic cells, and identified it as agaritine, a hydrazine-containing compound. In the present study, we examined the m ...
DOI:
10.1016/j.bbagen.2011.02.010

32. Aggregation and structural changes of αS₁-, β- and κ-caseins induced by homocysteinylation

Author:
Yulia Y. Stroylova; Jaroslaw Zimny; Reza Yousefi; Jean-Marc Chobert; Hieronim Jakubowski; Vladimir I. Muronetz; Thomas Haertlé
Source:
Biochimica et biophysica acta 2011 v.1814 no.10 pp. 1234-1245
ISSN:
1570-9639
Subject:
cattle; disulfide bonds; fluorescence; homocysteine; humans; kappa-casein; micelles; models; physicochemical properties; temperature
Abstract:
... Elevated homocysteine levels are resulting in N-homocysteinylation of lysyl residues in proteins and they correlate with a number of human pathologies. However, the role of homocysteinylation of lysyl residues is still poorly known. In order to study the features of homocysteinylation of intrinsically unstructured proteins (IUP) bovine caseins were used as a model. αS₁-, β- and κ-caseins, showing ...
DOI:
10.1016/j.bbapap.2011.05.017

33. Allosteric kinetics of the isoform 1 of human glucosamine-6-phosphate deaminase

Author:
Laura I. Álvarez-Añorve; Diego A. Alonzo; Rodrigo Mora-Lugo; Samuel Lara-González; Ismael Bustos-Jaimes; Jacqueline Plumbridge; Mario L. Calcagno
Source:
Biochimica et biophysica acta 2011 v.1814 no.12 pp. 1846-1853
ISSN:
1878-1454
Subject:
Escherichia coli; N-acetylglucosamine; genes; humans; models; mutants
Abstract:
... The human genome contains two genes encoding for two isoforms of the enzyme glucosamine-6-phosphate deaminase (GNPDA, EC 3.5.99.6). Isoform 1 has been purified from several animal sources and the crystallographic structure of the human recombinant enzyme was solved at 1.75Å resolution (PDB ID: 1NE7). In spite of their great structural similarity, human and Escherichia coli GNPDAs show marked diffe ...
DOI:
10.1016/j.bbapap.2011.07.010

34. Alternative photosynthetic electron transport pathways during anaerobiosis in the green alga Chlamydomonas reinhardtii

Author:
Anja Hemschemeier; Thomas Happe
Source:
Biochimica et biophysica acta 2011 v.1807 no.8 pp. 919-926
ISSN:
0005-2728
Subject:
Chlamydomonas reinhardtii; Cyanobacteria; NADP (coenzyme); anaerobic conditions; anaerobiosis; biochemical pathways; chloroplasts; electron transfer; electrons; energy; hydrogen; hydrogen production; oxidants; oxygen; photosynthesis; protons; sulfur
Abstract:
... Oxygenic photosynthesis uses light as energy source to generate an oxidant powerful enough to oxidize water into oxygen, electrons and protons. Upon linear electron transport, electrons extracted from water are used to reduce NADP⁺ to NADPH. The oxygen molecule has been integrated into the cellular metabolism, both as the most efficient electron acceptor during respiratory electron transport and a ...
DOI:
10.1016/j.bbabio.2011.02.010

35. Aminopropyl-functionalized cubic Ia3d mesoporous silica nanoparticle as an efficient support for immobilization of superoxide dismutase

Author:
Mojtaba Falahati; Leila Ma'mani; Ali Akbar Saboury; Abbas Shafiee; Alireza Foroumadi; Ali Reza Badiei
Source:
Biochimica et biophysica acta 2011 v.1814 no.9 pp. 1195-1202
ISSN:
1570-9639
Subject:
Fourier transform infrared spectroscopy; X-ray diffraction; differential scanning calorimetry; fluorescence; immobilized enzymes; melting; nanoparticles; nitrogen; nonionic surfactants; silica; superoxide dismutase; temperature
Abstract:
... In this research, the immobilization of superoxide dismutase (SOD) onto aminopropyl-functionalized KIT-6 [n-PrNH₂-KIT-6] was investigated. This organo-functionalized mesoporous silica nanoparticle was prepared using a non-ionic surfactant and was fully characterized by XRD, nitrogen adsorption–desorption isotherm assay, IR and TGA techniques. An activity assay demonstrated that the immobilized SOD ...
DOI:
10.1016/j.bbapap.2011.04.005

36. Amphotericin B induces trehalose synthesis and simultaneously activates an antioxidant enzymatic response in Candida albicans

Author:
Pilar González-Párraga; Ruth Sánchez-Fresneda; Óscar Zaragoza; Juan-Carlos Argüelles
Source:
Biochimica et biophysica acta 2011 v.1810 no.8 pp. 777-783
ISSN:
0304-4165
Subject:
Candida albicans; amphotericin B; antioxidants; catalase; cell viability; enzyme activity; genes; glutathione-disulfide reductase; messenger RNA; metabolism; mutants; oxidative stress; proteins; reverse transcriptase polymerase chain reaction; superoxide dismutase; trehalase; trehalose
Abstract:
... BACKGROUND: Enzymes involved in trehalose metabolism have been proposed as potential targets for new antifungals. To analyse this proposal, the susceptibility to Amphotericin B (AmB) of the C. albicans trehalose-deficient mutant tps1Δ/tps1Δ, was examined. METHODS: Determination of endogenous trehalose and antioxidant enzymatic activities as well as RT-PCR analysis in cells subjected to AmB treatme ...
DOI:
10.1016/j.bbagen.2011.04.012

37. An NMR study elucidating the binding of Mg(II) and Mn(II) to spinach plastocyanin. Regulation of the binding of plastocyanin to subunit PsaF of photosystem I

Author:
Daniel Farkas; Örjan Hansson
Source:
Biochimica et biophysica acta 2011 v.1807 no.12 pp. 1539-1548
ISSN:
0005-2728
Subject:
binding sites; chloroplasts; dissociation; electrostatic interactions; ions; magnesium; manganese; nuclear magnetic resonance spectroscopy; photosystem I; plastocyanin; spinach
Abstract:
... In this work we address the question whether light-induced changes in the Mg(II) content in the chloroplast lumen can modulate the electron donation to photosystem I, in particular the electrostatic interaction between plastocyanin (Pc) and the photosystem 1 subunit PsaF. For this, we have used 2D NMR spectroscopy to study the binding of Mg(II) ions and the isolated luminal domain of PsaF to ¹⁵N-l ...
DOI:
10.1016/j.bbabio.2011.09.016

38. An analysis of the phosphoproteome of immune cell lines exposed to the immunomodulatory mycotoxin deoxynivalenol

Author:
André Nogueira da Costa; Jeffrey N. Keen; Christopher P. Wild; John B.C. Findlay
Source:
Biochimica et biophysica acta 2011 v.1814 no.7 pp. 850-857
ISSN:
1878-1454
Subject:
biochemical pathways; biomarkers; cell lines; deoxynivalenol; diet; flow cytometry; gels; heat; humans; immunotoxicity; nucleoside-diphosphate kinase; phosphoproteins; phosphorylation; protein synthesis; small cereal grains; two-dimensional gel electrophoresis
Abstract:
... The mycotoxin deoxynivalenol (DON) commonly contaminates cereal grains. It is ubiquitous in the Western European diet, although chronic, low-dose effects in humans are not well described, but immunotoxicity has been reported. In this study, two-dimensional gel electrophoresis was used to identify phosphoproteomic changes in human B (RPMI1788) and T (Jurkat E6.1) lymphocyte cell lines after exposur ...
DOI:
10.1016/j.bbapap.2011.04.001

39. Analogs of 5-(substituted benzylidene)hydantoin as inhibitors of tyrosinase and melanin formation

Author:
Young Mi Ha; Jin-Ah Kim; Yun Jung Park; Daeui Park; Ji Min Kim; Ki Wung Chung; Eun Kyeong Lee; Ji Young Park; Ji Yeon Lee; Hye Jin Lee; Jeong Hyun Yoon; Hyung Ryong Moon; Hae Young Chung
Source:
Biochimica et biophysica acta 2011 v.1810 no.6 pp. 612-619
ISSN:
0304-4165
Subject:
carbon; drugs; mass spectrometry; melanin; mushrooms; nuclear magnetic resonance spectroscopy; spectral analysis; stable isotopes
Abstract:
... BACKGROUND: Many tyrosinase inhibitors find application in cosmetics and pharmaceutical products for the prevention of the overproduction of melanin in the epidermis. A series of 5-(substituted benzylidene)hydantoin derivatives 2a–2k were prepared, and their inhibitory activities toward tyrosinase and melanin formation were evaluated. METHODS: The structures of the compounds were established using ...
DOI:
10.1016/j.bbagen.2011.03.001

40. Analysis of high temperature stress on the dynamics of antenna size and reducing side heterogeneity of Photosystem II in wheat leaves (Triticum aestivum)

Author:
Sonal Mathur; Suleyman I. Allakhverdiev; Anjana Jajoo
Source:
Biochimica et biophysica acta 2011 v.1807 no.1 pp. 22-29
ISSN:
0005-2728
Subject:
Triticum aestivum; chlorophyll; chloroplasts; computer software; crops; fluorescence; heat stress; leaves; light harvesting complex; photosystem II; plant stress; temperature; wheat
Abstract:
... This study demonstrates the effect of high temperature stress on the heterogeneous behavior of PSII in Wheat (Triticum aestivum) leaves. Photosystem II in green plant chloroplasts displays heterogeneity both in the composition of its light harvesting antenna i.e. on the basis of antenna size (α, β and γ centers) and in the ability to reduce the plastoquinone pool i.e. the reducing side of the reac ...
DOI:
10.1016/j.bbabio.2010.09.001

41. Analysis of mammalian fatty acyl-coenzyme A species by mass spectrometry and tandem mass spectrometry

Author:
Christopher A. Haynes
Source:
Biochimica et biophysica acta 2011 v.1811 no.11 pp. 663-668
ISSN:
1388-1981
Subject:
acyl coenzyme A; beta oxidation; biosynthesis; eukaryotic cells; image analysis; liquid chromatography; mammals; mitochondria; peroxisomes; phospholipids; sphingolipids; tandem mass spectrometry; triacylglycerols
Abstract:
... Acyl-CoAs are intermediates of numerous metabolic processes in eukaryotic cells, including beta-oxidation within mitochondria and peroxisomes, and the biosynthesis/remodeling of lipids (e.g. mono-, di-, and triglycerides, phospholipids and sphingolipids). Investigations of lipid metabolism have been advanced by the ability to quantitate acyl-CoA intermediates via liquid chromatography coupled to e ...
DOI:
10.1016/j.bbalip.2011.05.010

42. Analysis of oxysterol metabolomes

Author:
William J. Griffiths; Yuqin Wang
Source:
Biochimica et biophysica acta 2011 v.1811 no.11 pp. 784-799
ISSN:
1388-1981
Subject:
bile acids; blood; brain; cerebrospinal fluid; cholesterol; cholesterol metabolism; gas chromatography-mass spectrometry; image analysis; liquid chromatography; metabolome; phytosterols; reactive oxygen species
Abstract:
... Oxysterols are oxygenated forms of cholesterol. This definition can, however, be expanded to include oxygenated derivatives of plant sterols and also of cholesterol precursors. Oxysterols are formed in the first steps of cholesterol metabolism and also from cholesterol by reactive oxygen species. Oxysterols were once thought of as simple intermediates, or side-products, in the conversion of choles ...
DOI:
10.1016/j.bbalip.2011.05.012

43. Analysis of unsaturated lipids by ozone-induced dissociation

Author:
Simon H.J. Brown; Todd W. Mitchell; Stephen J. Blanksby
Source:
Biochimica et biophysica acta 2011 v.1811 no.11 pp. 807-817
ISSN:
1388-1981
Subject:
chemical structure; dissociation; image analysis; lipids; spectrometers; stereochemistry; tandem mass spectrometry; technology
Abstract:
... Recent developments in analytical technologies have driven significant advances in lipid science. The sensitivity and selectivity of modern mass spectrometers can now provide for the detection and even quantification of many hundreds of lipids in a single analysis. In parallel, increasing evidence from structural biology suggests that a detailed knowledge of lipid molecular structure including car ...
DOI:
10.1016/j.bbalip.2011.04.015

44. Apelin-transgenic mice exhibit a resistance against diet-induced obesity by increasing vascular mass and mitochondrial biogenesis in skeletal muscle

Author:
Toshihiro Yamamoto; Yugo Habata; Yoshio Matsumoto; Yoshitaka Yasuhara; Tadatoshi Hashimoto; Hitomi Hamajyo; Hisashi Anayama; Ryo Fujii; Hiromitsu Fuse; Yasushi Shintani; Masaaki Mori
Source:
Biochimica et biophysica acta 2011 v.1810 no.9 pp. 853-862
ISSN:
0304-4165
Subject:
DNA; NAD (coenzyme); NADH dehydrogenase; adiposity; angiopoietin-1; animal disease models; biogenesis; body temperature; endothelial cells; energy expenditure; food intake; gene expression; gene expression regulation; high fat diet; humans; immunohistochemistry; messenger RNA; mice; muscle fibers; obesity; oxygen consumption; phenotype; receptor protein-tyrosine kinase; skeletal muscle
Abstract:
... BACKGROUND: Apelin is an endogenous ligand for the G-protein-coupled 7-transmembrane receptor, APJ. The administration of apelin-13, a truncated 13-amino acid apelin peptide, in diet-induced obese mice is reported to result in a decrease in adiposity due to the increase of energy expenditure with an increase in the expression of uncoupling proteins. METHODS: We systematically compared the phenotyp ...
DOI:
10.1016/j.bbagen.2011.05.004

45. Apo-10'-lycopenoic acid impacts adipose tissue biology via the retinoic acid receptors

Author:
E. Gouranton; G. Aydemir; E. Reynaud; J. Marcotorchino; C. Malezet; C. Caris-Veyrat; R. Blomhoff; J.F. Landrier; R. Rühl
Source:
Biochimica et biophysica acta 2011 v.1811 no.12 pp. 1105-1114
ISSN:
1388-1981
Subject:
adipocytes; adipogenesis; adipose tissue; anti-inflammatory activity; genes; inflammation; interleukin-1beta; interleukin-6; lycopene; metabolites; microarray technology; models; molecular weight; receptors; retinoic acid; transcriptional activation
Abstract:
... Apo-10'-lycopenoic acid (apo-10-lycac), a metabolite of lycopene, has been shown to possess potent biological activities, notably via the retinoic acid receptors (RAR). In the current study, its impact on adipose tissue and adipocytes was studied. In microarray experiments, the set of genes regulated by apo-10-lycac treatments was compared to the set of genes regulated by all-trans retinoic acid ( ...
DOI:
10.1016/j.bbalip.2011.09.002

46. ApoA-I enhances generation of HDL-like lipoproteins through interaction between ABCA1 and phospholipase Cγ in rat astrocytes

Author:
Jin-ichi Ito; Yuko Nagayasu; Alireza Kheirollah; Sumiko Abe-Dohmae; Shinji Yokoyama
Source:
Biochimica et biophysica acta 2011 v.1811 no.12 pp. 1062-1069
ISSN:
1388-1981
Subject:
ABC transporters; agonists; antibodies; astrocytes; lipoproteins; molecular weight; phosphorylation; polyacrylamide gel electrophoresis; rats; solubilization; tyrosine
Abstract:
... In the previous paper, we reported that apolipoprotein (apo) A-I enhances generation of HDL-like lipoproteins in rat astrocytes to be accompanied with both increase in tyrosine phosphorylation of phospholipase Cγ (PL-Cγ) and PL-Cγ translocation to cytosolic lipid–protein particles (CLPP) fraction. In this paper, we studied the interaction between apoA-I and ATP-binding cassette transporter A1 (ABC ...
DOI:
10.1016/j.bbalip.2011.08.016

47. Application of AFM and optical biosensor for investigation of complexes formed in P450-containing monooxygenase systems

Author:
A.I. Archakov; Yu D. Ivanov
Source:
Biochimica et biophysica acta 2011 v.1814 no.1 pp. 102-110
ISSN:
1878-1454
Subject:
atomic force microscopy; biosensors; cytochrome P-450; enzyme kinetics; ferredoxin-NADP reductase; oxygenases
Abstract:
... Atomic force microscopy (AFM) allows to visualize and count the individual protein molecules and their complexes within multiprotein systems. On the other hand, optical biosensor (OB) provides information on complex formation kinetics as well as complex lifetime (τLT) and affinity. Comparison of complex lifetime τLT with the time required for enzyme's catalytic cycle (τcₐₜ) enables to characterize ...
DOI:
10.1016/j.bbapap.2010.08.013

48. Approaches for targeting mitochondria in cancer therapy

Author:
Gerard G.M. D'Souza; Mayura A Wagle; Vaibhav Saxena; Anee Shah
Source:
Biochimica et biophysica acta 2011 v.1807 no.6 pp. 689-696
ISSN:
0005-2728
Subject:
energy metabolism; human health; medicine; mitochondria; therapeutics
Abstract:
... The recognition of the role that mitochondria play in human health and disease is evidenced by the emergence in recent decades of a whole new field of “Mitochondrial Medicine”. Molecules located on or inside mitochondria are considered prime pharmacological targets and a wide range of efforts are underway to exploit these targets to develop targeted therapies for various diseases including cancer. ...
DOI:
10.1016/j.bbabio.2010.08.008

49. Arginine mediated purification of trehalose-6-phosphate synthase (TPS) from Candida utilis: Its characterization and regulation

Author:
Shinjinee Sengupta; Sagar Lahiri; Shakri Banerjee; Bipasha Bashistha; Anil K. Ghosh
Source:
Biochimica et biophysica acta 2011 v.1810 no.12 pp. 1346-1354
ISSN:
0304-4165
Subject:
Candida utilis; Saccharomyces cerevisiae; adenosine; alpha,alpha-trehalose-phosphate synthase (UDP-forming); anions; arginine; biochemical pathways; chelating agents; chondroitin sulfate; enzyme activity; ethylene glycol tetraacetic acid; fructose 6-phosphate; gel chromatography; glucose 6-phosphate; heparin; magnesium chloride; pH; phosphates; substrate specificity; temperature; trehalose; yeasts
Abstract:
... BACKGROUND: Trehalose is the most important multifunctional, non-reducing disaccharide found in nature. It is synthesized in yeast by an enzyme complex: trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). METHODS: In the present study TPS is purified using a new methodology from Candida utilis cells by inclusion of 100mM l-arginine during cell lysis and in the mobile ...
DOI:
10.1016/j.bbagen.2011.06.025

50. Arthropod CYPomes illustrate the tempo and mode in P450 evolution

Author:
R. Feyereisen
Source:
Biochimica et biophysica acta 2011 v.1814 no.1 pp. 19-28
ISSN:
1878-1454
Subject:
arthropods; genes; insects; models; monophyly; vertebrates
Abstract:
... The great diversity of P450 genes in a variety of organisms is well documented but not well explained. The number of CYP genes in each species is highly variable and this is shown here for arthropod, mainly insect CYPomes. Pairs of recognizable orthologs are but a small portion of the CYPome, but species- or lineage-specific expansions of CYP subfamilies are consistently observed. These “blooms” o ...
DOI:
10.1016/j.bbapap.2010.06.012

51. At the crossroads of steroid hormone biosynthesis: The role, substrate specificity and evolutionary development of CYP17

Author:
Andrei A. Gilep; Tatyana A. Sushko; Sergey A. Usanov
Source:
Biochimica et biophysica acta 2011 v.1814 no.1 pp. 200-209
ISSN:
1878-1454
Subject:
Chordata; biosynthesis; cytochrome P-450; endoplasmic reticulum; mineralocorticoids; pregnenolone; progesterone; steroid hormones; steroids; substrate specificity
Abstract:
... Cytochrome P450s play critical roles in the metabolism of various bioactive compounds. One of the crucial functions of cytochrome P450s in Chordata is in the biosynthesis of steroid hormones. Steroid 17alpha-hydroxylase/17,20-lyase (CYP17) is localized in endoplasmic reticulum membranes of steroidogenic cells. CYP17 catalyzes the 17alpha-hydroxylation reaction of delta4-C₂₁ steroids (progesterone ...
DOI:
10.1016/j.bbapap.2010.06.021

52. AtoSC two-component system is involved in cPHB biosynthesis through fatty acid metabolism in E. coli

Author:
Evaggelos C. Theodorou; Marina C. Theodorou; Dimitrios A. Kyriakidis
Source:
Biochimica et biophysica acta 2011 v.1810 no.5 pp. 561-568
ISSN:
0304-4165
Subject:
Escherichia coli; acrylic acid; beta oxidation; biochemical pathways; biosynthesis; cerulenin; fatty acid metabolism; fatty acids; gene expression; operon; plasmids; spermidine
Abstract:
... BACKGROUND: We have shown previously that AtoSC two-component system regulates the biosynthesis of E. coli cPHB [complexed poly-(R)-3-hydroxybutyrate]. METHODS: The AtoSC involvement on fatty acids metabolism, towards cPHB synthesis, was studied using cPHB determination, gene expression, and fatty acid metabolic pathways inhibitors. RESULTS: Deletion of the atoDAEB operon from the E. coli genome r ...
DOI:
10.1016/j.bbagen.2011.01.014

53. Bacterial growth rate reflects a bottleneck in resource allocation

Author:
A. Goelzer; V. Fromion
Source:
Biochimica et biophysica acta 2011 v.1810 no.10 pp. 978-988
ISSN:
0304-4165
Subject:
bacteria; biochemical pathways; growth performance; mathematical models; microbial growth; resource allocation; ribosomes
Abstract:
... BACKGROUND: Growth rate management in fast-growing bacteria is currently an active research area. In spite of the huge progress made in our understanding of the molecular mechanisms controlling the growth rate, fundamental questions concerning its intrinsic limitations are still relevant today. In parallel, systems biology claims that mathematical models could shed light on these questions. METHOD ...
DOI:
10.1016/j.bbagen.2011.05.014

54. Bacterial sulfite-oxidizing enzymes

Author:
Ulrike Kappler
Source:
Biochimica et biophysica acta 2011 v.1807 no.1 pp. 1-10
ISSN:
0005-2728
Subject:
bacteria; molecular conformation; sulfite oxidase; sulfites
Abstract:
... Enzymes belonging to the Sulfite Oxidase (SO) enzyme family are found in virtually all forms of life, and are especially abundant in prokaryotes as shown by analysis of available genome data. Despite this fact, only a limited number of bacterial SO family enzymes has been characterized in detail to date, and these appear to be involved in very different metabolic processes such as energy generatio ...
DOI:
10.1016/j.bbabio.2010.09.004

55. Binding studies of truncated variants of the Aβ peptide to the V-domain of the RAGE receptor reveal Aβ residues responsible for binding

Author:
Emilia Gospodarska; Anna Kupniewska-Kozak; Grazyna Goch; Michal Dadlez
Source:
Biochimica et biophysica acta 2011 v.1814 no.5 pp. 592-609
ISSN:
1878-1454
Subject:
Alzheimer disease; active transport; animal models; antagonists; blood circulation; brain; cell culture; drugs; fluorescence; mass spectrometry; parenchyma (animal tissue); protein structure; therapeutics; tryptophan
Abstract:
... Alzheimer's disease (AD) symptoms correlate with the concentration of soluble, although not necessarily monomeric forms of Aβ peptide in the brain parenchyma. The RAGE receptor has been implicated as the protein responsible for active transport of Aβ from blood circulation to the brain. In murine models of AD, inhibition of the Aβ:RAGE interaction decreases the levels of Aβ in the brain. Inhibitio ...
DOI:
10.1016/j.bbapap.2011.02.011

56. Bioactive food components, cancer cell growth limitation and reversal of glycolytic metabolism

Author:
Jaap Keijer; Melissa Bekkenkamp-Grovenstein; Dini Venema; Yvonne E.M. Dommels
Source:
Biochimica et biophysica acta 2011 v.1807 no.6 pp. 697-706
ISSN:
0005-2728
Subject:
aerobiosis; apoptosis; bioactive properties; cell growth; energy; enzymes; folic acid; food composition; glucose; glutamine; glycolysis; growth retardation; mitochondria; monitoring; neoplasm cells; omega-3 fatty acids; oxidative phosphorylation; oxygen; polyphenols
Abstract:
... Cancer cells are resistant to apoptosis and show a shift in energy production from mitochondrial oxidative phosphorylation to cytosolic glycolysis. Apoptosis resistance and metabolic reprogramming are linked in many cancer cells and both processes center on mitochondria. Clearly, mutated cancer cells escape surveillance and turn into selfish cells. However, many of the mechanisms that operate cell ...
DOI:
10.1016/j.bbabio.2010.08.007

57. Biochemical and biophysical characterization of succinate: Quinone reductase from Thermus thermophilus

Author:
Olga Kolaj-Robin; Sarah R. O'Kane; Wolfgang Nitschke; Christophe Léger; Frauke Baymann; Tewfik Soulimane
Source:
Biochimica et biophysica acta 2011 v.1807 no.1 pp. 68-79
ISSN:
0005-2728
Subject:
polypeptides; 3-nitropropionic acid; flavoproteins; succinic acid; NAD(P)H dehydrogenase (quinone); crystal structure; heme; polyacrylamide gel electrophoresis; Thermus thermophilus; thermophilic microorganisms; succinate dehydrogenase (quinone); electron paramagnetic resonance spectroscopy; sodium; active sites; enzyme kinetics; structure-activity relationships; temperature; molecular weight
Abstract:
... Enzymes serving as respiratory complex II belong to the succinate:quinone oxidoreductases superfamily that comprises succinate:quinone reductases (SQRs) and quinol:fumarate reductases. The SQR from the extreme thermophile Thermus thermophilus has been isolated, identified and purified to homogeneity. It consists of four polypeptides with apparent molecular masses of 64, 27, 14 and 15kDa, correspon ...
DOI:
10.1016/j.bbabio.2010.10.009

58. Biochemical characterization of purified OmcS, a c-type cytochrome required for insoluble Fe(III) reduction in Geobacter sulfurreducens

Author:
Xinlei Qian; Tünde Mester; Leonor Morgado; Tsutomu Arakawa; Manju L. Sharma; Kengo Inoue; Crisjoe Joseph; Carlos A. Salgueiro; Michael J. Maroney; Derek R. Lovley
Source:
Biochimica et biophysica acta 2011 v.1807 no.4 pp. 404-412
ISSN:
0005-2728
Subject:
Geobacter sulfurreducens; circular dichroism spectroscopy; citrates; electron transfer; electrons; ferric oxide; gene deletion; heme; humic substances; iron; manganese dioxide; melting; molecular weight; oxidation; redox potential; thermal stability
Abstract:
... Previous studies with Geobacter sulfurreducens have demonstrated that OmcS, an abundant c-type cytochrome that is only loosely bound to the outer surface, plays an important role in electron transfer to Fe(III) oxides as well as other extracellular electron acceptors. In order to further investigate the function of OmcS, it was purified from a strain that overproduces the protein. Purified OmcS ha ...
DOI:
10.1016/j.bbabio.2011.01.003

59. Bioconversion of vitamin D to its active form by bacterial or mammalian cytochrome P450

Author:
Toshiyuki Sakaki; Hiroshi Sugimoto; Keiko Hayashi; Kaori Yasuda; Eiji Munetsuna; Masaki Kamakura; Shinichi Ikushiro; Yoshitsugu Shiro
Source:
Biochimica et biophysica acta 2011 v.1814 no.1 pp. 249-256
ISSN:
1878-1454
Subject:
Pseudonocardia autotrophica; Streptomyces griseolus; bacteria; bacterial proteins; biotransformation; crystal structure; cytochrome P-450; enzymes; evolution; fungi; genes; mammals; site-directed mutagenesis; vitamin D
Abstract:
... Bioconversion processes, including specific hydroxylations, promise to be useful for practical applications because chemical syntheses often involve complex procedures. One of the successful applications of P450 reactions is the bioconversion of vitamin D₃ to 1α,25-dihydroxyvitamin D₃. Recently, a cytochrome P450 gene encoding a vitamin D hydroxylase from the CYP107 family was cloned from Pseudono ...
DOI:
10.1016/j.bbapap.2010.07.014

60. Biological targets of isothiocyanates

Author:
Kristin K. Brown; Mark B. Hampton
Source:
Biochimica et biophysica acta 2011 v.1810 no.9 pp. 888-894
ISSN:
0304-4165
Subject:
anti-inflammatory activity; antioxidants; apoptosis; cytokines; drugs; homeostasis; isothiocyanates; methodology; oxidation; phytopharmaceuticals; proteins; site-directed mutagenesis; structure-activity relationships; thiols
Abstract:
... BACKGROUND: Isothiocyanates are phytochemicals with a broad array of effects in biological systems. Bioactivity includes the stimulation of cellular antioxidant systems, induction of apoptosis and interference with cytokine production and activity. Epidemiological evidence and experimental studies indicate that naturally occurring isothiocyanates and synthetic derivatives have anti-cancer and anti ...
DOI:
10.1016/j.bbagen.2011.06.004

61. Biophysical characterization of α-amylase inhibitor Parvulustat (Z-2685) and comparison with Tendamistat (HOE-467)

Author:
Alma Sokočević; Sigeng Han; Joachim W. Engels
Source:
Biochimica et biophysica acta 2011 v.1814 no.10 pp. 1383-1393
ISSN:
1878-1454
Subject:
alpha-amylase; fluorescence; nuclear magnetic resonance spectroscopy; pH; proteins; temperature; thermodynamics
Abstract:
... Parvulustat is a small, highly active proteinaceous α-amylase inhibitor whose high-resolution NMR structure was recently solved in Frankfurt. Here, we present its biochemical and biophysical characterization. Several spectroscopic methods such as UV, fluorescence and CD were utilized to extract conformational changes upon modification of pH, temperature and chemical denaturant. Parvulustat reveale ...
DOI:
10.1016/j.bbapap.2011.07.009

62. Biosurfactant mannosyl-erythritol lipid inhibits secretion of inflammatory mediators from RBL-2H3 cells

Author:
Yosuke Morita; Satoshi Tadokoro; Masao Sasai; Dai Kitamoto; Naohide Hirashima
Source:
Biochimica et biophysica acta 2011 v.1810 no.12 pp. 1302-1308
ISSN:
0304-4165
Subject:
Western blotting; antigens; apoptosis; bioactive properties; biosurfactants; calcium; cell differentiation; dose response; drugs; enzyme-linked immunosorbent assay; exocytosis; fluorescent dyes; glycolipids; mast cells; mechanism of action; membrane fusion; microorganisms; mitogen-activated protein kinase; necrosis; phosphorylation; proteins; secretion; signal transduction; tumor necrosis factor-alpha
Abstract:
... BACKGROUND: Biosurfactant mannosyl-erythritol lipids (MELs) are glycolipids produced by microbes that have various biological activities. It has been reported that MELs exhibit excellent surface-activity and also various bioactivities, such as induction of cell differentiation and apoptosis. However, little is known about their action related to drug discovery or drug seeds. METHODS: We investigat ...
DOI:
10.1016/j.bbagen.2011.07.002

63. Bis (monoacylglycero) phosphate interfacial properties and lipolysis by pancreatic lipase-related protein 2, an enzyme present in THP-1 human monocytes

Author:
Michel Record; Sawsan Amara; Caroline Subra; Guowei Jiang; Glenn D. Prestwich; Francine Ferrato; Frédéric Carrière
Source:
Biochimica et biophysica acta 2011 v.1811 no.7-8 pp. 419-430
ISSN:
1388-1981
Subject:
enzyme activity; humans; lipolysis; monocytes; pH; phosphates; phospholipase A1; phospholipids; physical properties; surface area
Abstract:
... The interfacial physical properties of bis(monoacylglycero)phosphate (BMP) and its derivatives with three oleoyl chains (hemi-BDP) and four oleoyl chains (bis(diacylglycero)phosphate, BDP) were investigated using Langmuir monomolecular films. The mean molecular area of BMP at the collapse surface pressure (45mN m⁻¹) was similar to those measured with other phospholipids bearing two acyl chains (66 ...
DOI:
10.1016/j.bbalip.2011.04.008

64. Bis-THF motif of acetogenin binds to the third matrix-side loop of ND1 subunit in mitochondrial NADH-ubiquinone oxidoreductase

Author:
Sayo Nakanishi; Masato Abe; Shuhei Yamamoto; Masatoshi Murai; Hideto Miyoshi
Source:
Biochimica et biophysica acta 2011 v.1807 no.9 pp. 1170-1176
ISSN:
0005-2728
Subject:
NADH dehydrogenase (ubiquinone); acetogenins; cattle; crosslinking; heart; photoaffinity labeling; proteinases
Abstract:
... Natural acetogenins are among the most potent inhibitors of bovine heart mitochondrial NADH-ubiquinone oxidoreductase (complex I). Our photoaffinity labeling study suggested that the hydroxylated bis-THF ring moiety of acetogenins binds at “site A” in the third matrix-side loop connecting the fifth and sixth transmembrane helices in the ND1 subunit [Kakutani et al. (2010) Biochemistry 49, 4794–480 ...
DOI:
10.1016/j.bbabio.2011.05.012

65. Brain Specific Kinase-1 BRSK1/SAD-B associates with lipid rafts: modulation of kinase activity by lipid environment

Author:
Arantza Rodríguez-Asiain; Gerard Ruiz-Babot; Walter Romero; Roger Cubí; Tatiana Erazo; Ricardo M. Biondi; Jose R. Bayascas; Jose Aguilera; Nestor Gómez; Carles Gil; Enrique Claro; Jose M. Lizcano
Source:
Biochimica et biophysica acta 2011 v.1811 no.12 pp. 1124-1135
ISSN:
1388-1981
Subject:
brain; cholesterol; humans; phosphatidylcholines; phosphorylation; phosphotransferases (kinases); proteins; sphingomyelins; synaptic transmission; threonine
Abstract:
... Brain specific kinases 1 and 2 (BRSK1/2, also named SAD kinases) are serine–threonine kinases specifically expressed in the brain, and activated by LKB1-mediated phosphorylation of a threonine residue at their T-loop (Thr189/174 in human BRSK1/2). BRSKs are crucial for establishing neuronal polarity, and BRSK1 has also been shown to regulate neurotransmitter release presynaptically. How BRSK1 exer ...
DOI:
10.1016/j.bbalip.2011.10.004

66. Brazilian propolis-derived components inhibit TNF-α-mediated downregulation of adiponectin expression via different mechanisms in 3T3-L1 adipocytes

Author:
Rie Ikeda; Masayoshi Yanagisawa; Nobuyuki Takahashi; Teruo Kawada; Shigenori Kumazawa; Noriyuki Yamaotsu; Izumi Nakagome; Shuichi Hirono; Takanori Tsuda
Source:
Biochimica et biophysica acta 2011 v.1810 no.7 pp. 695-703
ISSN:
0304-4165
Subject:
adipocytes; adiponectin; agonists; fatty acid-binding proteins; insulin resistance; mitogen-activated protein kinase; molecular models; necrosis; propolis; transcription (genetics); transcriptional activation; tumor necrosis factor-alpha
Abstract:
... BACKGROUND: Previous reports suggest that Brazilian propolis has multiple biological functions and may help to restore adiponectin expression and insulin sensitivity. However, little is known about the molecular mechanisms by which these compounds inhibit the downregulation of adiponectin. METHODS: The effect of various Brazilian propolis-derived components on inhibition of tumor necrosis factor-α ...
DOI:
10.1016/j.bbagen.2011.04.007

67. Breakthrough in P450 bioelectrochemistry and future perspectives

Author:
Sheila J. Sadeghi; Andrea Fantuzzi; Gianfranco Gilardi
Source:
Biochimica et biophysica acta 2011 v.1814 no.1 pp. 237-248
ISSN:
1878-1454
Subject:
bioelectrochemistry; cytochrome P-450; electrodes; electron transfer; enzymes
Abstract:
... Improving the electrochemical performance of cytochrome P450 enzymes is highly desirable due to their versatility in the recognition of different biological and xenobiotic compounds. The task poses an exciting challenge because it leads not only to the acquisition of fundamental knowledge on the redox properties of these enzymes, but it also opens opportunities for technological and commercial app ...
DOI:
10.1016/j.bbapap.2010.07.010

68. Broadband dielectric spectroscopy on human blood

Author:
M. Wolf; R. Gulich; P. Lunkenheimer; A. Loidl
Source:
Biochimica et biophysica acta 2011 v.1810 no.8 pp. 727-740
ISSN:
0304-4165
Subject:
absorption; blood plasma; cell membranes; dielectric properties; dielectric spectroscopy; electromagnetic radiation; hematocrit; humans; prediction; temperature
Abstract:
... BACKGROUND: Dielectric spectra of human blood reveal a rich variety of dynamic processes. Achieving a better characterization and understanding of these processes not only is of academic interest but also of high relevance for medical applications as, e.g., the determination of absorption rates of electromagnetic radiation by the human body. METHODS: The dielectric properties of human blood are st ...
DOI:
10.1016/j.bbagen.2011.05.012

69. CTP:phosphocholine cytidylyltransferase α (CCTα) and lamins alter nuclear membrane structure without affecting phosphatidylcholine synthesis

Author:
Karsten Gehrig; Neale D. Ridgway
Source:
Biochimica et biophysica acta 2011 v.1811 no.6 pp. 377-385
ISSN:
1388-1981
Subject:
RNA interference; choline; cytoplasm; deformation; ethanolamine; fibroblasts; metabolism; mutants; nuclear membrane; phosphatidylcholines; reticulum
Abstract:
... CTP:phosphocholine cytidylyltransferase α (CCTα) is a nuclear enzyme that catalyzes the rate-limiting step in the CDP–choline pathway for phosphatidylcholine (PC) synthesis. Lipid activation of CCTα results in its translocation to the nuclear envelope and expansion of an intranuclear membrane network termed the nucleoplasmic reticulum (NR) by a mechanism involving membrane deformation. Nuclear lam ...
DOI:
10.1016/j.bbalip.2011.04.001

70. Carbamoyl-PROXYL-enhanced MRI detects very small disruptions in brain vascular permeability induced by dietary cholesterol

Author:
Atsuyuki Tomizawa; Itsuko Ishii; Zhivko Zhelev; Ichio Aoki; Sayaka Shibata; Mitsukazu Kitada; Rumiana Bakalova
Source:
Biochimica et biophysica acta 2011 v.1810 no.12 pp. 1309-1316
ISSN:
0304-4165
Subject:
animal models; blood-brain barrier; brain; cholesterol; diet; half life; hypercholesterolemia; magnetic resonance imaging; metalloproteinases; mice; neutrophils; permeability
Abstract:
... BACKGROUND: Gd-DTPA-enhanced magnetic resonance imaging (MRI) is a conventional method for non-invasive investigation of blood-brain-barrier (BBB) permeability in animal models. It allows the visualization of serious injury to the BBB. We developed a novel approach for detecting very small disruptions in BBB permeability induced by dietary cholesterol by using carbamoyl-PROXYL (CMP) as an MRI cont ...
DOI:
10.1016/j.bbagen.2011.06.011

71. Carbon metabolism and the sign of control coefficients in metabolic adaptations underlying K-ras transformation

Author:
Pedro de Atauri; Adrian Benito; Pedro Vizán; Miriam Zanuy; Ramón Mangues; Silvia Marín; Marta Cascante
Source:
Biochimica et biophysica acta 2011 v.1807 no.6 pp. 746-754
ISSN:
0005-2728
Subject:
NADP-glucose-6-phosphate dehydrogenase; energy metabolism; transketolase; lactate dehydrogenase; pyruvate kinase; carbon; enzyme kinetics; glycolysis; case studies; enzyme activity; metabolites; prediction
Abstract:
... Metabolic adaptations are associated with changes in enzyme activities. These adaptations are characterized by patterns of positive and negative changes in metabolic fluxes and concentrations of intermediate metabolites. Knowledge of the mechanism and parameters governing enzyme kinetics is rarely available. However, the signs—increases or decreases—of many of these changes can be predicted using ...
DOI:
10.1016/j.bbabio.2010.11.015

72. Cardiac mitochondria in heart failure: Normal cardiolipin profile and increased threonine phosphorylation of complex IV

Author:
Mariana Rosca; Paul Minkler; Charles L. Hoppel
Source:
Biochimica et biophysica acta 2011 v.1807 no.11 pp. 1373-1382
ISSN:
0005-2728
Subject:
cardiomyocytes; cyclic AMP; glutamic acid; heart failure; mitochondria; mitochondrial membrane; oxidation; oxidative phosphorylation; phospholipids; protein phosphorylation; proteins; threonine
Abstract:
... Mitochondrial dysfunction is a major contributor in heart failure (HF). We investigated whether the decrease in respirasome organization reported by us previously in cardiac mitochondria in HF is due to changes in the phospholipids of the mitochondrial inner membrane or modifications of the subunits of the electron transport chain (ETC) complexes. The contents of the main phospholipid species, inc ...
DOI:
10.1016/j.bbabio.2011.02.003

73. Carotenoids are essential for normal levels of dimerisation of the RC–LH1–PufX core complex of Rhodobacter sphaeroides: Characterisation of R-26 as a crtB (phytoene synthase) mutant

Author:
Irene W. Ng; Peter G. Adams; David J. Mothersole; Cvetelin Vasilev; Elizabeth C. Martin; Helen P. Lang; Jaimey D. Tucker; C. Neil Hunter
Source:
Biochimica et biophysica acta 2011 v.1807 no.9 pp. 1056-1063
ISSN:
0005-2728
Subject:
Rhodobacter sphaeroides; atomic force microscopy; carotenoids; electron microscopy; energy transfer; fractionation; genes; mutants; mutation; photosynthesis; phytoene synthase; polypeptides
Abstract:
... Carotenoids play important roles in photosynthesis where they are involved in light-harvesting, in photo-protection and in the assembly and structural stability of light-harvesting and reaction centre complexes. In order to examine the effects of carotenoids on the oligomeric state of the reaction centre–light-harvesting 1 -PufX (RC–LH1–PufX) core complex of Rhodobacter sphaeroides two carotenoid- ...
DOI:
10.1016/j.bbabio.2011.05.020

74. Catalytic intermediates of cytochrome bd terminal oxidase at steady-state: Ferryl and oxy-ferrous species dominate

Author:
Vitaliy B. Borisov; Elena Forte; Paolo Sarti; Alessandro Giuffrè
Source:
Biochimica et biophysica acta 2011 v.1807 no.5 pp. 503-509
ISSN:
0005-2728
Subject:
Escherichia coli; absorption; cytochrome-c oxidase; dithiothreitol; heme; mammals; models; oxidation; oxygen; proton-motive force; spectroscopy; ubiquinones
Abstract:
... The cytochrome bd ubiquinol oxidase from Escherichia coli couples the exergonic two-electron oxidation of ubiquinol and four-electron reduction of O₂ to 2H₂O to proton motive force generation by transmembrane charge separation. The oxidase contains two b-type hemes (b₅₅₈ and b₅₉₅) and one heme d, where O₂ is captured and converted to water through sequential formation of a few intermediates. The s ...
DOI:
10.1016/j.bbabio.2011.02.007

75. Catalytic oxidation of o-aminophenols and aromatic amines by mushroom tyrosinase

Author:
Jose Luis Muñoz-Muñoz; Francisco Garcia-Molina; Pedro Antonio Garcia-Ruiz; Ramon Varon; Jose Tudela; Jose N. Rodriguez-Lopez; Francisco Garcia-Canovas
Source:
Biochimica et biophysica acta 2011 v.1814 no.12 pp. 1974-1983
ISSN:
1878-1454
Subject:
aromatic amines; monoamines; active sites; oxidation; mushrooms; histidine
Abstract:
... The kinetics of tyrosinase acting on o-aminophenols and aromatic amines as substrates was studied. The catalytic constants of aromatic monoamines and o-diamines were both low, these results are consistent with our previous mechanism in which the slow step is the transfer of a proton by a hydroxyl to the peroxide in oxy-tyrosinase (Fenoll et al., Biochem. J. 380 (2004) 643–650). In the case of o-am ...
DOI:
10.1016/j.bbapap.2011.07.015

76. Caudal-related homeodomain proteins CDX1/2 bind to DNA replication-related element binding factor

Author:
So Young Park; Mi Suk Jeong; Mi-Ae Yoo; Se Bok Jang
Source:
Biochimica et biophysica acta 2011 v.1814 no.12 pp. 1891-1899
ISSN:
1878-1454
Subject:
DNA; DNA-binding domains; Drosophila; Escherichia coli; amino acids; chromatography; computer software; fluorescence; genes; homeodomain proteins; intestinal mucosa; models; mutants; polypeptides; spectroscopy; surface plasmon resonance; transcription factors
Abstract:
... In the intestinal epithelium, the CDX1 and CDX2 homeodomain genes play proliferative and tumor suppressor roles, respectively. The transcription factor DNA replication-related element binding factor (DREF), is an 80kDa polypeptide homodimer that plays an important role in regulating cell proliferation-related genes. Homeodomain genes encode DNA-binding proteins that play crucial roles during devel ...
DOI:
10.1016/j.bbapap.2011.07.021

77. Cell cycle-dependent conjugation of endogenous BRCA1 protein with SUMO-2/3

Author:
Aurélie Vialter; Anne Vincent; Aïcha Demidem; Daniel Morvan; Georges Stepien; Nicole Dalla Venezia; Pascale G. Rio
Source:
Biochimica et biophysica acta 2011 v.1810 no.4 pp. 432-438
ISSN:
0304-4165
Subject:
DNA repair; T-lymphocytes; Western blotting; animal ovaries; breast neoplasms; carcinogenesis; cell cycle; genes; neoplasm cells; ovarian neoplasms; oxidative stress; post-translational modification; precipitin tests; sequence analysis; signal transduction; stress response; transcription (genetics); tumor suppressor proteins
Abstract:
... BACKGROUND: BRCA1, the main breast and ovarian cancer susceptibility gene, has a key role in maintenance of genome stability, cell cycle and transcription regulation. Interestingly, some of the numerous proteins which interact with BRCA1 protein undergo conjugation with small ubiquitin-like modifiers (SUMO). This post-translational modification is related to transcription, DNA repair, nuclear tran ...
DOI:
10.1016/j.bbagen.2010.12.001

78. Cells as semantic systems

Author:
Dennis Görlich; Stefan Artmann; Peter Dittrich
Source:
Biochimica et biophysica acta 2011 v.1810 no.10 pp. 914-923
ISSN:
0304-4165
Subject:
entropy; microorganisms; models
Abstract:
... BACKGROUND: We consider cells as biological systems that process information by means of molecular codes. Many studies analyze cellular information processing exclusively in syntactic terms (e.g., by measuring Shannon entropy of sets of macromolecules), and abstract completely from semantic aspects that are related to the meaning of molecular information. METHODS: This mini-review focusses on sema ...
DOI:
10.1016/j.bbagen.2011.04.004

79. ChREBP expression in the liver, adipose tissue and differentiated preadipocytes in human obesity

Author:
Carmen Hurtado del Pozo; Gregorio Vesperinas-García; Miguel-Ángel Rubio; Ramón Corripio-Sánchez; Antonio J. Torres-García; Maria-Jesus Obregon; Rosa María Calvo
Source:
Biochimica et biophysica acta 2011 v.1811 no.12 pp. 1194-1200
ISSN:
1388-1981
Subject:
Western blotting; adipocytes; adipogenesis; adipose tissue; biopsy; humans; lipogenesis; liver; messenger RNA; obesity; patients; protein synthesis; reverse transcriptase polymerase chain reaction; transcription factors
Abstract:
... ChREBP is an essential transcription factor for lipogenesis. Its physiological role in adipose tissue has been studied only to a small extent and the control of its expression remains unknown in human adipocytes. We have studied ChREBP mRNA and protein expression levels in the liver and the omental (OM) and subcutaneous (SC) adipose tissues from obese and lean subjects, as well as in human differe ...
DOI:
10.1016/j.bbalip.2011.07.016

80. Chaperonins induce an amyloid-like transformation of ovine prion protein: The fundamental difference in action between eukaryotic TRiC and bacterial GroEL

Author:
Georgy G. Kiselev; Irina N. Naletova; Evgeny V. Sheval; Yulia Y. Stroylova; Elena V. Schmalhausen; Thomas Haertlé; Vladimir I. Muronetz
Source:
Biochimica et biophysica acta 2011 v.1814 no.12 pp. 1730-1738
ISSN:
1878-1454
Subject:
amyloid; chaperonins; electron microscopy; intestinal microorganisms; light scattering; neurodegenerative diseases; particles; pathogenesis; prions; sheep
Abstract:
... Molecular chaperones have been shown to be involved in the processes taking place during the pathogenesis of various amyloid neurodegenerative diseases. However, contradictory literature reports suggest that different molecular chaperones can either stimulate or prevent the formation of amyloid structures from distinct amyloidogenic proteins. In the present work, we concentrated on the effects cau ...
DOI:
10.1016/j.bbapap.2011.08.006

81. Characterization of JBURE-IIb isoform of Canavalia ensiformis (L.) DC urease

Author:
Fernanda Mulinari; Arlete Beatriz Becker-Ritt; Diogo Ribeiro Demartini; Rodrigo Ligabue-Braun; Fernanda Stanisçuaski; Hugo Verli; Rodrigo R. Fragoso; Evelyn Koeche Schroeder; Célia Regina Carlini; Maria Fátima Grossi-de-Sá
Source:
Biochimica et biophysica acta 2011 v.1814 no.12 pp. 1758-1768
ISSN:
1878-1454
Subject:
Canavalia ensiformis; Escherichia coli; Malpighian tubules; Rhodnius prolixus; active sites; ammonia; bacteria; bicarbonates; complementary DNA; diuresis; genes; hydrolysis; mass spectrometry; models; nickel; peptides; plant pathogenic fungi; protective effect; recombinant proteins; seed extracts; urea; urease
Abstract:
... Ureases, nickel-dependent enzymes that catalyze the hydrolysis of urea into ammonia and bicarbonate, are widespread in plants, bacteria, and fungi. Previously, we cloned a cDNA encoding a Canavalia ensiformis urease isoform named JBURE-II, corresponding to a putative smaller urease protein (78kDa) when compared to other plant ureases. Aiming to produce the recombinant protein, we obtained jbure-II ...
DOI:
10.1016/j.bbapap.2011.07.022

82. Characterization of a novel posttranslational modification in neuronal nitric oxide synthase by small ubiquitin-related modifier-1

Author:
Masatomo Watanabe; Kouichi Itoh
Source:
Biochimica et biophysica acta 2011 v.1814 no.7 pp. 900-907
ISSN:
1878-1454
Subject:
bioinformatics; mass spectrometry; neuronal nitric oxide synthase; nitric oxide; phosphorylation; recombinant proteins; ubiquitination
Abstract:
... The multifaceted functions of nitric oxide (NO) in the CNS are defined by the activity of neuronal NO synathase (nNOS). The activities of nNOS are modulated by posttranslational modifications, such as phosphorylation and ubiquitination, but whether it is modified by small ubiquitin-related modifier (SUMO) remains unknown. The aim of this study was to elucidate whether nNOS is posttranslationally m ...
DOI:
10.1016/j.bbapap.2011.04.006

83. Characterization of calcium ion sensitive region for β-Mannanase from Streptomyces thermolilacinus

Author:
Yuya Kumagai; Hirokazu Usuki; Yukihiro Yamamoto; Akihiro Yamasato; Jiro Arima; Takafumi Mukaihara; Tadashi Hatanaka
Source:
Biochimica et biophysica acta 2011 v.1814 no.9 pp. 1127-1133
ISSN:
1878-1454
Subject:
Escherichia coli; Streptomyces; Thermobifida fusca; calcium; chimerism; enzymes; genes; hydrolysis; industrial applications; metal ions; temperature; thermal stability
Abstract:
... Despite the widespread industrial applications of β-mannanase, the relations between the enzymatic properties and metal ions remain poorly understood. To elucidate the effects of metal ions on β-mannanase, thermal stability and hydrolysis activity were characterized. The stman and tfman genes encoding β-mannanase (EC.3.2.1.78) from Streptomyces thermolilacinus NBRC14274 and Thermobifida fusca NBRC ...
DOI:
10.1016/j.bbapap.2011.04.017

84. Characterization of low-energy excited states in the native state ensemble of non-myristoylated and myristoylated neuronal calcium sensor-1

Author:
Kousik Chandra; Yogendra Sharma; K.V.R. Chary
Source:
Biochimica et biophysica acta 2011 v.1814 no.2 pp. 334-344
ISSN:
1878-1454
Subject:
calcium; energy; polypeptides; proteins; temperature
Abstract:
... Information on the low-energy excited states of a given protein is important as this controls the structural adaptability and various biological functions of proteins such as co-operativity, response towards various external perturbations. In this article, we characterized individual residues in both non-myristoylated (non-myr) and myristoylated (myr) neuronal calcium sensor-1 (NCS-1) that access ...
DOI:
10.1016/j.bbapap.2010.10.007

85. Characterization of monomeric dihydrodipicolinate synthase variant reveals the importance of substrate binding in optimizing oligomerization

Author:
F. Grant Pearce; Renwick C.J. Dobson; Geoffrey B. Jameson; Matthew A. Perugini; Juliet A. Gerrard
Source:
Biochimica et biophysica acta 2011 v.1814 no.12 pp. 1900-1909
ISSN:
1570-9639
Subject:
Escherichia coli; Thermotoga maritima; X-radiation; X-ray diffraction; catalytic activity; enzymes; gel chromatography; mutation; pyruvic acid; thermal stability; ultracentrifugation
Abstract:
... To gain insights into the role of quaternary structure in the TIM-barrel family of enzymes, we introduced mutations to the DHDPS enzyme of Thermotoga maritima, which we have previously shown to be a stable tetramer in solution. These mutations were aimed at reducing the number of salt bridges at one of the two tetramerization interface of the enzyme, which contains many more interactions than the ...
DOI:
10.1016/j.bbapap.2011.07.016

86. Characterization of photosystem II in transgenic tobacco plants with decreased iron superoxide dismutase

Author:
Yan Zhang; Shunhua Ding; Qingtao Lu; Zhipan Yang; Xiaogang Wen; Lixin Zhang; Congming Lu
Source:
Biochimica et biophysica acta 2011 v.1807 no.4 pp. 391-403
ISSN:
0005-2728
Subject:
D1 protein; Nicotiana tabacum; RNA interference; electron transfer; fluorescence; gels; genes; photosystem II; protein degradation; redox potential; spectroscopy; superoxide anion; superoxide dismutase; thermoluminescence; tobacco; transgenic plants
Abstract:
... Iron superoxide dismutases (FeSODs) play an important role in preventing the oxidative damage associated with photosynthesis. To investigate the mechanisms of FeSOD in protection against photooxidative stress, we obtained transgenic tobacco (Nicotiana tabacum) plants with severely decreased FeSOD by using a gene encoding tobacco chloroplastic FeSOD for the RNAi construct. Transgenic plants were hi ...
DOI:
10.1016/j.bbabio.2011.01.006

87. Characterization of the L683P mutation of SLC26A9 in Xenopus oocytes

Author:
Martine Avella; Franck Borgese; Jordi Ehrenfeld
Source:
Biochimica et biophysica acta 2011 v.1810 no.6 pp. 277-283
ISSN:
0304-4165
Subject:
Xenopus; biotinylation; chlorides; leucine; monitoring; mutants; mutation; oocytes; patch-clamp technique; plasma membrane; surface proteins
Abstract:
... In the present study, we characterized a STAS-domain amino acid mutation of SLC26A9 having a significant impact on ion transport. We focused on the sole conserved L- leucine residue of the STAS domain identified among SLC26 members. We therefore characterized the L683P mutation of SLC26A9 in Xenopus oocytes by monitoring the protein functional expression (two-electrode technique for voltage-clamp ...
DOI:
10.1016/j.bbagen.2011.03.012

88. Characterization of the aldol condensation activity of the trans-o-hydroxybenzylidenepyruvate hydratase-aldolase (tHBP-HA) cloned from Pseudomonas fluorescens N3

Author:
Silvia Ferrara; Erika Mapelli; Guido Sello; Patrizia Di Gennaro
Source:
Biochimica et biophysica acta 2011 v.1814 no.5 pp. 622-629
ISSN:
1878-1454
Subject:
Escherichia coli; Pseudomonas fluorescens; affinity chromatography; fructose-bisphosphate aldolase; genes; hydroxybenzaldehyde; naphthalene; pH; pyruvic acid; recombinant proteins; temperature
Abstract:
... The gene encoding trans-o-hydroxybenzylidenepyruvate hydratase-aldolase (tHBP-HA) was isolated from Pseudomonas fluorescens N3, an environmental strain able to degrade naphthalene. This enzyme is an aldolase of class I that reversibly catalyzes the transformation of the trans-o-hydroxybenzylidenepyruvate (t-HBP), releasing pyruvate and salicylaldehyde. The enzyme was expressed in Escherichia coli ...
DOI:
10.1016/j.bbapap.2011.03.013

89. Characterization of the single-stranded DNA binding protein pVⱽᴳᴶΦ of VGJΦ phage from Vibrio cholerae

Author:
Alina Falero; Andy Caballero; Sonia Trigueros; Celso Pérez; Javier Campos; Karen Marrero; Rafael Fando
Source:
Biochimica et biophysica acta 2011 v.1814 no.9 pp. 1107-1112
ISSN:
1878-1454
Subject:
DNA-binding proteins; Vibrio cholerae; bacteriophages; bioactive properties; bioinformatics; frozen storage; gel chromatography; gel electrophoresis; isoelectric point; molecular weight; pH; single-stranded DNA; sodium chloride; sodium phosphate; temperature
Abstract:
... pVⱽᴳᴶΦ, a single-stranded DNA binding protein of the vibriophage VGJΦ was subject to biochemical analysis. Here, we show that this protein has a general affinity for single-stranded DNA (ssDNA) as documented by Electrophoretic Mobility Shift Assay (EMSA). The apparent molecular weight of the monomer is about 12.7kDa as measured by HPLC–SEC. Moreover, isoelectrofocusing showed an isoelectric point ...
DOI:
10.1016/j.bbapap.2011.04.013

90. Characterization of two novel nodule-enhanced α-type carbonic anhydrases from Lotus japonicus

Author:
Daniela Tsikou; Catalina Stedel; Evangelia D. Kouri; Michael K. Udvardi; Trevor L. Wang; Panagiotis Katinakis; Nikolaos E. Labrou; Emmanouil Flemetakis
Source:
Biochimica et biophysica acta 2011 v.1814 no.4 pp. 496-504
ISSN:
1878-1454
Subject:
Escherichia coli; Lotus corniculatus var. japonicus; Mesorhizobium loti; carbon dioxide; carbonate dehydratase; clones; complementary DNA; gene expression; gene expression regulation; genes; heterologous gene expression; legumes; metabolism; mutants; nitrogen fixation; polypeptides; proteins; vascular bundles
Abstract:
... Two cDNA clones coding for α-type carbonic anhydrases (CA; EC 4.2.1.1) in the nitrogen-fixing nodules of the model legume Lotus japonicus were identified. Functionality of the full-length proteins was confirmed by heterologous expression in Escherichia coli and purification of the encoded polypeptides. The developmental expression pattern of LjCAA1 and LjCAA2 revealed that both genes code for nodu ...
DOI:
10.1016/j.bbapap.2011.01.007

91. Charged residues on a flap-loop structure of Lactococcus lactis prolidase play critical roles in allosteric behavior and substrate inhibition

Author:
Jian An Chen; Takuji Tanaka
Source:
Biochimica et biophysica acta 2011 v.1814 no.12 pp. 1677-1685
ISSN:
1878-1454
Subject:
Lactococcus lactis; active sites; aspartic acid; catalytic activity; enzymatic reactions; molecular models; mutants; mutation; proline dipeptidase; substrate specificity
Abstract:
... Allosteric behavior and substrate inhibition are unique characteristics of Lactococcus lactis prolidase. We hypothesized that charged residues (Asp36, His38, Glu39, and Arg40), present on one loop essential for catalysis, interact with residues in or near the active site to impart these unique characteristics. Asp36 has a predominant role in the allosteric behavior, as demonstrated through the non ...
DOI:
10.1016/j.bbapap.2011.08.009

92. Chemiosmotic coupling in oxidative and photosynthetic phosphorylation

Author:
Peter Mitchell
Source:
Biochimica et biophysica acta 2011 v.1807 no.12 pp. 1507-1538
ISSN:
0005-2728
Subject:
energy metabolism; phosphorylation; proton-motive force; scientists; England
Abstract:
... 50years ago Peter Mitchell proposed the chemiosmotic hypothesis for which he was awarded the Nobel Prize for Chemistry in 1978. His comprehensive review on chemiosmotic coupling known as the first “Grey Book”, has been reprinted here with permission, to offer an electronic record and easy access to this important contribution to the biochemical literature. This remarkable account of Peter Mitchell ...
DOI:
10.1016/j.bbabio.2011.09.018

93. Chip-based nanoelectrospray mass spectrometry of brain gangliosides

Author:
Corina Flangea; Alina Serb; Eugen Sisu; Alina D. Zamfir
Source:
Biochimica et biophysica acta 2011 v.1811 no.9 pp. 513-535
ISSN:
1388-1981
Subject:
biomarkers; brain; electrospray ionization mass spectrometry; gangliosides; ionization; neoplasms; neurodegenerative diseases; polymers
Abstract:
... In the past few years, a considerable effort was invested in interfacing mass spectrometry (MS) to microfluidics-based systems for electrospray ionization (ESI). Since its first introduction in biological mass spectrometry, chip-based ESI demonstrated a high potential to discover novel structures of biomarker value. Therefore, recently, microfluidics for electrospray in conjunction with advanced M ...
DOI:
10.1016/j.bbalip.2011.06.008

94. Chlorophyll breakdown in higher plants

Author:
Stefan Hörtensteiner; Bernhard Kräutler
Source:
Biochimica et biophysica acta 2011 v.1807 no.8 pp. 977-988
ISSN:
0005-2728
Subject:
chlorins; chlorophyll; chloroplasts; electron transfer; embryophytes; fruits; leaves; metabolites; phototoxicity; ripening
Abstract:
... Chlorophyll breakdown is an important catabolic process of leaf senescence and fruit ripening. Structure elucidation of colorless linear tetrapyrroles as (final) breakdown products of chlorophyll was crucial for the recent delineation of a chlorophyll breakdown pathway which is highly conserved in land plants. Pheophorbide a oxygenase is the key enzyme responsible for opening of the chlorin macroc ...
DOI:
10.1016/j.bbabio.2010.12.007

95. Chlorophyll cycle regulates the construction and destruction of the light-harvesting complexes

Author:
Ryouichi Tanaka; Ayumi Tanaka
Source:
Biochimica et biophysica acta 2011 v.1807 no.8 pp. 968-976
ISSN:
0005-2728
Subject:
embryophytes; proteins; enzymes; photochemistry; electron transfer; Chlorophyta; genetic engineering; photosynthesis; chlorophyll; chloroplasts
Abstract:
... Chlorophyll a and chlorophyll b are the major constituents of the photosynthetic apparatus in land plants and green algae. Chlorophyll a is essential in photochemistry, while chlorophyll b is apparently dispensable for their photosynthesis. Instead, chlorophyll b is necessary for stabilizing the major light-harvesting chlorophyll-binding proteins. Chlorophyll b is synthesized from chlorophyll a an ...
DOI:
10.1016/j.bbabio.2011.01.002

96. Chloroplast-targeted ferredoxin-NADP⁺ oxidoreductase (FNR): Structure, function and location

Author:
Paula Mulo
Source:
Biochimica et biophysica acta 2011 v.1807 no.8 pp. 927-934
ISSN:
0005-2728
Subject:
electron transfer; electrons; flavin-adenine dinucleotide; genes; in vivo studies; molecular weight; photosynthesis; proteins; thylakoids
Abstract:
... Ferredoxin-NADP⁺ oxidoreductase (FNR) is a ubiquitous flavin adenine dinucleotide (FAD)-binding enzyme encoded by a small nuclear gene family in higher plants. The chloroplast targeted FNR isoforms are known to be responsible for the final step of linear electron flow transferring electrons from ferredoxin to NADP⁺, while the putative role of FNR in cyclic electron transfer has been under discussi ...
DOI:
10.1016/j.bbabio.2010.10.001

97. Cholesterol and membrane phospholipid compositions modulate the leakage capacity of the swaposin domain from a potato aspartic protease (StAsp-PSI)

Author:
Fernando Muñoz; M. Francisca Palomares-Jerez; Gustavo Daleo; José Villalaín; M. Gabriela Guevara
Source:
Biochimica et biophysica acta 2011 v.1811 no.12 pp. 1038-1044
ISSN:
1388-1981
Subject:
aspartic proteinases; cell death; cholesterol; cytotoxicity; electrophoresis; gel chromatography; humans; hydrophobicity; lymphocytes; microorganisms; neoplasms; phospholipids; plasma membrane; potatoes; proteins; spectral analysis
Abstract:
... Potato aspartic proteases (StAPs) and their swaposin domain (StAsp-PSI) are proteins with cytotoxic activity which involves plasma membrane destabilization. The ability of these proteins to produce cell death varies with the cellular type. Therefore, StAPs and StAsp-PSI selective cytotoxicity could be attributed to the different membrane lipid compositions of target cells. In this work we investig ...
DOI:
10.1016/j.bbalip.2011.08.013

98. Choosing between glycolysis and oxidative phosphorylation: A tumor's dilemma?

Author:
Caroline Jose; Nadège Bellance; Rodrigue Rossignol
Source:
Biochimica et biophysica acta 2011 v.1807 no.6 pp. 552-561
ISSN:
0005-2728
Subject:
biogenesis; carcinogenesis; energy; glutamine; glycolysis; hypoxia; induced pluripotent stem cells; models; neoplasm cells; neoplasms; oncogenes; oxidative phosphorylation
Abstract:
... A considerable amount of knowledge has been produced during the last five years on the bioenergetics of cancer cells, leading to a better understanding of the regulation of energy metabolism during oncogenesis, or in adverse conditions of energy substrate intermittent deprivation. The general enhancement of the glycolytic machinery in various cancer cell lines is well described and recent analyses ...
DOI:
10.1016/j.bbabio.2010.10.012

99. Circulating biomarkers of protein oxidation for Alzheimer disease: Expectations within limits

Author:
Fabio Di Domenico; Raffaella Coccia; D. Allan Butterfield; Marzia Perluigi
Source:
Biochimica et biophysica acta 2011 v.1814 no.12 pp. 1785-1795
ISSN:
1878-1454
Subject:
Alzheimer disease; biomarkers; blood; brain; early diagnosis; oxidation; oxidative stress; patients; prediction; prognosis; proteins; proteomics; screening; tissues
Abstract:
... Alzheimer disease (AD), the most common dementing disorder, is a multifactorial disease with complex etiology. Among different hypotheses proposed for AD one of the most corroborated is the “oxidative stress hypothesis”. Although recent studies extensively demonstrated the specific oxidative modification of selected proteins in the brain of AD patients and how their dysfunction possibly correlates ...
DOI:
10.1016/j.bbapap.2011.10.001

100. Cleavage-resistant fusion proteins of the M₂ muscarinic receptor and Gαᵢ₁. Homotropic and heterotropic effects in the binding of ligands

Author:
Amy W.-S. Ma; John Y. Dong; Dengbo Ma; James W. Wells
Source:
Biochimica et biophysica acta 2011 v.1810 no.6 pp. 592-602
ISSN:
0304-4165
Subject:
G-protein coupled receptors; Western blotting; agonists; antagonists; binding properties; electrophoresis; ligands; proteolysis
Abstract:
... BACKGROUND: G protein-coupled receptors fused to a Gα-subunit are functionally similar to their unfused counterparts. They offer an intriguing view into the nature of the receptor–G protein complex, but their usefulness depends upon the stability of the fusion. METHODS: Fusion proteins of the M₂ muscarinic receptor and the α-subunit of Gᵢ₁ were expressed in CHO and Sf9 cells, extracted in digitoni ...
DOI:
10.1016/j.bbagen.2011.03.003