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- Gomez, Eduardo A., et al. Show all 8 Authors
- Biochimie 2015 v.112 pp. 49-56
- Escherichia coli; Lutzomyia ayacuchensis; blood coagulation; cDNA libraries; coagulation; collagen; complementary DNA; cutaneous leishmaniasis; cysteine; dose response; enzyme activity; fibrinogen; hematophagy; hemostasis; integrins; recombinant proteins; salivary glands; sequence analysis
- ... Sequence analysis of the Lutzomyia (Lu.) ayacuchensis salivary gland cDNA library identified a short peptide containing an RGD (Arg-Gly-Asp) sequence flanked by two cysteine residues in the C-terminal end as the most abundant transcript. In the present study, a recombinant protein of the RGD-containing peptide, designated ayadualin, was expressed in Escherichia coli and its activity was characteri ...