U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Journal name Biologia plantarum Remove constraint Journal name: Biologia plantarum Publication year rev 7992-2008 Remove constraint Publication year rev: 7992-2008 Source 2008 v.52 no.2 Remove constraint Source: 2008 v.52 no.2
Start Over

Search

33  Search Results

« Previous | 1 - 20 of 33 |
Number of results to display per page

Search Results

1. Agrobacterium-mediated transformation in Citrullus lanatus

Author:
Cho, M. -A.; Moon, C. -Y.; Liu, J. -R.; Choi, P. -S.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 365-369
ISSN:
0006-3134
Subject:
Agrobacterium; Citrullus lanatus; Southern blotting; bacteria; beta-glucuronidase; explants; genes; glufosinate; greenhouses; phenotypic variation; polymerase chain reaction; shoots; transgenic plants; watermelons
Abstract:
... Agrobacterium tumefaciens-mediated transformation was used to produce transgenic watermelon. Cotyledonary explants of Citrullus lanatus Thumb (cv. Daesan) were co-cultivated with Agrobacterium strains (LBA4404, GV3101, EHA101) containing pPTN289 carrying with bar gene and pPTN290 carrying with nptII gene, respectively. There was a significant difference in the transformation frequency between bact ...
DOI:
10.1007/s10535-008-0076-6

2. CONSTANS delays flowering and affects tuber yield in potato

Author:
González-Schain, N. D.; Suárez-López, P.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 251-258
ISSN:
0006-3134
Subject:
Arabidopsis thaliana; Solanum tuberosum; crop yield; flowering; flowers; gibberellic acid; phenotype; potatoes; stem elongation
Abstract:
... CONSTANS (CO) has a central role in the photoperiodic regulation of flowering in Arabidopsis thaliana. We show here that potato (Solanum tuberosum ssp. andigena) plants constitutively expressing Arabidopsis CO (pACo plants) flower late under all photoperiodic conditions tested. Exogenous application of gibberellic acid to pACo plants corrected their short stem phenotype but not their late flowerin ...
DOI:
10.1007/s10535-008-0054-z

3. Direct shoot regeneration from leaf explants of Spilanthes acmella

Author:
Saritha, K. V.; Naidu, C. V.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 334-338
ISSN:
0006-3134
Subject:
Spilanthes; adventitious shoots; benzyladenine; buds; explants; flowering; gibberellic acid; indole acetic acid; indole butyric acid; isopentenyladenine; kinetin; leaves; naphthaleneacetic acid; organogenesis; plantlets; roots; seedlings; soil
Abstract:
... Multiple shoots of Spilanthes acmella Murr. were induced from nodal buds of in vivo and in vitro seedlings on Murashige and Skoog (MS) medium containing 1.0 mg dm⁻³ 6-benzyladenine (BA) and 0.1 mg dm⁻³ α-naphthalene-acetic acid (NAA). Adventitious shoots were successfully regenerated from the leaf explants derived from the above mentioned multiple shoots. The efficiency of shoot regeneration was t ...
DOI:
10.1007/s10535-008-0068-6

4. Effect of wounding on chalcone synthase and pathogenesis related PR-10 gene expression and content of phenolic compounds in bilberry leaves

Author:
Jaakola, L.; Koskimäki, J. J.; Riihinen, K. R.; Tolvanen, A.; Hohtola, A.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 391-395
ISSN:
0006-3134
Subject:
biosynthesis; coumaric acids; cutting; flavonols; gene expression; genes; glycosides; leaves; naringenin-chalcone synthase; pathogenesis; proanthocyanidins; shoots
Abstract:
... The influence of artificial wounding on biosynthesis of flavonoids and hydroxycinnamic acids was studied in bilberry leaves using two separate wounding experiments. In the first experiment bilberry leaves were wounded by cutting. The expression of the first gene from flavonoid pathway, chalcone synthase (CHS) and a wound induced pathogenesis related PR-10 gene was analysed from samples collected i ...
DOI:
10.1007/s10535-008-0082-8

5. Generation of low copy number and stably expressing transgenic creeping bentgrass plants using minimal gene cassette bombardment

Author:
Jayaraj, J.; Liang, G. H.; Muthukrishnan, S.; Punja, Z. K.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 215-221
ISSN:
0006-3134
Subject:
Agrostis stolonifera; Southern blotting; gene silencing; green fluorescent protein; nucleotide sequences; plasmid vectors; plasmids; transgenes; transgenic plants
Abstract:
... A minimal gene cassette comprised of the ubiquitin (Ubi) promoter + green fluorescent protein (Gfp) gene + Nos terminator DNA sequences, derived from the plasmid vector pPZP201-Gfp was utilized for transformation of creeping bentgrass using particle bombardment. Bentgrass calli bombarded individually with equivalent amounts of the cassette or whole plasmid DNA were compared for Gfp expression and ...
DOI:
10.1007/s10535-008-0048-x

6. Glutathione and phytochelatin contents in tomato plants exposed to cadmium

Author:
Ben Ammar, W.; Mediouni, C.; Tray, B.; Ghorbel, M. H.; Jemal, F.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 314-320
ISSN:
0006-3134
Subject:
Solanum lycopersicum var. lycopersicum; cadmium; culture media; glutathione; leaves; phytochelatins; roots; tomatoes
Abstract:
... The effect of cadmium on growth and contents of glutathione (GSH) and phytochelatins (PCs) were investigated in roots and leaves of tomato plants (Lycopersicon esculentum Mill. cv. 63/5 F1). The accumulation of Cd increased with external Cd concentrations and was considerably higher in roots than in leaves. Dry mass production decreased under Cd treatment especially in leaves. In both roots and le ...
DOI:
10.1007/s10535-008-0065-9

7. Leaf gas exchange, water relations, nutrient content and growth in citrus and olive seedlings under salinity

Author:
Melgar, J. C.; Syvertsen, J. P.; Martínez, V.; García-Sánchez, F.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 385-390
ISSN:
0006-3134
Subject:
Citrus limonia; Olea europaea; carbon dioxide; chlorophyll; fluorescence; gas exchange; greenhouses; leaves; mesophyll; nutrient content; olives; osmotic stress; photosynthesis; roots; salinity; seedlings; sodium; sodium chloride; stomatal conductance; toxicity
Abstract:
... The effects of salinity on growth, leaf nutrient content, water relations, gas exchange parameters and chlorophyll fluorescence were studied in six-month-old seedlings of citrus (Citrus limonia Osbeck) and rooted cuttings of olive (Olea europaea L. cv. Arbequina). Citrus and olive were grown in a greenhouse and watered with half strength Hoagland's solution plus 0 or 50 mM NaCl for citrus, or plus ...
DOI:
10.1007/s10535-008-0081-9

8. Stem CO₂ efflux of a Populus nigra stand: effects of elevated CO₂, fertilization, and shoot size

Author:
Liberloo, M.; De Angelis, P.; Ceulemans, R.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 299-306
ISSN:
0006-3134
Subject:
Populus nigra; carbon; carbon dioxide; coppicing; free air carbon dioxide enrichment; growing season; models; nitrogen fertilizers; nutrients; prediction; sap flow; shoots; temperature; trees
Abstract:
... To determine whether long-term growth in elevated atmospheric CO₂ concentration [CO₂] and nitrogen fertilization affects woody tissue CO₂ efflux, we measured stem CO₂ efflux as a function of temperature in three different size classes of shoots of Populus nigra L. (clone Jean Pourtet) on two occasions in 2004. Trees were growing in a short rotation coppice in ambient (370 μmol mol⁻¹) and elevated ...
DOI:
10.1007/s10535-008-0063-y

9. Stress-induced degradation of D1 protein and its photoprotection by DCPIP in isolated thylakoid membranes of barley leaf

Author:
Pandey, D. M.; Yeo, U. -D.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 291-298
ISSN:
0006-3134
Subject:
D1 protein; barley; electron transfer; hydrogen peroxide; leaves; light intensity; oxygen; photostability; sodium chloride; thylakoids
Abstract:
... Effects of various stress treatments such as NaCl, hydrogen peroxide, hydroxyl free radical, and high irradiance (HI, 1 000 μmol m⁻² s⁻¹) on the photosystem (PS) 2 mediated electron transport rate and the degradation of D1 protein in the thylakoid membranes of barley were studied. The applied stresses caused significant reduction in the PS 2-mediated electron transport and a degradation of D1 prot ...
DOI:
10.1007/s10535-008-0061-0

10. A novel cadmium induced protein in wheat: characterization and localization in root tissue

Author:
Mittra, B.; Sharma, S.; Das, A. B.; Henry, S. L.; Das, T. K.; Ghosh, P.; Ghosh, S.; Mohanty, P.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 343-346
ISSN:
0006-3134
Subject:
Triticum aestivum; cadmium; databases; expressed sequence tags; germination; phytotoxicity; plasma membrane; polyclonal antibodies; seedlings; seeds; tonoplast; wheat; wheat protein
Abstract:
... A 51-kDa soluble protein was over-expressed in wheat (Triticum aestivum) seedlings by the treatment of seeds before germination with 50 μM CdCl₂ for 48 h and subsequently washed off Cd²⁺. This protein designated as Cd stress associated protein (CSAP), was purified. Polyclonal antibody was raised against CSAP for localizing the protein in root tissue of treated and control seedlings. It was observe ...
DOI:
10.1007/s10535-008-0070-z

11. Changes in activities of antioxidant enzymes during Chenopodium murale seed germination

Author:
Bogdanović, J.; Radotić, K.; Mitrović, A.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 396-400
ISSN:
0006-3134
Subject:
Chenopodium murale; catalase; enzyme activity; gibberellic acid; imbibition; isozymes; peroxidase; seed germination; seedlings; seeds; superoxide dismutase
Abstract:
... The activities and isoenzyme pattern of catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD) have been studied during germination of Chenopodium murale seeds. CAT and SOD activities were similar in dry seeds and during first 2 d of imbibition. CAT activity increased during radicle protrusion and early seedling development. The maximum SOD activity was found at final stages of germinatio ...
DOI:
10.1007/s10535-008-0083-7

12. Cloning and quantification of expression levels of two MADS-box genes from Momordica charantia

Author:
Peng, S. M.; Luo, T.; Zhou, J. Y.; Niu, B.; Lei, N. F.; Tang, L.; Chen, F.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 222-230
ISSN:
0006-3134
Subject:
Momordica charantia; amino acid sequences; amino acids; complementary DNA; conserved sequences; females; flowering; flowers; gene expression regulation; genes; nucleotide sequences; phylogeny; reverse transcriptase polymerase chain reaction; sequence analysis
Abstract:
... MADS-box genes are known to be important for the development of flowers. Two MADS-box genes (MCAG2 and MCAG6) were isolated from the bitter gourd (Momordica charantia) female bud based on the MADS-box conserved sequences. The complete cDNA sequences of MCAG2 and MCAG6 encode a 231 and a 247 amino acid protein, respectively. Sequence comparison and phylogenetic analysis showed that MCAG2 and MCAG6 ...
DOI:
10.1007/s10535-008-0049-9

13. Cryopreservation of cell suspension cultures of Taxus x media and Taxus floridana

Author:
Škrlep, K.; Bergant, M.; De Winter, G. M.; Bohanec, B.; Žel, J.; Verpoorte, R.; Van Iren, F.; Camloh, M.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 329-333
ISSN:
0006-3134
Subject:
Taxus media; cell culture; cell lines; cell viability; cooling; cryopreservation; cryoprotectants; culture media; dimethyl sulfoxide; freezers; freezing; glycerol; ice; mannitol; nitrogen; nuclear genome; sucrose
Abstract:
... Different lines of cell suspension cultures of Taxus x media Rehd. and Taxus floridana Nutt. were cryopreserved with a two-step freezing method using a simple and inexpensive freezing container instead of a programmable freezer. Four to seven days old suspension cell cultures were precultured in growth medium supplemented with 0.5 M mannitol for 2 d. The medium was then replaced with cryoprotectan ...
DOI:
10.1007/s10535-008-0067-7

14. Direct organogenesis from leaf explants of Stevia rebaudiana and cultivation in bioreactor

Author:
Sreedhar, R. V.; Venkatachalam, L.; Thimmaraju, R.; Bhagyalakshmi, N.; Narayan, M. S.; Ravishankar, G. A.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 355-360
ISSN:
0006-3134
Subject:
Stevia rebaudiana; agar; biomass; bioreactors; buds; explants; gardens; greenhouses; horticultural soils; indole butyric acid; kinetin; leaves; organogenesis; rooting; salts; sand; shoots; sucrose; vermicomposts
Abstract:
... Shoot buds were induced directly on either side of midrib from adaxial surface of immature leaf explants in Stevia rebaudiana Bertoni five weeks after culturing in Murashige and Skoog's nutrient medium supplemented with 8.88 μM of N ⁶-benzylaminopurine and kinetin ranging from 4.65 to 6.98 μM. Immature leaves of 0.6 to 1 cm were found to produce best response (93 %) with a highest number of 4.93 s ...
DOI:
10.1007/s10535-008-0073-9

15. Efficient regeneration from hypocotyl explants in three cotton cultivars

Author:
Divya, K.; Swathi Anuradha, T.; Jami, S. K.; Kirti, P. B.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 201-208
ISSN:
0006-3134
Subject:
activated carbon; cotton; cultivars; culture media; cytokinins; ethylene inhibitors; explants; hypocotyls; indole butyric acid; kinetin; naphthaleneacetic acid; rooting; seedlings; shoot primordia; silver nitrate; thidiazuron; vitamins
Abstract:
... A high frequency in vitro shoot bud differentiation and multiple shoot production protocol from hypocotyl segments of 8 to 10-d-old seedlings of cotton has been developed. Murashige and Skoog (MS) basal medium with Nitsch and Nitsch vitamins was found to be optimal in shoot regeneration. A combination of 2 mg dm⁻³ thidiazuron and 0.05 mg dm⁻³ naphthaleneacetic acid was the most effective for shoot ...
DOI:
10.1007/s10535-008-0046-z

16. Expression of tea cytosolic glutamine synthetase is tissue specific and induced by cadmium and salt stress

Author:
Rana, N. K.; Mohanpuria, P.; Yadav, S. K.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 361-364
ISSN:
0006-3134
Subject:
Camellia sinensis; aluminum; cadmium; cold; copper; drought; glutamate-ammonia ligase; heat stress; leaves; roots; salt stress; tea
Abstract:
... Glutamine synthetase (GS) showed highest expression and activity in bud (youngest topmost leaf) of Camellia sinensis, lower in older leaves, while lowest activity in stem and roots. GS expression and activity was increased by ammonium and nitrate and also by cadmium and salt stress but decreased by copper, aluminum, drought, cold and heat stress. ...
DOI:
10.1007/s10535-008-0075-7

17. Expression of the ndhCKJ operon of barley and editing at the 13th base of the mRNA of the ndhC gene

Author:
Serrot, P. H.; Sabater, B.; Martín, M.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 347-350
ISSN:
0006-3134
Subject:
Liliopsida; Magnoliopsida; Marchantia; barley; complementary DNA; cytosine; histidine; messenger RNA; nucleotide sequences; operon; oxidative stress; paraquat; plastid DNA; polypeptides; thylakoids; tyrosine; uracil
Abstract:
... We have determined a 1778 base sequence which includes the complete ndhCKJ operon of barley plastid DNA. This operon contains the ndhC, ndhK and ndhJ genes encoding the polypeptides NDH-C, NDH-K and NDH-J, respectively, of the thylakoid Ndh complex. Poly-and mono-cistronic transcripts were identified, with an increase in the latter under oxidative stress induced by herbicide Paraquat. Complete seq ...
DOI:
10.1007/s10535-008-0071-y

18. In vitro regeneration of medicinal plant Centella asiatica

Author:
Mohapatra, H.; Barik, D. P.; Rath, S. P.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 339-342
ISSN:
0006-3134
Subject:
Centella asiatica; callus formation; chlorophyll; in vitro regeneration; indole butyric acid; leaves; medicinal plants; naphthaleneacetic acid; plantlets; proteins; reducing sugars; rooting; roots; shoots; soil
Abstract:
... This paper describes multiple shoot regeneration from leaf and nodal segments of a medicinally important herb Centella asiatica L. on Murashige and Skoog's (MS) medium supplemented with a range of growth regulators. The highest number of multiple shoots was observed on MS augmented with 3.0 mg dm⁻³ N⁶-benzylaminopurine (BAP) and 0.05 mg dm⁻³ α-naphthaleneacetic acid (NAA). Leaf explant showed maxi ...
DOI:
10.1007/s10535-008-0069-5

19. In vivo activity of recombinant human lewis fucosyltransferase III in leaves of Nicotiana tabacum L

Author:
Lhernould, S.; Labrousse, P.; Lejolly, D.; Léonard, R.; Carlué, M.; Costa, G.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 267-274
ISSN:
0006-3134
Subject:
Nicotiana tabacum; Western blotting; gene overexpression; genetically modified organisms; glycoproteins; humans; leaves; mass spectrometry; polysaccharides; tobacco
Abstract:
... Fucosylation in plants occurs in glycoproteins and polysaccharides but the function of fucosylation is largely unknown. We aimed to analyze the effects of over-expression of human fucosyltransferase III (hFucT III) on in vivo N-glycan accumulation in tobacco plant leaves and focused on comparing the amount of Lewis a (Lea)-epitope accumulation in transgenic and in wild-type plants. Fucosyltransfer ...
DOI:
10.1007/s10535-008-0057-9

20. Induction of pathogenesis-related proteins in sugarcane leaves and cell-cultures by a glycoprotein elicitor isolated from Colletotrichum falcatum

Author:
Ramesh Sundar, A.; Velazhahan, R.; Nagarathinam, S.; Vidhyasekaran, P.
Source:
Biologia plantarum 2008 v.52 no.2 pp. 321-328
ISSN:
0006-3134
Subject:
cultivars; barley; Saccharum officinarum; beans; polyacrylamide gel electrophoresis; chitinase; Western blotting; beta-glucanase; elicitors; cultured cells; pathogens; sugarcane; antiserum; molecular weight; glycoproteins; leaves; Glomerella tucumanensis
Abstract:
... The induction of pathogenesis-related (PR) proteins in sugarcane (Saccharum officinarum L.) leaves and suspension-cultured cells in response to treatment with a glycoprotein elicitor isolated from Colletotrichum falcatum (the red rot pathogen) was investigated. Treatment of leaves and cells with the elicitor resulted in a much marked increase in the activities of chitinase and β-1,3-glucanase in r ...
DOI:
10.1007/s10535-008-0066-8