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You searched for: Journal name Biologia plantarum Remove constraint Journal name: Biologia plantarum Publication year rev 7993-2007 Remove constraint Publication year rev: 7993-2007 Source 2007 v.51 no.1 Remove constraint Source: 2007 v.51 no.1
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1. Accumulation and detoxification of cadmium in Brassica pekinensis and B. chinensis

Author:
C. P. Liu; Z. G. Shen; X. D. Li
Source:
Biologia plantarum 2007 v.51 no.1 pp. 116-120
ISSN:
0006-3134
Subject:
Brassica rapa subsp. chinensis; Brassica rapa subsp. pekinensis; cadmium; cell walls; chlorophyll; green leafy vegetables; hydroponics; leaves; root growth; roots; shoots; thiols
Abstract:
... The effect of excessive Cd on the growth and metal uptake by leafy vegetables Brassica chinensis L. (cv. Wuyueman) and Brassica pekinensis (Lour.) Rupr. (cv. Qingyan 87-114) were studied in hydroponic solution culture. The Cd concentration higher than 10 µM significantly decreased the root elongation and leaf chlorophyll contents of both plant species. The shoots of B. pekinensis had significantly ...
DOI:
10.1007/s10535-007-0023-y

2. Agrobacterium tumefaciens-mediated transformation of blackgram: An assessment of factors influencing the efficiency of uidA gene transfer

Author:
R. Saini; P. K. Jaiwal
Source:
Biologia plantarum 2007 v.51 no.1 pp. 69-74
ISSN:
0006-3134
Subject:
gene transfer; kanamycin; seeds; Vigna mungo; kanamycin kinase; coculture; Agrobacterium radiobacter; black gram; explants; polymerase chain reaction; seedlings; pollen; soil; virulence; introns; roots; beta-glucuronidase; leaves; shoots
Abstract:
... Agrobacterium tumefaciens strain EHA105 carrying a binary vector pCAMBIA2301, which contains a neomycin phosphotransferase gene (nptII) and a β-glucuronidase (GUS) gene (uidA) interrupted with an intron, was used for transformation of Vigna mungo cotyledonary node explants. Various factors such as preculture and wounding of explants, manipulations in inoculation and co-cultivation conditions were ...
DOI:
10.1007/s10535-007-0014-z

3. Agrobacterium-mediated transformation of indica rice with chitinase gene for enhanced sheath blight resistance

Author:
R. Nandakumar; S. Babu; K. Kalpana; T. Raguchander; P. Balasubramanian; R. Samiyappan
Source:
Biologia plantarum 2007 v.51 no.1 pp. 142-148
ISSN:
0006-3134
Subject:
Thanatephorus cucumeris; bioassays; blight; callus; chitinase; complementary DNA; cultivars; explants; gene transfer; genotype; greenhouses; inheritance (genetics); leaves; pathogens; polymerase chain reaction; rice; transgenes; transgenic plants
Abstract:
... Four rice indica genotypes of local importance were transformed with RC7, rice chitinase cDNA clone through Agrobacterium-mediated gene transfer method using mature seed derived calli as explants. The putative hygromycin resistant calli showed varied level of regeneration efficiency ranging from 2.0 to 7.6 %. The stable integration and expression of RC7 was confirmed through polymerase chain react ...
DOI:
10.1007/s10535-007-0027-7

4. Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay

Author:
P. Joshi; V. Dhawan
Source:
Biologia plantarum 2007 v.51 no.1 pp. 22-26
ISSN:
0006-3134
Subject:
Swertia chirayita; micropropagation; microsatellite repeats; plantlets
Abstract:
... Inter simple sequence repeat (ISSR) marker assay was employed to validate the genetic fidelity of Swertia chirayita plantlets multiplied in vitro by axillary multiplication upto forty-two passages. Sixteen ISSR primers generated a total of 102 amplicons among the tissue-cultured plants. Forty-eight amplicons were amplified in the outlier (a Swertia species). The outlier (negative control) was empl ...
DOI:
10.1007/s10535-007-0005-0

5. Chitinase and peroxidase activities in sunflower hypocotyls: Effects of BTH and inoculation with Plasmopara halstedii

Author:
A. Roldán Serrano; J. Luna del Castillo; J. Jorrín Novo; A. Fernández Ocaña; M. V. Gómez Rodríguez
Source:
Biologia plantarum 2007 v.51 no.1 pp. 149-152
ISSN:
0006-3134
Subject:
Helianthus annuus; Plasmopara halstedii; chitinase; extracts; hypocotyls; pathogens; peroxidase; seedlings; systemic acquired resistance
Abstract:
... Systemic acquired resistance (SAR) can be induced in plants by incompatible pathogens, pathogen derived extracts, or certain chemicals as benzothiadiazole (BTH). The aim of this work was to compare changes in peroxidase and chitinase activities, enzymes considered as PR-proteins, caused by BTH and the pathogen Plasmopara halstedii. Hypocotyls from susceptible and resistant BTH-treated sunflower se ...
DOI:
10.1007/s10535-007-0028-6

6. Chromosome analysis and sorting in Vicia sativa using flow cytometry

Author:
P. Kovářová; A. Navrátilová; J. Macas; J. Doležel
Source:
Biologia plantarum 2007 v.51 no.1 pp. 43-48
ISSN:
0006-3134
Subject:
4',6-diamidino-2-phenylindole; DNA; Vicia sativa subsp. sativa; cell cycle; chromosomes; flow cytometry; fluorescence; formaldehyde; genome; homogenization; karyotyping; legumes; root tips; suspensions
Abstract:
... Procedures were developed for flow cytometric analysis and sorting of mitotic chromosomes (flow cytogenetics) of common vetch (Vicia sativa L., 2n=12). Suspensions of intact chromosomes were prepared from root tips after cell cycle synchronization, formaldehyde fixation, and mechanical homogenization. On average, 3 × 10⁵ morphologically intact chromosomes could be isolated from 25 root tips. Flow ...
DOI:
10.1007/s10535-007-0009-9

7. Developmental stage as a possible factor affecting cytokinin content and cytokinin dehydrogenase activity in Pinus sylvestris

Author:
A. E. Valdés; P. Galuszka; B. Fernández; M. L. Centeno; I. Frébort
Source:
Biologia plantarum 2007 v.51 no.1 pp. 193-197
ISSN:
0006-3134
Subject:
Pinus sylvestris; conifers; cytokinins; enzyme activity; enzyme substrates; isopentenyladenine; isozymes; pH; plantlets; seedlings; seeds
Abstract:
... In the present study cytokinin dehydrogenase (CKX) activity was for the first time found in a conifer species, Pinus sylvestris. The activities were correlated with the endogenous cytokinin contents. Several enzyme substrates and two different electron acceptors were used to search for the enzyme activity in the extract from seeds, seedlings and plantlets. The highest specific activity was found i ...
DOI:
10.1007/s10535-007-0039-3

8. Effect of NaCl and CaCl2 on growth and contents of minerals, chlorophyll, proline and sugars in the apple rootstock M 4 cultured in vitro

Author:
T. E. Sotiropoulos
Source:
Biologia plantarum 2007 v.51 no.1 pp. 177-180
ISSN:
0006-3134
Subject:
Malus domestica; apples; calcium chloride; chlorophyll; culture media; magnesium; minerals; nitrogen content; plantlets; potassium; proline; rootstocks; shoots; sodium; sodium chloride; sugars; zinc
Abstract:
... The apple (Malus domestica Borkh) rootstock M 4 shoots were grown in vitro for 4 weeks on Murashige and Skoog (MS) medium containing three NaCl concentrations (35, 100 and 200 mM) in combination with two CaCl₂ concentrations (5 and 10 mM). Inclusion of 10 mM CaCl₂ in the medium, in the presence of 35 mM NaCl, significantly increased the number of shoots and the fresh mass compared to 5 mM CaCl₂. T ...
DOI:
10.1007/s10535-007-0035-7

9. Effect of seed soaking with thiols on the antioxidant enzymes and photosystem activities in wheat subjected to water stress

Author:
N. S. Nathawat; J. S. Nair; S. M. Kumawat; N. S. Yadava; G. Singh; N. K. Ramaswamy; M. P. Sahu; S. F. D’Souza
Source:
Biologia plantarum 2007 v.51 no.1 pp. 93-97
ISSN:
0006-3134
Subject:
Triticum aestivum; antioxidant activity; antioxidants; dithiothreitol; enzyme activity; enzymes; leaves; photosystem I; polyethylene glycol; seedlings; seeds; soaking; solutions; thiols; thiourea; water stress; wheat
Abstract:
... Photosystem 1 and 2 and antioxidant enzyme activities were determined in wheat (Triticum aestivum L. cv. Sonalika) leaves. Seedlings from both control seeds and seeds soaked in solutions like dithiothreitol, thioglycollic acid and thiourea were subjected to water stress induced by polyethylene glycol. Photosystem 1 and 2 activities were less inhibited by water stress due to seed soaking with sulph ...
DOI:
10.1007/s10535-007-0019-7

10. Effects of sugars and growth regulators on in vitro growth of Dactylorhiza species

Author:
K. Wotavová-Novotná; H. Vejsadová; P. Kindlmann
Source:
Biologia plantarum 2007 v.51 no.1 pp. 198-200
ISSN:
0006-3134
Subject:
Dactylorhiza; adenine; cytokinins; glucose; indole butyric acid; naphthaleneacetic acid; root growth; roots; seedlings; shoots; sucrose
Abstract:
... The influence of sugars and growth regulators on shoot and root growth of Dactylorhiza species was studied under in vitro conditions. The seedling development was stimulated with the application of glucose and sucrose at concentration of 10 g dm⁻³ each. The improvement of shoot growth rate and shoot length was enhanced by cytokinins N ⁶-(2-isopentenyl)adenine or N ⁶-benzyladenine and their combina ...
DOI:
10.1007/s10535-007-0040-x

11. Estimation of endogenous contents of phytohormones during internode development in Merremia emarginata

Author:
D. Patel; V. S. Thaker
Source:
Biologia plantarum 2007 v.51 no.1 pp. 75-79
ISSN:
0006-3134
Subject:
Merremia emarginata; abscisic acid; enzyme-linked immunosorbent assay; gibberellic acid; hormones; indole acetic acid; polyclonal antibodies
Abstract:
... During the entire period of internode growth of Merremia emarginata contents of gibberellic acid (GA₃), phenyl-acetic acid (PAA), indole-3-acetic acid (IAA, free and conjugated) and abscisic acid (ABA, free and conjugated) were estimated by ELISA using polyclonal antibodies raised against each hormones. At the time of internode elongation free auxin content was low and increased with the decrease ...
DOI:
10.1007/s10535-007-0015-y

12. Expression of dehydrins under heat stress and their relationship with water relations of sugarcane leaves

Author:
A. Wahid; T. J. Close
Source:
Biologia plantarum 2007 v.51 no.1 pp. 104-109
ISSN:
0006-3134
Subject:
Saccharum officinarum; betaine; heat; heat stress; leaves; molecular weight; night temperature; proline; proteins; relative humidity; seedlings; sugarcane; sugars; water content
Abstract:
... The heat stress-induced dehydrin proteins (DHNs) expression and their relationship with the water relations of sugarcane (Saccharum officinarum L.) leaves were studied. Sugarcane seedlings were subjected to heat stress (day/night temperature of 40/35 °C) under relative humidity 60/65 % to avoid aerial desiccation and determinations made at 4, 12, 24, 36, 48, 60 and 72 h. The leaves showed a sharp ...
DOI:
10.1007/s10535-007-0021-0

13. Green fluorescent protein reveals variability in vacuoles of three plant species

Author:
G. P. Di Sansebastiano; L. Renna; M. Gigante; M. De Caroli; G. Piro; G. Dalessandro
Source:
Biologia plantarum 2007 v.51 no.1 pp. 49-55
ISSN:
0006-3134
Subject:
Nicotiana benthamiana; Nicotiana tabacum; callus; confocal microscopy; fluorescence; genome; green fluorescent protein; guard cells; plant characteristics; proteinases; protoplasts; transgenic plants; vacuoles
Abstract:
... Two vacuolar green fluorescent proteins (GFP) were stably inserted in Nicotiana tabacum and Nicotiana benthamiana genome, with unexpected difficulties, and compared with A. thaliana cv. Wassilewskaja transgenic plants expressing the same constructs. GFP fluorescence was strong in all tissues of A. thaliana but it was barely visible in Nicotiana. Confocal microscopy analysis revealed a variable dis ...
DOI:
10.1007/s10535-007-0010-3

14. Growth, abscisic acid content, and carbon isotope composition in wheat cultivars grown under different soil moisture

Author:
T. Wang; X. Zhang; C. Li
Source:
Biologia plantarum 2007 v.51 no.1 pp. 181-184
ISSN:
0006-3134
Subject:
abscisic acid; biomass; carbon; climate; cultivars; drought; dry matter partitioning; field capacity; isotopes; leaf area; leaves; roots; shoots; soil water; wheat
Abstract:
... Changes in dry matter accumulation and allocation, abscisic acid content and carbon isotope composition of three wheat cultivars from dry, middle and wet climate regions were recorded at full maturity after exposure to different watering regimes (100, 50 and 25 % field capacity). Compared with the wet climate cultivar, the dry climate cultivar showed lower stem height, total leaf area, total dry b ...
DOI:
10.1007/s10535-007-0036-6

15. Increased antioxidant activity in Cassia seedlings under UV-B radiation

Author:
S. Agarwal
Source:
Biologia plantarum 2007 v.51 no.1 pp. 157-160
ISSN:
0006-3134
Subject:
peroxidase; glutathione; Cassia; ultraviolet radiation; catalase; catechol oxidase; hydrogen peroxide; lipid peroxidation; phenols; seedlings; superoxide dismutase; antioxidant activity; chlorophyll; oxygen
Abstract:
... Cassia auriculata L. seedlings were irradiated with ultraviolet B (UV-B) in an environment-control chamber. The two doses assayed (7.5 and 15.0 kJ m⁻²) induced oxidative damage with an increase in lipid peroxidation and hydrogen peroxide and a decrease in chlorophyll and total phenol contents. The ascorbate and dehydroascorbate content as well as the reduced glutathione/oxidized glutathione conten ...
DOI:
10.1007/s10535-007-0030-z

16. Influence of UV-B radiation on young triticale plants with different wax cover

Author:
E. Skórska; W. Szwarc
Source:
Biologia plantarum 2007 v.51 no.1 pp. 189-192
ISSN:
0006-3134
Subject:
antioxidants; chlorophyll; cultivars; enzyme activity; fluorescence; leaves; photosynthesis; triticale; ultraviolet radiation
Abstract:
... Ultraviolet-B radiation (biologically effective dose 2.6 kJ m⁻² d⁻¹) had negative influence on morphology and physiology of the young triticale plants. Plants exposed to UV-B were of lower height than control plants, their leaves were narrow, and the rate of net photosynthesis was decreased. The line RAH 336, which wax cover is lesser than that of traditional cultivar Magnat, was more susceptible ...
DOI:
10.1007/s10535-007-0038-4

17. Kinetin regulates plant growth and biochemical changes during maturation and senescence of leaves, flowers, and pods of Cajanus cajan L.

Author:
D. Mukherjee; R. Kumar
Source:
Biologia plantarum 2007 v.51 no.1 pp. 80-85
ISSN:
0006-3134
Subject:
kinetin; plant growth; proteins; Cajanus cajan; DNA; leaf area; flowers; RNA; chlorophyll; enzyme activity; leaves; pods
Abstract:
... Aspects of plant growth such as height, branch number, leaf number, leaf area, pod area, 100-seed mass, etc., were correlated with biochemical changes such as contents of chlorophyll (Chl), proteins, DNA, and RNA, and protease activity during development and senescent phases in leaves, flowers, and pods of Cajanus cajan L. cv. UPAS-120 after treatments with kinetin (Kn). A significant increase was ...
DOI:
10.1007/s10535-007-0016-x

18. Lead-induced oxidative stress and metabolic alterations in Cassia angustifolia Vahl.

Author:
M. I. Qureshi; M. Z. Abdin; S. Qadir; M. Iqbal
Source:
Biologia plantarum 2007 v.51 no.1 pp. 121-128
ISSN:
0006-3134
Subject:
flowering; glutathione; glutathione-disulfide reductase; catalase; ascorbate peroxidase; superoxide dismutase; proline; thiobarbituric acid-reactive substances; Senna alexandrina; pot culture; lead acetate; oxidative stress
Abstract:
... Forty-five-days old plants of Indian senna (Cassia angustifolia Vahl.) were subjected to 0–500 µM lead acetate (Pb-Ac) in pot culture. Changes in contents of thiobarbituric acid reactive substances (TBARS), ascorbate, glutathione, proline, sennosides (a+b), and activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), and catalase (CAT) were studied at pre-f ...
DOI:
10.1007/s10535-007-0024-x

19. Mapping of QTLs affecting copper tolerance and the Cu, Fe, Mn and Zn contents in the shoots of wheat seedlings

Author:
A. F. Bálint; M. S. Röder; R. Hell; G. Galiba; A. Börner
Source:
Biologia plantarum 2007 v.51 no.1 pp. 129-134
ISSN:
0006-3134
Subject:
copper; greenhouse soils; iron; loci; manganese; quantitative trait loci; seedlings; shoots; wheat; zinc
Abstract:
... Quantitative trait loci (QTLs) for Cu-tolerance were determined in wheat grown in control and Cu-treated soil in greenhouse. In addition, loci having an influence on the shoot Cu-, Fe-, Mn-and Zn-contents under non-stressed and Cu-stressed environments were mapped. One major QTL for Cu-tolerance was found on chromosome 5DL, while slighter effects were determined on the chromosomes 1AL, 2DS, 4AL, 5 ...
DOI:
10.1007/s10535-007-0025-9

20. Micropropagation of Juniperus phoenicea from adult plant explants and analysis of ploidy stability using flow cytometry

Author:
J. Loureiro; A. Capelo; G. Brito; E. Rodriguez; S. Silva; G. Pinto; C. Santos
Source:
Biologia plantarum 2007 v.51 no.1 pp. 7-14
ISSN:
0006-3134
Subject:
Juniperus phoenicea; adults; assays; branches; buds; dipping; explants; flow cytometry; greenhouses; indole butyric acid; internodes; kinetin; mature plants; micropropagation; naphthaleneacetic acid; nuclear genome; olives; peat; perlite; ploidy; relative humidity; rooting; screening; shoots
Abstract:
... We report here the successful micropropagation of adult Juniperus phoenicea L. with respective ploidy stability studies. Microcuttings with axillary buds were grown on five media supplemented with different growth regulator combinations. Best elongation rates were achieved on Driver and Kuniyuki (DKW) medium supplemented with kinetin alone or with naphthaleneacetic acid (NAA), while Rugini olive ( ...
DOI:
10.1007/s10535-007-0003-2