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- Li, DeShan, et al. Show all 10 Authors
- Biologicals 2016 v.44 no.4 pp. 271-275
- DNA primers; Escherichia coli; Homo sapiens; antibodies; binding capacity; clones; enzyme-linked immunosorbent assay; flow cytometry; globulins; hepatitis B; hepatocytes; humans; mutants; neutralization; neutralization tests; polymerase chain reaction; rapid methods; screening
- ... A CDR3 mutant library was constructed from a previously isolated anti-HBV neutralizing Homo sapiens scFv-31 template by random mutant primers PCR. Then the library was displayed on the inner membrane surface in Escherichia coli periplasmic space. Seven scFv clones were isolated from the mutant library through three rounds of screening by flow cytometry. Competition ELISA assay indicates that isola ...