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You searched for: Journal name Biomaterials Remove constraint Journal name: Biomaterials Publication year rev 7988-2012 Remove constraint Publication year rev: 7988-2012 Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. A 3-D organoid kidney culture model engineered for high-throughput nephrotoxicity assays

Author:
Anna I. Astashkina; Brenda K. Mann; Glenn D. Prestwich; David W. Grainger
Source:
Biomaterials 2012 v.33 no.18 pp. 4700-4711
ISSN:
0142-9612
Subject:
biomarkers; biomimetics; cell culture; cultured cells; drugs; metabolism; models; nephrotoxicity; pharmacology; phenotype; plastics; proximal tubules; screening; toxicology
Abstract:
... Cell–cell and cell-matrix interactions control cell phenotypes and functions in vivo. Maintaining these interactions in vitro is essential to both produce and retain cultured cell fidelity to normal phenotype and function in the context of drug efficacy and toxicity screening. Two-dimensional (2-D) cultures on culture plastics rarely recapitulate any of these desired conditions. Three dimensional ...
DOI:
10.1016/j.biomaterials.2012.02.063

2. 3D co-culture of hematopoietic stem and progenitor cells and mesenchymal stem cells in collagen scaffolds as a model of the hematopoietic niche

Author:
Isabelle Leisten; Rafael Kramann; Mónica S. Ventura Ferreira; Manfred Bovi; Sabine Neuss; Patrick Ziegler; Wolfgang Wagner; Ruth Knüchel; Rebekka K. Schneider
Source:
Biomaterials 2012 v.33 no.6 pp. 1736-1747
ISSN:
0142-9612
Subject:
bone marrow; coculture; collagen; gels; hematopoiesis; homeostasis; migratory behavior; models; phenotype; stem cells; umbilical cord
Abstract:
... Here, we propose a collagen-based three-dimensional (3D) environment for hematopoietic stem and progenitor cells (HPC) with mesenchymal stem cells (MSC) derived either from bone marrow (BM) or umbilical cord (UC), to recapitulate the main components of the BM niche. Mechanisms described for HPC homeostasis were systematically analyzed in comparison to the conventional liquid HPC culture. The 3D-cu ...
DOI:
10.1016/j.biomaterials.2011.11.034

3. A 3D microfibrous scaffold for long-term human pluripotent stem cell self-renewal under chemically defined conditions

Author:
Hong Fang Lu; Karthikeyan Narayanan; Sze-Xian Lim; Shujun Gao; Meng Fatt Leong; Andrew C.A. Wan
Source:
Biomaterials 2012 v.33 no.8 pp. 2419-2430
ISSN:
0142-9612
Subject:
cell viability; encapsulation; humans; karyotyping; phenotype; stem cells; therapeutics
Abstract:
... Realizing the potential of human pluripotent stem cell (hPSC)-based therapy requires the development of defined scalable culture systems with efficient expansion, differentiation and isolation protocols. We report an engineered 3D microfiber system that efficiently supports long-term hPSCs self-renewal under chemically defined conditions. The unique feature of this system lies in the application o ...
DOI:
10.1016/j.biomaterials.2011.11.077

4. Activation of pluripotency-associated genes in mouse embryonic fibroblasts by non-viral transfection with in vitro-derived mRNAs encoding Oct4, Sox2, Klf4 and cMyc

Author:
Geertrui Tavernier; Katharina Wolfrum; Joseph Demeester; Stefaan C. De Smedt; James Adjaye; Joanna Rejman
Source:
Biomaterials 2012 v.33 no.2 pp. 412-417
ISSN:
0142-9612
Subject:
DNA; alkaline phosphatase; fibroblasts; gene expression regulation; genes; induced pluripotent stem cells; insertional mutagenesis; messenger RNA; mice; scientists; transcription factors; transfection
Abstract:
... The first successful reprogramming of differentiated cells to a pluripotent state was done by retroviral introduction of four transcription factors (Oct4, Sox2, Klf4, cMyc) by the group of Yamanaka in 2006. Since then, scientists all over the world have attempted various methods to avoid insertional mutagenesis, a major limitation of the retrovirus-based method, however no technique was found to c ...
DOI:
10.1016/j.biomaterials.2011.09.062

5. Adult and umbilical cord blood-derived platelet-rich plasma for mesenchymal stem cell proliferation, chemotaxis, and cryo-preservation

Author:
Matthew B. Murphy; Daniel Blashki; Rachel M. Buchanan; Iman K. Yazdi; Mauro Ferrari; Paul J. Simmons; Ennio Tasciotti
Source:
Biomaterials 2012 v.33 no.21 pp. 5308-5316
ISSN:
0142-9612
Subject:
adults; bioassays; bone marrow; cell proliferation; chemokine CCL5; chemotaxis; cultured cells; fetal bovine serum; humans; rats; stem cells; transforming growth factor beta 1; umbilical cord; vascular endothelial growth factors
Abstract:
... Platelet-rich plasma (PRP) was prepared from human adult peripheral blood and from human umbilical cord (uc) blood and the properties were compared in a series of in vitro bioassays. Quantification of growth factors in PRP and platelet-poor plasma (PPP) fractions revealed increased levels of mitogenic growth factors PDGF-AB, PDGF-BB, and FGF-2, the angiogenic agent VEGF and the chemokine RANTES in ...
DOI:
10.1016/j.biomaterials.2012.04.007

6. Adult stem cell-based tissue engineered blood vessels: A review

Author:
Jeffrey T. Krawiec; David A. Vorp
Source:
Biomaterials 2012 v.33 no.12 pp. 3388-3400
ISSN:
0142-9612
Subject:
adult stem cells; adults; blood vessels; bone marrow; cardiovascular diseases; embryonic stem cells; ethics; patients; tissue engineering
Abstract:
... With the high occurrence of cardiovascular disease and increasing numbers of patients requiring vascular access, there is a significant need for a small-diameter (<6 mm inner diameter) vascular graft that can provide long-term patency. Tissue engineering provides a very promising solution to this need. Approaches utilizing adult stem cells can address limitations previously realized in the use of ...
DOI:
10.1016/j.biomaterials.2012.01.014

7. Alendronate coated poly-lactic-co-glycolic acid (PLGA) nanoparticles for active targeting of metastatic breast cancer

Author:
Sanjay I. Thamake; Sangram L. Raut; Zygmunt Gryczynski; Amalendu P. Ranjan; Jamboor K. Vishwanatha
Source:
Biomaterials 2012 v.33 no.29 pp. 7164-7173
ISSN:
0142-9612
Subject:
bone resorption; breast neoplasms; curcumin; diffusivity; drug carriers; drug therapy; drugs; encapsulation; growth retardation; metastasis; models; myeloma; nanoparticles; neoplasm cells; osteoporosis; particle size; synergism
Abstract:
... Delivery of therapeutic agents to bone is crucial in several diseases such as osteoporosis, Paget’s disease, myeloproliferative diseases, multiple myeloma as well as skeletal metastasizing cancers. Prevention of cancer growth and lowering the cancer induced bone resorption is important in the treatment of bone metastasizing cancers. Keeping in mind the low diffusivity and availability of cell surf ...
DOI:
10.1016/j.biomaterials.2012.06.026

8. Alginate derivatization: A review of chemistry, properties and applications

Author:
Siddhesh N. Pawar; Kevin J. Edgar
Source:
Biomaterials 2012 v.33 no.11 pp. 3279-3305
ISSN:
0142-9612
Subject:
alginates; derivatization; drugs; hydrocolloids; hydroxyapatite; islets of Langerhans; nucleic acids; pH; proteins; temperature; value added
Abstract:
... Alginates have become an extremely important family of polysaccharides because of their utility in preparing hydrogels at mild pH and temperature conditions, suitable for sensitive biomolecules like proteins and nucleic acids, and even for living cells such as islets of Langerhans. In addition, the complex monosaccharide sequences of alginates, and our growing ability to create controlled sequence ...
DOI:
10.1016/j.biomaterials.2012.01.007

9. Alleviation of rheumatoid arthritis by cell-transducible methotrexate upon transcutaneous delivery

Author:
Sang-Won Lee; Ji-Hye Kim; Min-Chan Park; Yong-Beom Park; Wook Jin Chae; Tomohiro Morio; Dong-Ho Lee; Sang-Hwa Yang; Seung-Kyou Lee; Soo-Kon Lee; Sang-Kyou Lee
Source:
Biomaterials 2012 v.33 no.5 pp. 1563-1572
ISSN:
0142-9612
Subject:
blood serum; cartilage; cytotoxicity; methotrexate; mice; micro-computed tomography; oral administration; patients; rheumatoid arthritis; secretion; tumor necrosis factors
Abstract:
... Rheumatoid arthritis (RA) is a systemic autoimmune disease that is initiated and maintained by various inflammatory/immune cells and their cytokines, leading to cartilage degradation and bone erosion. Despite its potent therapeutic efficacy on RA, the oral administration of methotrexate (MTX) provokes serious adverse systemic complications, thus necessitating the local application of MTX. Here, we ...
DOI:
10.1016/j.biomaterials.2011.10.079

10. Amphiphilic micelles of poly(2-methyl-2-carboxytrimethylene carbonate-co-d,l-lactide)-graft-poly(ethylene glycol) for anti-cancer drug delivery to solid tumours

Author:
Karyn S. Ho; Ahmed M. Aman; Rima S. Al-awar; Molly S. Shoichet
Source:
Biomaterials 2012 v.33 no.7 pp. 2223-2229
ISSN:
0142-9612
Subject:
animal models; antineoplastic agents; breast neoplasms; drug delivery systems; ethylene glycol; micelles; nanoparticles; neoplasm cells; pharmacokinetics; polysorbates; solubility; solvents; surfactants; taxanes; tissues; toxicity
Abstract:
... Drug delivery to solid tumours remains a challenge because both tumour physiology and drug solubility are unfavourable. Engineered materials can provide the basis for drug reformulation, incorporating active compounds and modulating their pharmacokinetic and biodistribution behaviour. To this end, we encapsulated docetaxel, a poorly soluble taxane drug, in a self-assembled polymeric nanoparticle m ...
DOI:
10.1016/j.biomaterials.2011.11.072

11. Amphiphilic peptide carrier for the combined delivery of curcumin and plasmid DNA into the lungs

Author:
Ji Hwan Park; Hyun Ah Kim; Jin Hyeong Park; Minhyung Lee
Source:
Biomaterials 2012 v.33 no.27 pp. 6542-6550
ISSN:
0142-9612
Subject:
animal models; anti-inflammatory activity; curcumin; cytotoxicity; emulsions; evaporation; gel electrophoresis; heparin; hepatotoxicity; in vivo studies; intravenous injection; lipopolysaccharides; lungs; macrophages; mice; plasmids; solvents; tissues; transfection; tumor necrosis factor-alpha
Abstract:
... In this study, the R7L10 peptide, which is composed of a 7-arginine stretch and a 10-leucine stretch, was evaluated as a carrier for the combined delivery of curcumin and plasmid DNA (pDNA) into the lungs. Curcumin is a natural product with anti-inflammatory and anti-tumor effects. Curcumin-loaded R7L10 (R7L10-curucmin) was prepared by an oil-in-water (O/W) emulsion/solvent evaporation method. In  ...
DOI:
10.1016/j.biomaterials.2012.05.046

12. Angiogenic and osteogenic potential of platelet-rich plasma and adipose-derived stem cell laden alginate microspheres

Author:
Yi Man; Ping Wang; Yongwen Guo; Lin Xiang; Yang Yang; Yili Qu; Ping Gong; Li Deng
Source:
Biomaterials 2012 v.33 no.34 pp. 8802-8811
ISSN:
0142-9612
Subject:
blood vessels; cell movement; cell viability; encapsulation; in vivo studies; mineralization; stem cells; therapeutics
Abstract:
... Improving vascularization of tissue-engineered bone can advance cell performance in vivo and further promote bone regeneration. How to achieve a functional vascular network within the construct is one of the biggest challenges so far. We hypothesized that a mixture of platelet-rich plasma (PRP) and adipose-derived stem cells (ADSCs) could endue the alginate microspheres with osteogenic and angioge ...
DOI:
10.1016/j.biomaterials.2012.08.054

13. Anisamide-targeted cyclodextrin nanoparticles for siRNA delivery to prostate tumours in mice

Author:
Jianfeng Guo; Julien R. Ogier; Stephane Desgranges; Raphael Darcy; Caitriona O′Driscoll
Source:
Biomaterials 2012 v.33 no.31 pp. 7775-7784
ISSN:
0142-9612
Subject:
cyclodextrins; intravenous injection; luciferase; messenger RNA; mice; nanoparticles; neoplasm cells; prostatic neoplasms; reporter genes; small interfering RNA; toxicity; vascular endothelial growth factors
Abstract:
... A hepta-guanidino-β-cyclodextrin (G-CD), its hepta-PEG conjugate (G-CD-PEG), and the corresponding anisamide-terminated PEG conjugate (G-CD-PEG-AA) have been synthesised and compared as delivery vectors for siRNA to prostate cancer cells and tumours in vivo. The G-CD-PEG-AA.siRNA formulations (in which anisamide targets the sigma receptor), but not the non-targeted formulations, induced prostate c ...
DOI:
10.1016/j.biomaterials.2012.07.012

14. Anti-glioblastoma efficacy and safety of paclitaxel-loading Angiopep-conjugated dual targeting PEG-PCL nanoparticles

Author:
Hongliang Xin; Xianyi Sha; Xinyi Jiang; Wei Zhang; Liangcen Chen; Xiaoling Fang
Source:
Biomaterials 2012 v.33 no.32 pp. 8167-8176
ISSN:
0142-9612
Subject:
acute toxicity; blood-brain barrier; brain; drug delivery systems; endocytosis; intravenous injection; kidneys; liver; low density lipoprotein; nanoparticles; paclitaxel; permeability; physiological transport
Abstract:
... Therapeutic effect of glioma is often limited due to low permeability of delivery systems across the Blood-Brain Barrier (BBB) and poor penetration into the tumor tissue. In order to overcome the two barriers, we proposed Angiopep-conjugated PEG-PCL nanoparticles (ANG-PEG-NP) as a dual targeting drug delivery system for glioma treatment basing on low density lipoprotein receptor related protein (L ...
DOI:
10.1016/j.biomaterials.2012.07.046

15. Anti-inflammatory effect with high intensity focused ultrasound-mediated pulsatile delivery of diclofenac

Author:
Chih-Yu Wang; Chih-Hui Yang; Yung-Sheng Lin; Chih-Hsin Chen; Keng-Shiang Huang
Source:
Biomaterials 2012 v.33 no.5 pp. 1547-1553
ISSN:
0142-9612
Subject:
anti-inflammatory activity; drug delivery systems; drugs; edema; heat; in vitro studies; in vivo studies; irradiation; nanogold; rats; ultrasonics
Abstract:
... A pulsatile ultrasound controlled drug release platform with diclofenac-loaded alginate microcapsules (fabricated with a home-made electrostatic device, 75% embedded rate) was established to evaluate anti-inflammation efficiency. Better anti-inflammation efficiency was found using the ultrasound system and the drug delivery can be adjusted based on the programmed ultrasound cycle. The results of t ...
DOI:
10.1016/j.biomaterials.2011.10.047

16. Anti-tumor activity of paclitaxel through dual-targeting carrier of cyclic RGD and transferrin conjugated hyperbranched copolymer nanoparticles

Author:
Qing Xu; Yuexian Liu; Shishuai Su; Wei Li; Chunying Chen; Yan Wu
Source:
Biomaterials 2012 v.33 no.5 pp. 1627-1639
ISSN:
0142-9612
Subject:
anticarcinogenic activity; biodegradability; composite polymers; cytotoxicity; drug therapy; drugs; encapsulation; endocytosis; fluorescence; human umbilical vein endothelial cells; humans; integrins; monitoring; nanocarriers; nanoparticles; neoplasm cells; paclitaxel; transferrin; uterine cervical neoplasms
Abstract:
... Targeted delivery strategies are becoming increasingly important. Herein, a novel hyperbranched amphiphilic poly[(amine-ester)-co-(d,l-lactide)]/1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine copolymer (HPAE-co-PLA/DPPE) with RGD peptide (cRGDfK) and transferrin (Tf) on the periphery was synthesized and used to prepare paclitaxel-loaded nanoparticles (NPs) for dual-targeting chemotherapy. These ...
DOI:
10.1016/j.biomaterials.2011.11.012

17. Antibacterial and antifouling catheter coatings using surface grafted PEG-b-cationic polycarbonate diblock copolymers

Author:
Xin Ding; Chuan Yang; Tze Peng Lim; Li Yang Hsu; Amanda C. Engler; James L. Hedrick; Yi-Yan Yang
Source:
Biomaterials 2012 v.33 no.28 pp. 6593-6603
ISSN:
0142-9612
Subject:
Staphylococcus aureus; X-ray photoelectron spectroscopy; adhesion; adsorption; antibiotic resistance; biofilm; blood proteins; coatings; composite polymers; contact angle; fouling; hemolysis; hydrophobicity; methicillin; morbidity; mortality; polymerization; quartz; rubber; scanning electron microscopy; silicone
Abstract:
... Intravascular catheter-associated infections (CAIs), which are normally induced by microbial adhesion and subsequent biofilm formation, are a major cause of morbidity and mortality. Therefore, strategies to prevent CAIs are in great demand. In this study, a series of diblock copolymers of PEG and cationic polycarbonate with various compositions were synthesized by metal-free organocatalytic ring-o ...
DOI:
10.1016/j.biomaterials.2012.06.001

18. Antisense inhibition of gene expression and growth in gram-negative bacteria by cell-penetrating peptide conjugates of peptide nucleic acids targeted to rpoD gene

Author:
Hui Bai; Yu You; Hua Yan; Jingru Meng; Xiaoyan Xue; Zheng Hou; Ying Zhou; Xue Ma; Guojun Sang; Xiaoxing Luo
Source:
Biomaterials 2012 v.33 no.2 pp. 659-667
ISSN:
0142-9612
Subject:
DNA-directed RNA polymerase; Escherichia coli; Gram-negative bacteria; Klebsiella pneumoniae; Salmonella enterica; Shigella flexneri; anti-infective properties; antibiotic resistance; antibiotics; epithelial cells; gene expression; genes; human growth; humans; messenger RNA; microbial growth; models; mortality; multiple drug resistance; nucleotide sequences
Abstract:
... Gram-negative bacteria (GNB) cause common and severe hospital- and community-acquired infections with a high incidence of multidrug resistance (MDR) and mortality. The emergence and spread of MDR-GNB strains limit therapeutic options and highlight the need to develop new therapeutic strategies. In this study, the peptide (RXR)₄XB- and (KFF)₃K-conjugated peptide nucleic acids (PPNAs) were developed ...
DOI:
10.1016/j.biomaterials.2011.09.075

19. Antitumor efficacy following the intracellular and interstitial release of liposomal doxorubicin

Author:
Amey Bandekar; Shrirang Karve; Min-Yuan Chang; Qingshan Mu; Jimmy Rotolo; Stavroula Sofou
Source:
Biomaterials 2012 v.33 no.17 pp. 4345-4352
ISSN:
0142-9612
Subject:
breast neoplasms; doxorubicin; drug therapy; encapsulation; humans; mice; models; neoplasm cells; pH; prognosis
Abstract:
... pH-triggered lipid-membranes designed from biophysical principles are evaluated in the form of targeted liposomal doxorubicin with the aim to ultimately better control the growth of vascularized tumors. We compare the antitumor efficacy of anti-HER2/neu pH-triggered lipid vesicles encapsulating doxorubicin to the anti-HER2/neu form of an FDA approved liposomal doxorubicin of DSPC/cholesterol-based ...
DOI:
10.1016/j.biomaterials.2012.02.039

20. Aptamer-conjugated and drug-loaded acoustic droplets for ultrasound theranosis

Author:
Chung-Hsin Wang; Shih-Tsung Kang; Ya-Hsuan Lee; Yun-Ling Luo; Yu-Fen Huang; Chih-Kuang Yeh
Source:
Biomaterials 2012 v.33 no.6 pp. 1939-1947
ISSN:
0142-9612
Subject:
acoustics; cell viability; chemical treatment; doxorubicin; droplets; image analysis; neoplasm cells; oligonucleotides; therapeutics; toxicity; ultrasonics; ultrasonography; volatilization
Abstract:
... Tumor therapy requires multi-functional treatment strategies with specific targeting of therapeutics to reduce general toxicity and increase efficacy. In this study we fabricated and functionally tested aptamer-conjugated and doxorubicin (DOX)-loaded acoustic droplets comprising cores of liquid perfluoropentane compound and lipid-based shell materials. Conjugation of sgc8c aptamers provided the ab ...
DOI:
10.1016/j.biomaterials.2011.11.036

21. Arginine functionalized peptide dendrimers as potential gene delivery vehicles

Author:
Kui Luo; Caixia Li; Li Li; Wenchuan She; Gang Wang; Zhongwei Gu
Source:
Biomaterials 2012 v.33 no.19 pp. 4917-4927
ISSN:
0142-9612
Subject:
arginine; atomic force microscopy; biosafety; blood serum; breast neoplasms; cytotoxicity; gene therapy; genes; in vitro studies; light scattering; models; molecular weight; plasmids; transfection
Abstract:
... The quest for highly efficient and safe gene delivery systems has become the key factor for successful application of gene therapy. Peptide dendrimers are currently investigated as excellent candidates for non-viral gene delivery vectors. In this study, we report the synthesis and characterization of arginine functionalized peptide dendrimer-based vectors ranging from 5th generation (G5A) to 6th g ...
DOI:
10.1016/j.biomaterials.2012.03.030

22. Arginine-engrafted biodegradable polymer for the systemic delivery of therapeutic siRNA

Author:
Jagadish Beloor; Chang Seon Choi; Hye Yeong Nam; Minsun Park; Sung Hwa Kim; Andrew Jackson; Kuen Yong Lee; Sung Wan Kim; Priti Kumar; Sang-Kyung Lee
Source:
Biomaterials 2012 v.33 no.5 pp. 1640-1650
ISSN:
0142-9612
Subject:
RNA interference; acids; arginine; binding properties; biodegradability; body weight; cations; disulfide bonds; genes; nanoparticles; neoplasms; plasmids; polymers; small interfering RNA; therapeutics; vascular endothelial growth factors
Abstract:
... Small interfering RNA (siRNA) represent an interesting class of developmental nucleic acid-based therapeutics. Cationic carriers for deoxyribonucleic acids (DNA) are potential vehicles for siRNA delivery. However, in contrast to supercoiled plasmid DNA, the physical properties of siRNA molecules induces the formation of larger, loosely–packed complexes with most polycationic carriers, and conseque ...
DOI:
10.1016/j.biomaterials.2011.11.008

23. Au capped magnetic core/mesoporous silica shell nanoparticles for combined photothermo-/chemo-therapy and multimodal imaging

Author:
Ming Ma; Hangrong Chen; Yu Chen; Xia Wang; Feng Chen; Xiangzhi Cui; Jianlin Shi
Source:
Biomaterials 2012 v.33 no.3 pp. 989-998
ISSN:
0142-9612
Subject:
coatings; confocal laser scanning microscopy; cytotoxicity; doxorubicin; drug therapy; electrostatic interactions; gold; image analysis; irradiation; nanocomposites; nanoparticles; pH; silica; synergism
Abstract:
... Uniform Au NRs-capped magnetic core/mesoporous silica shell nanoellipsoids (Au NRs-MMSNEs) were prepared by coating a uniform layer of Au NRs on the outer surface of a magnetic core/mesoporous silica shell nanostructure, based on a two-step chemical self-assembly process. This multifunctional nanocomposite integrate simultaneous chemotherapy, photo-thermotherapy, in vivo MR-, infrared thermal and ...
DOI:
10.1016/j.biomaterials.2011.10.017

24. Augmented healing of critical-size calvarial defects by baculovirus-engineered MSCs that persistently express growth factors

Author:
Chin-Yu Lin; Yu-Han Chang; Chun-Yu Kao; Chia-Hsin Lu; Li-Yu Sung; Tzu-Chen Yen; Kun-Ju Lin; Yu-Chen Hu
Source:
Biomaterials 2012 v.33 no.14 pp. 3682-3692
ISSN:
0142-9612
Subject:
X-radiation; angiogenesis; bone formation; engineering; rabbits; stem cells; vascular endothelial growth factors
Abstract:
... Repair of large calvarial bony defects remains clinically challenging because successful spontaneous calvarial re-ossification rarely occurs. Although bone marrow-derived mesenchymal stem cells (BMSCs) genetically engineered with baculovirus (BV) for transient expression of osteogenic/angiogenic factors hold promise for bone engineering, we hypothesized that calvarial bone healing necessitates pro ...
DOI:
10.1016/j.biomaterials.2012.02.007

25. Automated microinjection of cell-polymer suspensions in 3D ECM scaffolds for high-throughput quantitative cancer invasion screens

Author:
Hoa H. Truong; Jan de Sonneville; Veerander P.S. Ghotra; Jiangling Xiong; Leo Price; Pancras C.W. Hogendoorn; Herman H. Spaink; Bob van de Water; Erik H.J. Danen
Source:
Biomaterials 2012 v.33 no.1 pp. 181-188
ISSN:
0142-9612
Subject:
sarcoma; image analysis; cell movement; humans; cell aggregates; collagen; mice; gels; biopsy; droplets; algorithms; extracellular matrix
Abstract:
... Cell spheroids (CS) embedded in 3D extracellular matrix (ECM) serve as in vitro mimics for multicellular structures in vivo. Such cultures, started either from spontaneous cell aggregates or single cells dispersed in a gel are time consuming, applicable to restricted cell types only, prone to high variation, and do not allow CS formation with defined spatial distribution required for high-throughp ...
DOI:
10.1016/j.biomaterials.2011.09.049

26. BDNF blended chitosan scaffolds for human umbilical cord MSC transplants in traumatic brain injury therapy

Author:
Wei Shi; Dekang Nie; Guohua Jin; Weiwei Chen; Liang Xia; Xiujie Wu; Xing Su; Xide Xu; Lanchun Ni; Xianan Zhang; Xinhua Zhang; Jian Chen
Source:
Biomaterials 2012 v.33 no.11 pp. 3119-3126
ISSN:
0142-9612
Subject:
brain; cell differentiation; chitosan; coculture; crosslinking; cytotoxicity; enzyme-linked immunosorbent assay; fluorescence; humans; neurons; porosity; stem cells; therapeutics; tissue engineering; trypsin; umbilical cord; viability
Abstract:
... This study tested the cytotoxicity of a BDNF blended chitosan scaffold with human umbilical cord mesenchymal stem cells (hUC-MSCs), and the in vitro effect of BDNF blended chitosan scaffolds on neural stem cell differentiation with the aim of contributing alternative methods in tissue engineering for the treatment of traumatic brain injury (TBI). The chitosan scaffold based on immobilization of BD ...
DOI:
10.1016/j.biomaterials.2012.01.009

27. Balancing osteoblast functions and bacterial adhesion on functionalized titanium surfaces

Author:
Koon Gee Neoh; Xuefeng Hu; Dong Zheng; En Tang Kang
Source:
Biomaterials 2012 v.33 no.10 pp. 2813-2822
ISSN:
0142-9612
Subject:
alloys; bacteria; bacterial adhesion; bacterial colonization; dental prosthesis; orthopedics; osteoblasts; surface proteins; titanium
Abstract:
... The demand for orthopedic and dental implants will continue to grow, and for these applications, titanium and its alloys have been used extensively. While these implants have achieved high success rates, two major complications may be encountered: the lack of bone tissue integration and implant-centered infection. The surface of the implant, through its interactions with proteins, bacteria and tis ...
DOI:
10.1016/j.biomaterials.2012.01.018

28. Bifunctional combined Au-Fe₂O₃ nanoparticles for induction of cancer cell-specific apoptosis and real-time imaging

Author:
Wen Gao; Lifei Ji; Lu Li; Guanwei Cui; Kehua Xu; Ping Li; Bo Tang
Source:
Biomaterials 2012 v.33 no.14 pp. 3710-3718
ISSN:
0142-9612
Subject:
apoptosis; catalytic activity; fluorescein; fluorescence; gold; humans; hydroxyl radicals; image analysis; integrins; liver neoplasms; monitoring; nanoparticles; neoplasm cells
Abstract:
... We demonstrate bifunctional combined Au-Fe₂O₃ nanoparticles (NPs) for selectively induction of apoptosis in cancer cells and real-time imaging. The as-prepared Au-Fe₂O₃ NPs combine the merits of both Au and γ-Fe₂O₃ NPs, maintaining excellent fluorescence quenching property and catalytic activity. Conjugated with αⅤβ₃ integrin-targeting peptide (RGD) and fluorescein isothiocyanate (FITC)-labeled ca ...
DOI:
10.1016/j.biomaterials.2012.01.047

29. Binary and ternary complexes based on polycaprolactone-graft-poly (N, N-dimethylaminoethyl methacrylate) for targeted siRNA delivery

Author:
Yuanyu Huang; Daoshu Lin; Qian Jiang; Wendi Zhang; Shutao Guo; Ping Xiao; Shuquan Zheng; Xiaoxia Wang; Hongbo Chen; Hong-Yan Zhang; Liandong Deng; Jinfeng Xing; Quan Du; Anjie Dong; Zicai Liang
Source:
Biomaterials 2012 v.33 no.18 pp. 4653-4664
ISSN:
0142-9612
Subject:
RNA interference; Western blotting; blood; coatings; confocal microscopy; cytoplasm; cytotoxicity; fatty acids; fatty liver; fluorescence; folic acid; intravenous injection; liver; luciferase; lungs; mice; pancreas; research and development; secretion; small interfering RNA; tissues; uterine cervical neoplasms; zeta potential
Abstract:
... Small interfering RNA (siRNA) is a powerful gene silencing tool and has promising prospects in basic research and the development of therapeutic reagents. However, the lack of an effective and safe tool for siRNA delivery hampers its application. Here, we introduced binary and ternary complexes that effectively mediated siRNA-targeted gene silencing. Both complexes showed excellent siRNA loading e ...
DOI:
10.1016/j.biomaterials.2012.02.052

30. Biocleavable comb-shaped gene carriers from dextran backbones with bioreducible ATRP initiation sites

Author:
Zeng-Hui Wang; Yun Zhu; Ming-Ying Chai; Wan-Tai Yang; Fu-Jian Xu
Source:
Biomaterials 2012 v.33 no.6 pp. 1873-1883
ISSN:
0142-9612
Subject:
cytotoxicity; polymerization; ethyl ether; cations; ethylene glycol; transfection; genes; composite polymers; dextran
Abstract:
... It is of crucial importance to design reduction-sensitive polysaccharide-based copolymers for intracellular triggered gene and drug delivery. In this work, a simple two-step method involving the reaction of hydroxyl groups of dextran with cystamine was first developed to introduce reduction-sensitive disulfide linked initiation sites of atom transfer radical polymerization (ATRP) onto dextran. Wel ...
DOI:
10.1016/j.biomaterials.2011.11.027

31. Biocompatibility and degradation characteristics of PLGA-based electrospun nanofibrous scaffolds with nanoapatite incorporation

Author:
Wei Ji; Fang Yang; Hajar Seyednejad; Zhi Chen; Wim E. Hennink; James M. Anderson; Jeroen J.J.P. van den Beucken; John A. Jansen
Source:
Biomaterials 2012 v.33 no.28 pp. 6604-6614
ISSN:
0142-9612
Subject:
biocompatibility; cages; genes; giant cells; hybridization; macrophages; medical equipment; molecular weight; monocytes; polymers; rats; stainless steel; transforming growth factor beta; tumor necrosis factor-alpha
Abstract:
... The aim of current study was to evaluate the effect of nano-apatitic particles (nAp) incorporation on the degradation characteristics and biocompatibility of poly(lactide-co-glycolide) (PLGA)-based nanofibrous scaffolds. Composite PLGA/poly(ɛ-caprolactone) (PCL) blended (w/w = 3/1) polymeric electrospun scaffolds with 0–30 wt% of nAp incorporation (n0–n30) were prepared. The obtained scaffolds wer ...
DOI:
10.1016/j.biomaterials.2012.06.018

32. Biofabrication of stratified biofilm mimics for observation and control of bacterial signaling

Author:
Xiaolong Luo; Hsuan-Chen Wu; Chen-Yu Tsao; Yi Cheng; Jordan Betz; Gregory F. Payne; Gary W. Rubloff; William E. Bentley
Source:
Biomaterials 2012 v.33 no.20 pp. 5136-5143
ISSN:
0142-9612
Subject:
bacteria; biofabrication; biofilm; drugs; phenotype; quorum sensing
Abstract:
... Signaling between cells guides biological phenotype. Communications between individual cells, clusters of cells and populations exist in complex networks that, in sum, guide behavior. There are few experimental approaches that enable high content interrogation of individual and multicellular behaviors at length and time scales commensurate with the signal molecules and cells themselves. Here we pr ...
DOI:
10.1016/j.biomaterials.2012.03.037

33. Biofilm formation in Staphylococcus implant infections. A review of molecular mechanisms and implications for biofilm-resistant materials

Author:
Carla Renata Arciola; Davide Campoccia; Pietro Speziale; Lucio Montanaro; John William Costerton
Source:
Biomaterials 2012 v.33 no.26 pp. 5967-5982
ISSN:
0142-9612
Subject:
DNA; Staphylococcus; acids; antibiotics; biocides; biocompatible materials; biofilm; exopolysaccharides; macrophages; nanomaterials; neutrophils; orthopedics; proteins; risk; tissues
Abstract:
... Implant infections in orthopaedics, as well as in many other medical fields, are chiefly caused by staphylococci. The ability of growing within a biofilm enhances the chances of staphylococci to protect themselves from host defences, antibiotic therapies, and biocides. Advances in scientific knowledge on structural molecules (exopolysaccharide, proteins, teichoic acids, and the most recently descr ...
DOI:
10.1016/j.biomaterials.2012.05.031

34. Biomechanical and angiogenic properties of tissue-engineered rat trachea using genipin cross-linked decellularized tissue

Author:
Johannes Haag; Silvia Baiguera; Philipp Jungebluth; Daniel Barale; Costantino Del Gaudio; Francesca Castiglione; Alessandra Bianco; Camilla E. Comin; Domenico Ribatti; Paolo Macchiarini
Source:
Biomaterials 2012 v.33 no.3 pp. 780-789
ISSN:
0142-9612
Subject:
biomechanics; cross-linking reagents; crosslinking; humans; inflammation; mechanical properties; rats; swine; tissues
Abstract:
... In this study, the obtainment and characterization of decellularized rat tracheal grafts are described. The detergent-enzymatic method, already used to develop bioengineered pig and human trachea scaffolds, has been applied to rat tracheae in order to obtain airway grafts suitable to be used to improve our knowledge on the process of tissue-engineered airway transplantation and regeneration. The r ...
DOI:
10.1016/j.biomaterials.2011.10.008

35. Biomedical applications of amino acid-modified chitosans: A review

Author:
Luca Casettari; Driton Vllasaliu; Jenny K.W. Lam; Mahmoud Soliman; Lisbeth Illum
Source:
Biomaterials 2012 v.33 no.30 pp. 7565-7583
ISSN:
0142-9612
Subject:
amino acids; chitosan; derivatization; drugs; gene therapy; nucleic acids; primary amines; solubility; tissue engineering; transfection
Abstract:
... The presence of reactive primary amines in the backbone structure of chitosan, enables the derivatisation with different functional groups and thereby improving and expanding its properties, such as solubility and mucoadhesiveness, for biomedical applications. Such derivatives can be exploited with good results in a number of biomedical areas, including enhancement of nucleic acid transfection in ...
DOI:
10.1016/j.biomaterials.2012.06.104

36. Biomimetic hydration lubrication with various polyelectrolyte layers on cross-linked polyethylene orthopedic bearing materials

Author:
Masayuki Kyomoto; Toru Moro; Kenichi Saiga; Masami Hashimoto; Hideya Ito; Hiroshi Kawaguchi; Yoshio Takatori; Kazuhiko Ishihara
Source:
Biomaterials 2012 v.33 no.18 pp. 4451-4459
ISSN:
0142-9612
Subject:
crosslinking; orthopedics; longevity; prostheses; biomimetics; zwitterions; lubricants; cartilage; durability; hydrophilicity; polyethylene; hydrophilic polymers; inorganic ions
Abstract:
... Natural joints rely on fluid thin-film lubrication by the hydrated polyelectrolyte layer of cartilage. However, current artificial joints with polyethylene (PE) surfaces have considerably less efficient lubrication and thus much greater wear, leading to osteolysis and aseptic loosening. This is considered a common factor limiting prosthetic longevity in total hip arthroplasty (THA). However, such ...
DOI:
10.1016/j.biomaterials.2012.03.028

37. Bioreducible poly(amidoamine)s as carriers for intracellular protein delivery to intestinal cells

Author:
Shmuel Cohen; Grégory Coué; Delila Beno; Rafi Korenstein; Johan F.J. Engbersen
Source:
Biomaterials 2012 v.33 no.2 pp. 614-623
ISSN:
0142-9612
Subject:
cyclosporine; cytosol; drug resistance; endosomes; human serum albumin; intestines; lysosomes; mucus; nanoparticles; polymers; transporters
Abstract:
... An effective intracellular protein delivery system was developed based on linear poly(amidoamine)s (PAAs) that form self-assembled cationic nanocomplexes with oppositely charged proteins. Two differently functionalized PAAs were synthesized by Michael-type polyaddition of 4-amino-1-butanol (ABOL) to cystamine bisacrylamide (CBA) and to bisacryloylpiperazine (BAP), yielding p(CBA-ABOL) and p(BAP-AB ...
DOI:
10.1016/j.biomaterials.2011.09.085

38. Biscarbamate cross-linked low molecular weight PEI for delivering IL-1 receptor antagonist gene to synoviocytes for arthritis therapy

Author:
Shengnan Xiang; Jing Su; Haijun Tong; Fei Yang; Wenxue Tong; Weien Yuan; Fei Wu; Chuandong Wang; Tuo Jin; Kerong Dai; Xiaoling Zhang
Source:
Biomaterials 2012 v.33 no.27 pp. 6520-6532
ISSN:
0142-9612
Subject:
crosslinking; ethylene; anti-inflammatory activity; antagonists; cytotoxicity; DNA; metalloproteinases; arthritis; therapeutics; cartilage; transfection; genes; molecular weight; interleukin-1beta; apoptosis
Abstract:
... Cytoxicity is an essential concern for polyethyleneimine 25 kDa (PEI 25 kDa), a widely reported, highly effective transfection agent used in gene delivery. In our recent experiments, Small molecular weight cross-linked poly(ethylene imine) by biscarbamate linkage (PEI–Bu) (Mn: 3278, Mw: 4289) can reduce target cell apoptosis induced by polycationic transfection, and has almost the same DNA condens ...
DOI:
10.1016/j.biomaterials.2012.05.044

39. Blood vessel formation in the tissue-engineered bone with the constitutively active form of HIF-1α mediated BMSCs

Author:
Duohong Zou; Zhiyuan Zhang; Jiacai He; Kai Zhang; Dongxia Ye; Wei Han; Jian Zhou; Yuanyin Wang; Quanli Li; Xin Liu; Xin Zhang; Shaoyi Wang; Jingzhou Hu; Chao Zhu; Wenjie Zhang; Yong zhou; Honghai Fu; Yuanliang Huang; Xinquan Jiang
Source:
Biomaterials 2012 v.33 no.7 pp. 2097-2108
ISSN:
0142-9612
Subject:
angiogenesis; blood vessels; fibroblast growth factor 2; gelatin; gene overexpression; histology; messenger RNA; models; photography; rats; stem cell factor; stem cells; tissue engineering; vascular endothelial growth factors
Abstract:
... The successful clinical outcome of the implanted tissue-engineered bone is dependent on the establishment of a functional vascular network. A gene-enhanced tissue engineering represents a promising approach for vascularization. Our previous study indicated that hypoxia-inducible factor-1α (HIF-1α) can up-regulate the expression of vascular endothelial growth factor (VEGF) and stromal-derived facto ...
DOI:
10.1016/j.biomaterials.2011.11.053

40. Bridging peripheral nerve defects with a tissue engineered nerve graft composed of an in vitro cultured nerve equivalent and a silk fibroin-based scaffold

Author:
Xin Tang; Chengbin Xue; Yaxian Wang; Fei Ding; Yumin Yang; Xiaosong Gu
Source:
Biomaterials 2012 v.33 no.15 pp. 3860-3867
ISSN:
0142-9612
Subject:
Schwann cells; Western blotting; cell growth; coculture; ganglia; growth factors; nerve tissue; rats; silk
Abstract:
... Tissue engineered nerve grafts are considered as a promising alternative to autologous nerve grafts used for peripheral nerve repair. The differences between these two types of nerve grafts are mainly in the regenerative microenvironment established by them. To construct ideal tissue engineered nerve grafts, it is therefore required to develop a better way to introduce biochemical cues into a neur ...
DOI:
10.1016/j.biomaterials.2012.02.008

41. Bright blue emitting CuSe/ZnS/silica core/shell/shell quantum dots and their biocompatibility

Author:
Cherukaraveedu V. Durgadas; Kunnatheri Sreenivasan; Chandra P. Sharma
Source:
Biomaterials 2012 v.33 no.27 pp. 6420-6429
ISSN:
0142-9612
Subject:
apoptosis; biocompatibility; blood cells; coatings; drugs; neoplasm cells; quantum dots; reactive oxygen species; silica; toxicity
Abstract:
... In the in vivo scenario, the nanomaterials have to stay for a prolonged period in fluids like blood and therefore a detailed understanding of their interaction with blood elements is mandatory. We report the synthesis and characterization of highly blue emitting smaller size, 3.6 nm CuSe quantum dots (QDs) and its core/shell structure CuSe/ZnS. We modified QDs with a silica shell, which is the mos ...
DOI:
10.1016/j.biomaterials.2012.05.051

42. CD44 antibody-targeted liposomal nanoparticles for molecular imaging and therapy of hepatocellular carcinoma

Author:
Lina Wang; Weijun Su; Ze Liu; Manqian Zhou; Si Chen; Yanan Chen; Dan Lu; Yanhua Liu; Yan Fan; Yizhou Zheng; Zhongchao Han; Deling Kong; Joseph C. Wu; Rong Xiang; Zongjin Li
Source:
Biomaterials 2012 v.33 no.20 pp. 5107-5114
ISSN:
0142-9612
Subject:
bioluminescence; doxorubicin; gene therapy; green fluorescent protein; hepatoma; herpes simplex; human cell lines; image analysis; liver; luciferase; mice; models; monitoring; nanoparticles; plasmids; reporter genes; severe combined immunodeficiency; stem cells; thymidine kinase; viruses
Abstract:
... Most hepatocellular carcinoma (HCC) therapies fail to target cancer stem cells (CSCs) and monitor cancer progression or regression. The purpose of this study was to evaluate the possibility of cancer imaging and simultaneously monitoring targeted therapy in a single animal by anti-CD44 antibody-mediated liposomal nanoparticle. In this study, an in situ liver tumor model was applied for therapy by ...
DOI:
10.1016/j.biomaterials.2012.03.067

43. The CD44/integrins interplay and the significance of receptor binding and re-presentation in the uptake of RGD-functionalized hyaluronic acid

Author:
Sihem Ouasti; Paul J. Kingham; Giorgio Terenghi; Nicola Tirelli
Source:
Biomaterials 2012 v.33 no.4 pp. 1120-1134
ISSN:
0142-9612
Subject:
hyaluronic acid; integrins; macrophages; mice; models; molecular weight; receptors
Abstract:
... We have studied the interplay between two endocytic receptors for a carrier structure bearing two complementary ligands. Hyaluronic acid (HA; three different molecular weights) was functionalized with an RGD-containing peptide; this ancillary ligand allows the macromolecule to bind to αᵥ integrins in addition to the classical HA internalization receptor (CD44). The uptake of HA-RGD and of native H ...
DOI:
10.1016/j.biomaterials.2011.10.009

44. Cancer cell labeling and tracking using fluorescent and magnetic nanodiamond

Author:
Zhi-Yi Lien; Tzu-Chia Hsu; Kuang-Kai Liu; Wei-Siang Liao; Kuo-Chu Hwang; Jui-I. Chao
Source:
Biomaterials 2012 v.33 no.26 pp. 6172-6185
ISSN:
0142-9612
Subject:
bladder; carbon; cell viability; colon; cryopreservation; cytotoxicity; fibroblasts; flow cytometry; fluorescence; growth retardation; humans; lung neoplasms; magnetic properties; monitoring; nanodiamonds; neoplasm cells; protein synthesis
Abstract:
... Nanodiamond, a promising carbon nanomaterial, develops for biomedical applications such as cancer cell labeling and detection. Here, we establish the nanodiamond-bearing cancer cell lines using the fluorescent and magnetic nanodiamond (FMND). Treatment with FMND particles did not significantly induce cytotoxicity and growth inhibition in HFL-1 normal lung fibroblasts and A549 lung cancer cells. Th ...
DOI:
10.1016/j.biomaterials.2012.05.009

45. Cancer stem cell labeling using poly(l-lysine)-modified iron oxide nanoparticles

Author:
Xueqin Wang; Fang Wei; Ajing Liu; Lei Wang; Jian-Chun Wang; Li Ren; Wenming Liu; Qin Tu; Li Li; Jinyi Wang
Source:
Biomaterials 2012 v.33 no.14 pp. 3719-3732
ISSN:
0142-9612
Subject:
apoptosis; atomic absorption spectrometry; cell cycle; cell viability; flow cytometry; immunocytochemistry; iron oxides; lysine; monitoring; nanoparticles; propidium; reverse transcriptase polymerase chain reaction; stem cells; therapeutics
Abstract:
... Cell labeling using magnetic nanoparticles is an increasingly used approach in noninvasive behavior tracking, in vitro separation of cancer stem cells (CSCs), and CSC-based research in cancer therapy. However, the impact of magnetic labeling on the biological properties of targeted CSCs, such as self-renewal, proliferation, multi-differentiation, cell cycle, and apoptosis, remains elusive. The pre ...
DOI:
10.1016/j.biomaterials.2012.01.058

46. Cardiac differentiation of embryonic stem cells by substrate immobilization of insulin-like growth factor binding protein 4 with elastin-like polypeptides

Author:
Ayaka Minato; Hirohiko Ise; Mitsuaki Goto; Toshihiro Akaike
Source:
Biomaterials 2012 v.33 no.2 pp. 515-523
ISSN:
0142-9612
Subject:
binding proteins; cardiomyocytes; embryonic stem cells; medicine; polypeptides; polystyrenes; somatomedins; tissue engineering
Abstract:
... The establishment of cardiomyocyte differentiation of embryonic stem cells (ESCs) is a useful strategy for cardiovascular regenerative medicine. Here, we report a strategy for cardiomyocyte differentiation of ESCs using substrate immobilization of insulin-like growth factor binding protein 4 (IGFBP4) with elastin-like polypeptides. Recently, IGFBP4 was reported to promote cardiomyocyte differentia ...
DOI:
10.1016/j.biomaterials.2011.09.070

47. Cardiac repair achieved by bone marrow mesenchymal stem cells/silk fibroin/hyaluronic acid patches in a rat of myocardial infarction model

Author:
Nai-Hsin Chi; Ming-Chia Yang; Tze-Wen Chung; Jia-Yu Chen; Nai-Kuan Chou; Shoei-Shen Wang
Source:
Biomaterials 2012 v.33 no.22 pp. 5541-5551
ISSN:
0142-9612
Subject:
apoptosis; bioactive properties; bone marrow; echocardiography; hyaluronic acid; immune response; inflammation; models; myocardial infarction; quantitative polymerase chain reaction; rats; silk; stem cells; therapeutics; vascular endothelial growth factors; viability
Abstract:
... Bone marrow mesenchymal stem cells/silk fibroin/hyaluronic acid (BMSC/SH) patches were implanted into myocardial infarction (MI) rat hearts to investigate the efficacies of them on enhancing left ventricular (LV) remodeling and cardiac repair. 45 rats were divided into four groups: Sham, MI (MI hearts, induced by a cryo-injury technique), SH and BMSC/SH (MI hearts with implantations of SH and BMSC ...
DOI:
10.1016/j.biomaterials.2012.04.030

48. Cardiac tissue engineering using tissue printing technology and human cardiac progenitor cells

Author:
Roberto Gaetani; Peter A. Doevendans; Corina H.G. Metz; Jacqueline Alblas; Elisa Messina; Alessandro Giacomello; Joost P.G. Sluijter
Source:
Biomaterials 2012 v.33 no.6 pp. 1782-1790
ISSN:
0142-9612
Subject:
biocompatible materials; bioprinting; cardiomyocytes; cell viability; functional properties; gene expression; humans; stem cells; therapeutics; tissue engineering; transcription factors
Abstract:
... Tissue engineering is emerging as a potential therapeutic approach to overcome limitations of cell therapy, like cell retention and survival, as well as to mechanically support the ventricular wall and thereby prevent dilation. Tissue printing technology (TP) offers the possibility to deliver, in a defined and organized manner, scaffolding materials and living cells. The aim of our study was to ev ...
DOI:
10.1016/j.biomaterials.2011.11.003

49. Cartilage regeneration in SCID mice using a highly organized three-dimensional alginate scaffold

Author:
Chen-Chie Wang; Kai-Chiang Yang; Keng-Hui Lin; Yen-Liang Liu; Hwa-Chang Liu; Feng-Huei Lin
Source:
Biomaterials 2012 v.33 no.1 pp. 120-127
ISSN:
0142-9612
Subject:
cartilage; chondrocytes; collagen; gene expression regulation; glycosaminoglycans; immunohistochemistry; in vitro culture; messenger RNA; mice; osteoarthritis; phenotype; quantitative polymerase chain reaction; severe combined immunodeficiency; surgery; swine; tissue engineering
Abstract:
... Tissue engineering for cartilage regeneration provides an alternative to surgery for degenerative osteoarthritis. Recently, a highly organized three-dimensional (3D) alginate scaffold was prepared using a microfluidic device; this scaffold is effective for chondrocyte culture in vitro. The performance of this scaffold was further demonstrated; an alginate scaffold seeded with porcine chondrocytes ...
DOI:
10.1016/j.biomaterials.2011.09.042

50. Cartilage repair in transplanted scaffold-free chondrocyte sheets using a minipig model

Author:
Goro Ebihara; Masato Sato; Masayuki Yamato; Genya Mitani; Toshiharu Kutsuna; Toshihiro Nagai; Satoshi Ito; Taku Ukai; Miyuki Kobayashi; Mami Kokubo; Teruo Okano; Joji Mochida
Source:
Biomaterials 2012 v.33 no.15 pp. 3846-3851
ISSN:
0142-9612
Subject:
animal models; blood; cartilage; miniature swine; rabbits; tissue engineering; tissue repair
Abstract:
... Lacking a blood supply and having a low cellular density, articular cartilage has a minimal ability for self-repair. Therefore, wide-ranging cartilage damage rarely resolves spontaneously. Cartilage damage is typically treated by chondrocyte transplantation, mosaicplasty or microfracture. Recent advances in tissue engineering have prompted research on techniques to repair articular cartilage damag ...
DOI:
10.1016/j.biomaterials.2012.01.056