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- Author:
- Christopher S. Szot; Cara F. Buchanan; Joseph W. Freeman; Marissa N. Rylander
- Source:
- Biomaterials 2011 v.32 no.31 pp. 7905-7912
- ISSN:
- 0142-9612
- Subject:
- biocompatibility; breast neoplasms; cell proliferation; collagen; cultured cells; gene expression; humans; hydrocolloids; hypoxia; models; necrosis; neoplasm cells; nutrient availability; oxygen; phenotype; polystyrenes; tissue culture; tissue engineering; vascular endothelial growth factor A
- Abstract:
- ... Cells cultured within a three-dimensional (3D) in vitro environment have the ability to acquire phenotypes and respond to stimuli analogous to in vivo biological systems. This approach has been utilized in tissue engineering and can also be applied to the development of a physiologically relevant in vitro tumor model. In this study, collagen I hydrogels cultured with MDA-MB-231 human breast cancer ...
- DOI:
- 10.1016/j.biomaterials.2011.07.001
- PubMed:
- 21782234
- PubMed Central:
- PMC3229258
- https://doi.org/10.1016/j.biomaterials.2011.07.001
- Author:
- Halime Kenar; Gamze T. Kose; Mehmet Toner; David L. Kaplan; Vasif Hasirci
- Source:
- Biomaterials 2011 v.32 no.23 pp. 5320-5329
- ISSN:
- 0142-9612
- Subject:
- biodegradability; cell viability; culture media; fluorescence; fluorescence microscopy; glycerol; humans; myocardial infarction; myocardium; polyesters; stem cells; umbilical cord
- Abstract:
- ... The goal of this study was to design and develop a myocardial patch to use in the repair of myocardial infarctions or to slow down tissue damage and improve long-term heart function. The basic 3D construct design involved two biodegradable macroporous tubes, to allow transport of growth media to the cells within the construct, and cell seeded, aligned fiber mats wrapped around them. The microfibro ...
- DOI:
- 10.1016/j.biomaterials.2011.04.025
- PubMed:
- 21570112
- PubMed Central:
- PMC4492442
- https://doi.org/10.1016/j.biomaterials.2011.04.025
- Author:
- Lin Sun; Xiuli Wang; David L. Kaplan
- Source:
- Biomaterials 2011 v.32 no.24 pp. 5581-5589
- ISSN:
- 0142-9612
- Subject:
- animal models; apoptosis; cartilage; caspase-3; cell culture; cell viability; chondrocytes; collagen; early development; fibroblasts; gene expression; gene expression regulation; genes; humans; hypertrophy; immunohistochemistry; in vitro studies; inflammation; interleukin-1beta; macrophages; osteoarthritis; reverse transcriptase polymerase chain reaction; silk proteins; tumor necrosis factor-alpha
- Abstract:
- ... Inflammation plays a major role in the destruction of cartilage in osteoarthritis (OA), with the interaction of multiple mediators, immune cells, fibroblasts and chondrocytes. Current 2D studies in vitro with cell lines, as well as animal models, are limited in terms of providing insight into pathogenic mechanisms related to the human system. Hence, an in vitro human 3D cartilage tissue system was ...
- DOI:
- 10.1016/j.biomaterials.2011.04.028
- PubMed:
- 21565399
- PubMed Central:
- PMC3109142
- https://doi.org/10.1016/j.biomaterials.2011.04.028
- Author:
- Lesley W. Chow; Ronit Bitton; Matthew J. Webber; Daniel Carvajal; Kenneth R. Shull; Arun K. Sharma; Samuel I. Stupp
- Source:
- Biomaterials 2011 v.32 no.6 pp. 1574-1582
- ISSN:
- 0142-9612
- Subject:
- angiogenesis; binding capacity; bioactive properties; chickens; chorioallantoic membrane; fibroblast growth factor 2; heparin; humans; hyaluronic acid; stem cells; vascular endothelial growth factors
- Abstract:
- ... We report here on a bioactive hierarchically structured membrane formed by self-assembly. The membrane is formed with hyaluronic acid and peptide amphiphiles with binding affinity for heparin, and its hierarchical structure contains both an amorphous zone and a layer of fibrils oriented perpendicular to the membrane plane. The design of bioactivity is based on the potential ability to bind and slo ...
- DOI:
- 10.1016/j.biomaterials.2010.10.048
- PubMed:
- 21093042
- PubMed Central:
- PMC3150553
- https://doi.org/10.1016/j.biomaterials.2010.10.048
- Author:
- Eva-Maria Engelhardt; Lionel A. Micol; Stephanie Houis; Florian M. Wurm; Jöns Hilborn; Jeffrey A. Hubbell; Peter Frey
- Source:
- Biomaterials 2011 v.32 no.16 pp. 3969-3976
- ISSN:
- 0142-9612
- Subject:
- bladder; collagen; crosslinking; cultured cells; electron microscopy; gels; humans; hybrids; immunohistochemistry; mechanical properties; mice; polymers; smooth muscle; sowing; tissue repair
- Abstract:
- ... Scaffold materials should favor cell attachment and proliferation, and provide designable 3D structures with appropriate mechanical strength. Collagen matrices have proven to be beneficial scaffolds for tissue regeneration. However, apart from small intestinal submucosa, they offer a limited mechanical strength even if crosslinking can enhance their mechanical properties. A more cell-friendly way ...
- DOI:
- 10.1016/j.biomaterials.2011.02.012
- https://doi.org/10.1016/j.biomaterials.2011.02.012
- Author:
- Manhal Habib; Keren Shapira-Schweitzer; Oren Caspi; Amira Gepstein; Gil Arbel; Doron Aronson; Dror Seliktar; Lior Gepstein
- Source:
- Biomaterials 2011 v.32 no.30 pp. 7514-7523
- ISSN:
- 0142-9612
- Subject:
- humans; biopolymers; hydrocolloids; therapeutics; cardiomyocytes; biodegradability; rats; echocardiography
- Abstract:
- ... Myocardial cell-replacement strategies are hampered by limited sources for human cardiomyocytes and by significant cell loss following transplantation. We tested the hypothesis that a combined delivery of cardiomyocytes with an in-situ polymerizable hydrogel into a post-MI rat heart will result in better functional outcomes than each intervention alone. A photopolymerizable, biodegradable, PEGylat ...
- DOI:
- 10.1016/j.biomaterials.2011.06.049
- https://doi.org/10.1016/j.biomaterials.2011.06.049
- Author:
- Margit Kempf; Yuki Miyamura; Pei-Yun Liu; Alice C.-H. Chen; Hideki Nakamura; Hiroshi Shimizu; Yasuhiko Tabata; Roy M. Kimble; James R. McMillan
- Source:
- Biomaterials 2011 v.32 no.21 pp. 4782-4792
- ISSN:
- 0142-9612
- Subject:
- adhesion; adsorption; biocompatibility; biodegradation; blood proteins; cattle; coculture; collagen; desmosomes; encapsulation; fibroblasts; humans; immune response; islands; keratinocytes; mice; polymers; severe combined immunodeficiency; skin grafting; transmission electron microscopy
- Abstract:
- ... Biomaterial scaffolds are categorized into artificial or natural polymers, or combinations of the two. Artificial polymers often undergo serum protein adsorption, elicit foreign body and encapsulation immune responses post-implantation. Large pore bovine electrospun collagen I was therefore screened as a candidate for human keratinocyte and fibroblast cell scaffolds. Human HaCaT keratinocyte and d ...
- DOI:
- 10.1016/j.biomaterials.2011.03.023
- https://doi.org/10.1016/j.biomaterials.2011.03.023
- Author:
- Yi-An Chen; Hsiao-Che Kuo; Young-Mao Chen; Shin-Yi Huang; Yu-Ru Liu; Su-Ching Lin; Huey-Lang Yang; Tzong-Yueh Chen
- Source:
- Biomaterials 2011 v.32 no.17 pp. 4174-4184
- ISSN:
- 0142-9612
- Subject:
- Cercopithecus aethiops; DNA topoisomerase; Escherichia coli; adenosine triphosphate; amino acids; cytotoxicity; genes; grouper; humans; kidney cells; nucleotide sequences; plasmids; purification methods; topology; transfection
- Abstract:
- ... The N-terminal 200 amino acid residues of topoisomerase I (TopoN) is highly positive in charge and has DNA binding activity, without DNA sequence and topological specificity. Here, a fusion protein (6 × His-PTD-TopoN) containing a hexahistidine (6 × His) tag, a membrane penetration domain and TopoN (amino acid 3–200) was designed and developed. The protein can bind to different sizes (3.0–8.0 kb) ...
- DOI:
- 10.1016/j.biomaterials.2011.02.041
- https://doi.org/10.1016/j.biomaterials.2011.02.041
- Author:
- Cheng-Yi Lee; Jheng-Fong Li; Ji-Sing Liou; Yuh-Chyang Charng; Yue-Wern Huang; Han-Jung Lee
- Source:
- Biomaterials 2011 v.32 no.26 pp. 6264-6276
- ISSN:
- 0142-9612
- Subject:
- amino acids; excision; gene therapy; genomics; green fluorescent protein; humans; peptides; plasmids; promoter regions; reporter genes; transposition (genetics); transposons
- Abstract:
- ... The piggyBac (PB) transposable element has recently accumulated enormous attention as a tool for the transgenesis in various eukaryotic organisms. Arginine-rich cell-penetrating peptides (CPPs) are protein transduction domains containing a large amount of basic amino acids that were found to be capable of delivering biologically active macromolecules into living cells. In this study, we demonstrat ...
- DOI:
- 10.1016/j.biomaterials.2011.05.012
- https://doi.org/10.1016/j.biomaterials.2011.05.012
- Author:
- Maiqin Chen; Xiu Wang; Zhaoyang Ye; Yan Zhang; Yan Zhou; Wen-Song Tan
- Source:
- Biomaterials 2011 v.32 no.30 pp. 7532-7542
- ISSN:
- 0142-9612
- Subject:
- adults; alizarin; bone formation; calcium; cell viability; collagen; gelatin; gene expression; humans; mass transfer; osteocalcin; stem cells; swine; tissue engineering; tissues
- Abstract:
- ... Tissue engineering of clinical-relevant large tissue constructs remains a big challenge due to the mass transfer limit. A modular approach via the assembling of modular tissues thus eliminating the mass transfer limit holds great promise for fabricating centimeter-sized constructs. In the present study, we investigated the feasibility of using microcarriers seeded with adult mesenchymal stem cells ...
- DOI:
- 10.1016/j.biomaterials.2011.06.054
- https://doi.org/10.1016/j.biomaterials.2011.06.054
- Author:
- Margaret D.M. Evans; Ruby K. Prakasam; Pravin K. Vaddavalli; Timothy C. Hughes; Warren Knower; John S. Wilkie; Keith M. McLean; Graham Johnson; Gail A. McFarland; Ruo Zhong Xie; Deborah F. Sweeney
- Source:
- Biomaterials 2011 v.32 no.12 pp. 3158-3165
- ISSN:
- 0142-9612
- Subject:
- complications (disease); cornea; humans; polymers; rabbits
- Abstract:
- ... This study assessed the long-term biological response of a perfluoropolyether-based polymer developed as a corneal inlay to correct refractive error. The polymer formulation met chemical and physical specifications and was non-cytotoxic when tested using standard in vitro techniques. It was cast into small microporous membranes that were implanted as inlays into corneas of rabbits (n = 5) and unsi ...
- DOI:
- 10.1016/j.biomaterials.2011.01.047
- https://doi.org/10.1016/j.biomaterials.2011.01.047
- Author:
- Chuncai Zhou; Peng Li; Xiaobao Qi; Abdul Rahim Mohamed Sharif; Yin Fun Poon; Ye Cao; Matthew W. Chang; Susanna Su Jan Leong; Mary B. Chan-Park
- Source:
- Biomaterials 2011 v.32 no.11 pp. 2704-2712
- ISSN:
- 0142-9612
- Subject:
- Candida albicans; Escherichia coli; Fusarium solani; Pseudomonas aeruginosa; Serratia marcescens; Staphylococcus aureus; anti-infective properties; bacteria; biocompatibility; coatings; erythrocytes; fungi; hemolysis; humans; hydrocolloids; medical equipment
- Abstract:
- ... Hydrogels made from epsilon-poly-l-lysine-graft-methacrylamide (EPL-MA) have been found to have impressive wide spectrum antimicrobial activity against both bacteria (specifically Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens and Staphylococcus aureus) and fungi (specifically Candida albicans and Fusarium solani). The EPL-MA hydrogel also possesses in vitro biocompatibility and EPL ...
- DOI:
- 10.1016/j.biomaterials.2010.12.040
- https://doi.org/10.1016/j.biomaterials.2010.12.040
- Author:
- Moonhee Kim; Miyoung Kim; Hyo Young Kim; Sol Kim; Jaehoon Jung; Jeehye Maeng; Jun Chang; Kyunglim Lee
- Source:
- Biomaterials 2011 v.32 no.1 pp. 222-230
- ISSN:
- 0142-9612
- Subject:
- Adenoviridae; beta-galactosidase; cations; cell membranes; cell nucleus; drugs; endocytosis; gene expression; gene expression regulation; glycoproteins; heart; humans; immune response; in vitro studies; in vivo studies; injection; intranasal administration; kidneys; lipids; liver; lungs; lysosomes; mice; neoplasms; oligopeptides; protein synthesis; respiratory tract diseases; spleen; translation (genetics); viruses
- Abstract:
- ... Protein transduction domains (PTDs) are small peptides, able to penetrate biological membranes and deliver various types of cargo both in vitro and in vivo. Because use of PTDs originating from viral origins resulted in undesired effects, PTDs originating from non-viral origins are needed. Here, we report that a 10-amino acid peptide (MIIYRDLISH) derived from the NH₂-terminus of human translationa ...
- DOI:
- 10.1016/j.biomaterials.2010.08.077
- https://doi.org/10.1016/j.biomaterials.2010.08.077
- Author:
- Yongkang Jiang; Hongwei Liu; Hong Li; Fangjun Wang; Kai Cheng; Guangdong Zhou; Wenjie Zhang; Mingliang Ye; Yinlin Cao; Wei Liu; Hanfa Zou
- Source:
- Biomaterials 2011 v.32 no.17 pp. 4085-4095
- ISSN:
- 0142-9612
- Subject:
- bioreactors; collagen; cytoskeleton; engineering; extracellular matrix; gene expression regulation; humans; inflammation; mass spectrometry; mechanotransduction; molecular weight; protein synthesis; proteomics; tendons; texture
- Abstract:
- ... Previous studies have demonstrated the beneficial effect of mechanical loading on in vitro tendon engineering. To understand the mechanism, human tenocytes and polyglycolic acid long fibers were used for in vitro tendon engineering in a bioreactor system for 12 weeks with and without dynamic loading. The engineered neo-tendons were subjected to proteomic analysis using mass spectrometry along with ...
- DOI:
- 10.1016/j.biomaterials.2011.02.033
- https://doi.org/10.1016/j.biomaterials.2011.02.033
- Author:
- Alexander Hoppe; Nusret S. Güldal; Aldo R. Boccaccini
- Source:
- Biomaterials 2011 v.32 no.11 pp. 2757-2774
- ISSN:
- 0142-9612
- Subject:
- angiogenesis; bioactive properties; biocompatible materials; bone formation; calcium phosphates; chemical composition; copper; endothelial cells; humans; magnesium; mechanical properties; porosity; stem cells; strontium; tissue engineering; topography; zinc
- Abstract:
- ... Several inorganic materials such as special compositions of silicate glasses, glass-ceramics and calcium phosphates have been shown to be bioactive and resorbable and to exhibit appropriate mechanical properties which make them suitable for bone tissue engineering applications. However, the exact mechanism of interaction between the ionic dissolution products of such inorganic materials and human ...
- DOI:
- 10.1016/j.biomaterials.2011.01.004
- https://doi.org/10.1016/j.biomaterials.2011.01.004
- Author:
- Jinhui Wu; Ian Wheeldon; Yuqi Guo; Tingli Lu; Yanan Du; Ben Wang; Jiankang He; Yiqiao Hu; Ali Khademhosseini
- Source:
- Biomaterials 2011 v.32 no.3 pp. 841-848
- ISSN:
- 0142-9612
- Subject:
- bioactive properties; breast neoplasms; cell culture; cell viability; cross contamination; fluorescence; humans; microarray technology; neoplasm cells; screening; toxicology; verapamil
- Abstract:
- ... The emergence of combinatorial chemistries and the increased discovery of natural compounds have led to the production of expansive libraries of drug candidates and vast numbers of compounds with potentially interesting biological activities. Despite broad interest in high throughput screening (HTS) across varied fields of biological research, there has not been an increase in accessible HTS techn ...
- DOI:
- 10.1016/j.biomaterials.2010.09.026
- PubMed:
- 20965560
- PubMed Central:
- PMC2991467
- https://doi.org/10.1016/j.biomaterials.2010.09.026
- Author:
- Takeaki Kawashima; Nobuhiro Nagai; Hirokazu Kaji; Norihiro Kumasaka; Hideyuki Onami; Yumi Ishikawa; Noriko Osumi; Matsuhiko Nishizawa; Toshiaki Abe
- Source:
- Biomaterials 2011 v.32 no.7 pp. 1950-1956
- ISSN:
- 0142-9612
- Subject:
- bioactive properties; collagen; drug formulations; drugs; epithelium; humans; polyethylene glycol; rabbits; retina; surgery
- Abstract:
- ... A transscleral drug-delivery device, designed for the administration of protein-type drugs, that consists of a drug reservoir covered with a controlled-release membrane was manufactured and tested. The controlled-release membrane is made of photopolymerized polyethylene glycol dimethacrylate (PEGDM) that contains interconnected collagen microparticles (COLs), which are the routes for drug permeati ...
- DOI:
- 10.1016/j.biomaterials.2010.11.006
- https://doi.org/10.1016/j.biomaterials.2010.11.006
- Author:
- Matthew B. Dowling; Rakesh Kumar; Mark A. Keibler; John R. Hess; Grant V. Bochicchio; Srinivasa R. Raghavan
- Source:
- Biomaterials 2011 v.32 no.13 pp. 3351-3357
- ISSN:
- 0142-9612
- Subject:
- alpha-cyclodextrin; animal models; biopolymers; blood cells; chitosan; fibrin; gelation; gels; hemorrhage; hemostasis; humans; hydrophobicity
- Abstract:
- ... Blood loss at the site of a wound in mammals is curtailed by the rapid formation of a hemostatic plug, i.e., a self-assembled network of the protein, fibrin that locally transforms liquid blood into a gelled clot. Here, we report an amphiphilic biopolymer that exhibits a similar ability to rapidly gel blood; moreover, the self-assembly underlying the gelation readily allows for reversibility back ...
- DOI:
- 10.1016/j.biomaterials.2010.12.033
- https://doi.org/10.1016/j.biomaterials.2010.12.033
- Author:
- Haijun Zhang; Baoan Chen; Hui Jiang; Cailian Wang; Huangping Wang; Xuemei Wang
- Source:
- Biomaterials 2011 v.32 no.7 pp. 1906-1914
- ISSN:
- 0142-9612
- Subject:
- air; anticarcinogenic activity; daunorubicin; drug carriers; drug therapy; flow cytometry; humans; immunocytochemistry; lighting; nanorods; neoplasm cells; reactive oxygen species; signal transduction; temperature; zinc oxide
- Abstract:
- ... Combinations of cancer therapy modalities are attracting attention to improve the outcome of treatment, since single modality has not always been sufficiently effective. The aim of this study was to investigate a new strategy of combined application of ZnO nanorods with anticancer drug daunorubicin (DNR) in photodynamic therapy (PDT). Using a simple one-step solid state reaction in air at room tem ...
- DOI:
- 10.1016/j.biomaterials.2010.11.027
- https://doi.org/10.1016/j.biomaterials.2010.11.027
- Author:
- Yang Liu; Xintong Wang; Dan S. Kaufman; Wei Shen
- Source:
- Biomaterials 2011 v.32 no.32 pp. 8058-8066
- ISSN:
- 0142-9612
- Subject:
- biomimetics; culture media; embryonic stem cells; flow cytometry; humans; integrins; reverse transcriptase polymerase chain reaction
- Abstract:
- ... Our ability to guide differentiation of human pluripotent stem cells (hPSCs) toward desired lineages efficiently and reproducibly in xeno-free conditions is the key to advancing hPSC technology from the laboratory to clinical use. Here we report an engineered biomimetic substrate functionalized with both peptide ligands for α5β1 and α6β1 integrins to support efficient early mesodermal differentiat ...
- DOI:
- 10.1016/j.biomaterials.2011.07.052
- PubMed:
- 21821284
- PubMed Central:
- PMC3189998
- https://doi.org/10.1016/j.biomaterials.2011.07.052
- Author:
- Sung-Hwan Moon; Jong Soo Kim; Soon-Jung Park; Hye Jin Lee; Jeong Tae Do; Hyung-Min Chung
- Source:
- Biomaterials 2011 v.32 no.27 pp. 6445-6455
- ISSN:
- 0142-9612
- Subject:
- adverse effects; animal models; bioavailability; carcinogenesis; embryonic stem cells; encapsulation; endothelial cells; humans; ischemia; medicine; therapeutics
- Abstract:
- ... Despite studies on the use of human embryonic stem cell (hESC) derivatives to treat ischemic diseases, there are technical safety issues that have yet to be resolved. Herein, we sought to develop a method for using secreted angiogenic factors from hESC-derived endothelial cell derivatives (hESC-ECs), while avoiding direct cell incorporation, to reduce tumorigenesis or unidentified side effects of ...
- DOI:
- 10.1016/j.biomaterials.2011.05.026
- https://doi.org/10.1016/j.biomaterials.2011.05.026
22. Activation of stress-regulated transcription factors by triethylene glycol dimethacrylate monomer
- Author:
- Stephanie Krifka; Christine Petzel; Carola Bolay; Karl-Anton Hiller; Gianrico Spagnuolo; Gottfried Schmalz; Helmut Schweikl
- Source:
- Biomaterials 2011 v.32 no.7 pp. 1787-1795
- ISSN:
- 0142-9612
- Subject:
- DNA damage; reactive oxygen species; signal transduction; Western blotting; genotoxicity; phosphorylation; mitogen-activated protein kinase; linear models; macrophages; cytoplasm; tissues; apoptosis; transcription factors; mineralization; humans; cell culture; polymerization; cell nucleus; composite materials; innate immunity; therapeutics; triethylene glycol; cell cycle
- Abstract:
- ... Triethylene glycol dimethacrylate (TEGDMA) is a resin monomer available for short exposure scenarios of oral tissues due to incomplete polymerization processes of dental composite materials. The generation of reactive oxygen species (ROS) in the presence of resin monomers is discussed as a common mechanism underlying cellular reactions as diverse as disturbed responses of the innate immune system, ...
- DOI:
- 10.1016/j.biomaterials.2010.11.031
- https://doi.org/10.1016/j.biomaterials.2010.11.031
- Author:
- Jinhao Gao; Kai Chen; Zheng Miao; Gang Ren; Xiaoyuan Chen; Sanjiv S. Gambhir; Zhen Cheng
- Source:
- Biomaterials 2011 v.32 no.8 pp. 2141-2148
- ISSN:
- 0142-9612
- Subject:
- cysteine; epidermal growth factor receptors; humans; image analysis; in vivo studies; iron oxides; nanoparticles; neoplasm cells; neoplasms; quantum dots
- Abstract:
- ... This article reports the affibody-based nanoprobes specifically target and image human epidermal growth factor receptor type 2 (HER2)-expressing cells and tumors. The affibody molecules are a promising class of targeting ligands with simple, robust, and precise structure and high affinity. Using near-infrared (NIR) quantum dots (QDs) and iron oxide (IO) nanoparticles as two representative nanomate ...
- DOI:
- 10.1016/j.biomaterials.2010.11.053
- PubMed:
- 21147502
- PubMed Central:
- PMC3032351
- https://doi.org/10.1016/j.biomaterials.2010.11.053
- Author:
- Jaemoon Yang; Eun-Sook Lee; Min-Young Noh; Seong-Ho Koh; Eun-Kyung Lim; A-Rum Yoo; Kwangyeol Lee; Jin-Suck Suh; Seung Hyun Kim; Seungjoo Haam; Yong-Min Huh
- Source:
- Biomaterials 2011 v.32 no.26 pp. 6174-6182
- ISSN:
- 0142-9612
- Subject:
- cell movement; gene expression; genes; humans; image analysis; models; monitoring; nanoparticles; polymers; rats; secretion; solvents; stem cells; stroke; transfection
- Abstract:
- ... The synchronization of gene expression and cell trafficking in transfected stem cells is crucial for augmentation of stem cell functions (differentiation and neurotropic factor secretion) and real time in vivo monitoring. We report a magnetic nanoparticle-based gene delivery system that can ensure simultaneous gene delivery and in vivo cell trafficking by high resolution MR imaging. The polar apro ...
- DOI:
- 10.1016/j.biomaterials.2011.04.007
- https://doi.org/10.1016/j.biomaterials.2011.04.007
- Author:
- Teodelinda Mirabella; Michele Cilli; Sebastiano Carlone; Ranieri Cancedda; Chiara Gentili
- Source:
- Biomaterials 2011 v.32 no.15 pp. 3689-3699
- ISSN:
- 0142-9612
- Subject:
- amniotic fluid; arteries; endothelial cells; endothelium; engineering; humans; interleukin-8; medicine; mice; models; muscles; necrosis; phenotype; stem cells; tissue repair; vascular endothelial growth factors
- Abstract:
- ... Most urgent health problems are related to a blood vessel formation failure. The use of stem cells from different sources or species for both in vitro and in vivo engineering of endothelium does not necessarily imply their direct commitment towards a vascular phenotype. In the present study, we used human amniotic fluid stem cells (AFSC) to evoke a strong angiogenic response in murine recipients, ...
- DOI:
- 10.1016/j.biomaterials.2011.01.071
- https://doi.org/10.1016/j.biomaterials.2011.01.071
- Author:
- Shi-zhao Ji; Shi-chu Xiao; Peng-fei Luo; Guo-feng Huang; Guang-yi Wang; Shi-hui Zhu; Min-juan Wu; Zhao-fan Xia
- Source:
- Biomaterials 2011 v.32 no.31 pp. 7801-7811
- ISSN:
- 0142-9612
- Subject:
- active ingredients; basement membrane; bioactive properties; cell culture; cell differentiation; cell viability; collagen; humans; mice; nitrogen; stem cells; tissue engineering; transforming growth factor beta 1
- Abstract:
- ... How to amplify epidermal stem cells (ESCs) rapidly is a challenging crux in skin tissue engineering research. The present study describes the preparation of 3D micronized (300–600 μm) amniotic membrane (mAM) by means of repeated freeze-thawing cycles to deplete cell components and homogenized with a macrohomogenizer in liquid nitrogen. This newly prepared mAM not only possessed the characteristics ...
- DOI:
- 10.1016/j.biomaterials.2011.06.076
- https://doi.org/10.1016/j.biomaterials.2011.06.076
- Author:
- Christopher D. Pritchard; Timothy M. O’Shea; Daniel J. Siegwart; Eliezer Calo; Daniel G. Anderson; Francis M. Reynolds; John A. Thomas; Jonathan R. Slotkin; Eric J. Woodard; Robert Langer
- Source:
- Biomaterials 2011 v.32 no.2 pp. 587-597
- ISSN:
- 0142-9612
- Subject:
- biocompatible materials; ethylene glycol; gelation; humans; hydrocolloids; mechanical properties; mice; modulus of elasticity; sodium; stem cells; succinic acid; thiols; toxicity
- Abstract:
- ... Clinically available injectable hydrogels face technical challenges associated with swelling after injection and toxicity from unreacted constituents that impede their performance as surgical biomaterials. To overcome these challenges, we developed a system where chemical gelation was controlled by a conjugate Michael addition between thiol and acrylate in aqueous media, with 97% monomer conversio ...
- DOI:
- 10.1016/j.biomaterials.2010.08.106
- PubMed:
- 20880573
- PubMed Central:
- PMC4070531
- https://doi.org/10.1016/j.biomaterials.2010.08.106
28. Angiogenesis in ischemic tissue produced by spheroid grafting of human adipose-derived stromal cells
- Author:
- Suk Ho Bhang; Seung-Woo Cho; Wan-Geun La; Tae-Jin Lee; Hee Seok Yang; Ah-Young Sun; Sang-Hong Baek; Jong-Won Rhie; Byung-Soo Kim
- Source:
- Biomaterials 2011 v.32 no.11 pp. 2734-2747
- ISSN:
- 0142-9612
- Subject:
- angiogenesis; apoptosis; cell harvesting; cell transplantation; cell viability; chemokine CXCL12; enzymatic treatment; fibroblast growth factor 2; genetic engineering; hepatocyte growth factor; hindlimbs; humans; ischemia; mice; proteolysis; secretion; stem cells; stromal cells; vascular endothelial growth factors
- Abstract:
- ... Stem cells offer significant therapeutic promise for the treatment of ischemic disease. However, stem cells transplanted into ischemic tissue exhibit limited therapeutic efficacy due to poor engraftment in vivo. Several strategies for improving the survival and engraftment of stem cells in ischemic tissue have been developed including transplantation in combination with growth factor delivery, gen ...
- DOI:
- 10.1016/j.biomaterials.2010.12.035
- https://doi.org/10.1016/j.biomaterials.2010.12.035
- Author:
- Hironobu Takahashi; Masamichi Nakayama; Tatsuya Shimizu; Masayuki Yamato; Teruo Okano
- Source:
- Biomaterials 2011 v.32 no.34 pp. 8830-8838
- ISSN:
- 0142-9612
- Subject:
- actin; biomimetics; collagen; extracellular matrix; fibroblasts; fluorescence; humans; image analysis; medicine; temperature; transforming growth factor beta 1; vascular endothelial growth factors
- Abstract:
- ... Normal human dermal fibroblasts were aligned on micropatterned thermoresponsive surfaces simply by one-pot cell seeding. After they proliferated with maintaining their orientation, anisotropic cell sheets were harvested by reducing temperature to 20 °C. Surprisingly, the cell sheets showed different shrinking rates between vertical and parallel sides of the cell alignment (aspect ratio: approx. 3: ...
- DOI:
- 10.1016/j.biomaterials.2011.08.006
- https://doi.org/10.1016/j.biomaterials.2011.08.006
- Author:
- Hengyi Xu; Zoraida P. Aguilar; Lily Yang; Min Kuang; Hongwei Duan; Yonghua Xiong; Hua Wei; Andrew Wang
- Source:
- Biomaterials 2011 v.32 no.36 pp. 9758-9765
- ISSN:
- 0142-9612
- Subject:
- antibodies; blood; breast neoplasms; humans; immunomagnetic separation; iron oxides; magnetic fields; membrane proteins; nanoparticles; neoplasm cells; polymers
- Abstract:
- ... A highly efficient process using iron oxide magnetic nanoparticles (IO)-based immunomagnetic separation of tumor cells from fresh whole blood has been developed. The process involved polymer coated 30 nm IO that was modified with antibodies (Ab) against human epithelial growth factor receptor 2 (anti-HER2 or anti-HER2/neu) forming IO-Ab. HER2 is a cell membrane protein that is overexpressed in sev ...
- DOI:
- 10.1016/j.biomaterials.2011.08.076
- PubMed:
- 21920599
- PubMed Central:
- PMC3220927
- https://doi.org/10.1016/j.biomaterials.2011.08.076
- Author:
- Sílvia C. Gomes; Isabel B. Leonor; João F. Mano; Rui L. Reis; David L. Kaplan
- Source:
- Biomaterials 2011 v.32 no.18 pp. 4255-4266
- ISSN:
- 0142-9612
- Subject:
- Araneae; Fourier transform infrared spectroscopy; Staphylococcus aureus; anti-infective properties; biocompatible materials; bioengineering; chimerism; consensus sequence; crosslinking; hepcidin; humans; light scattering; neutrophils; nucleotide sequences; osteosarcoma; recombinant DNA; recombinant proteins; reflectance; silk; silk proteins
- Abstract:
- ... Genetically engineered fusion proteins offer potential as multifunctional biomaterials for medical use. Fusion or chimeric proteins can be formed using recombinant DNA technology by combining nucleotide sequences encoding different peptides or proteins that are otherwise not found together in nature. In the present study, three new fusion proteins were designed, cloned and expressed and assessed f ...
- DOI:
- 10.1016/j.biomaterials.2011.02.040
- PubMed:
- 21458065
- PubMed Central:
- PMC3081935
- https://doi.org/10.1016/j.biomaterials.2011.02.040
- Author:
- Patricia Y.W. Dankers; Jasper M. Boomker; Ali Huizinga-van der Vlag; Eva Wisse; Wilco P.J. Appel; Frank M.M. Smedts; Martin C. Harmsen; Anton W. Bosman; W. Meijer; Marja J.A. van Luyn
- Source:
- Biomaterials 2011 v.32 no.3 pp. 723-733
- ISSN:
- 0142-9612
- Subject:
- artificial membranes; basement membrane; bioactive properties; bioengineering; epithelial cells; extracellular matrix; humans; hydrogen bonding; kidneys; phenotype; polymers
- Abstract:
- ... Maintenance of polarisation of epithelial cells and preservation of their specialized phenotype are great challenges for bioengineering of epithelial tissues. Mimicking the basement membrane and underlying extracellular matrix (ECM) with respect to its hierarchical fiber-like morphology and display of bioactive signals is prerequisite for optimal epithelial cell function in vitro. We report here o ...
- DOI:
- 10.1016/j.biomaterials.2010.09.020
- https://doi.org/10.1016/j.biomaterials.2010.09.020
- Author:
- Maya Gonen-Wadmany; Revital Goldshmid; Dror Seliktar
- Source:
- Biomaterials 2011 v.32 no.26 pp. 6025-6033
- ISSN:
- 0142-9612
- Subject:
- actin; bioactive properties; biocompatible materials; biodegradation; chemical bonding; crosslinking; drugs; fibrin; fibrinogen; fibroblasts; gels; humans; hydrocolloids; mechanical properties; metalloproteinases; polymers; proteolysis; thrombin
- Abstract:
- ... Protein PEGylation has been successfully applied in pharmaceuticals and more recently in biomaterials development for making bioactive and structurally versatile hydrogels. Despite many advantages in this regard, PEGylation of proteins is also known to alter biological activity and modify biophysical characteristics in ways that may be detrimental to cells. The aim of this study was to evaluate th ...
- DOI:
- 10.1016/j.biomaterials.2011.04.055
- https://doi.org/10.1016/j.biomaterials.2011.04.055
- Author:
- Pyung-Hwan Kim; Jaesung Kim; Tae-il Kim; Hye Yeong Nam; James W. Yockman; Minjung Kim; Sung Wan Kim; Chae-Ok Yun
- Source:
- Biomaterials 2011 v.32 no.35 pp. 9328-9342
- ISSN:
- 0142-9612
- Subject:
- adverse effects; blood circulation; blood serum; half life; hepatoma; hepatotoxicity; humans; immune response; interleukins; liver; neoplasm cells; neutralization; polymers; therapeutics; viruses
- Abstract:
- ... Systemic administration of adenovirus (Ad) vectors is complicated by host immune responses and viral accumulation in the liver, resulting in a short circulatory virus half-life, low efficacy, and host side effects. Ad surface modification is thus required to enhance safety and therapeutic efficacy. An arginine-grafted bioreducible polymer (ABP) was chemically conjugated to the Ad surface, generati ...
- DOI:
- 10.1016/j.biomaterials.2011.08.066
- https://doi.org/10.1016/j.biomaterials.2011.08.066
- Author:
- Han Na Yang; Ji Sun Park; Dae Gyun Woo; Su Yeon Jeon; Hyun-Jin Do; Hye-Young Lim; Jae-Hwan Kim; Keun-Hong Park
- Source:
- Biomaterials 2011 v.32 no.25 pp. 5924-5933
- ISSN:
- 0142-9612
- Subject:
- Western blotting; adipogenesis; biodegradability; confocal laser scanning microscopy; cultured cells; fluorescence microscopy; gene overexpression; genes; green fluorescent protein; histology; humans; nanoparticles; nanospheres; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; stem cells; transcription factors; transfection
- Abstract:
- ... In this study, to drive efficient adipogenic differentiation, the adipogenic transcription factors C/EBP-α and C/EBP-β fused to green fluorescent protein (GFP) or red fluorescent protein (RFP) were complexed with poly-ethyleneimine (PEI) coupled with biodegradable PLGA nanospheres and delivered to human mesenchymal stem cell (hMSC). FACS analysis revealed that the transfection efficiency of C/EBP- ...
- DOI:
- 10.1016/j.biomaterials.2011.04.072
- https://doi.org/10.1016/j.biomaterials.2011.04.072
- Author:
- Koki Kamiya; Kanta Tsumoto; Tetsuro Yoshimura; Kazunari Akiyoshi
- Source:
- Biomaterials 2011 v.32 no.36 pp. 9899-9907
- ISSN:
- 0142-9612
- Subject:
- calcium; confocal laser scanning microscopy; dissociation; drug delivery systems; endocytosis; endosomes; humans; pH; proteins; viruses
- Abstract:
- ... N-cadherin (CDH2) proteins were reconstituted with liposomes using a baculovirus expression-liposome fusion method. CDH2 budded viruses were fused with giant liposomes containing dioleoylphophogycerol/dioleoylphosphatidylcholine (DOPG/DOPC) at pH 4.5 and the localization of CDH2 on the liposome membrane was observed by confocal laser scanning microscopy. CDH2 liposomes showed Ca²⁺-dependent associ ...
- DOI:
- 10.1016/j.biomaterials.2011.09.008
- https://doi.org/10.1016/j.biomaterials.2011.09.008
37. Cell transcytosing poly-arginine coated magnetic nanovector for safe and effective siRNA delivery
- Author:
- Omid Veiseh; Forrest M. Kievit; Hyejung Mok; Joseph Ayesh; Cassra Clark; Chen Fang; Matthew Leung; Hamed Arami; James O. Park; Miqin Zhang
- Source:
- Biomaterials 2011 v.32 no.24 pp. 5717-5725
- ISSN:
- 0142-9612
- Subject:
- brain; cell viability; coatings; endocytosis; ethylene glycol; fluorescence; gene silencing; genes; humans; image analysis; iron oxides; nanoparticles; neoplasm cells; neoplasms; physiological transport; polymers; small interfering RNA; therapeutics; toxicity; transmission electron microscopy
- Abstract:
- ... Lack of safe and effective carriers for delivery of RNA therapeutics remains a barrier to its broad clinical application. We report the development of a cell tanscytosing magnetic nanovector engineered as an siRNA carrier. Iron oxide nanoparticles were modified with poly(ethylene glycol) (PEG), small interfering RNA (siRNA), and a cationic polymer layer. Three nanovector formulations with cationic ...
- DOI:
- 10.1016/j.biomaterials.2011.04.039
- PubMed:
- 21570721
- PubMed Central:
- PMC3125526
- https://doi.org/10.1016/j.biomaterials.2011.04.039
- Author:
- Debanjan Sarkar; James A. Ankrum; Grace S.L. Teo; Christopher V. Carman; Jeffrey M. Karp
- Source:
- Biomaterials 2011 v.32 no.11 pp. 3053-3061
- ISSN:
- 0142-9612
- Subject:
- cellular microenvironment; dexamethasone; extracellular matrix; genetic engineering; humans; models; phenotype; receptors; stem cells; therapeutics; tissue repair
- Abstract:
- ... A cell’s fate is tightly controlled by its microenvironment. Key factors contributing to this microenvironment include physical contacts with the extracellular matrix and neighboring cells, in addition to soluble factors produced locally or distally. Alterations to these cues can drive homeostatic processes, such as tissue regeneration/wound healing, or may lead to pathologic tissue dysfunction. I ...
- DOI:
- 10.1016/j.biomaterials.2010.12.036
- PubMed:
- 21262537
- PubMed Central:
- PMC3043463
- https://doi.org/10.1016/j.biomaterials.2010.12.036
- Author:
- Yaseen M. Elkasabi; Joerg Lahann; Paul H. Krebsbach
- Source:
- Biomaterials 2011 v.32 no.7 pp. 1809-1815
- ISSN:
- 0142-9612
- Subject:
- Fourier transform infrared spectroscopy; biocompatible materials; biopharmaceuticals; coatings; composite polymers; enzyme-linked immunosorbent assay; fibroblasts; humans; polymerization; reporter genes; vapors
- Abstract:
- ... Spatiotemporal control of gene delivery, particularly signaling gradients, via biomaterials poses significant challenges because of the lack of efficient delivery systems for therapeutic proteins and genes. This challenge was addressed by using chemical vapor deposition (CVD) polymerization in a counterflow set-up to deposit copolymers bearing two reactive chemical gradients. FTIR spectroscopy ver ...
- DOI:
- 10.1016/j.biomaterials.2010.10.046
- PubMed:
- 21176953
- PubMed Central:
- PMC3021648
- https://doi.org/10.1016/j.biomaterials.2010.10.046
- Author:
- Soumya Ravindran; Jacob L. Roam; Peter K. Nguyen; Thomas M. Hering; Donald L. Elbert; Audrey McAlinden
- Source:
- Biomaterials 2011 v.32 no.33 pp. 8436-8445
- ISSN:
- 0142-9612
- Subject:
- collagen; drugs; ethylene glycol; gene expression; humans; phenotype; protein synthesis; stromal cells; transforming growth factor beta 3
- Abstract:
- ... Biomaterial microparticles are commonly utilized as growth factor delivery vehicles to induce chondrogenic differentiation of mesenchymal stem/stromal cells (MSCs). To address whether the presence of microparticles could themselves affect differentiation of MSCs, a 3D co-aggregate system was developed containing an equal volume of human primary bone marrow-derived MSCs and non-degradable RGD-conju ...
- DOI:
- 10.1016/j.biomaterials.2011.07.056
- PubMed:
- 21820171
- PubMed Central:
- PMC3176960
- https://doi.org/10.1016/j.biomaterials.2011.07.056
- Author:
- Ming Ni; Jeremy C.M. Teo; Mohammed Shahrudin bin Ibrahim; Kangyi Zhang; Farah Tasnim; Pei-Yong Chow; Daniele Zink; Jackie Y. Ying
- Source:
- Biomaterials 2011 v.32 no.6 pp. 1465-1476
- ISSN:
- 0142-9612
- Subject:
- bioreactors; coatings; extracellular matrix; humans; kidney cells; kidneys
- Abstract:
- ... The bioreactor unit of bioartificial kidneys contains porous membranes seeded with renal cells. For clinical applications, it is mandatory that human primary renal proximal tubule cells (HPTCs) form differentiated epithelia on the membranes. Here, we show that HPTCs do not grow and survive on a variety of polymeric membrane materials. This applies also to membranes consisting of polysulfone/polyvi ...
- DOI:
- 10.1016/j.biomaterials.2010.10.061
- https://doi.org/10.1016/j.biomaterials.2010.10.061
- Author:
- Holly D. Barth; Elizabeth A. Zimmermann; Eric Schaible; Simon Y. Tang; Tamara Alliston; Robert O. Ritchie
- Source:
- Biomaterials 2011 v.32 no.34 pp. 8892-8904
- ISSN:
- 0142-9612
- Subject:
- Fourier transform infrared spectroscopy; X-radiation; bone strength; collagen; fluorometry; humans; hydroxyapatite; irradiation; mechanical properties; micro-computed tomography; scanning electron microscopy; therapeutics
- Abstract:
- ... Bone comprises a complex structure of primarily collagen, hydroxyapatite and water, where each hierarchical structural level contributes to its strength, ductility and toughness. These properties, however, are degraded by irradiation, arising from medical therapy or bone-allograft sterilization. We provide here a mechanistic framework for how irradiation affects the nature and properties of human ...
- DOI:
- 10.1016/j.biomaterials.2011.08.013
- PubMed:
- 21885114
- PubMed Central:
- PMC4405888
- https://doi.org/10.1016/j.biomaterials.2011.08.013
- Author:
- Ming-Jun Tsai; Yi-Hung Tsai; Yu-Min Kuo
- Source:
- Biomaterials 2011 v.32 no.27 pp. 6381-6388
- ISSN:
- 0142-9612
- Subject:
- animal models; biocompatibility; blood flow; carotid arteries; cerebellum; chitin; correlation; cortex; embolism; ganglia; hippocampus; humans; infarction; intravenous injection; mixing; rats; stroke; thalamus
- Abstract:
- ... Embolism is responsible for half of cerebral infarctions, yet few animal models were developed due to the unpredictability of the embolus-induced infarcts. We manufactured artificial embolic particles by blending chitin and poly(d,l-Lactide-co-glycolide) (chitin/PLGA) for their good biocompatibility and rapid hydration expansion property. We subdivided the chitin/PLGA microparticles into 10 size g ...
- DOI:
- 10.1016/j.biomaterials.2011.05.051
- https://doi.org/10.1016/j.biomaterials.2011.05.051
- Author:
- Ji S. Park; Han N. Yang; Dae G. Woo; Su Y. Jeon; Keun-H. Park
- Source:
- Biomaterials 2011 v.32 no.6 pp. 1495-1507
- ISSN:
- 0142-9612
- Subject:
- Western blotting; animal models; cartilage; cell differentiation; chondrogenesis; denaturation; extracellular matrix; fibrin; genes; humans; hydrocolloids; immunohistochemistry; in vitro culture; in vivo studies; mice; protein synthesis; quantitative polymerase chain reaction; rabbits; reverse transcriptase polymerase chain reaction; stem cells; transforming growth factor beta 3
- Abstract:
- ... In this study, hMSCs encapsulated in a fibrin hydrogel containing heparinized NPs loaded with TGF-β3 (100 ng/ml), or TGF-β3 (100 ng/ml) alone, were subjected to growth factor release and denaturation tests at one, two and four weeks in in vitro culture systems. Additionally, stem cell differentiation was assessed via RT-PCR, real-time quantitative PCR (qPCR), histology, and immunohistochemical ass ...
- DOI:
- 10.1016/j.biomaterials.2010.11.003
- https://doi.org/10.1016/j.biomaterials.2010.11.003
- Author:
- Ji Sun Park; Han Na Yang; Dae Gyun Woo; Su Yeon Jeon; Hyun-Jin Do; Hye-Young Lim; Jae-Hwan Kim; Keun-Hong Park
- Source:
- Biomaterials 2011 v.32 no.14 pp. 3679-3688
- ISSN:
- 0142-9612
- Subject:
- cell differentiation; chondrogenesis; ethyleneimine; fluorescence; green fluorescent protein; humans; in vitro culture; nanoparticles; polymers; reporter genes; stem cells; therapeutics; transfection
- Abstract:
- ... Target gene transfection for desired cell differentiation has recently become a major issue in stem cell therapy. For the safe and stable delivery of genes into human mesenchymal stem cells (hMSCs), we employed a non-viral gene carrier system such as polycataionic polymer, poly(ethyleneimine) (PEI), polyplexed with a combination of SOX5, 6, and 9 fused to green fluorescence protein (GFP), yellow f ...
- DOI:
- 10.1016/j.biomaterials.2011.01.063
- https://doi.org/10.1016/j.biomaterials.2011.01.063
- Author:
- Mingliang You; Gongfeng Peng; Jian Li; Ping Ma; Zhihui Wang; Weiliang Shu; Siwu Peng; Guo-Qiang Chen
- Source:
- Biomaterials 2011 v.32 no.9 pp. 2305-2313
- ISSN:
- 0142-9612
- Subject:
- binding proteins; blood serum; bone marrow; cartilage; cell adhesion; coatings; collagen; extracellular matrix; genes; glycosaminoglycans; humans; hydrophobicity; image analysis; polyhydroxyalkanoates; scanning electron microscopy; stem cells; tissue engineering
- Abstract:
- ... Hydrophobic polyhydroxyalkanoate (PHA) scaffolds made of a copolyester of 3-hydroxybutyrate-co-hydroxyhexanoate (PHBHHx) were coated with a fusion protein PHA granule binding protein PhaP fused with RGD peptide (PhaP-RGD). Human bone marrow mesenchymal stem cells (hBMSCs) were inoculated on/in the scaffolds for formation of articular cartilages derived from chondrogenic differentiation of hBMSCs f ...
- DOI:
- 10.1016/j.biomaterials.2010.12.009
- https://doi.org/10.1016/j.biomaterials.2010.12.009
47. Computed tomography imaging of cancer cells using acetylated dendrimer-entrapped gold nanoparticles
- Author:
- Han Wang; Linfeng Zheng; Chen Peng; Rui Guo; Mingwu Shen; Xiangyang Shi; Guixiang Zhang
- Source:
- Biomaterials 2011 v.32 no.11 pp. 2979-2988
- ISSN:
- 0142-9612
- Subject:
- X-radiation; acetylation; biocompatibility; cell cycle; cell viability; computed tomography; flow cytometry; gold; humans; image analysis; intraperitoneal injection; lysosomes; models; nanogold; neoplasm cells; neutralization; transmission electron microscopy
- Abstract:
- ... We report a new use of acetylated dendrimer-entrapped gold nanoparticles (Au DENPs) for in vitro and in vivo computed tomography (CT) imaging of cancer cells. In this study, Au DENPs prepared using amine-terminated generation 5 poly(amidoamine) dendrimers were subjected to an acetylation reaction to neutralize the positive surface potential. The acetylated Au DENPs were used for both in vitro and ...
- DOI:
- 10.1016/j.biomaterials.2011.01.001
- https://doi.org/10.1016/j.biomaterials.2011.01.001
- Author:
- Haibao Zhu; Binrui Cao; Zipeng Zhen; Ayyagari A. Laxmi; Dong Li; Shaorong Liu; Chuanbin Mao
- Source:
- Biomaterials 2011 v.32 no.21 pp. 4744-4752
- ISSN:
- 0142-9612
- Subject:
- bacteriophages; chemistry; humans; nanofibers; osteoblasts; peptides; proteins; stem cells; tissue engineering; topography
- Abstract:
- ... The search for a cell-supporting scaffold with controlled topography and surface chemistry is a constant topic within tissue engineering. Here we have employed M13 phages, which are genetically modifiable biological nanofibers (∼880 nm long and ∼6.6 nm wide) non-toxic to human beings, to form films for supporting the growth of mesencymal stem cells (MSCs). Films were built from nearly parallel pha ...
- DOI:
- 10.1016/j.biomaterials.2011.03.030
- PubMed:
- 21507480
- PubMed Central:
- PMC3099180
- https://doi.org/10.1016/j.biomaterials.2011.03.030
- Author:
- Shan Wu; Alan Wells; Linda G. Griffith; Douglas A. Lauffenburger
- Source:
- Biomaterials 2011 v.32 no.30 pp. 7524-7531
- ISSN:
- 0142-9612
- Subject:
- biomimetics; cell movement; collagen; decision support systems; drugs; epidermal growth factor; ethylene oxide; extracellular matrix; fibronectins; humans; mitogen-activated protein kinase; models; polymers; stromal cells; tissue repair
- Abstract:
- ... Biomimetic scaffolds have been proposed as a means to facilitate tissue regeneration by multi-potent stromal cells (MSCs). Effective scaffold colonization requires a control of multiple MSC responses including survival, proliferation, differentiation, and migration. As MSC migration is relatively unstudied in this context, we present here a multi-level approach to its understanding and control, in ...
- DOI:
- 10.1016/j.biomaterials.2011.06.050
- PubMed:
- 21782235
- PubMed Central:
- PMC3156355
- https://doi.org/10.1016/j.biomaterials.2011.06.050
- Author:
- Amit K. Jha; Xian Xu; Randall L. Duncan; Xinqiao Jia
- Source:
- Biomaterials 2011 v.32 no.10 pp. 2466-2478
- ISSN:
- 0142-9612
- Subject:
- adhesion; adipogenesis; bone formation; collagen; crosslinking; emulsions; gelatin; humans; hyaluronic acid; hydrocolloids; polymerization; pseudopodia; sowing; stem cells
- Abstract:
- ... We have created hyaluronic acid (HA)-based, cell-adhesive hydrogels that direct the initial attachment and the subsequent differentiation of human mesenchymal stem cells (MSCs) into pre-osteoblasts without osteogenic supplements. HA-based hydrogel particles (HGPs) with an average diameter of 5–6 μm containing an estimated 2.2 wt% gelatin (gHGPs) were synthesized by covalent immobilization of gelat ...
- DOI:
- 10.1016/j.biomaterials.2010.12.024
- PubMed:
- 21216457
- PubMed Central:
- PMC3032013
- https://doi.org/10.1016/j.biomaterials.2010.12.024