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- Prazeres, Duarte M. F., et al. Show all 5 Authors
- Bioprocess and biosystems engineering 2009 v.32 no.5 pp. 615-623
- Escherichia coli; RNA; alcohols; anion exchange; bioprocessing; chromatography; filtration; plasmids; salts
- ... A new bioprocess using mainly membrane operations to obtain purified plasmid DNA from Escherechia coli ferments was developed. The intermediate recovery and purification of the plasmid DNA in cell lysate was conducted using hollow-fiber tangential filtration and tandem anion-exchange membrane chromatography. The purity of the solutions of plasmid DNA obtained during each process stage was investig ...