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You searched for: Journal name Bioscience, biotechnology, and biochemistry Remove constraint Journal name: Bioscience, biotechnology, and biochemistry Publication year rev 8003-1997 Remove constraint Publication year rev: 8003-1997 Source 1997 v.61 no.6 Remove constraint Source: 1997 v.61 no.6
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1. Analysis of Water Sorption Isotherms of Superabsorbent Polymers by Solution Thermodynamics

Author:
Hitoshi Kumagai; Akinori Mizuno; Hitomi Kumagai; Toshimasa Yano
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 936-941
ISSN:
1347-6947
Subject:
Gibbs free energy; biochemistry; biotechnology; crosslinking; sorption; transportation; water activity; water analysis; water content
Abstract:
... Water sorption isotherms of superabsorbent polymers were measured, and their affinity for water was evaluated by solution thermodynamics. The results provide basic data for the functional packaging of food to control the water content of food during its transportation or storage. Water activity above 0.9 was measured by adding a specific amount of water to the samples, while that below 0.9 was mea ...
DOI:
10.1271/bbb.61.936

2. A Bacteriocin of Strain Pediococcus sp. ISK-1 Isolated from Nukadoko, Bed of Fermented Rice Bran

Author:
Hirokazu Kimura; Rie Nagano; Hiromi Matsusaki; Kenji Sonomoto; Ayaaki Ishizaki
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 1049-1051
ISSN:
1347-6947
Subject:
Pediococcus; antimicrobial properties; autoclaving; biotechnology; fermentation; lactic acid; pH; pediocins; rice bran
Abstract:
... Pediococcus sp. ISK-1 isolated in our laboratory from well-aged Nukadoko, produces a bacteriocin which has a unique antimicrobial spectrum among pediocins. The bacteriocin was stable at acidic pH, and more than 60% of the antimicrobial activity still remained even after being autoclaved at 121°C for 20min in the pH range of 3 to 8. This is the first report dealing with a bacteriocin produced by la ...
DOI:
10.1271/bbb.61.1049

3. Changes in Messenger RNA of Pancreatic Enzymes and Intestinal Cholecystokinin after a 7-Day Bile-pancreatic Juice Diversion from the Proximal Small Intestine in Rats

Author:
Hiroshi Hara; Yasuo Ochi; Takanori Kasai
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 1002-1006
ISSN:
1347-6947
Subject:
amylases; biotechnology; cholecystokinin; chymotrypsinogen; duodenum; gene expression; ileum; jejunum; juices; messenger RNA; mucosa; pancreas; secretion; trypsinogen
Abstract:
... We have previously demonstrated the bile-pancreatic juice (BPJ)-independent stimulation of pancreatic enzyme secretion in chronic BPJ-diverted rats. Pancreatic and intestinal adaptation to 7-day BPJ diversion was next examined. Pancreatic enzyme mRNA and cholecystokinin mRNA in the jejunal mucosa were measured in rats with BPJ diverted into the ileum (PBD rats) in comparison with the figures for r ...
DOI:
10.1271/bbb.61.1002

4. Competitive ELISA of Bovine Lactoferrin with Bispecific Monoclonal Antibodies

Author:
Hiroshi Shinmoto; Masuko Kobori; Tojiro Tsushida; Kazuki Shinohara
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 1044-1046
ISSN:
1347-6947
Subject:
antigens; biotechnology; chromatography; enzyme-linked immunosorbent assay; lactoferrin; mice; peroxidase
Abstract:
... A mouse hybrid-hybridoma, HH1–4–3, secreting IgG₁ class bispecific antibodies to bovine lactoferrin (bLF) and horseradish peroxidase (HRPO), was established previously. The competitive enzyme-linked immunosorbent assay (ELISA) of bLF using the HH1–4–3 culture supernatant was not sensitive enough to measure bLF concentration in biological fluids. To improve the sensitivity of the competitive ELISA, ...
DOI:
10.1271/bbb.61.1044

5. Determination of Endogenous Peptides with in Vitro ACE Inhibitory Activity in Normotensive Human Plasma by the Fluorometric HPLC Method

Author:
Toshiro Matsui; Hiroshi Matsufuji; Terukazu Kawasaki; Yutaka Osajima
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 1052-1054
ISSN:
1347-6947
Subject:
biotechnology; enzyme inhibitors; fluorometry; humans; males; metabolites; peptides; peptidyl-dipeptidase A
Abstract:
... An in vitro degradation test of angiotensin (ANG) II or III in normotensive supine human plasma from 9 healthy male subjects confirmed the production of smaller ANG metabolites with angiotensin I-converting enzyme inhibitory activity. These metabolites were identified as ANG (3–8), ANG (4–8), ANG (5–8), and ANG (3–4), whose respective peptide concentrations were determined by our proposed naphthal ...
DOI:
10.1271/bbb.61.1052

6. Effects of DNA Topology on Transformation Efficiency of Bacillus subtilis ISW1214 by Electroporation

Author:
Morimasa Ohse; Koji Kawade; Hideo Kusaoke
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 1019-1021
ISSN:
1347-6947
Subject:
Bacillus subtilis; Gram-positive bacteria; biotechnology; calves; capacitance; electric field; electroporation; molecular weight; plasmids; restriction endonucleases; topology
Abstract:
... We report an investigation of electrotransformation by three different topological isomers, circular supercoiled (sc DNA), circular relaxed (cr DNA), and linearized (In DNA) forms of the plasmids pUB110 (4.5 kbp) and pBDR331T (12.6 kbp), of a Gram-positive bacterium, Bacillus subtilis ISW1214. Treatment of the sc DNA with calf thymus topoisomerase I removed the superhelicity and the DNA assumed th ...
DOI:
10.1271/bbb.61.1019

7. Enhanced Peroxidation of Proteins of the Erythrocyte Membrane and of Muscle Tissue by Dietary Oxidized Oil

Author:
Israela Hayam; Uri Cogan; Shoshana Mokady
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 1011-1012
ISSN:
1347-6947
Subject:
biotechnology; erythrocyte membrane; ingestion; males; muscle tissues; oxidation; oxidative stress; peroxidation; soybean oil; spectrin; thiols; weanlings
Abstract:
... Male weanling rats were fed on diets containing 10% of either oxidized or fresh (control) soybean oil for periods of 4 and 7 weeks. The ingestion of oxidized oil was found to reduce the content of free thiols in the erythrocyte membrane and to increase the amount of membrane-extractable spectrin. The level of reactive carbonyl groups was higher in the proteins of the ghosts and of the muscle tissu ...
DOI:
10.1271/bbb.61.1011

8. Heterologous Protein Production in Acremonium chrysogenum: Expression of Bacterial Cephalosporin C Acylase and Human Thrombomodulin Genes

Author:
Goichi Honda; Akio Matsuda; Michitaka Zushi; Shuji Yamamoto; Ken-ichi Komatsu
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 948-955
ISSN:
1347-6947
Subject:
Acremonium chrysogenum; Pseudomonas; biotechnology; cephalosporins; culture media; epidermal growth factor; genes; heterologous gene expression; humans; immunochemistry; mutants; phosphoglycerate kinase; plasmids
Abstract:
... We have developed an efficient expression system for foreign genes in Acremonium chrysogenum. After inserting the foreign gene between the phosphoglycerate kinase (PGK) promoter and a terminator derived from A. chrysogenum, multiple copies of this expression unit are tandemly ligated into cosmids and the resultant cosmids are introduced into A. chrysogenum. We expressed Pseudomonas cephalosporin C ...
DOI:
10.1271/bbb.61.948

9. Inactivation of Bacillus Spores by the Supercritical Carbon Dioxide Micro-bubble Method

Author:
Hiroya Ishikawa; Mitsuya Shimoda; Kei Tamaya; Akiyoshi Yonekura; Tamotsu Kawano; Yutaka Osajima
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 1022-1023
ISSN:
1347-6947
Subject:
Bacillus cereus; biochemistry; biotechnology; carbon dioxide; heat treatment; microbubbles
Abstract:
... Bacillus spores were effectively inactivated by the supercritical (SC) CO₂ micro-bubble method. The micro-bubble SC CO₂ treatment of B. cereus, B. subtilis, B. megaterium, B. polymyxa, and B. coagulans at 40°C and 30 MPa for 30min produced greater reduction (about 3 log cycles of reduction) than a similar treatment without a filter. The SC CO₂ treatment of B. polymyxa, B. cereus, and B. subtilis s ...
DOI:
10.1271/bbb.61.1022

10. Inhibitory Effect of Alginic Acids on Hyaluronidase and on Histamine Release from Mast Cells

Author:
Masahiro Asada; Makiko Sugie; Mami Inoue; Kazuya Nakagomi; Seiji Hongo; Katsumi Murata; Shinji Irie; Toshio Takeuchi; Noboru Tomizuka; Syuichi Oka
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 1030-1032
ISSN:
1347-6947
Subject:
alginates; biotechnology; esterification; histamine; hyaluronoglucosaminidase; mast cells; molecular weight
Abstract:
... The effects of various types of alginic acid consisting of l-guluronic acids (G) and d-mannuronic acids (M) on hyaluronidase and mast cell degranulation were examined. Alginic acid with an M/G ratio of 1.0 exhibited the strongest inhibition of both activities, the higher molecular weight alginic acids of 150 to 370 kDa being preferable in both cases. Esterification of the carboxyl residue enhanced ...
DOI:
10.1271/bbb.61.1030

11. Kinetics for the Autoxidation of Triacylglycerols Containing Eicosapentaenoic Acid

Author:
Yasushi Endo; Sanae Hoshizaki; Kenshiro Fujimoto
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 1036-1037
ISSN:
1347-6947
Subject:
autoxidation; biotechnology; eicosapentaenoic acid; palmitic acid; triacylglycerols
Abstract:
... The oxidative rate of three kinds of synthetic triacylglycerols (TAGs) consisting of eicosapentaenoic acid (EPA) and palmitic acid was measured by the induction period method to understand the mechanism for the autoxidation of TAGs containing highly unsaturated fatty acids (HUFAs) such as marine oils. The propagation rate, oxidizability, and kinetic chain length increased with the number of moles ...
DOI:
10.1271/bbb.61.1036

12. Long-term Culture of Primary Rat Hepatocytes on Heparin- or Lambda Carrageenan-containing Collagen Gels

Author:
Kong Hua Lin; Sumio Maeda; Hidetoshi Inagaki; Takao Saito
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 971-974
ISSN:
1347-6947
Subject:
albumins; biotechnology; cell adhesion; cell differentiation; collagen; heparin; hepatocytes; rats
Abstract:
... The interactions of glycosaminoglycans (GAGs) with collagen are thought to be important in cell adhesion and cell differentiation. To investigate whether the interactions of GAG or sulfated polysaccharide with collagen can maintain the functions of cultured primary rat hepatocytes, GAG- or sulfated polysaccharide-containing collagen gels were reconstituted in vitro and used for culture of hepatocy ...
DOI:
10.1271/bbb.61.971

13. Metabolism of DFA III by Arthrobacter sp. H65-7: Purification and Properties of a DFA III Hydrolysis Enzyme (DFA IIIase)

Author:
Hiroaki Sakurai; Atsushi Yokota; Yoko Sumita; Yumiko Mori; Hirokazu Matsui; Fusao Tomita
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 989-993
ISSN:
1347-6947
Subject:
Arthrobacter; ammonium sulfate; biotechnology; enzymes; fractionation; fructose; gel chromatography; heat treatment; hydrolysis; metabolism; molecular weight; pH; polyacrylamide gel electrophoresis
Abstract:
... Assimilation of DFA III by Arthrobacter sp. H65-7 was found to consist of two sequential enzyme steps, hydrolysis of DFA III to inulobiose (1-O-β-d-fructofuranosyl-d-fructopyranose) and inulobiose to fructose, that is, α-(2 → 3′) and β-(2′ → 1) fructosidic linkages were split separately. The enzyme catalyzing the first step, named DFA III hydrolysis enzyme (DFA IIIase), has been purified from the ...
DOI:
10.1271/bbb.61.989

14. Purification and Characterization of Soybean Allergen Gly m Bd 28K

Author:
Hideaki Tsuji; Noriko Bando; Miki Hiemori; Rintaro Yamanishi; Masumi Kimoto; Kiyoshi Nishikawa; Tadashi Ogawa
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 942-947
ISSN:
1347-6947
Subject:
allergenicity; allergens; amino acid sequences; atopic dermatitis; biotechnology; glycoproteins; immunoaffinity chromatography; immunoblotting; ligands; moieties; molecular weight; monoclonal antibodies; purification methods; soybeans; sugars
Abstract:
... At least 15 allergenic proteins have been found in soybean using the sera of soybean-sensitive patients with atopic dermatitis [T. Ogawa et al., J. Nutr. Sci. Vitaminol., 35, 555–565 (1991)]. In the present study, a monoclonal antibody (mAb) against Gly m Bd 28K, one of the major allergens of soybean, was prepared, and Gly m Bd 28K was purified from defatted soybean flakes by five purification ste ...
DOI:
10.1271/bbb.61.942

15. Purification and Characterization of a Glucose-tolerant β-Glucosidase from Aspergillus niger CCRC 31494

Author:
Tsong-Rong Yan; Chun-Lieh Lin
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 965-970
ISSN:
1347-6947
Subject:
Aspergillus niger; biotechnology; cellobiose; fractionation; fructose; galactose; glucose; mannose; methanol; pH; serine; thiols; xylose
Abstract:
... An extracellular glucose-tolerant β-glucosidase was purified to homogeneity by alcohol fractionation and preparative isoelectric focusing from Aspergillus niger CCRC 31494. The enzyme was a dimeric protein with a subunit of 49,000, and had its optimum activity at pH 5.0 and 55°C. The enzyme was completely inhibited by 5 mm Ag⁺. Thiol groups and serine residues were not essential for its activity. ...
DOI:
10.1271/bbb.61.965

16. Purification and Properties of a New Enzyme, D-Carnitine Dehydrogenase, from Agrobacterium sp. 525a

Author:
Siswa Setyahadi; Tomoko Ueyama; Takeshi Arimoto; Nobuhiro Mori; Yutaka Kitamoto
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 1055-1058
ISSN:
1347-6947
Subject:
Agrobacterium; affinity chromatography; ammonium sulfate; biotechnology; filtration; fractionation; molecular weight; oxidation; oxidoreductases; pH
Abstract:
... A new enzyme, d-carnitine dehydrogenase from Agrobacterium sp. 525a, was purified by DEAE-Toyopearl, ammonium sulfate fractionation, Sephadex G-75, affinity chromatography, and Mono Q and TSK-gel filtration column chromatography. The enzyme had the molecular mass of 89 kDa and consisted of three identical subunits. The optimum pH for the oxidation reaction was 9.3. The Michaelis constants for d-ca ...
DOI:
10.1271/bbb.61.1055

17. Randomly Amplified Polymorphic DNA (RAPD) for Rapid Identification of the Spoilage Bacterium Alicyclobacillus acidoterrestris

Author:
Koji Yamazaki; Tsutomu Okubo; Norio Inoue; Haruo Shinano
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 1016-1018
ISSN:
1347-6947
Subject:
Alicyclobacillus acidoterrestris; DNA; biotechnology; spoilage bacteria
Abstract:
... RAPD (randomly amplified polymorphic DNA) analysis was developed for rapid identification of the spoilage bacterium, Alicyclobacillus acidoterrestris. Three primers, Ba-10, F-61, and F-64, allowed adequate discrimination between strains of A. acidoterrestris and related bacteria in RAPD analyses. As compared with the conventional method and a RAPD assay against the isolates from various environmen ...
DOI:
10.1271/bbb.61.1016

18. Separation of Caffeine from Supercritical Carbon Dioxide with a Zeolite Membrane

Author:
Yoshihiro Tokunaga; Tomoyuki Fujii; Kozo Nakamura
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 1024-1026
ISSN:
1347-6947
Subject:
biochemistry; biotechnology; caffeine; carbon dioxide; pervaporation; porosity; zeolites
Abstract:
... Caffeine was separated from supercritical carbon dioxide with a zeolite membrane that had been tested for pervaporation. The pore size of the NaY zeolite membrane was evaluated to be of subnanometer scale from the result of a Dubinin-Astakhov analysis. The rejection of caffeine was 0.98 by the zeolite membrane which would make it applicable for SCCO₂ membrane separation. ...
DOI:
10.1271/bbb.61.1024

19. Structural Analysis of Disaccharides Synthesized by β-d-Glucosidase of Bifidobacterium hreve clb and Their Assimilation by Bifidobacteria

Author:
Naoki Nunoura; Tomoyuki Fujita; Kohji Ohdan; Mitsunori Kirihata; Kenji Yamamoto; Hidehiko Kumagai
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 1033-1035
ISSN:
1347-6947
Subject:
Bifidobacterium breve; activated carbon; biotechnology; gentiobiose; glucose; intestines
Abstract:
... Circulation of a solution of 1 m d-fucose and 1 m d-glucose through a reaction system consisting of serial columns of immobilized recombinant β-d-glucosidase of Bifidobacterium breve clb and activated charcoal gave two oligosaccharides. Structural analysis identified these oligosaccharides as d-fucosylglucose (6-O-β-d-Fucopyranosyl-d-glucose) and gentiobiose (6-O-β-d-Glucopyranosyl-d-glucose). The ...
DOI:
10.1271/bbb.61.1033

20. The Sugar Specificity of a Na⁺/Glucose Cotransporter from Rat Jejunum

Author:
Hitoshi Aoshima; Terufumi Yokoyama; Junko Tanizaki; Hanae Izu; Mamoru Yamada
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.6 pp. 979-983
ISSN:
1347-6947
Subject:
Chlorella; Xenopus; biotechnology; cDNA libraries; dose response; galactose; glucose; isomerization; jejunum; oocytes; rats; reaction kinetics; symporters; transportation
Abstract:
... A cDNA for a Na ⁺/glucose cotransporter was cloned from a rat jejunum cDNA library. This transporter was expressed in Xenopus oocytes by injection of cRNA synthesized from the cDNA, and the transporter ability was electrophysiologically examined. The cotransporter had a very narrow sugar specificity. Only d-glucose, d-galactose, and some of their derivatives elicited significant electrical respons ...
DOI:
10.1271/bbb.61.979