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You searched for: Journal name Biosensors & bioelectronics Remove constraint Journal name: Biosensors & bioelectronics Publication year rev 7986-2014 Remove constraint Publication year rev: 7986-2014 Source 2014 v.56 Remove constraint Source: 2014 v.56
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1. Absorbance enhancement in microplate wells for improved-sensitivity biosensors

Author:
Guillaume Suárez; Christian Santschi; Gregory Plateel; Olivier J.F. Martin; Michael Riediker
Source:
Biosensors & bioelectronics 2014 v.56 pp. 198-203
ISSN:
0956-5663
Subject:
absorbance; bioassays; biosensors; colorimetry; cytochrome c; glass fibers; peroxidase; refractive index
Abstract:
... A generic optical biosensing strategy was developed that relies on the absorbance enhancement phenomenon occurring in a multiple scattering matrix. Experimentally, inserts made of glass fiber membrane were placed into microplate wells in order to significantly lengthen the trajectory of the incident light through the sample and therefore increase the corresponding absorbance. Enhancement factor wa ...
DOI:
10.1016/j.bios.2013.12.063

2. Development of an ultrasensitive aptasensor for the detection of aflatoxin B1

Author:
Xiaodong Guo; Fang Wen; Nan Zheng; Qiujiang Luo; Haiwei Wang; Hui Wang; Songli Li; Jiaqi Wang
Source:
Biosensors & bioelectronics 2014 v.56 pp. 340-344
ISSN:
0956-5663
Subject:
aflatoxin B1; biosensors; complementary DNA; detection limit; feed contamination; feeds; financial economics; food safety; foods; hay; oligonucleotides; quantitative analysis; quantitative polymerase chain reaction; rapid methods; rice; toxicity
Abstract:
... Contamination of feed and food by aflatoxin B1 (AFB1), one of the most toxic of the mycotoxins, is a global concern. To prevent food safety scares, and avoid subsequent economic losses due to the recall of contaminated items, methods for the rapid, sensitive and specific detection of AFB1 at trace levels are much in demand. In this work, a simple, ultrasensitive, and reliable aptasensor is describ ...
DOI:
10.1016/j.bios.2014.01.045

3. Electrochemical aptasensor of cellular prion protein based on modified polypyrrole with redox dendrimers

Author:
A. Miodek; G. Castillo; T. Hianik; H. Korri-Youssoufi
Source:
Biosensors & bioelectronics 2014 v.56 pp. 104-111
ISSN:
0956-5663
Subject:
biosensors; biotin; blood plasma; detection limit; diffusivity; electrochemistry; gold; humans; ions; oligonucleotides; prions; streptavidin
Abstract:
... This work consists of the development of an electrochemical aptasensor based on polyprrole modified with redox dendrimers, able to detect human cellular prions PrPC with high sensitivity. The gold surface was modified by conductive polypyrrole film coupled to polyamidoamine dendrimers of fourth generation (PAMAM G4) and ferrocenyl group as redox marker. The aptamers were immobilized on the surface ...
DOI:
10.1016/j.bios.2013.12.051

4. Enhancement of bioelectricity generation by cofactor manipulation in microbial fuel cell

Author:
Xiao-Yu Yong; Jiao Feng; Yi-Lu Chen; Dong-Yan Shi; Yu-Shang Xu; Jun Zhou; Shu-Ya Wang; Lin Xu; Yang-Chun Yong; Yong-Ming Sun; Chen-Lu Shi; Ping-Kai OuYang; Tao Zheng
Source:
Biosensors & bioelectronics 2014 v.56 pp. 19-25
ISSN:
0956-5663
Subject:
NAD (coenzyme); Pseudomonas; bioelectricity; bioenergy; biosensors; electrochemistry; gene overexpression; genes; high performance liquid chromatography; metabolic engineering; microbial fuel cells; organic wastes; pyocyanin
Abstract:
... Microbial fuel cells (MFCs) are promising for harnessing bioenergy from various organic wastes. However, low electricity power output (EPT) is one of the major bottlenecks in the practical application of MFCs. In this study, EPT improvement by cofactor manipulation was explored in the Pseudomonas aeruginosa-inoculated MFCs. By overexpression of nadE (NAD synthetase gene), the availability of the i ...
DOI:
10.1016/j.bios.2013.12.058

5. Enzyme-free and label-free ultrasensitive electrochemical detection of DNA and adenosine triphosphate by dendritic DNA concatamer-based signal amplification

Author:
Shufeng Liu; Ying Lin; Tao Liu; Chuanbin Cheng; Wenji Wei; Li Wang; Feng Li
Source:
Biosensors & bioelectronics 2014 v.56 pp. 12-18
ISSN:
0956-5663
Subject:
DNA; DNA probes; adenosine triphosphate; biosensors; cost effectiveness; detection limit; electrochemistry; medicine
Abstract:
... Hybridization chain reaction (HCR) strategy has been well developed for the fabrication of various biosensing platforms for signal amplification. Herein, a novel enzyme-free and label-free ultrasensitive electrochemical DNA biosensing platform for the detection of target DNA and adenosine triphosphate (ATP) was firstly proposed, in which three auxiliary DNA probes were ingeniously designed to cons ...
DOI:
10.1016/j.bios.2013.12.066

6. G-quadruplex based two-stage isothermal exponential amplification reaction for label-free DNA colorimetric detection

Author:
Ji Nie; De-Wen Zhang; Cai Tie; Ying-Lin Zhou; Xin-Xiang Zhang
Source:
Biosensors & bioelectronics 2014 v.56 pp. 237-242
ISSN:
0956-5663
Subject:
DNA; biosensors; colorimetry; detection limit; genetic disorders; mutants; nucleotide sequences
Abstract:
... A novel G-quadruplex based two-stage isothermal exponential amplification reaction (GQ-EXPAR) was developed for label-free DNA colorimetric detection in this work. The exponential amplified trigger DNA in the first stage can convert into G-quadruplex sequence EAD2 by a linear amplification circuit in the second stage. Created EAD2 can form G-quadruplex/hemin DNAzyme to act as a direct signal reado ...
DOI:
10.1016/j.bios.2014.01.032

7. Highly sensitive glucose biosensor based on Au–Ni coaxial nanorod array having high aspect ratio

Author:
Che-Wei Hsu; Gou-Jen Wang
Source:
Biosensors & bioelectronics 2014 v.56 pp. 204-209
ISSN:
0956-5663
Subject:
ascorbic acid; biosensors; detection limit; electrodes; glucose; gold; nanorods; nickel; uric acid
Abstract:
... An effective glucose biosensor requires a sufficient amount of GOx immobilizing on the electrode surface. An electrode of a 3D nanorod array, having a larger surface-to-volume ratio than a 2D nanostructure, can accommodate more GOx molecules to immobilize onto the surface of the nanorods. In this study, a highly sensitive Au–Ni coaxial nanorod array electrode fabricated through the integration of ...
DOI:
10.1016/j.bios.2014.01.023

8. Investigations of an electrochemical platform based on the layered MoS2–graphene and horseradish peroxidase nanocomposite for direct electrochemistry and electrocatalysis

Author:
Haiyan Song; Yongnian Ni; Serge Kokot
Source:
Biosensors & bioelectronics 2014 v.56 pp. 137-143
ISSN:
0956-5663
Subject:
Fourier transform infrared spectroscopy; X-ray diffraction; biocompatibility; biosensors; detection limit; dielectric spectroscopy; electrochemistry; electron transfer; electrostatic interactions; environmental assessment; hydrogen peroxide; industrial applications; nanocomposites; nanomaterials; peroxidase; transmission electron microscopy
Abstract:
... The self-assembly of layered molybdenum disulfide–graphene (MoS2–Gr) and horseradish peroxidase (HRP) by electrostatic attraction into a novel hybrid nanomaterial (HRP–MoS2–Gr) is reported. The properties of the MoS2–Gr were characterized by X-ray diffraction (XRD), high-resolution transmission electron microscopy (TEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). UV– ...
DOI:
10.1016/j.bios.2014.01.014

9. Lateral flow biosensor for DNA extraction-free detection of salmonella based on aptamer mediated strand displacement amplification

Author:
Zhiyuan Fang; Wei Wu; Xuewen Lu; Lingwen Zeng
Source:
Biosensors & bioelectronics 2014 v.56 pp. 192-197
ISSN:
0956-5663
Subject:
DNA; Salmonella Enteritidis; biosensors; diagnostic techniques; food pathogens; oligonucleotides; rapid methods
Abstract:
... Convenient and sensitive point-of-care rapid diagnostic tests for food-borne pathogens have been a long-felt need of clinicians. Traditional approaches such as culture-based methods have good sensitivity and specificity, but they tend to be tedious and time-consuming. Herein we present a simple and sensitive aptamer based biosensor for rapid detection of Salmonella enteritidis (S. enteritidis). On ...
DOI:
10.1016/j.bios.2014.01.015

10. Liquid crystal based sensors monitoring lipase activity: A new rapid and sensitive method for cytotoxicity assays

Author:
Zakir Hussain; Christian Zafiu; Seta Küpcü; Lucineia Pivetta; Nadine Hollfelder; Akira Masutani; Pinar Kilickiran; Eva-Kathrin Sinner
Source:
Biosensors & bioelectronics 2014 v.56 pp. 210-216
ISSN:
0956-5663
Subject:
acetaminophen; apoptosis; biosensors; cell viability; cytotoxicity; fluorescence; freeze-thaw cycles; liquid crystals; monitoring; necrosis; phospholipases; phospholipids; sodium azide; temperature
Abstract:
... In this work we present liquid crystal (LC) based sensor devices to monitor cell viability. The sensing layer is composed by the LC and a planar monolayer of phospholipids. In the presence of minute traces of phospholipases, which hydrolyze enzymatically phospholipids, the LC–lipid interface is disintegrated. This event causes a change in orientation of the LC, which was followed in a polarized mi ...
DOI:
10.1016/j.bios.2013.12.055

11. Nonenzymatic immunosensor for detection of carbohydrate antigen 15-3 based on hierarchical nanoporous PtFe alloy

Author:
Yuyang Li; Caixia Xu; He Li; Huan Wang; Dan Wu; Hongmin Ma; Yanyan Cai; Bin Du; Qin Wei
Source:
Biosensors & bioelectronics 2014 v.56 pp. 295-299
ISSN:
0956-5663
Subject:
alloys; antibodies; antigens; biocompatibility; biomarkers; detection limit; electrochemistry; graphene; hydrogen peroxide; immunoassays; immunosensors; nanomaterials; neoplasms; porous media
Abstract:
... Bimetallic hierarchical nanostructures have attracted increasing attention for biosensing applications due to their higher electrocatalytic activity and better biocompatibility. In this work, we developed a novel sandwich-type nonenzymatic electrochemical immunosensor for cancer biomarker carbohydrate antigen 15-3 (CA15-3). Hierarchical nanoporous PtFe alloys were used as carriers of the signaling ...
DOI:
10.1016/j.bios.2014.01.020

12. One-pot synthesis of high fluorescent carbon nanoparticles and their applications as probes for detection of tetracyclines

Author:
Xiaoming Yang; Yawen Luo; Shanshan Zhu; Yuanjiao Feng; Yan Zhuo; Yao Dou
Source:
Biosensors & bioelectronics 2014 v.56 pp. 6-11
ISSN:
0956-5663
Subject:
biosensors; carbon nanoparticles; cysteine; detection limit; drugs; fluorescence; humans; mixing; synthesis; tetracyclines; urine
Abstract:
... Herein, a novel strategy for synthesizing fluorescent carbon nanoparticles (CPs) with a quantum yield of approximately 7.1% has been well established by mixing l-cysteine, diphosphorus pentoxide and water. Compared with other current protocols, the method described here displayed various advantages including friendly manipulations, low cost, and rapid reactions. Subsequently, we applied the CPs pr ...
DOI:
10.1016/j.bios.2013.12.064

13. Probing phosphatase activity using redox active nanoparticles: A novel colorimetric approach for the detection of enzyme activity

Author:
Akhtar Hayat; Gonca Bulbul; Silvana Andreescu
Source:
Biosensors & bioelectronics 2014 v.56 pp. 334-339
ISSN:
0956-5663
Subject:
absorption; alkaline phosphatase; bioassays; biosensors; blood serum; catechol; ceric oxide; color; colorimetry; cost effectiveness; detection limit; dyes; enzyme activity; enzyme substrates; genes; humans; hydroquinone; nanoparticles; oligonucleotides; optical properties
Abstract:
... A new colorimetric assay for the detection of alkaline phosphatase (ALP) activity is reported based on the surface reactivity and optical properties of redox active nanoparticles of cerium oxide, or nanoceria. The method takes advantage of nanoceria color changes after interaction with products of the ALP catalyzed reaction, resulting in charge transfer complexes with very strong absorption charac ...
DOI:
10.1016/j.bios.2014.01.003

14. Quantitative electrochemical detection of cathepsin B activity in complex tissue lysates using enhanced AC voltammetry at carbon nanofiber nanoelectrode arrays

Author:
Luxi Z. Swisher; Allan M. Prior; Stephanie Shishido; Thu A. Nguyen; Duy H. Hua; Jun Li
Source:
Biosensors & bioelectronics 2014 v.56 pp. 129-136
ISSN:
0956-5663
Subject:
biosensors; blood sampling; blood serum; carbon; cathepsin B; electrochemistry; enzyme kinetics; humans; nanofibers; neoplasms; precipitin tests; proteolysis; quantitative analysis
Abstract:
... The proteolytic activity of a cancer-related enzyme cathepsin B is measured with alternating current voltammetry (ACV) using ferrocene (Fc) labeled tetrapeptides attached to nanoelectrode arrays (NEAs) fabricated with vertically aligned carbon nanofibers (VACNFs). This combination enables the use of high AC frequencies (~1kHz) with enhanced electrochemical signals. The specific proteolysis of the ...
DOI:
10.1016/j.bios.2014.01.002
PubMed:
24480132
PubMed Central:
PMC3943551

15. Real-time monitoring of cell mechanical changes induced by endothelial cell activation and their subsequent binding with leukemic cell lines

Author:
Liang Tan; Peiling Lin; Bahareh Pezeshkian; Abdul Rehman; Gerard Madlambayan; Xiangqun Zeng
Source:
Biosensors & bioelectronics 2014 v.56 pp. 151-158
ISSN:
0956-5663
Subject:
biomarkers; biosensors; endothelial cells; extinction; monitoring; neoplasm cells; physical models; quartz; tumor necrosis factor-alpha; viscoelasticity
Abstract:
... Endothelial cell (EC) activation and their subsequent binding with different cells have various mechanical consequences that, if monitored real time, can serve as a functional biomarker of many pathophysiological response mechanisms. This work presents an innovative and facile strategy to conduct such monitoring using quartz crystal microbalance (QCM), thereby relating the shifts in its frequency ...
DOI:
10.1016/j.bios.2014.01.004
PubMed:
24487102
PubMed Central:
PMC3947571

16. Robust ZnO nanoparticle embedded memory device using vancomycin conjugate and its biorecognition for electrical charging node

Author:
Minkeun Kim; Hye-Jin Lee; Sewook Oh; Yejin Kim; Hunsang Jung; Min-Kyu Oh; Yeo Joon Yoon; Tae Hyeon Yoo; Tae-Sik Yoon; Hyun Ho Lee
Source:
Biosensors & bioelectronics 2014 v.56 pp. 33-38
ISSN:
0956-5663
Subject:
bacteria; biosensors; capacitance; electronics; membrane proteins; nanoparticles; vancomycin; zinc oxide
Abstract:
... Conjugation of antibiotic vancomycin (VAN) on nanoparticles (NPs) has recently initiated novel works in the nanobiotechnology field. In this study, a bioelectronic structure using VAN conjugated zinc oxide (ZnO) NPs as charge storing elements on metal-pentacene-insulator-silicon (MPIS) device is demonstrated. Highly specific molecular recognition between the VAN and membrane protein unit mimicked ...
DOI:
10.1016/j.bios.2013.12.049

17. Sensitive SERS glucose sensing in biological media using alkyne functionalized boronic acid on planar substrates

Author:
Kien Voon Kong; Chris Jun Hui Ho; Tianxun Gong; Weber Kam On Lau; Malini Olivo
Source:
Biosensors & bioelectronics 2014 v.56 pp. 186-191
ISSN:
0956-5663
Subject:
Raman spectroscopy; alkynes; biosensors; glucose; urine
Abstract:
... In this work, we propose a novel glucose binding mechanism on a highly sensitive SERS substrate, in order to overcome challenges in specific glucose detection in bio-fluids. We make use of phenylboronic acid as a receptor for saccharide capture onto the substrate and the ability of the captured glucose molecule to undergo secondary binding with an alkyne-functionalized boronic acid to form a gluco ...
DOI:
10.1016/j.bios.2013.12.062

18. Sensitive single-color fluorescence “off–on” switch system for dsDNA detection based on quantum dots-ruthenium assembling dyads

Author:
Rui Zhang; Dongxu Zhao; Hui-Guo Ding; Yan-Xiang Huang; Hai-Zheng Zhong; Hai-Yan Xie
Source:
Biosensors & bioelectronics 2014 v.56 pp. 51-57
ISSN:
0956-5663
Subject:
Hepatitis B virus; RNA; biosensors; fluorescence; glutathione; patients; quantitative polymerase chain reaction; single-stranded DNA; wavelengths
Abstract:
... Due to the high importance of detecting DNA with both fast speed and high sensitivity, we proposed a new dsDNA detection method relying on a novel single-color fluorescence “off–on” switch system. Water-soluble glutathione capped CdTe QDs (emission at 605nm) was prepared for taking advantage of the readily tunable emission property of QDs. Initially, QDs was completely quenched by the Ru(phen)2(dp ...
DOI:
10.1016/j.bios.2013.12.059

19. Shape-dependent electron transfer kinetics and catalytic activity of NiO nanoparticles immobilized onto DNA modified electrode: Fabrication of highly sensitive enzymeless glucose sensor

Author:
Ensiyeh Sharifi; Abdollah Salimi; Esmaeil Shams; Abdollah Noorbakhsh; Mohammad K. Amini
Source:
Biosensors & bioelectronics 2014 v.56 pp. 313-319
ISSN:
0956-5663
Subject:
DNA; atomic force microscopy; biosensors; blood glucose; catalytic activity; detection limit; dielectric spectroscopy; electrochemistry; electron transfer; glassy carbon electrode; glucose; nanoparticles; nickel; oxidation
Abstract:
... Herein we describe improved electron transfer properties and catalytic activity of nickel oxide nanoparticles (NiONPs) via the electrochemical deposition on DNA modified glassy carbon electrode (DNA/GCE) surface. NiONPs deposited on the bare and DNA-coated GCE showed different morphologies, electrochemical kinetics and catalytic activities. The atomic force microscopy (AFM) images revealed the for ...
DOI:
10.1016/j.bios.2014.01.010

20. Study of glucose biosensor lifetime improvement in 37°C serum based on PANI enzyme immobilization and PLGA biodegradable membrane

Author:
Lu Fang; Bo Liang; Guang Yang; Yichuan Hu; Qin Zhu; Xuesong Ye
Source:
Biosensors & bioelectronics 2014 v.56 pp. 91-96
ISSN:
0956-5663
Subject:
biodegradability; biosensors; blood serum; cattle; electrodes; films (materials); glucose; glucose oxidase; immobilized enzymes; longevity; monitoring; nanofibers; polymers
Abstract:
... Glucose sensors with long life span were fabricated and evaluated in 37°C serum to imitate in vivo conditions. Polyaniline nanofibers were electrodeposited on sensor electrodes for excessive glucose oxidase immobilization to extend the lifetime of sensors. The sensitivity of sensors stored in 37°C bovine serum was stable for 20 days without apparent descents, and still remained 45% of its initial ...
DOI:
10.1016/j.bios.2014.01.017