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- Author:
- Lei, Jiuqian; Jing, Tao; Zhou, Tingting; Zhou, Yusun; Wu, Wei; Mei, Surong; Zhou, Yikai
- Source:
- Biosensors & bioelectronics 2014 v.54 pp. 72-77
- ISSN:
- 0956-5663
- Subject:
- antigen-antibody complex, etc ; antibodies; antigens; biosensors; blood serum; chemiluminescence; colorimetry; detection limit; energy transfer; enzyme-linked immunosorbent assay; graphene; human chorionic gonadotropin; magnetic separation; nanoparticles; peroxidase; saliva; Show all 16 Subjects
- Abstract:
- ... In this study, we report a strategy of chemiluminescence resonance energy transfer (CRET) using graphene as an efficient long-range energy acceptor. Magnetic nanoparticles were also used in CRET for simple magnetic separation and immobilization of horseradish peroxidase (HRP)-labeled anti-HCG antibody. In the design of CRET system, the sandwich-type immunocomplex was formed between human chorionic ...
- DOI:
- 10.1016/j.bios.2013.10.033
-
http://dx.doi.org/10.1016/j.bios.2013.10.033
- Author:
- Kim, Seung-Wan; Cho, Il-Hoon; Lim, Guei-Sam; Park, Gi-Na; Paek, Se-Hwan
- Source:
- Biosensors & bioelectronics 2017 v.98 pp. 7-14
- ISSN:
- 0956-5663
- Subject:
- antigen-antibody complex, etc ; C-reactive protein; antibodies; biosensors; blood serum; chromatography; color; immunoassays; lactic acid; tracer techniques; Show all 10 Subjects
- Abstract:
- ... A hybrid-biosensor system that can simultaneously fulfill the immunoassay for protein markers (e.g., C-reactive protein (CRP) and procalcitonin (PCT)) and the enzyme assay for metabolic substances (e.g., lactate) in the same sepsis-based sample has been devised. Such a challenge was pursued through the installation of an enzyme-reaction zone on the signal pad of the typical immuno-strip for the ra ...
- DOI:
- 10.1016/j.bios.2017.06.032
-
https://dx.doi.org/10.1016/j.bios.2017.06.032
- Author:
- Ramanaviciene, Almira; German, Natalija; Kausaite-Minkstimiene, Asta; Voronovic, Jaroslav; Kirlyte, Justina; Ramanavicius, Arunas
- Source:
- Biosensors & bioelectronics 2012 v.36 no.1 pp. 48-55
- ISSN:
- 0956-5663
- Subject:
- antigen-antibody complex, etc ; antibodies; chemiluminescence; detection limit; electrochemistry; human growth; hydrogen peroxide; immunoassays; immunosensors; peroxidase; physical phenomena; somatotropin; surface plasmon resonance; Show all 13 Subjects
- Abstract:
- ... An indirect immunoassay format with human growth hormone (hGH) immobilized on the self-assembled monolayer (SAM) modified surface plasmon resonance (SPR) chip has been shown to detect specific anti-hGH antibodies using the combination of three different physical phenomena in the same channel of the SPR analyzer. For the enhancement of analytical signal and sensitivity of the immunosensor horseradi ...
- DOI:
- 10.1016/j.bios.2012.03.036
-
http://dx.doi.org/10.1016/j.bios.2012.03.036
- Author:
- Vasudev, Abhay; Kaushik, Ajeet; Bhansali, Shekhar
- Source:
- Biosensors & bioelectronics 2013 v.39 pp. 300-305
- ISSN:
- 0956-5663
- Subject:
- antigen-antibody complex, etc ; antibodies; detection limit; electrochemistry; electrodes; electron transfer; epidermal growth factor receptors; gold; immunosensors; propionic acid; Show all 10 Subjects
- Abstract:
- ... This paper presents an electrochemical immune sensor for label free detection of epidermal growth factor receptor (EGFR) by immobilizing anti-EGFR antibody (Anti-EGFRab) on dithiobissuccinimidyl propionate (DTSP) self-assembled monolayer (SAM) on gold (Au) electrode. Electrochemical studies show that increased surface concentration of redox moieties onto Anti-EGFRab/DTSP immuno-electrode leads to ...
- DOI:
- 10.1016/j.bios.2012.06.012
-
http://dx.doi.org/10.1016/j.bios.2012.06.012
- Author:
- Zhu, Yingyue; Xu, Liguang; Ma, Wei; Chen, Wei; Yan, Wenjing; Kuang, Hua; Wang, Libing; Xu, Chuanlai
- Source:
- Biosensors & bioelectronics 2011 v.26 no.11 pp. 4393-4398
- ISSN:
- 0956-5663
- Subject:
- antigen-antibody complex, etc ; World Health Organization; antibodies; antigens; colorimetry; detection limit; drinking water; immunoassays; immunosensors; microcystin-LR; nanogold; peroxidase; screening; Show all 13 Subjects
- Abstract:
- ... In this paper, we demonstrate the application of versatile G-quadruplex–hemin DNAzymes in an immunoassay for detecting Microcystin-LR (MC-LR). Taking advantage of the high peroxidase activity of G-quadruplex–hemin complexes and the enhancement effect of gold nanoparticles (AuNPs), the method showed simple, high sensitive and selectivity detection of target toxin residues in water samples. The coat ...
- DOI:
- 10.1016/j.bios.2011.04.047
-
http://dx.doi.org/10.1016/j.bios.2011.04.047
- Author:
- Guo, Zhiyong; Hao, Tingting; Du, Shuping; Chen, Beibei; Wang, Zebo; Li, Xing; Wang, Sui
- Source:
- Biosensors & bioelectronics 2013 v.44 pp. 101-107
- ISSN:
- 0956-5663
- Subject:
- antigen-antibody complex, etc ; antibodies; antigens; biosensors; cross reaction; detection limit; electrodes; electron transfer; glass; graphene; immunoassays; monitoring; quantum dots; Show all 13 Subjects
- Abstract:
- ... A multiplex electrochemiluminescence (ECL) immunoassay for simultaneous determination of two different tumor markers, alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA), using multicolor quantum dots as labels and graphene as conducting bridge was developed. Herein, a typical sandwich immune complex was constructed on the glass carbon electrode, with QDs525 and QDs625 labeled on secondary ...
- DOI:
- 10.1016/j.bios.2013.01.025
-
http://dx.doi.org/10.1016/j.bios.2013.01.025
- Author:
- Wu, Haiyun; Ohnuki, Hitoshi; Ota, Shirei; Murata, Masataka; Yoshiura, Yasutoshi; Endo, Hideaki
- Source:
- Biosensors & bioelectronics 2017 v.93 pp. 57-64
- ISSN:
- 0956-5663
- Subject:
- antigen-antibody complex, etc ; Oreochromis niloticus; antibodies; cortisol; electrochemistry; electrodes; enzyme-linked immunosorbent assay; fish; fish farms; glucose; glucose oxidase; gold; immunosensors; monitoring; oxidation; rapid methods; stress response; Show all 17 Subjects
- Abstract:
- ... Fishes display a wide variation in their physiological responses to stress, which is clearly evident in the plasma corticosteroid changes, chiefly cortisol levels in fish. As a well-known indicator of fish stress, a simple and rapid method for detecting cortisol changes especially sudden increases is desired. In this study, we describe an enzyme-functionalized label-free immunosensor system for de ...
- DOI:
- 10.1016/j.bios.2016.10.001
-
https://dx.doi.org/10.1016/j.bios.2016.10.001
- Author:
- Holford, Timothy R.J.; Davis, Frank; Higson, Séamus P.J.
- Source:
- Biosensors & bioelectronics 2012 v.34 no.1 pp. 12-24
- ISSN:
- 0956-5663
- Subject:
- antigen-antibody complex, etc ; antibodies; automation; electrochemistry; immunoassays; immunosensors; monitoring; Show all 7 Subjects
- Abstract:
- ... This review details recent advances in the fields of immunosensors and closely related immunoassays in the past decade, together with a discussion of possible future trends. Immunosensors can be classified by the way in which they transduce the signal produced upon the formation of an antibody antigen complex. Recent advancements to these methods of detection and transduction are discussed in deta ...
- DOI:
- 10.1016/j.bios.2011.10.023
-
http://dx.doi.org/10.1016/j.bios.2011.10.023
- Author:
- Wang, Hao; Zhang, Bihong; Xi, Jiajia; Zhao, Faqiong; Zeng, Baizhao
- Source:
- Biosensors & bioelectronics 2019 v.141 pp. 111443
- ISSN:
- 0956-5663
- Subject:
- antigen-antibody complex, etc ; antibodies; antigens; chemical bonding; detection limit; electric current; hydrogen peroxide; immunoassays; immunosensors; oxygen; photocatalysts; Show all 11 Subjects
- Abstract:
- ... A novel label-free photocathodic immunosensor was constructed by introducing a direct Z-scheme I-BiOCl/CdS cathodic material as highly effective photocatalyst for the selective detection of carcino embryonic antigen (CEA). The Z-scheme photocatalyst could promote the separation of photogenerated carriers and showed a more negative conduction band potential. In addition, the I-BiOCl with abundant o ...
- DOI:
- 10.1016/j.bios.2019.111443
-
https://dx.doi.org/10.1016/j.bios.2019.111443