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1. Absolute quantification and analysis of extracellular vesicle lncRNAs from the peripheral blood of patients with lung cancer based on multi-colour fluorescence chip-based digital PCR

Author:
Lou, Jiatao,   et al.   ; Bai, Yanan; Qu, Youlan; Wu, Zhenhua; Ren, Yijiu; Cheng, Zule; Lu, Yunxing; Hu, Jie; Zhao, Jianlong; Chen, Chang; Mao, Hongju;     Show all 11 Authors
Source:
Biosensors & bioelectronics 2019 v.142 pp. 111523
ISSN:
0956-5663
Subject:
RNA; biomarkers; biopsy; biosensors; blood; detection limit; early diagnosis; genes; lung neoplasms; lungs; patients; quantitative polymerase chain reaction; t-test
Abstract:
... Emerging evidence indicates that extracellular vesicle (EV) long non-coding ribonucleic acids (lncRNAs) in lung cancer may be clinically useful biomarkers for early diagnosis using liquid biopsy. However, the extremely low quantities of EV-lncRNAs in peripheral blood are a major challenge for multi-target detection. In this study, we developed a new multi-colour fluorescence digital PCR EV-lncRNA ...
DOI:
10.1016/j.bios.2019.111523