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- Wang, Ying; Li, Guo-Si; Qiao, Pei; Lin, Ling; Xue, Hai-Long; Zhu, Li; Wu, Mian-Bin; Lin, Jian-Ping; Yang, Li-Rong
- Biotechnology letters 2018 v.40 no.11-12 pp. 1551-1559
- Bacillus sphaericus; Candida boidinii; Escherichia coli K12; NAD (coenzyme); biosynthesis; directed evolution; enzyme activity; formate dehydrogenase; half life; heat treatment; leucine; mutants; temperature; thermal stability; threonine
- ... OBJECTIVE: To strengthen NADH regeneration in the biosynthesis of L-2-aminobutyric acid (L-ABA). RESULTS: L-Threonine deaminase (L-TD) from Escherichia coli K12 was modified by directed evolution and rational design to improve its endurance to heat treatment. The half-life of mutant G323D/F510L/T344A at 42 °C increased from 10 to 210 min, a 20-fold increase compared to the wild-type L-TD, and the ...