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- Zhang, Rongguang; Duan, Guangcai; Shi, Qingfeng; Chen, Shuaiyin; Fan, Qingtang; Sun, Nan; Xi, Yuanlin
- Biotechnology letters 2016 v.38 no.11 pp. 1911-1916
- Escherichia coli; Helicobacter pylori; Lactococcus lactis; Western blotting; antigens; edible vaccines; gene fusion; humoral immunity; immune response; mice; molecular weight; nisin; oral vaccination; plasmids; vaccine development
- ... OBJECTIVES: To develop orally administrated anti-Helicobacter pylori vaccination, a Lactococcus lactis strain was genetically constructed for fusion expression of H. pylori protective antigens HpaA and Omp22. RESULTS: The fusion gene of omp22 and hpaA with an adapter encoding three glycines was cloned from a plasmid pMAL-c2x-omp22-hpaA into Escherichia coli MC1061 and L. lactis NZ3900 successively ...