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You searched for: Journal Biotechnology letters Remove constraint Journal: Biotechnology letters Publication Year 2008 Remove constraint Publication Year: 2008 Source 2008 v.30 no.6 Remove constraint Source: 2008 v.30 no.6
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1. Fatty acid unsaturation, mobilization, and regulation in the response of plants to stress

Author:
Upchurch, Robert G.
Source:
Biotechnology letters 2008 v.30 no.6 pp. 967
ISSN:
0141-5492
Subject:
unsaturated fatty acids; abiotic stress; plant stress; plant pathogens
Abstract:
... Stress acclimating plants respond to abiotic and biotic stress by remodeling membrane fluidity and by releasing α-linolenic (18:3) from membrane lipids. The modification of membrane fluidity is mediated by changes in unsaturated fatty acid levels, a function provided in part by the regulated activity of fatty acid desaturases. Adjustment of membrane fluidity maintains an environment suitable for t ...
Handle:
10113/15027
DOI:
10.1007/s10529-008-9639-z
PubMed:
18227974

2. Property control of sophorolipids: influence of fatty acid substrate and blending

Author:
Ashby, Richard D.; Solaiman, Daniel K. Y.; Foglia, Thomas A.
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1093
ISSN:
0141-5492
Subject:
lipids; Candida; batch fermentation; glucose; fatty acids; palmitic acid; stearic acid; oleic acid; linoleic acid; mass spectrometry; surfactants; surface active properties
Abstract:
... Sophorolipids (SLs) were synthesized by fed-batch fermentation of Candida bombicola on glucose and either palmitic acid (SL-p), stearic acid (SL-s), oleic acid (SL-o) or linoleic acid (SL-l) and the structural distribution accurately determined by atmospheric pressure chemical ionization-mass spectrometry (APCI-MS). The surfactant properties, including critical micelle concentration (CMC), minimum ...
Handle:
10113/31988
DOI:
10.1007/s10529-008-9653-1
PubMed:
18264681

3. A chemically modified glass surface that facilitates transglutaminase-mediated protein immobilization

Author:
Tanaka, Yusuke; Doi, Satoshi; Kamiya, Noriho; Kawata, Noriyuki; Kamiya, Shinji; Nakama, Kenichi; Goto, Masahiro
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1025-1029
ISSN:
0141-5492
Subject:
glass; green fluorescent protein; protein-glutamine gamma-glutamyltransferase
Abstract:
... An amino-modified glass surface for enzymatic protein immobilization by microbial transglutaminase (MTG) was developed. Diamine substrates with secondary amino groups in the linker moiety, like triethylenetetramine (TETA), exhibited at most a 2-fold higher reactivity in the MTG-catalyzed reaction compared to those with the alkyl linker. A 96-well glass plate was subsequently modified with selected ...
DOI:
10.1007/s10529-008-9656-y
PubMed:
18259875

4. A modified DNA isolation protocol for obtaining pure RT-PCR grade RNA

Author:
Klassen, Roland; Fricke, Julia; Pfeiffer, Annika; Meinhardt, Friedhelm
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1041-1044
ISSN:
0141-5492
Subject:
Saccharomyces cerevisiae; complementary DNA; genes; messenger RNA; molecular weight; phenol; ribosomal RNA; transfer RNA
Abstract:
... We provide a simple but very efficient method for RNA preparation from Saccharomyces cerevisiae based on a standard chromosomal DNA isolation protocol. The method yields DNA-free total RNA, including mRNA, rRNA, and tRNA but can easily be adjusted to considerably enrich low molecular weight RNAs, such as tRNAs and the small rRNA species (5S and 5.8S). The procedure was proven and validated by veri ...
DOI:
10.1007/s10529-008-9648-y
PubMed:
18246302

5. A novel configuration of microbial fuel cell stack bridged internally through an extra cation exchange membrane

Author:
Liu, Zhidan; Liu, Jing; Zhang, Songping; Su, Zhiguo
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1017-1023
ISSN:
0141-5492
Subject:
cation exchange; cations; chemical oxygen demand; electrodes; fuels; microbial fuel cells
Abstract:
... This paper reports a novel configuration of stacked microbial fuel cells (MFCs) bridged internally through an extra cation exchange membrane (CEM). The MFC stack (MFCstack), assembled from two single MFCs (MFCsingle), resulted in double voltage output and half optimal external resistance. COD removal rate was increased from 32.4% to 54.5%. The performance improvement could be attributed to the sma ...
DOI:
10.1007/s10529-008-9658-9
PubMed:
18259873

6. A novel nanohybrid of daunomycin and single-walled carbon nanotubes: photophysical properties and enhanced electrochemical activity

Author:
Lu, Yanhong; Yang, Xiaoying; Ma, Yanfeng; Huang, Yi; Chen, Yongsheng
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1031-1035
ISSN:
0141-5492
Subject:
carbon; carbon nanotubes; dyes; electrochemistry; fluorescence; spectroscopy
Abstract:
... A nanohybrid adduct of the widely used, functional dye, daunomycin (DM), with single-walled carbon nanotubes (SWNTs) was prepared. Ultraviolet-visible-near infrared and fluorescence spectroscopy and electrochemistry of DM-functionalized SWNTs reveal that DM interacts with SWNTs through strong π-π stacking and there is a significant photo-induced charge-transfer interaction between the two componen ...
DOI:
10.1007/s10529-008-9641-5
PubMed:
18224281

7. A novel two-step fermentation process for improved arachidonic acid production by Mortierella alpina

Author:
Jin, Ming-Jie; Huang, He; Xiao, Ai-Hua; Zhang, Kun; Liu, Xin; Li, Shuang; Peng, Chao
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1087-1091
ISSN:
0141-5492
Subject:
Mortierella alpina; aeration; agitation; arachidonic acid; ethanol; fermentation; fermenters; mycelium
Abstract:
... A novel two-step fermentation process was developed to enhance arachidonic acid (ARA) production by Mortierella alpina ME-1 in a 5 l fermentor. Agitation speed and aeration rate were adjusted from 180 to 40 rpm and from 0.6 to1 vvm, respectively, after 5 days cultivation, to decrease physical damage to the mycelia and to extend the stationary phase. Moreover, 3% (w/v) and 2% (w/v) ethanol were fed ...
DOI:
10.1007/s10529-008-9661-1
PubMed:
18256790

8. A screening system for inhibitors of trichothecene biosynthesis: hydroxylation of trichodiene as a target

Author:
Takahashi-Ando, Naoko; Ochiai, Noriyuki; Tokai, Takeshi; Ohsato, Shuichi; Nishiuchi, Takumi; Yoshida, Minoru; Fujimura, Makoto; Kimura, Makoto
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1055-1059
ISSN:
0141-5492
Subject:
screening; Fusarium graminearum; biosynthesis; flavones; yeasts; Saccharomyces cerevisiae; high performance liquid chromatography; cytochrome P-450; trichothecenes; hydroxylation; catechin; beverages
Abstract:
... Fusarium Tri4 encodes a key cytochrome P450 monooxygenase for hydroxylation of trichodiene early in the biosynthesis of trichothecenes. In this study, we established a system for screening for inhibitors of trichothecene biosynthesis using transgenic Saccharomyces cerevisiae expressing Tri4. For easy evaluation of the TRI4 activity, trichodiene-11-one was used as a substrate and the formation of 2 ...
DOI:
10.1007/s10529-008-9649-x
PubMed:
18239857

9. Biokinetic parameters and behavior of Aeromonas hydrophila during anaerobic growth

Author:
Lee, Seungyeop; Kim, Jaai; Shin, Seung Gu; Hwang, Seokhwan
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1011-1016
ISSN:
0141-5492
Subject:
Aeromonas hydrophila; DNA; acetates; ethanol; glucose; metabolism; risk; succinic acid; washing
Abstract:
... The biokinetics of glucose metabolism were evaluated in Aeromonas hydrophila during growth in an anaerobic biosystem. After approx 34 h growth, A. hydrophila metabolized 5,000 mg glucose l⁻¹ into the end-products ethanol, acetate, succinate and formate. The maximum growth rate, μ m, half saturation coefficients, K s, microbial yield coefficient, Y, cell mass decay rate coefficient, k d, and substr ...
DOI:
10.1007/s10529-008-9660-2

10. Changes in the activity of the multifunctional β-glycosyl hydrolase (Cel44C-Man26A) from Paenibacillus polymyxa by removal of the C-terminal region to minimum size

Author:
Cho, Kye-Man; Math, Renukaradhya K.; Hong, Su-Young; Asraful Islam, Shah Md.; Kim, Jong-Ok; Hong, Sun-Joo; Kim, Hoon; Yun, Han-Dae
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1061-1068
ISSN:
0141-5492
Subject:
Paenibacillus polymyxa; active sites; amino acids; beta-mannosidase; endo-1,4-beta-glucanase; enzyme activity; fibronectins; licheninase; pH; xylanases
Abstract:
... Paenibacillus polymyxa GS01 secretes Cel44C-Man26A as a multifunctional enzyme with cellulase, xylanase, lichenase, and mannanase activities. Cel44C-Man26A consists of 1,352 amino acids in which present a catalytic domain (CD) of the glycosyl hydrolase family 44 (GH44), fibronectin domain type 3 (Fn3), catalytic domain of glycosyl hydrolase family 26 (GH26), and a cellulose-binding module type 3 ( ...
DOI:
10.1007/s10529-008-9640-6

11. Combinational biosynthesis of a fluorescent cyanobacterial holo-α-allophycocyanin in Escherichia coli

Author:
Yang, Yu; Ge, Baosheng; Guan, Xiangyu; Zhang, Weijie; Qin, Song
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1001-1004
ISSN:
0141-5492
Subject:
Escherichia coli; biosynthesis; gene overexpression; genes
Abstract:
... Allophycocyanin (APC) is a phycobiliprotein with various biological and pharmacological properties. An expression vector containing five essential genes in charge of biosynthesis of cyanobacterial APC holo-α subunit (holo-ApcA) was constructed, resulting in over-expression of a fluorescent holo-ApcA in E. coli. After being cultured for 16 h, the dry cell density reached 22.5 g l⁻¹, and the express ...
DOI:
10.1007/s10529-008-9644-2

12. Designing a whole-cell biotransformation system in Escherichia coli using cytochrome P450 from Streptomyces peucetius

Author:
Shrestha, Pramod; Oh, Tae-Jin; Sohng, Jae Kyung
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1101-1106
ISSN:
0141-5492
Subject:
Escherichia coli; Pseudomonas putida; Streptomyces peucetius; biotransformation; cytochrome P-450; plasmids
Abstract:
... A biotransformation system was designed to co-express CYP107P3 (CSP4), cytochrome P450, from Streptomyces peuceticus, along with CamA (putidaredoxin reductase) and CamB (putidaredoxin) from Pseudomonas putida, the necessary reducing equivalents, in a class I type electron-transfer system in E. coli BL21 (DE3). This was carried out using two plasmids with different selection markers and compatible ...
DOI:
10.1007/s10529-008-9654-0
PubMed:
18259876

13. Enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media

Author:
Kamiya, Noriho; Matsushita, Yuichi; Hanaki, Misa; Nakashima, Kazunori; Narita, Mamiko; Goto, Masahiro; Takahashi, Haruo
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1037-1040
ISSN:
0141-5492
Subject:
cellulose; citrates; endo-1,4-beta-glucanase; enzyme activity; glucose; ionic liquids; mixing; pH; saccharification
Abstract:
... The enzymatic saccharification of a model cellulosic substrate, Avicel PH-101, using an ionic liquid (IL), 1-ethyl-3-methylimidazolium diethylphosphate, was explored. After mixing the IL solution of cellulose with different volumes of 10 mM citrate buffer (pH 5.0), cellulase was directly added to the aqueous-IL mixture at 40°C. When the volume of IL to water was greater than 3:2, little cellulase ...
DOI:
10.1007/s10529-008-9638-0
PubMed:
18250967

14. Expression, purification and characterization of anti-BAFF antibody secreted from the yeast Pichia pastoris

Author:
Ren, Fang; Li, Bao-Cun; Zhang, Na-Na; Cao, Meng; Dan, Wen-Bing; Zhang, Shuang-Quan
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1075-1080
ISSN:
0141-5492
Subject:
cell viability; Komagataella pastoris; yeasts; genes; autoimmunity
Abstract:
... B-Cell activating factor (BAFF) is critical for B cell survival and maturation; excessive expression of it corrupts B-cell tolerance and may lead to autoimmunity. The gene, scFv-Fc, coding for the antibody of BAFF was inserted into the eukaryotic expression vector, pPICZαA, and transformed into Pichia pastoris. A high-level expression strain was obtained using a 'yeastern blotting' method. The scF ...
DOI:
10.1007/s10529-008-9657-x

15. Hexavalent chromate reduction by immobilized Streptomyces griseus

Author:
Poopal, Ashwini C.; Laxman, R. Seeta
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1005-1010
ISSN:
0141-5492
Subject:
Streptomyces griseus; chromium; copper; immobilized cells; magnesium; manganese; mutagenicity; zinc
Abstract:
... Hexavalent chromium, which is a mutagen and carcinogen, was efficiently reduced by Streptomyces griseus. This activity was associated with the cell. Cr⁶⁺ reduction by free as well as immobilized cells was studied: cells in PVA-alginate had the highest (100%) Cr⁶⁺ removal efficiency in 24 h with reduction rates similar to free cells. Immobilized cells completely reduced 25 mg Cr⁶⁺ l⁻¹ in 24 h. PVA- ...
DOI:
10.1007/s10529-008-9662-0
PubMed:
18259872

16. Metabolic alternations in SOD-deficient Escherichia coli cells when cultivated under oxidative stress from photoexcited titanium dioxide

Author:
Ojima, Yoshihiro; Nishioka, Motomu; Taya, Masahito
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1107-1113
ISSN:
0141-5492
Subject:
Escherichia coli; acetate kinase; acetic acid; biochemical pathways; citrate (si)-synthase; gene expression; messenger RNA; oxidative stress; reactive oxygen species; superoxide dismutase; titanium dioxide
Abstract:
... Superoxide dismutase (SOD)-deficient Escherichia coli was cultivated under the oxidative stress generated by photoexcited titanium dioxide. These cells showed higher growth rate and glucose consumption rate with accelerated accumulation of acetic acid in the medium, compared to the cells cultivated under the normal condition without the stress. Under the stress condition, the activity of acetate k ...
DOI:
10.1007/s10529-008-9655-z

17. Morphological observation of nuclear sub-localization of human angiogenin in HUVECs

Author:
Wu, Jie; Zhang, Hong-Bin; Wang, Jie; Yang, Tai-Cheng; Xian, Jiang; Yang, Chuan-Hong; Lai, Huang-Wen
Source:
Biotechnology letters 2008 v.30 no.6 pp. 989-993
ISSN:
0141-5492
Subject:
microscopy; hybridomas; immunoblotting; humans; enzyme-linked immunosorbent assay; gel chromatography; Escherichia coli
Abstract:
... Angiogenin, a potent angiogenic factor, was cloned and expressed by Escherichia coli and then purified with gel filtration chromatography. Approximately 90% pure angiogenin was obtained to generate a monoclonal antibody. Using western immunoblotting and ELISA, we confirmed that monoclonal antibody C46 secreted from hybridoma cells stably and specifically binds to angiogenin. The fused protein angi ...
DOI:
10.1007/s10529-008-9651-3
PubMed:
18246300

18. Production, characterization and (co-)immobilization of dextranase from Penicillium aculeatum

Author:
Erhardt, Frank A.; Stammen, Simon; Jördening, Hans-Joachim
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1069-1073
ISSN:
0141-5492
Subject:
Penicillium; activation energy; adsorption; bentonite; dextranase; dextransucrase; fermentation; hydrogen peroxide; isomaltose; isozymes; microbial growth; mycelium; pretreatment; sucrose
Abstract:
... Fermentation kinetics of Penicillium aculeatum ATCC 10409 demonstrated that fungal growth and dextranase release are decoupled. Inoculation by conidia or mycelia resulted in identical kinetics. Two new isoenzymes of the dextranase were characterized regarding their kinetic constants, pI, MW, activation energy and stabilities. The larger enzyme was 3-fold more active (turnover number: 2,230 ± 97 s⁻ ...
DOI:
10.1007/s10529-008-9659-8
PubMed:
18264680

19. Quantification of kinetics for enzyme-catalysed reactions: implications for diffusional limitations at the 10 ml scale

Author:
Matosevic, S.; Micheletti, M.; Woodley, J. M.; Lye, G. J.; Baganz, F.
Source:
Biotechnology letters 2008 v.30 no.6 pp. 995-1000
ISSN:
0141-5492
Subject:
enzymatic reactions; enzyme kinetics; mixing; models; statistical analysis; transketolase
Abstract:
... The effects of different reaction scales [100 μl reactions in 96-standard round well (SRW) plates and 10 ml reactions in 24-square well (SW) plates] have been investigated using, as a model, transketolase (TK)-catalysed reaction producing l-erythrulose. Reactions were carried out under non-shaking, shaking and at 10 ml scale stirring conditions to assess the effect of diffusional limitations. Stat ...
DOI:
10.1007/s10529-008-9645-1
PubMed:
18224278

20. Single bacterial cell detection using a mutant luciferase

Author:
Noda, Kenichi; Matsuno, Tadahiro; Fujii, Hiroya; Kogure, Tomonari; Urata, Masaaki; Asami, Yasuo; Kuroda, Akio
Source:
Biotechnology letters 2008 v.30 no.6 pp. 1051-1054
ISSN:
0141-5492
Subject:
Bacillus subtilis; Escherichia coli; Photinus pyralis; adenosine triphosphate; bacterial contamination; light intensity; luciferase
Abstract:
... Previously, we constructed a genetically modified luciferase of Photinus pyralis that generates more than 10-fold higher luminescence intensity than the wild-type enzyme. In this study, we demonstrate that this modified luciferase enables us to detect ATP at 10⁻¹⁸ mol, which is almost equal to the quantity contained in a single bacterial cell. Consequently,we have been able to detect bacterial con ...
DOI:
10.1007/s10529-008-9643-3