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You searched for: Journal Biotechnology letters Remove constraint Journal: Biotechnology letters Publication Year 2004 Remove constraint Publication Year: 2004 Source 2004 v.26 no.12 Remove constraint Source: 2004 v.26 no.12
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1. Development of an optical immunosensor based on the fluorescence of Cyanine-5 for veterinarian diagnostics

Author:
Silva, M.; Cruz, H.; Rossetti, O.; Arese, A.; Oliva, A.
Source:
Biotechnology letters 2004 v.26 no.12 pp. 993-997
ISSN:
0141-5492
Subject:
biosensors; diagnostic techniques; antibody detection; Brucella; animal pathogenic bacteria; sheep; infection; veterinary medicine
Abstract:
... A model optical immunosensor was developed to quantify an antibody present in a sample by measuring the fluorescence of Cyanine-5 conjugated with the antibody, using a competitive and a sandwich immunoreaction configuration, with the antigen immobilised in controlled pore glass beads. At pH 2, 94% of the antigen-antibody complex was dissociated, allowing reutilisation. Photobleaching had no effect ...
DOI:
10.1023/B:BILE.0000030046.96039.e8
PubMed:
15269526

2. Expression of a human lactoferrin N-lobe in Nicotiana benthmiana with potato virus X-based agroinfection

Author:
Li, Y.; Geng, Y.; Song, H.; Zheng, G.; Huan, L.; Qiu, B.
Source:
Biotechnology letters 2004 v.26 no.12 pp. 953-957
ISSN:
0141-5492
Subject:
Nicotiana benthamiana; Potato virus X; plasmid vectors; Agrobacterium tumefaciens; lactoferrin; humans; recombinant proteins; antibacterial properties; molecular cloning; biotechnology; genetically modified organisms
Abstract:
... A DNA fragment encoding the N-terminal half (N-lobe) of the human lactoferrin (hLfN) gene has now been cloned into recombinant Potexvirus potato virus X (PVX) vector and expressed in Nicotiana benthmiana using agroinfection. Western blot analysis showed the recombinant protein with an apparent molecular mass on electrophoresis of ca. 40 kDa, corresponding to the predicted size of the hLfN. The yie ...
DOI:
10.1023/B:BILE.0000030038.27358.20
PubMed:
15269518

3. Quantification of Saccharomyces cerevisiae viability using BacLight

Author:
Zhang, T.; Fang, H.H.P.
Source:
Biotechnology letters 2004 v.26 no.12 pp. 989-992
ISSN:
0141-5492
Subject:
yeasts; viability; differential staining; quantitative analysis; analytical kits; confocal scanning laser microscopy; fluorescence; microscopy; flow cytometry
Abstract:
... Yeast viability can be accurately quantified using BacLight, a kit which so far has been used only for bacterial analysis. Upon staining, viable cells can be differentiated from non-viable ones by either confocal laser scanning microscopy (CLSM), epifluorescence microscopy, or flow cytometry. Using Saccharomyces cerevisiae as a model, viabilities quantified by CLSM deviated an average of 1.7% from ...
DOI:
10.1023/B:BILE.0000030045.16713.19
PubMed:
15269525

4. Reverse transcription quantitative-PCR of three genes with high homology encoding 3-hydroxy-methylglutaryl-CoA reductase in rice

Author:
Kim, B.R.; Nam, H.Y.; Kim, S.U.; Pai, T.; Chang, Y.J.
Source:
Biotechnology letters 2004 v.26 no.12 pp. 985-988
ISSN:
0141-5492
Subject:
Oryza sativa; seedlings; etiolation; hydroxymethylglutaryl-CoA reductase; multigene family; sequence homology; gene expression; polymerase chain reaction; reverse transcription; plant genetics
Abstract:
... Reverse transcription followed by RT Q-PCR is useful for the systematic measurement of changes in gene expression. RT Q-PCR with two pairs of primers for each gene was used for relative expression of three genes with high homology encoding 3-hydroxy-methylglutaryl-CoA reductase (HMGR) in rice. At various growth stages of etiolated seedling and various times after UV-irradiation treatment, RT Q-PCR ...
DOI:
10.1023/B:BILE.0000030044.79075.9b
PubMed:
15269524