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1. Anthelmintic residues in goat and sheep dairy products

Author:
Jedziniak, Piotr; Olejnik, Małgorzata; Rola, Jolanta G.; Szprengier-Juszkiewicz, Teresa
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 515-518
ISSN:
2300-3235
Subject:
abamectin; albendazole; cambendazole; clorsulon; closantel; cottage cheese; cream cheese; dairy sheep; doramectin; eprinomectin; ewe milk; flubendazole; goats; imidocarb; ioxynil; ivermectin; markets; mebendazole; metabolites; milk; morantel; moxidectin; multiresidue analysis; niclosamide; nitroxinil; oxibendazole; praziquantel; pyrantel; thiabendazole; triclabendazole; yogurt
Abstract:
... A multiresidue method (LC-MS/MS) for determination of wide range of anthelmintics was developed. The method covered benzimidazoles: albendazole (and metabolites), cambendazole, fenbendazol (and metabolites), flubendazole (and metabolites), mebendazole (and metabolites), oxibendazole, thiabendazole (and metabolites), triclabendazole (and metabolites); macrocyclic lactones: abamectin, doramectin, em ...
DOI:
10.1515/bvip-2015-0077

2. Application of SYBR Green I and TaqMan probe-based real-time PCRs for the identification of Listeria spp. and Listeria monocytogenes

Author:
Kędrak-Jabłońska, Agnieszka; Budniak, Sylwia; Szczawińska, Anna; Reksa, Monika; Krupa, Marek; Szulowski, Krzysztof
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 489-494
ISSN:
2300-3235
Subject:
DNA probes; Listeria grayi; Listeria innocua; Listeria ivanovii; Listeria monocytogenes; Listeria seeligeri; Listeria welshimeri; bacteria; detection limit; fluorescence; genes; pathogen identification; quantitative polymerase chain reaction; ribosomal DNA
Abstract:
... The aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene of Listeria spp. and the hlyA gene of Listeria monocytogenes. Five strains of L. monocytogenes and single strains of each of the species: L. ivanovii, L. innocua, L. grayi, L. welshimeri, and L. seelige ...
DOI:
10.1515/bvip-2015-0073

3. Avian tuberculosis in a captive cassowary (Casuarius casuarius)

Author:
Krajewska, Monika; Czujkowska, Agnieszka; Weiner, Marcin; Lipiec, Marek; Szulowski, Krzysztof
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 483-488
ISSN:
2300-3235
Subject:
Mycobacterium avium subsp. avium; animal age; animal breeding; animal pens; avian tuberculosis; body size; captive animals; cassowaries; euthanasia; in vitro culture; liver; spleen; zoos; Netherlands
Abstract:
... The paper describes avian tuberculosis in a captive bred cassowary. A two-and-a-half-year-old bird was obtained by a Polish zoo in 2010 from the Netherlands under conditions compliant with the recommendations of the European Association of Zoos and Aquaria. Despite being of small size for the age, the bird appeared healthy and showed no signs of the disease until the day when it was found recumben ...
DOI:
10.1515/bvip-2015-0072

4. Characteristics, immunological events, and diagnostics of Babesia spp. infection, with emphasis on Babesia canis

Author:
Kostro, Krzysztof; Stojecki, Krzysztof; Grzybek, Maciej; Tomczuk, Krzysztof
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 495-504
ISSN:
2300-3235
Subject:
Babesia canis; babesiosis; diagnostic techniques; disease course; disease diagnosis; disease vectors; dogs; immunology; prediction; therapeutics; ticks; veterinarians; veterinary parasitology; Poland
Abstract:
... Vector-borne infection constitutes a significant health issue in dogs worldwide. Recent reports point to an increasing number of canine vector-borne disease cases in European countries, including Poland. Canine babesiosis caused by various Babesia species is a protozoal tick-borne disease with worldwide distribution and significant veterinary importance. The development and application of molecula ...
DOI:
10.1515/bvip-2015-0074

5. Different pattern of haemagglutinin immunoreactivity of equine influenza virus strains isolated in Poland

Author:
Kwaśnik, Małgorzata; Góra, Ilona Marcelina; Rożek, Wojciech
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 463-466
ISSN:
2300-3235
Subject:
Influenza A virus; amino acid sequences; antigens; hemagglutinins; immune response; monoclonal antibodies; phylogeny; point mutation; polypeptides; Florida; Poland
Abstract:
... The immunoreactivity of haemagglutinin (HA) polypeptides of equine influenza virus was compared among the strains isolated in Poland, using H3 monoclonal antibody. A stronger signal in immunoblot reaction was observed for A/equi/Pulawy/2008 HA polypeptides compared to A/equi/Pulawy/2006, despite the fact that both strains are phylogenetically closely related and belong to Florida clade 2 of Americ ...
DOI:
10.1515/bvip-2015-0068

6. Dioxins, furans, and polychlorinated biphenyls in hen eggs – a new source of hazard for consumers?

Author:
Piskorska-Pliszczyńska, Jadwiga; Struciński, Paweł; Mikołajczyk, Szczepan; Maszewski , Sebastian; Rachubik, Jarosław; Warenik-Bany, Małgorzata
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 519-526
ISSN:
2300-3235
Subject:
organic foods; risk; hens; at-risk population; normal values; meat; furans; polychlorinated biphenyls; soil; solid wastes; polychlorinated dibenzodioxins; polychlorinated dibenzofurans; eggs
Abstract:
... The paper presents the results of testing eggs for the content of dioxins and polychlorinated biphenyls (PCBs), determination of the sources that caused the presence of high concentrations of these compounds which exceeded the acceptable contamination levels, and assessment of consumer health risk caused by the consumption of eggs with excessive contents of investigated compounds. In about 9% of f ...
DOI:
10.1515/bvip-2015-0078

7. Effect of HMB and 2-Ox administered during pregnancy on bone properties in primiparous and multiparous minks (Neivison vison)

Author:
Tomaszewska, Ewa; Dobrowolski, Piotr; Kostro, Krzysztof; Jakubczak, Andrzej; Taszkun, Iwona; Jaworska-Adamu, Jadwiga; Żmuda, Andrzej; Rycerz, Karol; Muszyński, Siemowit
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 563-568
ISSN:
2300-3235
Subject:
bone density; diet; humerus; lactation; metabolites; milk production; mink; nutrient deficiencies; oral administration; pregnancy; reproductive performance
Abstract:
... The aim of the study was to determine the mechanical and geometric properties as well as bone tissue density of long bones in primiparous and multiparous dams of minks supplemented with β-hydroxy β-methylbutyrate (HMB) and/or 2-oxoketoglutarate (2-Ox) during gestation. Powdered 2-Ox was given at the daily dosage of 0.4 g/kg b.w. separately or simultaneously with HMB, which was administered at the ...
DOI:
10.1515/bvip-2015-0084

8. Efficiency of modified therapeutic protocol in the treatment of some varieties of canine cardiovascular dirofilariasis

Author:
Stepanović,; Ilić, Tamara; Krstić, Nikola; Dimitrijević, Sanda
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 505-510
ISSN:
2300-3235
Subject:
X-radiation; adults; animal age; breeds; cardiovascular diseases; clinical examination; dirofilariasis; disease diagnosis; dog diseases; dogs; doxycycline; drugs; echocardiography; electrocardiography; gender; hematologic tests; ivermectin; parasites; respiratory tract diseases; therapeutics; Serbia
Abstract:
... The paper presents clinical diagnostic approaches and therapeutic effects of a specific protocol for the treatment of dogs with cardiovascular dirofilariasis in the Belgrade City (Serbia) territory. The study involved 50 privately owned dogs of different breeds, gender, and age, all showing signs of cardio - respiratory disorders. In addition to a general physical examination, blood tests were don ...
DOI:
10.1515/bvip-2015-0075

9. Experimental infection with epizootic haematopoietic necrosis virus (EHNV) of rainbow trout (Oncorhynchus mykiss Walbaum) and European perch (Perca fluviatilis L.)

Author:
Borzym, Ewa; Maj-Paluch, Joanna
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 473-478
ISSN:
2300-3235
Subject:
Epizootic haematopoietic necrosis virus; Oncorhynchus mykiss; Perca fluviatilis; cell culture; farms; histopathology; models; mortality; necrosis; perch; population; temperature; Poland
Abstract:
... The aim of this study was the determination of the susceptibility of Polish farmed redfin perch (Perca fluviatilis L.) and rainbow trout (Oncorhynchus mykiss Walbaum) to experimental infection with haematopoietic necrosis virus (EHNV). A bath challenge model was tested at two temperature ranges: 13-15°C and 20-22°C. After 7 d, the first clinical signs and mortality were observed in fish kept at th ...
DOI:
10.1515/bvip-2015-0070

10. Genetic diversity of the long terminal repeat of bovine leukaemia virus field isolates

Author:
Pluta, Aneta; Rola-Łuszczak, Marzena; Olech, Monika; Kuźmak, Jacek
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 447-450
ISSN:
2300-3235
Subject:
Bovine leukemia virus; binding sites; cattle; enzootic bovine leukosis; genetic variation; herds; interferons; mutation; nucleotide motifs; terminal repeat sequences; transcription (genetics)
Abstract:
... In this study the sequences of the long terminal repeat (LTR) of field isolates of the bovine leukaemia virus (BLV) were analysed. These isolates came from emerging cases of BLV infection in cattle from herds having BLV-free status. We found several sequence variations within regulatory motifs in the LTRs like GRE, DAS and interferon binding site. These mutations can possibly affect transcriptiona ...
DOI:
10.1515/bvip-2015-0065

11. Innervation of the chinchilla testis, epididymis, and vas deferens

Author:
Sienkiewicz, Waldemar; Szczurkowski, Aleksander; Dudek, Agnieszka; Kaleczyc, Jerzy
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 547-556
ISSN:
2300-3235
Subject:
Chinchilla (Chinchillidae); acetylcholine; anesthesia; animals; dopamine; epididymis; genes; innervation; males; nerve fibers; nerve tissue; neuropeptide Y; seminiferous tubules; substance P; tail; vasoactive intestinal peptide
Abstract:
... The study was performed on six male chinchillas. The animals were anaesthetised with ether and the anaesthesia was deepened with nembuthal injected intraperitoneally. The chinchillas were then transcardially perfused with 0.4 L of 4% buffered paraformaldehyde, and testes, epididymides, and vasa deferentia were collected. The tunica albuginea from one testis from each chinchilla was stained as whol ...
DOI:
10.1515/bvip-2015-0082

12. Magnesium supplements affect selected cadmium toxic actions and uptake of repeated doses of cadmium

Author:
Grosicki, Andrzej; Małagocki, Paweł; Kycko, Anna; Monkiewicz, Jerzy; Korol, Waldemar
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 541-546
ISSN:
2300-3235
Subject:
allometry; body weight; cadmium; diet; drinking water; duodenum; heavy metals; kidneys; liver; magnesium; magnesium chloride; males; rats; testes; toxicity; weight gain
Abstract:
... The importance of magnesium supplements on organ retention of cadmium and allometric parameters after repeated exposure to cadmium chloride were studied in male Wistar rats. Magnesium chloride was given via drinking water (500 mg Mg/L) to rats exposed intragastrically to cadmium chloride (labelled with cadmium 109) at a daily dose corresponding to 25 mg/kg diet for 7, 14, 21, and 28 d. Supplements ...
DOI:
10.1515/bvip-2015-0081

13. Occurrence of tetracyclines in feedingstuffs – results of a two-year study within the official control of feed

Author:
Przeniosło-Siwczyńska, Monika; Patyra, Ewelina; Chyłek-Purchała, Maja; Kozak, Beata; Kwiatek, Krzysztof
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 527-532
ISSN:
2300-3235
Subject:
chromatography; feeds; tetracyclines
Abstract:
... The paper describes a microbiological method for the detection of antibacterial substances in feedingstuffs. The method allowed detection of the main antibiotic groups, including tetracyclines. In 2013-2014, a total of 171 feed samples were analysed to determine antibacterial substances. Among the analysed samples 84 (49.1%) were suspected to contain tetracyclines. Out of the 84 feeds analysed usi ...
DOI:
10.1515/bvip-2015-0079

14. Presence of Escherichia coli O157 and O157:H7 in raw milk and Van herby cheese

Author:
Sancak, Yakup Can; Sancak, Hakan; Isleyici, Ozgur
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 511-514
ISSN:
2300-3235
Subject:
Escherichia coli O157; Food and Drug Administration; cheeses; grocery stores; humans; pathogens; public health; raw milk; risk
Abstract:
... The Shiga toxin-producing Escherichia coli (STEC) strains are currently considered important emerging pathogens threatening public health. Among Shiga toxin-producing Escherichia coli, E. coli O157:H7 strains have emerged as important human pathogens. This study was conducted to determine the presence of Escherichia coli O157 and O157:H7 in raw milk samples and Van herby cheese samples. For this p ...
DOI:
10.1515/bvip-2015-0076

15. Prevalence of bovine herpes virus type 1 in small herds of young beef cattle in south-eastern Poland – a preliminary study

Author:
Wernicki, Andrzej; Urban-Chmiel, Renata; Rola, Jerzy; Socha, Wojciech; Stęgierska, Diana; Dec, Marta; Puchalski, Andrzej
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 467-472
ISSN:
2300-3235
Subject:
nose; Bovine alphaherpesvirus 1; blood serum; antigens; seroprevalence; herds; antibodies; feedlots; farms; beef cattle; polymerase chain reaction; virus transmission; enzyme-linked immunosorbent assay; breeds; trachea (vertebrates); Poland
Abstract:
... The study was performed on nasal swabs, tracheal samples, and sera obtained from young beef heifers aged between 6 and 12 months, from farms in eastern and south-eastern Poland. The samples were evaluated using bovine herpesvirus 1 (BHV-1) ELISA kits (ELISA BHV1 antibody and ELISA BHV1 antigen) and PCR. Among all the animals examined, 37 (32.2%) were positive in the ELISA BHV1 antigen test. The pr ...
DOI:
10.1515/bvip-2015-0069

16. Real-time replication of swine vesicular disease virus (SVDV) in cell culture systems in vitro

Author:
Paprocka, Grażyna; Kęsy, Andrzej
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 457-462
ISSN:
2300-3235
Subject:
correlation; Enterovirus B; cell culture; cytopathogenicity; disease resistance; virus replication; inoculum; tissue culture
Abstract:
... A swine vesicular disease virus (SVDV) replication assay in IB-RS-2, SK-6, and PK-15 cell cultures was performed using the xCELLigence system. The cell status was monitored by impedance measurement, expressed as cell index (CI). Proliferation of particular cells was examined at the beginning of the study. The cells exhibited the ability to form a monolayer, and the CI values increased with the cel ...
DOI:
10.1515/bvip-2015-0067

17. Redox status in the blood of ewes in the perinatal period and during lactation

Author:
Ognik, Katarzyna; Patkowski, Krzysztof; Gruszecki, Tomasz; Kostro, Krzysztof
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 557-562
ISSN:
2300-3235
Subject:
antioxidant activity; bilirubin; blood; catalase; creatinine; ewes; lactation; lambing; lipids; malondialdehyde; oxidative stress; perinatal period; peroxides; pregnancy; spectroscopy; superoxide dismutase; urea; uric acid
Abstract:
... The aim of the study was to determine the effect of the perinatal period on redox status indicators in the blood of ewes before and after lambing and during lactation. The study was performed on 12 ewes of the synthetic SCP line. Blood for testing of redox parameters was collected seven times: before pregnancy, 1.5 months and 24 h before lambing, 2 and 24 h after lambing, and in the fourth and eig ...
DOI:
10.1515/bvip-2015-0083

18. Seroprevalence of Coxiella burnetii in Polish cattle herds

Author:
Jodełko, Agnieszka; Niemczuk, Krzysztof; Szymańska-Czerwińska, Monika
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 479-482
ISSN:
2300-3235
Subject:
Coxiella burnetii; blood sampling; blood serum; cattle; complement fixation tests; herds; seroprevalence; Poland
Abstract:
... The aim of the study was to assess the seroprevalence of Coxiella burnetii in cattle herds in different regions of Poland. A total of 1150 serum samples collected from 443 cattle herds from 14 provinces were tested using complement fixation test. The seroprevalence was different in individual regions of Poland. The average percentage of seropositive herds was 40.41% and these herds were identified ...
DOI:
10.1515/bvip-2015-0071

19. Substitution in position 222 of haemagglutinin of pandemic influenza A (H1N1) viruses isolated from pigs in Poland

Author:
Kowalczyk, Andrzej; Urbaniak, Kinga; Markowska-Daniel, Iwona; Pejsak, Zygmunt
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 451-456
ISSN:
2300-3235
Subject:
Influenza A virus; amino acid sequences; antigenic variation; disease surveillance; genome; hemagglutinins; influenza; mutation; pandemic; receptors; sequence analysis; swine; Poland
Abstract:
... The aim of the study was to monitor genetic diversity and antigenic changes in the genome of influenza A(H1N1)pdm09 viral isolates detected during the post-pandemic period in Poland. Clinical specimens obtained from three suspected cases of influenza were analysed by sequencing. Among the differences identified in amino acids sequences, nine substitutions were located within the antigenic HA1 site ...
DOI:
10.1515/bvip-2015-0066

20. Transcriptional immunoresponse of tissue-specific macrophages in swine after infection with African swine fever virus

Author:
Kowalczyk, Andrzej; Kozak, Edyta; Łyjak, Magdalena; Pejsak, Zygmunt; Niemczuk, Krzysztof
Source:
Bulletin of the Veterinary Institute in Puławy = 2015 v.59 no.4 pp. 441-446
ISSN:
2300-3235
Subject:
immunopathology; transcription factors; phenotype; African swine fever virus; immunosuppression; monocytes; stress response; macrophages; inflammation; pathogens; transcription (genetics); bone marrow; immune response; lungs; chemokines; apoptosis; swine
Abstract:
... Macrophages and cytokines are important in the control of inflammation and regulation of the immune response. However, they can also contribute to immunopathology in the host after viral infection and the regulatory network can be subverted by infectious agents, including viruses, some of which produce cytokine analogues or have mechanisms that inhibit cytokine function. African swine fever virus ...
DOI:
10.1515/bvip-2015-0064