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- Ono, Akiko; Suzuki, Tomohiro; Gotoh, Saki; Kono, Haruka; Matsui, Megumi; Aoki, Daichi; Matsuda, Masaru; Kawagishi, Hirokazu; Ogata, Makoto
- Carbohydrate research 2019 v.475 pp. 27-33
- Asteroidea; alpha-L-fucosidase; ammonium sulfate; bioinformatics; carbohydrates; gel chromatography; gene expression; genes; molecular cloning; molecular weight; pH; pancreas; polyacrylamide gel electrophoresis; sequence analysis
- ... An α-l-fucosidase (Pap-Alf) was purified from the pancreas of a starfish Patiria pectinifera by ammonium sulfate precipitation followed by several column chromatographies. The molecular mass of the purified enzyme was estimated to be 52.6 kDa by SDS-PAGE, although gel filtration analysis of the native enzyme suggests it exists as a homodimer in solution. The purified enzyme showed maximal activity ...