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- Chitwood, Patrick J.; Juszkiewicz, Szymon; Guna, Alina; Shao, Sichen; Hegde, Ramanujan S.
- Cell 2018 v.175 no.6 pp. 1507-1519.e16
- beta-1 adrenergic receptors; biogenesis; endoplasmic reticulum; mammals; signal peptide; topology
- ... Mammals encode ∼5,000 integral membrane proteins that need to be inserted in a defined topology at the endoplasmic reticulum (ER) membrane by mechanisms that are incompletely understood. Here, we found that efficient biogenesis of β1-adrenergic receptor (β1AR) and other G protein-coupled receptors (GPCRs) requires the conserved ER membrane protein complex (EMC). Reconstitution studies of β1AR biog ...
- Zheng, Yuxiang; Lin, Ting-Yu; Lee, Gina; Paddock, Marcia N.; Momb, Jessica; Cheng, Zhe; Li, Qian; Fei, Dennis L.; Stein, Benjamin D.; Ramsamooj, Shivan; Zhang, Guoan; Blenis, John; Cantley, Lewis C.
- Cell 2018 v.175 no.6 pp. 1546-1560.e17
- cell lines; cytosol; formates; gene editing; mammals; methotrexate; mitochondria; neoplasm cells; neoplasms; patients; tetrahydrofolic acid; vitamin B deficiency; vitamin metabolism
- ... Mammalian folate metabolism is comprised of cytosolic and mitochondrial pathways with nearly identical core reactions, yet the functional advantages of such an organization are not well understood. Using genome-editing and biochemical approaches, we find that ablating folate metabolism in the mitochondria of mammalian cell lines results in folate degradation in the cytosol. Mechanistically, we sho ...