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- Mellott, Adam J.; Godsey, Megan E.; Shinogle, Heather E.; Moore, David S.; Forrest, M. Laird; Detamore, Michael S.
- Cellular reprogramming 2014 v.16 no.2 pp. 91-97
- umbilical cord, etc ; apoptosis; cell viability; electroporation; embryonic stem cells; freezing; gene expression; green fluorescent protein; humans; induced pluripotent stem cells; medicine; stromal cells; survival rate; tissue engineering; transfection; Show all 15 Subjects
- ... Differentiating stem cells using gene delivery is a key strategy in tissue engineering and regenerative medicine applications. Nonviral gene delivery bypasses several safety concerns associated with viral gene delivery; however, leading nonviral techniques, such as electroporation, subject cells to high stress and can result in poor cell viabilities. Inhibition of Rho-associated coiled-coil kinase ...
- PubMed Central:
- Marinowic, Daniel Rodrigo; Zanirati, Gabriele; Azevedo, Pamella Nunes; De Souza, Eduardo Vieira; Bruzzo, Fernanda; Silva, Samara Pedroso da; Heuser, Eliete Biasotto; Machado, Denise Cantarelli; Da Costa, Jaderson Costa
- Cellular reprogramming 2018 v.20 no.5 pp. 320-327
- umbilical cord, etc ; adult stem cells; blood; bone marrow; cell cycle; cell viability; coculture; fibroblasts; gene expression; gene expression regulation; genes; humans; messenger RNA; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; therapeutics; toxicity testing; Show all 17 Subjects
- ... The human umbilical cord blood (HUCB) is an excellent source of adult stem cells, having the benefit of being younger than the bone marrow stem cells. The role of stem cells in the lesion repair mechanism is still being studied. We evaluated the capability of HUCB to interfere into the fibroblast dedifferentiation plasticity through cocultivation. Direct and indirect cocultures were maintained for ...