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You searched for: Journal name Chemical Society reviews Remove constraint Journal name: Chemical Society reviews Publication year rev 7986-2014 Remove constraint Publication year rev: 7986-2014 Source 2014 v.43 no.4 Remove constraint Source: 2014 v.43 no.4
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1. Alternating-laser excitation: single-molecule FRET and beyond

Author:
Johannes Hohlbein; Timothy D. Craggs; Thorben Cordes
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1156-1171
ISSN:
1460-4744
Subject:
bioassays; energy transfer; fluorescent dyes; wills
Abstract:
... The alternating-laser excitation (ALEX) scheme continues to expand the possibilities of fluorescence-based assays to study biological entities and interactions. Especially the combination of ALEX and single-molecule Förster Resonance Energy Transfer (smFRET) has been very successful as ALEX enables the sorting of fluorescently labelled species based on the number and type of fluorophores present. ...
DOI:
10.1039/c3cs60233h

2. Breaking the concentration limit of optical single-molecule detection

Author:
Phil Holzmeister; Guillermo P. Acuna; Dina Grohmann; Philip Tinnefeld
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1014-1028
ISSN:
1460-4744
Subject:
fluorescence; motivation; signal-to-noise ratio
Abstract:
... Over the last decade, single-molecule detection has been successfully utilized in the life sciences and materials science. Yet, single-molecule measurements only yield meaningful results when working in a suitable, narrow concentration range. On the one hand, diffraction limits the minimal size of the observation volume in optical single-molecule measurements and consequently a sample must be adeq ...
DOI:
10.1039/c3cs60207a

3. Deconstructing the photon stream from single nanocrystals: from binning to correlation

Author:
Jian Cui; Andrew P. Beyler; Thomas S. Bischof; Mark W. B. Wilson; Moungi G. Bawendi
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1287-1310
ISSN:
1460-4744
Subject:
fluorescence emission spectroscopy; nanocrystals; photons; quantum dots
Abstract:
... Prior to the advent of single-molecule fluorescence spectroscopy, many of the fundamental optical properties of colloidal semiconductor nanocrystal quantum dots were obscured by ensemble averaging over their inherent inhomogeneities. Single quantum dot spectroscopy has become a leading technique for the unambiguous determination of the governing excitonic physics of these quantum-confined systems. ...
DOI:
10.1039/c3cs60330j

4. Enhanced single-molecule spectroscopy in highly confined optical fields: from λ/2-Fabry–Pérot resonators to plasmonic nano-antennas

Author:
Andreas M. Kern; Dai Zhang; Marc Brecht; Alexey I. Chizhik; Antonio Virgilio Failla; Frank Wackenhut; Alfred J. Meixner
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1263-1286
ISSN:
1460-4744
Subject:
detection limit; dyes; energy transfer; fluorescence; models
Abstract:
... While single-molecule fluorescence from emitters with high quantum efficiencies such as organic dye molecules can easily be detected by modern apparatus, many less efficient emission processes such as Raman scattering and metal luminescence require dramatic enhancement to exceed the single-particle detection limit. This enhancement can be achieved using resonant optical systems such as plasmonic p ...
DOI:
10.1039/c3cs60357a

5. Maximizing information content of single-molecule FRET experiments: multi-color FRET and FRET combined with force or torque

Author:
Sungchul Hohng; Sanghwa Lee; Jinwoo Lee; Myung Hyun Jo
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1007-1013
ISSN:
1460-4744
Subject:
energy transfer; fluorescence; torque
Abstract:
... Since its first demonstration about twenty years ago, single-molecule fluorescence resonance energy transfer (FRET) has undergone remarkable technical advances. In this tutorial review, we will discuss two technical advances that increase the information content of the single-molecule FRET measurements: single-molecule multi-color FRET and single-molecule FRET combined with force or torque. Our ex ...
DOI:
10.1039/c3cs60184f

6. Shedding light on protein folding landscapes by single-molecule fluorescence

Author:
Priya R. Banerjee; Ashok A. Deniz
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1172-1188
ISSN:
1460-4744
Subject:
Gibbs free energy; basins; fluorescence
Abstract:
... Single-molecule (SM) fluorescence methods have been increasingly instrumental in our current understanding of a number of key aspects of protein folding and aggregation landscapes over the past decade. With the advantage of a model free approach and the power of probing multiple subpopulations and stochastic dynamics directly in a heterogeneous structural ensemble, SM methods have emerged as a pri ...
DOI:
10.1039/c3cs60311c
PubMed:
24336839
PubMed Central:
PMC3958939

7. Single molecule methods for the study of catalysis: from enzymes to heterogeneous catalysts

Author:
Kris P. F. Janssen; Gert De Cremer; Robert K. Neely; Alexey V. Kubarev; Jordi Van Loon; Johan A. Martens; Dirk E. De Vos; Maarten B. J. Roeffaers; Johan Hofkens
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 990-1006
ISSN:
1460-4744
Subject:
catalytic activity; fluorescence emission spectroscopy; fluorescence microscopy
Abstract:
... Structural and temporal inhomogeneities can have a marked influence on the performance of inorganic and biocatalytic systems alike. While these subtle variations are hardly ever accessible through bulk or ensemble averaged activity screening, insights into the molecular mechanisms underlying these diverse phenomena are absolutely critical for the development of optimized or novel catalytic systems ...
DOI:
10.1039/c3cs60245a

8. Single molecule rotational probing of supercooled liquids

Author:
Keewook Paeng; Laura J. Kaufman
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 977-989
ISSN:
1460-4744
Subject:
environment; fields; fluorescence microscopy; hands; measurement
Abstract:
... Much of the interesting behavior that has been observed in supercooled liquids appears to be related to dynamic heterogeneity, the presence of distinct dynamic environments – with no apparent underlying structural basis – in these systems. To most directly interrogate these environments, proposed to span regions just a few nanometers across, molecular length scale probes are required. Single molec ...
DOI:
10.1039/c3cs60186b

9. Single-molecule photophysics, from cryogenic to ambient conditions

Author:
Bolesław Kozankiewicz; Michel Orrit
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1029-1043
ISSN:
1460-4744
Subject:
couplings; fields; fluorescence; materials; temperature; vibration
Abstract:
... We review recent progress in characterizing and understanding the photophysics of single molecules in condensed matter, mostly at cryogenic temperatures. We discuss the central role of the triplet state in limiting the number of useful host–guest systems, notably a new channel, intermolecular intersystem crossing. Another important limitation to the use of single molecules is their photo-reactivit ...
DOI:
10.1039/c3cs60165j

10. Spatiotemporal catalytic dynamics within single nanocatalysts revealed by single-molecule microscopy

Author:
Peng Chen; Xiaochun Zhou; Nesha May Andoy; Kyu-Sung Han; Eric Choudhary; Ningmu Zou; Guanqun Chen; Hao Shen
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1107-1117
ISSN:
1460-4744
Subject:
catalytic activity; fluorescence; microscopy; nanocatalysts; nanocrystals; nanorods
Abstract:
... This review discusses the latest advances in using single-molecule microscopy of fluorogenic reactions to examine and understand the spatiotemporal catalytic behaviors of single metal nanoparticles of various shapes including pseudospheres, nanorods, and nanoplates. Real-time single-turnover kinetics reveal size-, catalysis-, and metal-dependent temporal activity fluctuations of single pseudospher ...
DOI:
10.1039/c3cs60215j

11. Spectroscopy of single nanocrystals

Author:
Mark J. Fernée; Philippe Tamarat; Brahim Lounis
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1311-1337
ISSN:
1460-4744
Subject:
cadmium; nanocrystals; photoluminescence; selenides; semiconductors; spectroscopy
Abstract:
... As colloidal semiconductor nanocrystals are developed for a wider range of diverse applications, it becomes more important to gain a deeper understanding of their properties in order to direct synthetic efforts. While most synthetic developments are guided by changes in ensemble properties, certain applications such as those in nano-electronics and nano-photonics rely on properties of nanocrystals ...
DOI:
10.1039/c3cs60209e

12. Tip-enhanced near-field optical microscopy

Author:
Nina Mauser; Achim Hartschuh
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1248-1262
ISSN:
1460-4744
Subject:
detection limit; electric current; electrochemiluminescence; fluorescence; light microscopy
Abstract:
... Tip-enhanced near-field optical microscopy (TENOM) is a scanning probe technique capable of providing a broad range of spectroscopic information on single objects and structured surfaces at nanometer spatial resolution and with highest detection sensitivity. In this review, we first illustrate the physical principle of TENOM that utilizes the antenna function of a sharp probe to efficiently couple ...
DOI:
10.1039/c3cs60258c
PubMed:
24100541
PubMed Central:
PMC3907270

13. Visualizing cellular machines with colocalization single molecule microscopy

Author:
Joshua D. Larson; Margaret L. Rodgers; Aaron A. Hoskins
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1189-1200
ISSN:
1460-4744
Subject:
RNA; microscopy; spectroscopy
Abstract:
... Many of the cell's macromolecular machines contain multiple components that transiently associate with one another. This compositional and dynamic complexity presents a challenge for understanding how these machines are constructed and function. Colocalization single molecule spectroscopy enables simultaneous observation of individual components of these machines in real-time and grants a unique w ...
DOI:
10.1039/c3cs60208g
PubMed:
23970346
PubMed Central:
PMC3946777

14. DNA replication at the single-molecule level

Author:
S. A. Stratmann; A. M. van Oijen
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1201-1220
ISSN:
1460-4744
Subject:
DNA; DNA damage; DNA replication; RNA; metabolites; oligonucleotides; physiological transport; protein synthesis
Abstract:
... A cell can be thought of as a highly sophisticated micro factory: in a pool of billions of molecules – metabolites, structural proteins, enzymes, oligonucleotides – multi-subunit complexes assemble to perform a large number of basic cellular tasks, such as DNA replication, RNA/protein synthesis or intracellular transport. By purifying single components and using them to reconstitute molecular proc ...
DOI:
10.1039/c3cs60391a

15. Fluorescent proteins for live-cell imaging with super-resolution

Author:
Karin Nienhaus; G. Ulrich Nienhaus
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1088-1106
ISSN:
1460-4744
Subject:
fluorescence; irradiation; isomerization; lighting; microscopy; protonation; wavelengths
Abstract:
... Fluorescent proteins (FPs) from the GFP family have become indispensable as marker tools for imaging live cells, tissues and entire organisms. A wide variety of these proteins have been isolated from natural sources and engineered to optimize their properties as genetically encoded markers. Here we review recent developments in this field. A special focus is placed on photoactivatable FPs, for whi ...
DOI:
10.1039/c3cs60171d

16. Förster resonance energy transfer and kinesin motor proteins

Author:
Bram Prevo; Erwin J. G. Peterman
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1144-1155
ISSN:
1460-4744
Subject:
active transport; energy transfer; fluorescent dyes; kinesin; microscopy; microtubules; molecular motor proteins; spectroscopy
Abstract:
... Förster Resonance Energy Transfer (FRET) is the phenomenon of non-radiative transfer of electronic excitations from a donor fluorophore to an acceptor, mediated by electronic dipole–dipole coupling. The transfer rate and, as a consequence, efficiency depend non-linearly on the distance between the donor and the acceptor. FRET efficiency can thus be used as an effective and accurate reporter of dis ...
DOI:
10.1039/c3cs60292c

17. How to switch a fluorophore: from undesired blinking to controlled photoswitching

Author:
Sebastian van de Linde; Markus Sauer
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1076-1087
ISSN:
1460-4744
Subject:
fluorescence; fluorescent dyes; microscopy
Abstract:
... Molecular optical photoswitches based on fluorescent proteins and organic dyes are fundamental for super-resolution fluorescence imaging and tracking methods. Precise control of switching, bio-labeling compatibility, and high brightness make photoswitches broadly applicable. This review emphasizes the design and development of photoswitches and the requirements they need to fulfill for their succe ...
DOI:
10.1039/c3cs60195a

18. Photophysical processes in single molecule organic fluorescent probes

Author:
Elana M. S. Stennett; Monika A. Ciuba; Marcia Levitus
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1057-1075
ISSN:
1460-4744
Subject:
fluorescence; fluorescence microscopy; fluorescent dyes; photobleaching; spectroscopy
Abstract:
... The use of organic fluorescent probes in biochemical and biophysical applications of single molecule spectroscopy and fluorescence microscopy techniques continues to increase. As single molecule measurements become more quantitative and new developments in super-resolution imaging allow researchers to image biological materials with unprecedented resolution, it is becoming increasingly important t ...
DOI:
10.1039/c3cs60211g

19. Protein induced fluorescence enhancement (PIFE) for probing protein–nucleic acid interactions

Author:
Helen Hwang; Sua Myong
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1221-1229
ISSN:
1460-4744
Subject:
DNA; RNA; dyes; fluorescence
Abstract:
... Single molecule studies of protein–nucleic acid interactions shed light on molecular mechanisms and kinetics involved in protein binding, translocation, and unwinding of DNA and RNA substrates. In this review, we provide an overview of a single molecule fluorescence method, termed “protein induced fluorescence enhancement” (PIFE). Unlike FRET where two dyes are required, PIFE employs a single dye ...
DOI:
10.1039/c3cs60201j
PubMed:
24056732
PubMed Central:
PMC4142350

20. Sizing up single-molecule enzymatic conformational dynamics

Author:
H. Peter Lu
Source:
Chemical Society reviews 2014 v.43 no.4 pp. 1118-1143
ISSN:
1460-4744
Subject:
enzymatic reactions; enzymes; enzymology; spectroscopy; theoretical models
Abstract:
... Enzymatic reactions and related protein conformational dynamics are complex and inhomogeneous, playing crucial roles in biological functions. The relationship between protein conformational dynamics and enzymatic reactions has been a fundamental focus in modern enzymology. It is extremely difficult to characterize and analyze such complex dynamics in an ensemble-averaged measurement, especially wh ...
DOI:
10.1039/c3cs60191a