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1. Efficient gene targeting in non-homologous end-joining-deficient Lipomyces starkeyi strains

Author:
Oguro, Yoshifumi; Yamazaki, Harutake; Ara, Satoshi; Shida, Yosuke; Ogasawara, Wataru; Takagi, Masamichi; Takaku, Hiroaki
Source:
Current genetics 2017 v.63 no.4 pp. 751-763
ISSN:
0172-8083
Subject:
Lipomyces starkeyi; animals; biodiesel; biosynthesis; cost effectiveness; drug resistance; feedstocks; gene targeting; genes; genetic engineering; homologous recombination; industry; lipids; loci; metabolic engineering; mutants; plants (botany); yeasts
Abstract:
... Microbial lipids are sustainable feedstock for the production of oleochemicals and biodiesel. Oleaginous yeasts have recently been proposed as alternative lipid producers to plants and animals to promote sustainability in the chemical and fuel industries. The oleaginous yeast Lipomyces starkeyi has great industrial potential as an excellent lipid producer. However, improvement of its lipid product ...
DOI:
10.1007/s00294-017-0679-6

2. FvSNF1, the sucrose non-fermenting protein kinase gene of Fusarium virguliforme, is required for cell-wall-degrading enzymes expression and sudden death syndrome development in soybean

Author:
Islam, Kazi T.; Bond, Jason P.; Fakhoury, Ahmad M.
Source:
Current genetics 2017 v.63 no.4 pp. 723-738
ISSN:
0172-8083
Subject:
Fusarium virguliforme; agglutinins; arabinose; carbon; death; fructose; galactose; genes; glucose; greenhouses; maltose; microbial biomass; microscopy; mutants; oxidation; pectins; phloem; plant pathogenic fungi; protein kinases; roots; soybeans; sucrose; virulence; wheat germ; xylem vessels; xylose; yeasts
Abstract:
... Fusarium virguliforme is a soil-borne pathogenic fungus that causes sudden death syndrome (SDS) in soybean. Its pathogenicity is believed to require the activity of cell-wall-degrading enzymes (CWDEs). The sucrose non-fermenting protein kinase 1 gene (SNF1) is a key component of the glucose de-repression pathway in yeast, and a regulator of gene expression for CWDEs in some plant pathogenic fungi. ...
DOI:
10.1007/s00294-017-0676-9

3. Putative orotate transporter of Cryptococcus neoformans, Oat1, is a member of the NCS1/PRT transporter super family and its loss causes attenuation of virulence

Author:
Toh-e, Akio; Ohkusu, Misako; Shimizu, Kiminori; Takahashi-Nakaguchi, Azusa; Kawamoto, Susumu; Ishiwada, Naruhiko; Watanabe, Akira; Kamei, Katsuhiko
Source:
Current genetics 2017 v.63 no.4 pp. 697-707
ISSN:
0172-8083
Subject:
Cryptococcus neoformans; Saccharomyces cerevisiae; allantoin; fungi; genes; genetic background; metabolites; mutants; mutation; nitrogen; orotic acid; phenotype; proline; symporters; transcription factors; uracil; uridine; virulence
Abstract:
... It is well known that 5-fluoroorotic acid (5-FOA)-resistant mutants isolated from wild-type Cryptococcus neoformans are exclusively either ura3 or ura5 mutants. Unexpectedly, many of the 5-FOA-resistant mutants isolated in our selective regime were Ura⁺. We identified CNM00460 as the gene responsible for these mutations. Cnm00460 belongs to the nucleobase cation symporter 1/purine-related transpor ...
DOI:
10.1007/s00294-016-0672-5

4. The functions of the multi-tasking Pfh1Pif1 helicase

Author:
Sabouri, Nasim
Source:
Current genetics 2017 v.63 no.4 pp. 621-626
ISSN:
0172-8083
Subject:
Schizosaccharomyces pombe; breast neoplasms; eukaryotic cells; genes; humans; metabolism; mitochondrial genome; molecular motor proteins; nuclear genome; nucleic acids
Abstract:
... Approximately, 1% of the genes in eukaryotic genomes encode for helicases, which make the number of helicases expressed in the cell considerably high. Helicases are motor proteins that participate in many central aspects of the nuclear and mitochondrial genomes, and based on their helicase motif conservation, they are divided into different helicase families. The Pif1 family of helicases is an evo ...
DOI:
10.1007/s00294-016-0675-2
PubMed:
28054200
PubMed Central:
PMC5504263

5. The loading of condensin in the context of chromatin

Author:
Robellet, Xavier; Vanoosthuyse, Vincent; Bernard, Pascal
Source:
Current genetics 2017 v.63 no.4 pp. 577-589
ISSN:
0172-8083
Subject:
DNA; genes; models; nucleosomes; transcription (genetics); transcription factors
Abstract:
... The packaging of DNA into chromosomes is a ubiquitous process that enables living organisms to structure and transmit their genome accurately through cell divisions. In the three kingdoms of life, the architecture and dynamics of chromosomes rely upon ring-shaped SMC (Structural Maintenance of Chromosomes) condensin complexes. To understand how condensin rings organize chromosomes, it is essential ...
DOI:
10.1007/s00294-016-0669-0

6. Transcriptomic analysis displays the effect of (-)-roemerine on the motility and nutrient uptake in Escherichia coli

Author:
Ayyildiz, Dilara; Arga, Kazim Yalcin; Avci, Fatma Gizem; Altinisik, Fatma Ece; Gurer, Caglayan; Gulsoy Toplan, Gizem; Kazan, Dilek; Wozny, Katharina; Brügger, Britta; Mertoglu, Bulent; Sariyar Akbulut, Berna
Source:
Current genetics 2017 v.63 no.4 pp. 709-722
ISSN:
0172-8083
Subject:
Escherichia coli; alkaloids; amino acids; antibacterial properties; biofilm; cell movement; defense mechanisms; energy; energy metabolism; genes; lipids; mechanism of action; microarray technology; nutrient uptake; outer membrane proteins; quantitative polymerase chain reaction; transcription (genetics); transcriptomics
Abstract:
... Among the different families of plant alkaloids, (-)-roemerine, an aporphine type, was recently shown to possess significant antibacterial activity in Escherichia coli. Based on the increasing demand for antibacterials with novel mechanisms of action, the present work investigates the potential of the plant-derived alkaloid (-)-roemerine as an antibacterial in E. coli cells using microarray techno ...
DOI:
10.1007/s00294-016-0673-4

7. WD40-repeat protein MoCreC is essential for carbon repression and is involved in conidiation, growth and pathogenicity of Magnaporthe oryzae

Author:
Matar, Khalid Abdelkarim Omer; Chen, Xiaofeng; Chen, Dongjie; Anjago, Wilfred Mabeche; Norvienyeku, Justice; Lin, Yahong; Chen, Meilian; Wang, Zonghua; Ebbole, Daniel J.; Lu, Guo-dong
Source:
Current genetics 2017 v.63 no.4 pp. 685-696
ISSN:
0172-8083
Subject:
2-deoxyglucose; Aspergillus nidulans; Magnaporthe oryzae; allyl alcohol; appressoria; beta-glucosidase; carbon; cell walls; conidia; conidiation; feruloyl esterase; gene expression; gene expression regulation; genes; glucose; host plants; hyphae; leaves; microorganisms; mutants; pathogenesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; vegetative growth; virulence
Abstract:
... Carbon catabolite repression (CCR) is a common regulatory mechanism used by microorganisms to prioritize use of a preferred carbon source (usually glucose). The CreC WD40-repeat protein is a major component of the CCR pathway in Aspergillus nidulans. To clarify the function of the CreC ortholog from Magnaporthe oryzae in regulating gene expression important for pathogenesis, MoCreC was identified ...
DOI:
10.1007/s00294-016-0668-1