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1. Efficient gene targeting in non-homologous end-joining-deficient Lipomyces starkeyi strains

Author:
Oguro, Yoshifumi; Yamazaki, Harutake; Ara, Satoshi; Shida, Yosuke; Ogasawara, Wataru; Takagi, Masamichi; Takaku, Hiroaki
Source:
Current genetics 2017 v.63 no.4 pp. 751-763
ISSN:
0172-8083
Subject:
Lipomyces starkeyi; animals; biodiesel; biosynthesis; cost effectiveness; drug resistance; feedstocks; gene targeting; genes; genetic engineering; homologous recombination; industry; lipids; loci; metabolic engineering; mutants; plants (botany); yeasts
Abstract:
... Microbial lipids are sustainable feedstock for the production of oleochemicals and biodiesel. Oleaginous yeasts have recently been proposed as alternative lipid producers to plants and animals to promote sustainability in the chemical and fuel industries. The oleaginous yeast Lipomyces starkeyi has great industrial potential as an excellent lipid producer. However, improvement of its lipid product ...
DOI:
10.1007/s00294-017-0679-6

2. FvSNF1, the sucrose non-fermenting protein kinase gene of Fusarium virguliforme, is required for cell-wall-degrading enzymes expression and sudden death syndrome development in soybean

Author:
Islam, Kazi T.; Bond, Jason P.; Fakhoury, Ahmad M.
Source:
Current genetics 2017 v.63 no.4 pp. 723-738
ISSN:
0172-8083
Subject:
Fusarium virguliforme; agglutinins; arabinose; carbon; death; fructose; galactose; genes; glucose; greenhouses; maltose; microbial biomass; microscopy; mutants; oxidation; pectins; phloem; plant pathogenic fungi; protein kinases; roots; soybeans; sucrose; virulence; wheat germ; xylem vessels; xylose; yeasts
Abstract:
... Fusarium virguliforme is a soil-borne pathogenic fungus that causes sudden death syndrome (SDS) in soybean. Its pathogenicity is believed to require the activity of cell-wall-degrading enzymes (CWDEs). The sucrose non-fermenting protein kinase 1 gene (SNF1) is a key component of the glucose de-repression pathway in yeast, and a regulator of gene expression for CWDEs in some plant pathogenic fungi. ...
DOI:
10.1007/s00294-017-0676-9

3. Genetic evidence for functional interaction of Smc5/6 complex and Top1 with spatial frequency of replication origins required for maintenance of chromosome stability

Author:
Rai, Ragini; Laloraya, Shikha
Source:
Current genetics 2017 v.63 no.4 pp. 765-776
ISSN:
0172-8083
Subject:
chromosome elimination; mutants; mutation; replication origin; yeast artificial chromosomes
Abstract:
... Replication of linear chromosomes is facilitated by firing of multiple replication origins that ensures timely duplication of the entire chromosome. The Smc5/6 complex is thought to play an important role in replication by its involvement in the restart of collapsed replication forks. Here, we present genetic evidence for functional interaction between replication origin distribution and two subun ...
DOI:
10.1007/s00294-017-0680-0

4. Interplay between Top1 and Mms21/Nse2 mediated sumoylation in stable maintenance of long chromosomes

Author:
Mahendrawada, Lakshmi; Rai, Ragini; Kothiwal, Deepash; Laloraya, Shikha
Source:
Current genetics 2017 v.63 no.4 pp. 627-645
ISSN:
0172-8083
Subject:
DNA damage; mutants; mutation; phenotype; viability; yeast artificial chromosomes; yeasts
Abstract:
... Genetic information in cells is encrypted in DNA molecules forming chromosomes of varying sizes. Accurate replication and partitioning of chromosomes in the crowded cellular milieu is a complex process involving duplication, folding and movement. Longer chromosomes may be more susceptible to mis-segregation or DNA damage and there may exist specialized physiological mechanisms preventing this. Her ...
DOI:
10.1007/s00294-016-0665-4

5. Putative orotate transporter of Cryptococcus neoformans, Oat1, is a member of the NCS1/PRT transporter super family and its loss causes attenuation of virulence

Author:
Toh-e, Akio; Ohkusu, Misako; Shimizu, Kiminori; Takahashi-Nakaguchi, Azusa; Kawamoto, Susumu; Ishiwada, Naruhiko; Watanabe, Akira; Kamei, Katsuhiko
Source:
Current genetics 2017 v.63 no.4 pp. 697-707
ISSN:
0172-8083
Subject:
Cryptococcus neoformans; Saccharomyces cerevisiae; allantoin; fungi; genes; genetic background; metabolites; mutants; mutation; nitrogen; orotic acid; phenotype; proline; symporters; transcription factors; uracil; uridine; virulence
Abstract:
... It is well known that 5-fluoroorotic acid (5-FOA)-resistant mutants isolated from wild-type Cryptococcus neoformans are exclusively either ura3 or ura5 mutants. Unexpectedly, many of the 5-FOA-resistant mutants isolated in our selective regime were Ura⁺. We identified CNM00460 as the gene responsible for these mutations. Cnm00460 belongs to the nucleobase cation symporter 1/purine-related transpor ...
DOI:
10.1007/s00294-016-0672-5

6. Transcription factor Afmac1 controls copper import machinery in Aspergillus fumigatus

Author:
Kusuya, Yoko; Hagiwara, Daisuke; Sakai, Kanae; Yaguchi, Takashi; Gonoi, Tohru; Takahashi, Hiroki
Source:
Current genetics 2017 v.63 no.4 pp. 777-789
ISSN:
0172-8083
Subject:
Aspergillus fumigatus; biosynthesis; conidia; conidiation; copper; homeostasis; melanin; mutants; toxicity; transcription factors; transporters; yeasts
Abstract:
... Copper (Cu) is an essential metal for all living organisms, although it is toxic in excess. Filamentous fungus must acquire copper from its environment for growth. Despite its essentiality for growth, the mechanisms that maintain copper homeostasis are not fully understood in filamentous fungus. To gain insights into copper homeostasis, we investigated the roles of a copper transcription factor Af ...
DOI:
10.1007/s00294-017-0681-z

7. WD40-repeat protein MoCreC is essential for carbon repression and is involved in conidiation, growth and pathogenicity of Magnaporthe oryzae

Author:
Matar, Khalid Abdelkarim Omer; Chen, Xiaofeng; Chen, Dongjie; Anjago, Wilfred Mabeche; Norvienyeku, Justice; Lin, Yahong; Chen, Meilian; Wang, Zonghua; Ebbole, Daniel J.; Lu, Guo-dong
Source:
Current genetics 2017 v.63 no.4 pp. 685-696
ISSN:
0172-8083
Subject:
2-deoxyglucose; Aspergillus nidulans; Magnaporthe oryzae; allyl alcohol; appressoria; beta-glucosidase; carbon; cell walls; conidia; conidiation; feruloyl esterase; gene expression; gene expression regulation; genes; glucose; host plants; hyphae; leaves; microorganisms; mutants; pathogenesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; vegetative growth; virulence
Abstract:
... Carbon catabolite repression (CCR) is a common regulatory mechanism used by microorganisms to prioritize use of a preferred carbon source (usually glucose). The CreC WD40-repeat protein is a major component of the CCR pathway in Aspergillus nidulans. To clarify the function of the CreC ortholog from Magnaporthe oryzae in regulating gene expression important for pathogenesis, MoCreC was identified ...
DOI:
10.1007/s00294-016-0668-1