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You searched for: Journal name Developmental and comparative immunology Remove constraint Journal name: Developmental and comparative immunology
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1. A Dicer2 from Scylla paramamosain activates JAK/STAT signaling pathway to restrain mud crab reovirus

Author:
Hengwei Deng; Danrong Xian; Taixin Lian; Mingyu He; Jingjing Li; Xiaopeng Xu; Zhixun Guo; Jianguo He; Shaoping Weng
Source:
Developmental and comparative immunology 2022 v.127 pp. 104267
ISSN:
0145-305X
Subject:
Reoviridae; Scylla paramamosain; Staphylococcus aureus; Vibrio parahaemolyticus; crabs; cytoplasm; genes; hemocytes; immunology; intestines; mortality; muscles; viral load
Abstract:
... A Dicer2 gene from Scylla paramamosain, named SpDicer2, was cloned and characterized. The full length of SpDicer2 mRNA contains a 121 bp 5'untranslated region (UTR), an open reading frame (ORF) of 4518 bp and a 3′ UTR of 850 bp. The SpDicer2 protein contains seven characteristic Dicer domains and showed 34%–65% identity and 54%–79% similarity to other Dicer protein domains, respectively. The mRNA ...
DOI:
10.1016/j.dci.2021.104267

2. A cypovirus encoded microRNA negatively regulates the NF-κB pathway to enhance viral multiplication in Silkworm, Bombyx mori

Author:
Ze Zhao; Su Lin; Wanming Wu; Zhendong Zhang; Ping Wu; Manman Shen; Heying Qian; Xijie Guo
Source:
Developmental and comparative immunology 2022 v.131 pp. 104382
ISSN:
0145-305X
Subject:
Bombyx mori; Cypovirus; RNA interference; biogenesis; double-stranded RNA; eukaryotic cells; gene expression; genes; immunology; microRNA; quantitative polymerase chain reaction; silkworms; viral genome; virus replication
Abstract:
... MicroRNAs (miRNAs) are small non-coding RNAs that function as novel gene expression regulators at the post-transcriptional level. Not with standing that the biogenesis and function of miRNAs are well-understood in eukaryotes, little is known about RNA virus-encoded miRNAs. Bombyx mori cypovirus (BmCPV) is a double-stranded RNA virus with a segmented genome that causes cytoplasmic polyhedrosis dise ...
DOI:
10.1016/j.dci.2022.104382

3. A flow cytometry based approach to identify distinct coelomocyte subsets of the purple sea urchin, Strongylocentrotus purpuratus

Author:
Megan A. Barela Hudgell; Leon Grayfer; L. Courtney Smith
Source:
Developmental and comparative immunology 2022 v.130 pp. 104352
ISSN:
0145-305X
Subject:
Strongylocentrotus purpuratus; coelomocytes; flow cytometry; immune response; microscopy
Abstract:
... The sea urchin, Strongylocentrotus purpuratus, possesses at least seven distinguishable cell populations in the coelomic fluid, which vary in morphology, size, and function. Of these, the large phagocytes, small phagocytes, and red spherule cells are thought to be key to the echinoid immune response. Because there are currently no effective and rapid means of evaluating sea urchin coelomocytes, we ...
DOI:
10.1016/j.dci.2022.104352

4. A new calnexin modulates antibacterial immune response in obscure puffer Takifugu obscurus

Author:
Ying Huang; Rui-Xia Wang; Fu-Hui Jiang; Xiao-Tong Xu; Yan Shi; Zhe Zhao
Source:
Developmental and comparative immunology 2022 v.127 pp. 104288
ISSN:
0145-305X
Subject:
Aeromonas hydrophila; Edwardsiella tarda; Takifugu flavidus; Takifugu obscurus; Takifugu rubripes; Vibrio harveyi; amino acids; calcium; calnexin; calreticulin; endoplasmic reticulum; fish; immune response; intestines; kidneys; lipopolysaccharides; liver; molecular weight; peptidoglycans; phylogeny; polypeptides; sequence analysis
Abstract:
... Calnexin (Cnx) is a membrane-bound lectin chaperone of the endoplasmic reticulum. In this study, a novel Cnx homologue from the obscure puffer Takifugu obscurus was characterized, tentatively named ToCnx. The cDNA of ToCnx was 1803 bp, and it contained an open reading frame encoding a polypeptide of 600 amino acid residues with a calculated molecular weight of 67.5 kDa. Multiple alignment of the d ...
DOI:
10.1016/j.dci.2021.104288

5. A newly identified NLR-like gene participates in bacteria and virus infection possibly through regulating hemocytes apoptosis in shrimp

Author:
Xuechun Li; Shihao Li; Mingzhe Sun; Yang Yu; Xiaojun Zhang; Jianhai Xiang; Fuhua Li
Source:
Developmental and comparative immunology 2022 v.132 pp. 104395
ISSN:
0145-305X
Subject:
Litopenaeus vannamei; Vibrio parahaemolyticus; White spot syndrome virus; apoptosis; bacterial infections; genes; hemocytes; innate immunity; mortality; oligomerization; shrimp; viruses
Abstract:
... The nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) play important roles in innate immunity. Previously, we identified an NLR-like gene, LvNLRPL1, and found that it participated in Vibrio infection and regulated hemocytes apoptosis in the Pacific whiteleg shrimp Litopenaeus vannamei. However, it is still unclear whether other NLR-like genes exist in shrimp and how they functi ...
DOI:
10.1016/j.dci.2022.104395

6. A novel C-type lectin LvCTL 4.2 has antibacterial activity but facilitates WSSV infection in shrimp (L. vannamei)

Author:
Yu-Hsun Huang; Ramya Kumar; Chun-Hung Liu; Shih-Shun Lin; Han-Ching Wang
Source:
Developmental and comparative immunology 2022 v.126 pp. 104239
ISSN:
0145-305X
Subject:
Litopenaeus vannamei; Vibrio parahaemolyticus; White spot syndrome virus; antibacterial properties; growth retardation; hepatopancreas; innate immunity; lectins; necrosis; pathogenesis; pathogens; shrimp; signal peptide; stomach; virion; virus replication
Abstract:
... Glycan-binding protein C-type lectin (CTL), one of the pattern recognition receptors (PRRs), binds to carbohydrates on the surface of pathogens and elicits antimicrobial responses in shrimp innate immunity. The objective was to identify and characterize a novel C-type lectin LvCTL 4.2 in Litopenaeus vannamei. The LvCTL 4.2 protein consisted of a signal peptide at the N terminal and a carbohydrate- ...
DOI:
10.1016/j.dci.2021.104239

7. A novel ML protein functions as a pattern recognition protein in antibacterial responses in Eriocheir sinensis

Author:
Yu Song; Kaimin Zhou; Xingyu Nan; Yukai Qin; Ke Zhao; Weiwei Li; Qun Wang
Source:
Developmental and comparative immunology 2022 v.127 pp. 104310
ISSN:
0145-305X
Subject:
Eriocheir sinensis; Staphylococcus aureus; Vibrio parahaemolyticus; antibacterial properties; bacterial infections; crabs; hemocytes; lipid metabolism; lipopolysaccharides; peptidoglycans; phagocytosis; signal transduction
Abstract:
... The myeloid differentiation factor 2 (MD-2)-related lipid recognition (ML) domain is present in MD-2, MD-1, GM2-activator protein (GM2A) and Niemann-Pick disease type C2 (NPC2). ML proteins function in antibacterial signal transduction and lipid metabolism in vertebrates, but the mechanism in invertebrates is unknown. In this study, we found that ML proteins were involved in bacterial resistance i ...
DOI:
10.1016/j.dci.2021.104310

8. A novel molluscan TLR molecule engaged in inflammatory response through MyD88 adapter recruitment

Author:
Pengzhi Qi; Yashu Wu; Zhongqi Gu; Hongfei Li; Jiji Li; Baoying Guo; Zhi Liao; Xiaojun Yan
Source:
Developmental and comparative immunology 2022 v.131 pp. 104373
ISSN:
0145-305X
Subject:
Mytilus coruscus; adaptive immunity; cytokines; evolution; gene expression; genes; hemocytes; inflammation; mammals; mussels; phosphorylation; transcription (genetics)
Abstract:
... Toll-like receptors (TLRs) mediated signaling plays a vital role in activating innate and adaptive immunity. Although TLR mediated signaling has been comprehensively investigated in mammalian species, the mechanisms underlying TLR signaling in molluscs remain obscure. In the present study, a novel TLR isoform namely McTLR-like1 was identified in the thick shell mussel Mytilus coruscus. McTLR-like1 ...
DOI:
10.1016/j.dci.2022.104373

9. A specific primed immune response in red palm weevil, Rhynchophorus ferrugineus, is mediated by hemocyte differentiation and phagocytosis

Author:
He Zhang; An-Ran Tan; Peng-Ju Li; Sheng-Ping Lu; Qing-Chen Jia; Shu-Ning Huang; Juan Bai; You-Ming Hou
Source:
Developmental and comparative immunology 2022 v.131 pp. 104380
ISSN:
0145-305X
Subject:
Bacillus thuringiensis; Rhynchophorus ferrugineus; bacteria; hemocytes; immune response; pathogens; pests; phagocytosis; secretion; serotonin
Abstract:
... Red palm weevil, Rhynchophorus ferrugineus, is an invasive and destructive pest that causes serious damages to palm trees. Like other invertebrates, red palm weevil relies solely on its innate immune response to fight invading microbes; by definition, innate immunity lacks adaptive characteristics. However, we show here that priming the red palm weevil larvae with heat-killed Bacillus thuringiensi ...
DOI:
10.1016/j.dci.2022.104380

10. Adrenergic receptor gene expression in bovine leukocytes

Author:
Angela Howell; Natasa Arsic; Robert Brownlie; Philip Griebel
Source:
Developmental and comparative immunology 2022 v.127 pp. 104271
ISSN:
0145-305X
Subject:
cattle; dopamine; eosinophils; epinephrine; gene expression; immune response; neutrophils; norepinephrine; surveys
Abstract:
... The α- and β-adrenergic receptors (ARs) bind the stress hormones epinephrine (E), norepinephrine (NE), and dopamine and activate diverse physiological responses. A lack of information on AR gene expression in leukocytes limits our understanding of how stress alters immune function. Quantitative analyses of AR gene expression was completed for bovine leukocytes. Individual leukocyte lineages and su ...
DOI:
10.1016/j.dci.2021.104271

11. Aflatoxin B1-activated heterophil extracellular traps result in the immunotoxicity to liver and kidney in chickens

Author:
Xinxin Gao; Liqiang Jiang; Jingnan Xu; Wei Liu; Shurou Li; Wenlong Huang; Haiguang Zhao; Zhengtao Yang; Xingang Yu; Zhengkai Wei
Source:
Developmental and comparative immunology 2022 v.128 pp. 104325
ISSN:
0145-305X
Subject:
NAD(P)H oxidase (H2O2-forming); aflatoxin B1; aspergillosis; blood serum; caspase-1; caspase-11; caspase-3; chickens; elastase; energy; glycolysis; hepatocytes; heterophils; histones; histopathology; immunotoxicity; innate immunity; interleukin-6; kidneys; liver; macrophages; necrosis; therapeutics
Abstract:
... Aflatoxin B1 (AFB1) is a mycotoxin with strong toxicity and play a large proportion in aspergillosis. Heterophil extracellular traps (HETs) was considered as an innate immune response of chickens to resist pathogens. AFB1 has been reported to trigger macrophages extracellular traps (METs) in THP-1 cells and RAW264.7 cells, but whether AFB1 could also activate HETs release, and the mechanism underl ...
DOI:
10.1016/j.dci.2021.104325

12. Alpinone: A positive regulator molecule of immune antiviral response in Atlantic salmon kidney cells

Author:
Almendra Benavides; Daniela Gutiérrez; Nadia Epuyao; Brenda Modak; Mónica Imarai; Beatriz Valenzuela
Source:
Developmental and comparative immunology 2022 v.126 pp. 104262
ISSN:
0145-305X
Subject:
Heliotropium; Salmo salar; Salmon isavirus; antiviral properties; disease severity; flavonoids; genes; kidneys; melanoma; mitochondria; quantitative polymerase chain reaction; salmon; transcription (genetics); tumor necrosis factor receptors; viruses
Abstract:
... Alpinone is a flavonoid obtained from the resinous exudate of Heliotropium huascoense. This flavonoid shows antiviral activity against the infectious salmon anemia virus (ISAV), which causes severe disease in farmed Atlantic salmon. Here, we aim to elucidate mechanisms underlying the antiviral effects of the flavonoid. In this regard, we evaluated whether Alpinone can act upregulating the pattern- ...
DOI:
10.1016/j.dci.2021.104262

13. An l-rhamnose-binding lectin from Nile tilapia (Oreochromis niloticus) possesses agglutination activity and regulates inflammation, phagocytosis and respiratory burst of monocytes/macrophages

Author:
Liangliang Mu; Xiaoxue Yin; Weiwei Qi; Jiadong Li; Hao Bai; Nuo Chen; Yanjian Yang; Bei Wang; Jianmin Ye
Source:
Developmental and comparative immunology 2022 v.126 pp. 104256
ISSN:
0145-305X
Subject:
Aeromonas hydrophila; Oreochromis niloticus; Streptococcus agalactiae; agglutination; fish; inflammation; intestines; kidneys; lectins; liver; macrophages; monocytes; phagocytosis; respiratory burst; spleen
Abstract:
... Rhamnose-binding lectins (RBLs), a Ca²⁺-independent lectin family, are widely present in vertebrates and invertebrates, which involve in the innate immune response. However, the functional characterization and related regulation mechanisms of RBLs remain unclear in teleost fish. In this study, an l-rhamnose-binding lectin-like (OnRBL-L) was identified and functionally characterized from Nile tilap ...
DOI:
10.1016/j.dci.2021.104256

14. Analysis of the mast cell expressed carboxypeptidase A3 and its structural and evolutionary relationship to other vertebrate carboxypeptidases

Author:
Srinivas Akula; Lars Hellman; Francesc Xavier Avilés; Sara Wernersson
Source:
Developmental and comparative immunology 2022 v.127 pp. 104273
ISSN:
0145-305X
Subject:
Danio rerio; asthma; bioinformatics; carboxypeptidases; gene duplication; genes; humans; immunology; loci; mast cells; phylogeny; proteinases
Abstract:
... Metallo-carboxypeptidases are exopeptidases with diverse expression and function, found in all kingdoms of life from bacteria to mammals. One of them, the carboxypeptidase A3 (CPA3), has become an important component of the mammalian immune system by its expression in mast cells. Mast cells (MCs) are highly specialized sentinel cells, which store large amounts of bioactive mediators, including CPA ...
DOI:
10.1016/j.dci.2021.104273

15. Antimicrobial and immunomodulatory activity of beta-defensin from the Chinese spiny frog (Quasipaa spinosa)

Author:
Shui-Sheng Yu; Zeng-Hui Zhao; Xiao-Fei Gong; Xiao-Li Fan; Zhi-Hua Lin; Jie Chen
Source:
Developmental and comparative immunology 2022 v.126 pp. 104264
ISSN:
0145-305X
Subject:
Aeromonas hydrophila; Streptococcus iniae; Vibrio harveyi; Vibrio vulnificus; antibacterial properties; cell lines; cell membranes; frogs; immunomodulators; innate immunity; macrophages; mice; monocytes; necrosis; neoplasms; transcriptome
Abstract:
... The β-defensins are important components of the vertebrate innate immune system. While mammalian β-defensins have wide-ranging antibacterial and immunomodulatory activities, those of amphibians remain largely uncharacterised. In this study, β-defensin cDNA was identified from the skin transcriptome of the Chinese spiny frog Quasipaa spinosa. This β-defensin (QS-BD) consists of a signal and a matur ...
DOI:
10.1016/j.dci.2021.104264

16. Antimicrobial peptide gene expression in Atlantic salmon (Salmo salar) seven days post-challenge with Neoparamoeba perurans

Author:
Leisha McGrath; Joan O'Keeffe; Orla Slattery
Source:
Developmental and comparative immunology 2022 v.127 pp. 104287
ISSN:
0145-305X
Subject:
Neoparamoeba; Paramoeba perurans; Salmo salar; amoebic gill disease; cathelicidins; gene expression; microfluidic technology; parasites; quantitative polymerase chain reaction; salmon
Abstract:
... Amoebic gill disease in teleost fish is caused by the marine parasite Neoparamoeba perurans. To date, the role of antimicrobial peptides β-defensins and cathelicidins in this infection have not been explored. Using a high-throughput microfluidics quantitative polymerase chain reaction system (Biomark HD™ by Fluidigm), this study aimed to: firstly, to investigate organ-specific expression of antimi ...
DOI:
10.1016/j.dci.2021.104287

17. Assessing an O-antigen deficient, live attenuated Salmonella Gallinarium strain that is DIVA compatible, environmentally safe, and protects chickens against fowl typhoid

Author:
Amal Senevirathne; Chamith Hewawaduge; John Hwa Lee
Source:
Developmental and comparative immunology 2022 v.133 pp. 104433
ISSN:
0145-305X
Subject:
O-antigens; Salmonella; T-lymphocytes; antibodies; blood serum; chickens; cytotoxicity; fowl typhoid; gene deletion; genes; genotype; histopathology; interleukin-12; interleukin-2; interleukin-4; live vaccines; liver; macrophages; spleen; vaccination
Abstract:
... The objective of the present study was to create a highly attenuated, safe Salmonella Gallinarium (SG) vaccine strain for chicken vaccination against fowl typhoid (FT) diseases. The SG vaccine strain (SGVS) consists of three virulence-related gene deletions, namely, lon, cpxR, and rfaL. The parent strain (SGPS) with Δlon ΔcpxR genotype was utilized as the host strain for in-frame rfaL gene deletio ...
DOI:
10.1016/j.dci.2022.104433

18. Beta interferons from the extant camelids: Unique among eutherian mammals

Author:
Avinash Premraj; Abi George Aleyas; Binita Nautiyal; Thaha Jamal Rasool
Source:
Developmental and comparative immunology 2022 pp. 104443
ISSN:
0145-305X
Subject:
COVID-19 infection; Camelpox virus; Camelus dromedarius; Middle East respiratory syndrome coronavirus; RNA; antiviral agents; camels; genes; immune response; kidneys; pandemic; pathogens
Abstract:
... The COVID-19 pandemic is a wake-up call on the zoonotic viral spillover events and the need to be prepared for future outbreaks. Zoonotic RNA viruses like the Middle East respiratory syndrome coronavirus (MERS-CoV) are potential pathogens that could trigger the next pandemic. Dromedary camels are the only known animal source of MERS-CoV zoonotic infections, but little is known about the molecular ...
DOI:
10.1016/j.dci.2022.104443

19. Bombyx mori cypovirus (BmCPV) induces PINK1-Parkin mediated mitophagy via interaction of VP4 with host Tom40

Author:
Min Zhu; Jun Pan; Mingtian Zhang; Xinyu Tong; Yunshan Zhang; Ziyao Zhang; Zi Liang; Xing Zhang; Xiaolong Hu; Renyu Xue; Guangli Cao; Chengliang Gong
Source:
Developmental and comparative immunology 2022 v.126 pp. 104244
ISSN:
0145-305X
Subject:
Bombyx mori; Cypovirus; Western blotting; autophagosomes; electron microscopy; fluorescence; membrane proteins; midgut; mitochondria; mitochondrial membrane; mitophagy; rapamycin; silkworms; structural proteins; transfection; viruses
Abstract:
... The mechanism by which infection by Bombyx mori cytoplasmic nucleopolyhedrosis virus (BmCPV) causes autophagy has not been studied in detail. Herein we have observed by electron microscopy that infection with BmCPV causes autophagosome and mitochondrial structure damage in Bombyx mori midgut. In BmN cells infected with BmCPV and expressing eGFP-LC3, fluorescence spots and LC3-II levels increased, ...
DOI:
10.1016/j.dci.2021.104244

20. CD9 expression in porcine blood CD4+ T cells delineates two subsets with phenotypic characteristics of central and effector memory cells

Author:
Belén Álvarez; Concepción Revilla; Sara Moreno; Ángeles Jiménez-Marín; Elena Ramos; Paloma Martínez de la Riva; Teresa Poderoso; Juan J. Garrido; Ángel Ezquerra; Javier Domínguez
Source:
Developmental and comparative immunology 2022 v.133 pp. 104431
ISSN:
0145-305X
Subject:
CD4-positive T-lymphocytes; Suid alphaherpesvirus 1; models; monoclonal antibodies; phenotype; swine; vaccination
Abstract:
... In this report, we describe the characterization of a new monoclonal antibody, named 4H5CR4, against porcine CD9. Its use in combination with antibodies to CD4, CD8α, and 2E3 allows to distinguish at least five main CD4⁺ T cell subsets. Analysis on these subsets of CD45RA, CD27, CD29, CD95, CCR7, and SLA-DR markers depicts a progressive model of CD4⁺ T cell development. CD4⁺ 2E3⁺ CD8α⁻ CD9⁻ cells ...
DOI:
10.1016/j.dci.2022.104431