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You searched for: Journal name Developmental and comparative immunology Remove constraint Journal name: Developmental and comparative immunology
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1. Agglutinins of Trypanosoma, Leishmania and Crithidia in insect haemolymph

Author:
Ingram, G.A.; East, J.; Molyneux, D.H.
Source:
Developmental and comparative immunology 1983 v.7 no.4 pp. 649-652
ISSN:
0145-305X
Subject:
Trypanosoma brucei; Crithidia fasciculata; hemolymph; immunity
Abstract:
... The haemolymph of the American cockroach (Periplaneta americana) and the desert locust (Schistocerca gregaria) but not the blowfly (Calliphora erythrocephala) contains agglutinins against the trypanosomatid flagellates Trypanosoma brucei, Leishmania hertigi and Crithidia fasciculata. There was no apparent difference in agglutinin titres between males and females. The preliminary results of selecte ...
DOI:
http://dx.doi.org/10.1016/0145-305X(83)90085-X

2. Fine structural differences in the amoebocytes of Biomphalaria glabrata

Author:
Joky, A.; Matricon-Gondran, M.; Benex, J.
Source:
Developmental and comparative immunology 1983 v.7 no.4 pp. 669-672
ISSN:
0145-305X
Subject:
Schistosoma mansoni; hemocytes; granulocytes; immunity
Abstract:
... Four types of cells have been characterized among “granulocytes” of Biomphalaria glabrata. They differ in size, morphology, organelle content and surface receptors. They are already recognizable when differentiating in the amoebocyte producing organ. ...
DOI:
http://dx.doi.org/10.1016/0145-305X(83)90090-3

3. Lectins and antibodies as molecular probes of molluscan hemocyte surface membranes

Author:
Yoshino, T.P.
Source:
Developmental and comparative immunology 1983 v.7 no.4 pp. 641-644
ISSN:
0145-305X
Subject:
Schistosoma mansoni; immunity; hemocytes
Abstract:
... A variety of carbohydrate-specific lectins and antigen-specific antibodies have been employed to assess the occurrence and topographic distribution of surface membrane determinants on hemocytes of two stocks of the snail, Biomphalariaglabrata, which differ in their susceptibilities to larval blood fluke (Schistosomamansoni) infections. Using fluorescence labeling methods, several “classes” of hemo ...
DOI:
http://dx.doi.org/10.1016/0145-305X(83)90083-6

4. The circulating hemocytes of Culex pipiens and Aedes aegypti: Cytology, histochemistry, hemograms and functions

Author:
Drif, L.; Brehelin, M.
Source:
Developmental and comparative immunology 1983 v.7 no.4 pp. 687-690
ISSN:
0145-305X
Subject:
Steinernema carpocapsae; hemocytes; immunity
Abstract:
... The hemocyte types of Culexpipiens and Aedesaegypti larvae are described. Plasmatocytes and oenocytoids are present and are involved in phagocytosis and melanin production, respectively. Comparisons are made with the findings of previous authors and together with modifications in the hemogram following parasitization by the nematode, Neoplectacarpocapsae, indicate that the hemocytes are also invol ...
DOI:
http://dx.doi.org/10.1016/0145-305X(83)90094-0

5. The immune response of Lacerta viridis to Leishmania agamae

Author:
Ingram, G.A.; Molyneux, D.H.
Source:
Developmental and comparative immunology 1983 v.7 no.4 pp. 717-718
ISSN:
0145-305X
Subject:
labeling techniques; antigens; serodiagnosis; enzymes; immunoglobulins; agglutination; blood
Abstract:
... Lacertaviridis produced relatively heat-stable, dithiothreitol-sensitive, non-precipitating antibodies with β2-electrophoretic mobility following exposure to Leishmaniaagamae promastigotes but no histopathological or clinical signs of infection were seen. Significant four-and two-fold increases in the mean lysozyme and protein levels respectively were found in immune sera. Immunoenzyme techniques ...
DOI:
http://dx.doi.org/10.1016/0145-305X(83)90105-2

6. Signal transduction pathways activated by engaging immunoglobulin Fc receptors on chicken heterophils

Author:
Kogut, M.H.; Genovese, K.J.; Nisbet, D.J.
Source:
Developmental and comparative immunology 2001 v.25 no.7 pp. 639-646
Subject:
chickens; heterophils; phagocytosis; signal transduction; immunoglobulins; chemiluminescence; beta-glucuronidase; enzyme activity; G-proteins; protein kinase C; calcium channel blockers
Abstract:
... In the present studies, we initiated experiments to identify the signal transduction factors involved in activating phagocytosis, oxidative burst, and degranulation following the binding of IgG-opsonized SE to Fc receptors on the surface of avian heterophils. Peripheral blood heterophils were isolated and exposed to known inhibitors of signal transduction pathways for either 20 min (chelerythine, ...
Handle:
10113/3176
DOI:
10.1016/S0145-305X(01)00026-X
PubMed:
11472785

7. Differential effects of age on chicken heterophil functional activation by recombinant chicken interleukin-2

Author:
Kogut, M.; Rothwell, L.; Kaiser, P.
Source:
Developmental and comparative immunology 2002 v.26 no.9 pp. 817-830
Subject:
chickens; Salmonella Enteritidis; recombinant fusion proteins; cell-mediated immunity; interleukin-2; neutrophils; monoclonal antibodies; immune response; phagocytosis; heterophils; antibacterial properties
Abstract:
... Interleukin-2 (IL-2) exercises an array of biological effects on many cells including the functional activation of cells of the innate immune response. Heterophils, the avian equivalent of the neutrophil, function as professional phagocytes to aid in regulation of innate host defenses. The objective of the present studies was to examine the effects of recombinant chicken IL-2 (rChIL-2) on function ...
Handle:
10113/4258
DOI:
10.1016/S0145-305X(02)00040-X
PubMed:
12377221

8. CpG-induced immunomodulation and intracellular bacterial killing in a chicken macrophage cell line

Author:
Xie, Hang; Raybourne, Richard B.; Babu, Uma S.; Lillehoj, Hyun S.; Heckert, Robert A.
Source:
Developmental and comparative immunology 2003 v.27 no.9 pp. 823-834
Subject:
chickens; nitric oxide synthase; nitric oxide; Salmonella Enteritidis; macrophages; interleukin-6; immune response; cell lines
Abstract:
... The immunostimulatory properties of synthetic CpG oligodeoxynucleotides (ODNs) have been studied in various mammalian models including humans and mice. However, little was known about effects of CpG ODNs on immune responses of chickens, a common avian species with important economical value in the poultry industry. In the present study, two CpG ODNs, 2006 and 1826, which show immunomodulating prop ...
Handle:
10113/48748
DOI:
10.1016/S0145-305X(03)00079-X

9. Oxidative burst mediated by toll like receptors (TLR) and CD14 on avian heterophils stimulated with bacterial toll agonists

Author:
Farnell, M.B.; Crippen, T.L.; He, H.; Swaggerty, C.L.; Kogut, M.H.
Source:
Developmental and comparative immunology 2003 v.27 no.5 pp. 423-429
Subject:
chickens; Staphylococcus aureus; lipopolysaccharides; humans; Salmonella Enteritidis; polyclonal antibodies; immune response; rats; receptors; heterophils
Abstract:
... Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns (PAMPs) such as lipopolysaccharide (LPS) and lipoteichoic acid (LTA), which are found in the cell walls of gram-negative and gram-positive bacteria, respectively. This study was conducted to determine if TLRs are present on chicken heterophils and if these receptors mediate oxidative burst. Heterophils isolated from neonat ...
Handle:
10113/2804
DOI:
10.1016/S0145-305X(02)00115-5
PubMed:
12631524

10. Identification and characterization of chicken interleukin-16 cDNA

Author:
Min, Wongi; Lillehoj, Hyun Soon
Source:
Developmental and comparative immunology 2004 v.28 no.2 pp. 153–162
Subject:
chickens; messenger RNA; coccidiosis; Eimeria; chemoattractants; complementary DNA; open reading frames; interleukins; lymphocytes; amino acid sequences; gene expression; intestines; chemotaxis
Abstract:
... Interleukin-16 is an inflammatory cytokine synthesized as a precursor protein (pro-IL-16). Based on sequence data from an EST cDNA library prepared from intestinal intraepithelial lymphocytes of Eimeria-infected chickens, we identified a cDNA that contained a full-length open reading frame of pro-IL-16. The encoded protein, predicted to consist of 607 amino acids, showed 86% sequence identity to d ...
Handle:
10113/47315
DOI:
10.1016/S0145-305X(03)00133-2

11. Analysis of a catfish gene resembling interleukin-8: cDNA cloning, gene structure, and expression after infection with Edwardsiella ictaluri

Author:
Chen, Liqiao; He, Chongbo; Baoprasertkul, Puttharat; Xu, Peng; Li, Ping; Serapion, Jerry; Waldbieser, Geoff; Wolters, William; Liu, Zhanjiang
Source:
Developmental and comparative immunology 2005 v.29 no.2 pp. 135-142
Subject:
sequence analysis; alternative splicing; Edwardsiella ictaluri; complementary DNA; bacteremia; animal pathogenic bacteria; transcription (genetics); amino acid sequences; freshwater fish; Ictalurus furcatus; fish diseases; catfish; Ictalurus punctatus; molecular cloning; immune response; nucleotide sequences; bacterial infections; interleukin-8
Abstract:
... Chemokines are important mediators for innate immunity involved in recruitment, activation and adhesion of a variety of leukocyte types to inflammatory foci. While almost all chemokines have been identified from mammals, only a handful of fish chemokines have been identified. Here we report molecular cloning, sequence analysis, and expression of a channel catfish gene resembling interleukin-8 (IL- ...
Handle:
10113/40783
DOI:
10.1016/j.dci.2004.06.011
PubMed:
15450753

12. CpG-oligodeoxynucleotide-stimulated chicken heterophil degranulation is serum cofactor and cell surface receptor dependent

Author:
He, Haiqi; Lowry, Virginia K.; Ferro, Pamela J.; Kogut, Michael H.
Source:
Developmental and comparative immunology 2005 v.29 no.3 pp. 255-264
Subject:
bacterial infections; chickens; heterophils; immune response; oligonucleotides; immunostimulants; clathrin; receptors; endocytosis; endosomes
Abstract:
... Synthetic oligodeoxynucleotide containing unmethylated CpG motif (CpG-ODN) is immune stimulatory to chicken heterophils. Recognition of CpG-ODN by chicken heterophils leads to the mobilization and release of granules. This CpG-ODN-induced heterophil degranulation was chicken serum (CS)-dependent. Heat-denaturation and membrane filtration of CS revealed that the active serum cofactor(s) was likely ...
Handle:
10113/39296
DOI:
10.1016/j.dci.2004.07.005
PubMed:
15572073

13. Expression and function of Toll-like receptors in chicken heterophils

Author:
Kogut, Michael H.; Iqbal, Muhammad; He, Haiqi; Philbin, Victoria; Kaiser, Pete; Smith, Adrian
Source:
Developmental and comparative immunology 2005 v.29 no.9 pp. 791-807
Subject:
chickens; messenger RNA; cytokines; signal transduction; gene expression; resistance mechanisms; protein phosphorylation; immune response; phosphotransferases (kinases); receptors; agonists; heterophils
Abstract:
... The heterophil is the major polymorphonuclear cell in birds with a functional capacity akin to that of the mammalian neutrophil. Herein, we demonstrate that heterophils constitutively express TLR1/6/10, TLR2 type 1, TLR2 type 2, TLR3, TLR4, TLR5, and TLR7 mRNA. Furthermore, TLR agonists, including flagellin (from Salmonella typhimurium, FGN), peptidoglycan (from Staphylococcus aureus, PGN), ultra- ...
Handle:
10113/37463
DOI:
10.1016/j.dci.2005.02.002
PubMed:
15936435

14. MHC RFLP analyses in channel catfish full-sibling families: identification of the role of MHC molecules in spontaneous allogeneic cytotoxic responses

Author:
Quiniou, Sylvie M.A.; Wilson, Melanie; Bengten, Eva; Waldbieser, Geoffrey C.; Clem, L. William; Miller, Norman W.
Source:
Developmental and comparative immunology 2005 v.29 no.5 pp. 457-467
Subject:
Ictalurus punctatus; catfish; freshwater fish; major histocompatibility complex; gene segregation; linkage (genetics); natural killer cells; restriction fragment length polymorphism; immune response; cytotoxicity; fluorescence in situ hybridization
Abstract:
... Genes encoding MHC class I and II molecules have been identified in a number of fish species, including the channel catfish, but there is still a dearth of knowledge concerning their functional roles in teleost immune responses. This has in part been due to a lack of appropriate MHC class I and II matched and mismatched animals. To identify such animals, MHC segregation and linkage studies in the ...
Handle:
10113/39298
DOI:
10.1016/j.dci.2004.08.008
PubMed:
15707666

15. Recombinant chicken IL-6 does not activate heterophils isolated from day-old chickens in vitro

Author:
Ferro, Pamela J.; Swaggerty, Christina L.; He, Haiqi; Rothwell, Lisa; Kaiser, Pete; Kogut, Michael H.
Source:
Developmental and comparative immunology 2005 v.29 no.4 pp. 375-383
Subject:
chickens; Salmonella Enteritidis; recombinant fusion proteins; interleukin-6; immune response; phagocytosis; heterophils; chemotaxis; antibacterial properties
Abstract:
... Pro-inflammatory cytokines are produced as part of innate immunity. Increased resistance to extraintestinal Salmonella enteritidis (SE) has been associated with an increase in heterophil pro-inflammatory cytokine gene expression. Invasion of chicken epithelial cells by SE induces an 8- to 10-fold increase in interleukin (IL) -6 production. Infection with SE induces an influx of heterophils to the ...
Handle:
10113/39295
DOI:
10.1016/j.dci.2004.08.002
PubMed:
15859240

16. Differential reactive oxygen and nitrogen production and clearance of Salmonella serovars by chicken and mouse macrophages

Author:
Babu, Uma S.; Gaines, Dennis W.; Lillehoj, Hyun; Raybourne, Richard B.
Source:
Developmental and comparative immunology 2006 v.30 no.10 pp. 942-953
Subject:
chickens; nitric oxide; Salmonella Enteritidis; macrophages; Salmonella Typhimurium; mice; food pathogens; salmonellosis; cultured cells; immune response; superoxide anion; phagocytosis; interferons
Abstract:
... The objective of the present study was to compare the uptake and killing of Salmonella serovars by murine and avian macrophage cell lines. We used Salmonella enterica serovars Enteritidis (SE338) and Typhimurium (SR11) for this study. Uptake of green fluorescent protein-labeled bacteria was measured using flow cytometry. Cell sorting and plating of viable infected macrophages demonstrated that bac ...
Handle:
10113/28973
DOI:
10.1016/j.dci.2005.12.001
PubMed:
16427126

17. Immunomodulatory effects of b-glucan on neutrophil function in fathead minnows (Pimephales promelas Rafinesque, 1820)

Author:
Palic, Dusan; Andreasen, Claire B.; Herolt, Dawn M.; Menzel, Bruce W.; Roth, James A.
Source:
Developmental and comparative immunology 2006 v.30 no.9 pp. 817-830
Subject:
Pimephales promelas; beta-glucans; dietary carbohydrate; neutrophils; Saccharomyces cerevisiae; bakers yeast; barley starch; fish culture; fish feeding
Abstract:
... Stimulatory effects of yeast β-1,3-1,6-glucans on neutrophils have long been recognized, but effects of glucans on degranulation of primary granules in fish neutrophils have not been previously reported. Neutrophil function was monitored during in vitro and in vivo application of glucans to non- (NS), acute- (AS) and chronically stressed (CS) fish. β-Glucan proved to be a strong and quick (80%, 2 ...
Handle:
10113/28970
DOI:
10.1016/j.dci.2005.11.004

18. Molecular cloning and characterization of chicken lipopolysaccharide-induced TNF-a factor (LITAF)

Author:
Hong, Yeong Ho; Lillehoj, Hyun S.; Lee, Sung Hyen; Park, Dong Woon; Lillehoj, Erik P.
Source:
Developmental and comparative immunology 2006 v.30 no.10 pp. 919-929
Subject:
lipopolysaccharides; Salmonella Typhimurium; Eimeria acervulina; Eimeria maxima; bacterial toxins; macrophages; tissue distribution; gene expression regulation; endotoxins; Escherichia coli; transcription factors; coccidiosis; complementary DNA; cytotoxicity; transcription (genetics); tumor necrosis factor-alpha; amino acid sequences; molecular cloning; immune response; chickens; cultured cells; Eimeria tenella; anticarcinogenic activity
Abstract:
... The inflammatory response to parasites, bacteria, and viruses is mediated by multiple host factors. TNF-α is one of the most pleiotropic cytokines in mammals, but has yet to be identified in avian species. In the current study, we isolated a full-length cDNA encoding the chicken homologue of LPS-induced TNF-α factor (LITAF), transcription factor, with an open reading frame of 148 amino acids and a ...
Handle:
10113/28976
DOI:
10.1016/j.dci.2005.12.007

19. Channel catfish, Ictalurus punctatus, CD4-like molecules

Author:
Edholm, Eva-Stina; Stafford, James L.; Quiniou, Sylvie M.; Waldbieser, Geoff; Miller, Norman W.; Bengten, Eva; Wilson, Melanie
Source:
Developmental and comparative immunology 2007 v.31 no.2 pp. 172-187
Subject:
sequence analysis; messenger RNA; complementary DNA; lymphocyte antigens; transcription (genetics); amino acid sequences; freshwater fish; nucleotide sequences; catfish; Ictalurus punctatus; molecular cloning; gene expression regulation; CD8-positive T-lymphocytes
Abstract:
... Two CD4-like (CD4L) molecules have been identified in channel catfish, Ictalurus punctatus. Although phylogenetically related to other vertebrate CD4 molecules, they exhibit only 19% amino acid identity to each other. IpCD4L-1 encodes a predicted protein containing four immunoglobulin domains, a transmembrane region and a cytoplasmic tail containing a p56lck binding site. In contrast, IpCD4L-2 enc ...
Handle:
10113/29057
DOI:
10.1016/j.dci.2006.05.012
PubMed:
16844219

20. Evolutionary history of the ABCB2 genomic region in teleosts

Author:
Palti, Y.; Rodriguez, M.F.; Gahr, S.A.; Hansen, J.D.
Source:
Developmental and comparative immunology 2007 v.31 no.5 pp. 483-498
ISSN:
0145-305X
Abstract:
... Gene duplication, silencing and translocation have all been implicated in shaping the unique genomic architecture of the teleost MH regions. Previously, we demonstrated that trout possess five unlinked regions encoding MH genes. One of these regions harbors ABCB2 which in all other vertebrate classes is found in the MHC class II region. In this study, we sequenced a BAC contig for the trout ABCB2 ...
Handle:
10113/10634
DOI:
10.1016/j.dci.2006.07.010

21. Functional characterization of tumor necrosis factor superfamily 15 (TNFSF15) induced by lipopolysaccharides and Eimeria infection

Author:
Park, S.S.; Lillehoj, H.S.; Hong, Y.H.; Lee, S.H.
Source:
Developmental and comparative immunology 2007 v.31 no.9 pp. 934-944
Subject:
chickens; tumor necrosis factors; gene expression; messenger RNA; coccidiosis; Eimeria tenella; Eimeria acervulina; Eimeria maxima; lymphocytes; spleen; bursa of Fabricius; inflammation; host-pathogen relationships; complementary DNA; nucleotide sequences; amino acid sequences; macrophages
Abstract:
... A full-length cDNA encoding chicken tumor necrosis factor superfamily 15 (TNFSF15) was isolated and its functional role was investigated. TNFSF15 transcripts were primarily expressed in spleen, liver, intestinal intraepithelial lymphocytes (IEL), peripheral blood lymphocytes and bursa. In vitro infection of HTC macrophages with three species of Eimeria sporozoites induced TNFSF15 gene expression. ...
Handle:
10113/14289
DOI:
10.1016/j.dci.2006.12.010
PubMed:
17349692

22. Bovine WC1⁻γδ cells incubated with IL-15 express the natural cytotoxicity receptor CD335 (NKp46) and produce IFN-γ in response to exogenous IL-12 and IL-18

Author:
Johnson, Wendell C.; Bastos, Reginaldo G.; Davis, William C.; Goff, Will L.
Source:
Developmental and comparative immunology 2008 v.32 no.8 pp. 1002-1010
Subject:
interleukin-12; T-lymphocytes; lymphocyte antigens; cattle; interferons
Abstract:
... The γδT cells of ruminants are believed to participate in innate immunity and have been described with regulatory, inflammatory and cytotoxic functions. Here we describe a subset of CD3+ TcR1+ WC1- γδT cells expressing CD335 (NKp46), classically associated with CD3- natural killer (NK) cells, as a consequence of incubation with IL-15. This population, undetectable at the time of collection, develo ...
Handle:
10113/36261
DOI:
10.1016/j.dci.2008.01.011

23. Identification and characterization of an alternatively spliced variant of the MHC class I-related porcine neonatal Fc receptor for IgG

Author:
Ye, Lilin; Tuo, Wenbin; Liu, Xindong; Simister, Neil E.; Zhu, Xiaoping
Source:
Developmental and comparative immunology 2008 v.32 no.8 pp. 966-979
Subject:
swine; major histocompatibility complex; alternative splicing; messenger RNA; immunoglobulin G; amino acid sequences; nucleotide sequences
Abstract:
... The neonatal Fc receptor for immunoglobulin G (IgG) (FcRn) functions to transport maternal IgG to the fetal/neonatal animals and protects IgG from catabolism. The present study identified two pFcRn cDNAs (1.071 and 0.795 kb) from intestinal epithelial cells. The corresponding mRNA transcripts were detected in porcine kidney cell line LLC-PK1, peripheral blood mononuclear cells, and porcine tissues ...
Handle:
10113/35932
DOI:
10.1016/j.dci.2008.01.008
PubMed:
18321573
PubMed Central:
PMC2464570

24. Phospholipase C, phosphatidylinositol 3-kinase, and intracellular [Ca2+] mediate the activation of chicken HD11 macrophage cells by CpG oligodeoxynucleotide

Author:
He, Haiqi; Genovese, Kenneth J.; Nisbet, David J.; Kogut, Michael H.
Source:
Developmental and comparative immunology 2008 v.32 no.10 pp. 1111-1118
Subject:
oligonucleotides; phospholipase C; phosphatidylinositol 3-kinase; enzyme activation; calcium; signal transduction; macrophages; chickens; immune response; nitric oxide; protein kinase C
Abstract:
... The activation of phospholipases is one of the earliest key events in receptor-mediated cellular responses to a number of extracellular signaling molecules. Oligodeoxynucleotides containing CpG motifs (CpG ODN) mimic microbial DNA and are immunostimulatory to most vertebrate species. In the present study, we used the production of nitric oxide (NO) as an indicator to evaluate the involvement of th ...
Handle:
10113/32355
DOI:
10.1016/j.dci.2008.02.009
PubMed:
18403015

25. Bacterial, but not baculoviral infections stimulate Hemolin expression in noctuid moths

Author:
Terenius, Olle; Popham, Holly J.R.; Shelby, Kent S.
Source:
Developmental and comparative immunology 2009 v.33 no.11 pp. 1176-1185
Subject:
Helicoverpa zea; insect viruses; sequence analysis; protein synthesis; Micrococcus luteus; complementary DNA; insect immunity; immunoglobulins; Helicoverpa zea single nucleopolyhedrovirus; immune response; Heliothis virescens; gene expression regulation; bacterial infections
Abstract:
... Lepidopteran larvae are regularly infected by baculoviruses during feeding on infected plants. The differences in sensitivity to these infections can be substantial, even among closely related species. For example, the noctuids Cotton bollworm (Helicoverpa zea) and Tobacco budworm (Heliothis virescens), have a 1000-fold difference in sensitivity to Autographa californica multiple nucleopolyhedrovi ...
Handle:
10113/36305
DOI:
10.1016/j.dci.2009.06.009
PubMed:
19540262

26. Expression profiles of cloned channel catfish (Ictalurus punctatus) lymphoid cell lines and mixed lymphocyte cultures

Author:
Majji, S.; Thodima, V.; Arnizaut, A.; Deng, Y.; May, W.; Sittman, D.; Waldbieser, G.C.; Hanson, L.; Cuchens, M.A.; Bengten, E.; Chinchar, V.G.
Source:
Developmental and comparative immunology 2009 v.33 no.2 pp. 224-234
Subject:
microarray technology; lymphocytes; freshwater fish; cultured cells; catfish; Ictalurus punctatus; gene expression regulation
Abstract:
... Clonal channel catfish lymphoid cell lines and mixed lymphocyte cultures (MLCs) have proven extremely useful in examining immune responses at the cellular and molecular levels. To date clonal catfish cell lines and MLCs have been biologically and phenotypically characterized using a variety of techniques including reverse transcription polymerase chain reaction (RT-PCR), as well as Northern and So ...
Handle:
10113/22925
DOI:
10.1016/j.dci.2008.08.010
PubMed:
18824023

27. Molecular genetics of the swine major histocompatibility complex, the SLA complex

Author:
Lunney, Joan K.; Ho, Chak-Sum; Wysocki, Michael; Smith, Douglas M.
Source:
Developmental and comparative immunology 2009 v.33 no.3 pp. 362-374
Subject:
swine; major histocompatibility complex; genomics; chromosome mapping; genetic polymorphism; genetic variation; genetic recombination; gene expression regulation; immune response
Abstract:
... The swine major histocompatibility complex (MHC) or swine leukocyte antigen (SLA) complex is one of the most gene-dense regions in the swine genome. It consists of three major gene clusters, the SLA class I, class III and class II regions, that span approximately 1.1, 0.7 and 0.5 Mb, respectively, making the swine MHC the smallest among mammalian MHC so far examined and the only one known to span ...
Handle:
10113/22905
DOI:
10.1016/j.dci.2008.07.002
PubMed:
18760302

28. Identification and characterization of matrix metalloproteinase-13 sequence structure and expression during embryogenesis and infection in channel catfish (Ictalurus punctatus)

Author:
Jiang, Yanliang; Abernathy, Jason W.; Peatman, Eric; Liu, Hong; Wang, Shaolin; Xu, De-Hai; Kucuktas, Huseyin; Klesius, Phillip; Liu, Zhanjiang
Source:
Developmental and comparative immunology 2010 v.34 no.5 pp. 590-597
Subject:
messenger RNA; Edwardsiella ictaluri; complementary DNA; gene expression; genes; nucleotide sequences; Ictalurus punctatus; collagenase; embryogenesis; gene expression regulation; bacterial infections
Abstract:
... Matrix metalloproteinase-13 (MMP-13), referred to as collagenase-3, is a proteolytic enzyme that plays a key role in degradation and remodelling of host extracellular matrix proteins. The objective of this study was to characterize the MMP-13 gene in channel catfish, and to determine its pattern of expression in various healthy tissues and during embryogenesis. Since MMP-13 has been shown to have ...
Handle:
10113/43622
DOI:
10.1016/j.dci.2010.01.001
PubMed:
20060415

29. 17β-Estradiol regulates gilthead seabream professional phagocyte responses through macrophage activation

Author:
S. Liarte; E. Chaves-Pozo; E. Abellán; J. Meseguer; V. Mulero; A. García-Ayala
Source:
Developmental and comparative immunology 2011 v.35 no.1 pp. 19-27
ISSN:
0145-305X
Subject:
Sparus aurata; autocrine signaling; chemokines; estradiol; estrogen receptors; gene expression; genes; granulocytes; kidneys; lymphocytes; macrophage activation; macrophages; mammals; migratory behavior; phagocytosis; reactive oxygen species
Abstract:
... In mammals, estrogens regulate the immune system, either directly or indirectly via several leukocyte types through autocrine/paracrine mechanisms. In the gilthead seabream (Sparus aurata L.) gonad, an intensive remodeling process accompanied by the massive infiltration of acidophilic granulocytes (AG) is partially triggered by 17β-estradiol (E₂). Once AG infiltrated the gonad, show impaired activ ...
DOI:
10.1016/j.dci.2010.07.007

30. Activation of an innate immune response in large numbers of permeabilized Drosophila embryos

Author:
Shiva Seyedoleslami Esfahani; Ylva Engström
Source:
Developmental and comparative immunology 2011 v.35 no.3 pp. 263-266
ISSN:
0145-305X
Subject:
Drosophila; adults; antimicrobial peptides; genes; immune response; innate immunity; larvae; mutants; signal transduction
Abstract:
... Innate immunity in Drosophila involves the inducible expression and synthesis of antimicrobial peptides. We have previously shown that not only Drosophila larvae and adults, but also embryos, are capable of mounting an immune response after injection of bacterial substances. To simplify genetic dissection of the signaling pathways involved in immune-gene regulation we developed a procedure for per ...
DOI:
10.1016/j.dci.2010.11.002

31. Activation-induced cytidine deaminase structure and functions: A species comparative view

Author:
Vasco M. Barreto; Brad G. Magor
Source:
Developmental and comparative immunology 2011 v.35 no.9 pp. 991-1007
ISSN:
0145-305X
Subject:
Petromyzontiformes; cytidine deaminase; humans; humoral immunity; immunoglobulin genes; mice; models; sheep
Abstract:
... In the ten years since the discovery of activation-induced cytidine deaminase (AID) there has been considerable effort to understand the mechanisms behind this enzyme's ability to target and modify immunoglobulin genes leading to somatic hypermutation and class switch recombination. While the majority of research has focused on mouse and human models of AID function, work on other species, from la ...
DOI:
10.1016/j.dci.2011.02.005

32. Acute phase response in Chinese soft-shelled turtle (Trionyx sinensis) with Aeromonas hydrophila infection

Author:
Xiuxia Zhou; Lu Wang; Hong Feng; Qionglin Guo; Heping Dai
Source:
Developmental and comparative immunology 2011 v.35 no.4 pp. 441-451
ISSN:
0145-305X
Subject:
cathepsin L; mammals; promoter regions; acute phase proteins; open reading frames; spleen; turtles; introns; bacterial infections; reptile culture; messenger RNA; albumins; blood serum; complementary DNA; Aeromonas hydrophila; amino acid sequences; immune response; kidneys; exons; birds; complement; liver; Pelodiscus sinensis; binding sites; amyloid; bacteria
Abstract:
... Chinese soft-shelled turtle (Trionyx sinensis) is an important culture reptile. However, little is known about its acute phase response (APR) caused by bacteria. Serum amyloid A (SAA) is a major acute phase protein (APP). In this study, a turtle SAA homologue was identified and described in reptiles. The full-length cDNA of turtle SAA was 554bp and contained a 381bp open reading frame (ORF) coding ...
DOI:
10.1016/j.dci.2010.11.011

33. Advances in fish vaccine delivery

Author:
Karen P. Plant; Scott E. LaPatra
Source:
Developmental and comparative immunology 2011 v.35 no.12 pp. 1256-1262
ISSN:
0145-305X
Subject:
aquaculture; disease prevention; fish; labor; vaccination; vaccines
Abstract:
... Disease prevention is essential to the continued development of aquaculture around the world. Vaccination is the most effective method of combating disease and currently there are a number of vaccines commercially available for use in fish. The majority of aquatic vaccines are delivered by injection, which is by far the most effective method when compared to oral or immersion deliveries. However i ...
DOI:
10.1016/j.dci.2011.03.007

34. Analysis of the antimicrobial responses of primary phagocytes of the goldfish (Carassius auratus L.) against Mycobacterium marinum

Author:
Leon Grayfer; Jordan W. Hodgkinson; Miodrag Belosevic
Source:
Developmental and comparative immunology 2011 v.35 no.11 pp. 1146-1158
ISSN:
0145-305X
Subject:
Carassius auratus; Mycobacterium marinum; NAD(P)H oxidase (H2O2-forming); NADP (coenzyme); cytokine receptors; gene expression regulation; genes; goldfish; host-pathogen relationships; hosts; humans; interleukin-10; macrophages; messenger RNA; monocytes; mycobacterial diseases; nitric oxide; oxygen production; tumor necrosis factor-alpha
Abstract:
... The slow growth rate of Mycobacterium spp. that infect humans coupled with a lack of reliable in vitro infection model systems has hindered the progress of research in host cell–mycobacteria interactions. Recent studies have utilized the relatively fast growing Mycobacterium marinum to examine the host–pathogen interface in natural fish hosts. Here we describe the use of primary goldfish monocyte ...
DOI:
10.1016/j.dci.2011.04.007

35. Antimicrobial mechanisms of fish leukocytes

Author:
Aja M. Rieger; Daniel R. Barreda
Source:
Developmental and comparative immunology 2011 v.35 no.12 pp. 1238-1245
ISSN:
0145-305X
Subject:
antimicrobial peptides; cell viability; death; enzymes; fish; homeostasis; immunomodulation; leukocytes; pathogens; tissues; toxicity
Abstract:
... Early activation and coordination of innate defenses are critical for effective responses against infiltrating pathogens. Rapid engagement of immune cells provides a critical first line of defense soon after pathogen infiltration. Activation leads to a well-orchestrated set of events that sees the induction and regulation of intracellular and extracellular antimicrobial defenses. An array of regul ...
DOI:
10.1016/j.dci.2011.03.009

36. Antisense approaches for elucidating ranavirus gene function in an infected fish cell line

Author:
D.S. Whitley; R.C. Sample; A.R. Sinning; J. Henegar; V.G. Chinchar
Source:
Developmental and comparative immunology 2011 v.35 no.9 pp. 937-948
ISSN:
0145-305X
Subject:
DNA; DNA-directed RNA polymerase; Frog virus 3; cell culture; coat proteins; fish; genes; immune evasion; immune response; methyltransferases; oligonucleotides; small interfering RNA; virulence; viruses
Abstract:
... Viral virulence/immune evasion strategies and host anti-viral responses represent different sides of the continuing struggle between virus and host survival. To identify virus-encoding molecules whose function is to subvert or blunt host immune responses, we have adapted anti-sense approaches to knock down the expression of specific viral gene products. Our intention is to correlate knock down wit ...
DOI:
10.1016/j.dci.2010.12.002

37. l- Arginine enhances immunity to parasitoids in Drosophila melanogaster and increases NO production in lamellocytes

Author:
Alex R. Kraaijeveld; Naji P. Elrayes; Hansjürgen Schuppe; Philip L. Newland
Source:
Developmental and comparative immunology 2011 v.35 no.8 pp. 857-864
ISSN:
0145-305X
Subject:
Asobara tabida; Drosophila melanogaster; arginine; eggs; encapsulation; hemocytes; immunity; larvae; nitric oxide; nitric oxide synthase; nutrition; parasitism; parasitoids
Abstract:
... Drosophila melanogaster was used as a model system to explore the link between nutrition and immunity, and to investigate the role of nitric oxide (NO) in enhancing immunity following dietary enhancement with l-arginine. First, we show that adding l-arginine to the food medium increases the ability of D. melanogaster larvae to encapsulate the eggs of the parasitoid Asobara tabida. Secondly, we sho ...
DOI:
10.1016/j.dci.2011.03.019

38. Avian-specific TLRs and downstream effector responses to CpG-induction in chicken macrophages

Author:
Ceren Ciraci; Susan J. Lamont
Source:
Developmental and comparative immunology 2011 v.35 no.3 pp. 392-398
ISSN:
0145-305X
Subject:
Escherichia; RNA interference; Salmonella; Toll-like receptors; chickens; gene expression; genes; lipopeptides; lipopolysaccharides; macrophages; mammals; messenger RNA; oligodeoxyribonucleotides; small interfering RNA
Abstract:
... Chickens possess toll-like receptor (TLR15), a pattern recognition receptor (PRR) absent in mammals. We characterized the regulation and mechanism of CpG responsiveness via TLRs in chicken macrophage HD11 cells. TLR15 was significantly upregulated after induction with B- and C-type CpG oligonucleotides (ODN), tripalmitoylated lipopeptide (PAM3CSK4), Escherichia coli- and Salmonella enteritidis-der ...
DOI:
10.1016/j.dci.2010.11.012

39. Biochemical characterization of evasion from peptidoglycan recognition by Staphylococcus aureusd-alanylated wall teichoic acid in insect innate immunity

Author:
Kenji Kurokawa; Ji-Hee Gong; Kyoung-Hwa Ryu; Lili Zheng; Jun-Ho Chae; Min-Su Kim; Bok Luel Lee
Source:
Developmental and comparative immunology 2011 v.35 no.8 pp. 835-839
ISSN:
0145-305X
Subject:
Drosophila; Staphylococcus aureus; Tenebrio molitor; cell free system; chemical bonding; crosslinking; humoral immunity; innate immunity; insects; larvae; peptidoglycans; proteolysis
Abstract:
... We recently reported that d-alanylation of Staphylococcus aureus wall teichoic acid (WTA) mitigates an induction of the Toll-mediated humoral response in Drosophila by interfering with peptidoglycan (PG) recognition by PG recognition protein-SA (PGRP-SA). Here, we investigated the mode of this interference by using an in vitro cell free system from larvae of the coleoptran insect Tenebrio molitor. ...
DOI:
10.1016/j.dci.2011.03.001

40. The C1q domain containing proteins of the Mediterranean mussel Mytilus galloprovincialis: A widespread and diverse family of immune-related molecules

Author:
Marco Gerdol; Chiara Manfrin; Gianluca De Moro; Antonio Figueras; Beatriz Novoa; Paola Venier; Alberto Pallavicini
Source:
Developmental and comparative immunology 2011 v.35 no.6 pp. 635-643
ISSN:
0145-305X
Subject:
Chordata; Mytilus galloprovincialis; adaptive immunity; apoptosis; binding properties; cell adhesion; cell differentiation; complement; gene duplication; genes; inflammation; lectins; mussels; surveys
Abstract:
... The key component of the classical complement pathway C1q is regarded as a major connecting link between innate and acquired immunity due to the highly adaptive binding properties of its trimeric globular domain gC1q. The gC1q domain also characterizes many non-complement proteins involved in a broad range of biological processes including apoptosis, inflammation, cell adhesion and cell differenti ...
DOI:
10.1016/j.dci.2011.01.018

41. CXCL8 of Scophthalmus maximus: Expression, biological activity and immunoregulatory effect

Author:
Yong-Hua Hu; Ling Chen; Li Sun
Source:
Developmental and comparative immunology 2011 v.35 no.10 pp. 1032-1039
ISSN:
0145-305X
Subject:
Scophthalmus maximus; alanine; amino acid sequences; bacterial infections; bioactive properties; cell culture; chemotaxis; dose response; gene expression regulation; inflammation; interleukin-8; kidneys; lymphocytes; macrophages; mutation; neutrophils; pathogens; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; subunit vaccines; tumor necrosis factor-alpha; turbot; vaccination
Abstract:
... CXCL8, or interleukin-8, is a CXC chemokine that promotes neutrophil migration in response to inflammatory stimuli. In this study, we identified and analyzed a CXCL8 orthologue, SmCXCL8, from turbot (Scophthalmus maximus). The deduced amino acid sequence of SmCXCL8 is 99-residue in length and shares 52–83% overall identities with the lineage 1 CXCL8 of a number of teleost. SmCXCL8 possesses a CXC ...
DOI:
10.1016/j.dci.2011.04.002

42. Candidate innate immune system gene expression in the ecological model Daphnia

Author:
Ellen Decaestecker; Pierrick Labbé; Kirsten Ellegaard; Judith E. Allen; Tom J. Little
Source:
Developmental and comparative immunology 2011 v.35 no.10 pp. 1068-1077
ISSN:
0145-305X
Subject:
Daphnia; Drosophila; Pasteuria; asexual reproduction; clones; coevolution; ecological genetics; gene expression; gene expression regulation; genes; genotype; host-pathogen relationships; hosts; immune system; innate immunity; invertebrates; models; nucleotide sequences; parasitism; pathogens; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction
Abstract:
... The last ten years have witnessed increasing interest in host–pathogen interactions involving invertebrate hosts. The invertebrate innate immune system is now relatively well characterised, but in a limited range of genetic model organisms and under a limited number of conditions. Immune systems have been little studied under real-world scenarios of environmental variation and parasitism. Thus, we ...
DOI:
10.1016/j.dci.2011.04.004
PubMed:
21550363
PubMed Central:
PMC3170911

43. Characterization and antiviral function of a cytosolic sensor gene, MDA5, in Japanese flounder, Paralichthys olivaceus

Author:
Maki Ohtani; Jun-ichi Hikima; Hidehiro Kondo; Ikuo Hirono; Tae-Sung Jung; Takashi Aoki
Source:
Developmental and comparative immunology 2011 v.35 no.5 pp. 554-562
ISSN:
0145-305X
Subject:
Hirame rhabdovirus; Infectious pancreatic necrosis virus; Paralichthys olivaceus; RNA; Viral hemorrhagic septicemia virus; cytopathogenicity; cytoplasm; flounder; genes; kidneys; leukocytes; melanoma; nucleotides; receptors; viral load
Abstract:
... Cytosolic pattern recognition receptors such as retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) play an important role in sensing viral RNAs. The receptor encoded by melanoma differentiation-associated gene 5 (MDA5), an RLR, recognizes viral RNA in the cytoplasm and enhances antiviral response in host cells. The full-length MDA5 gene in Japanese flounder, Paralichthys olivaceus was cl ...
DOI:
10.1016/j.dci.2010.12.013

44. Characterization and expression studies of Gaduscidin-1 and Gaduscidin-2; paralogous antimicrobial peptide-like transcripts from Atlantic cod (Gadus morhua)

Author:
Mitchell J. Browne; Charles Y. Feng; Valerie Booth; Matthew L. Rise
Source:
Developmental and comparative immunology 2011 v.35 no.3 pp. 399-408
ISSN:
0145-305X
Subject:
Aeromonas salmonicida; Gadus morhua; antimicrobial peptides; bacterial antigens; blood; brain; cecum; cod (fish); complementary DNA; expressed sequence tags; gene expression; innate immunity; juveniles; kidneys; rapid amplification of cDNA ends; reverse transcription; sequence homology; spleen; tissues
Abstract:
... Piscidins are a family of antimicrobial peptides (AMPs) from fish that constitute an important component of their innate immune system. Based on previously generated Atlantic cod (Gadus morhua) expressed sequence tags (ESTs), we identified sequences representing two paralogous AMP-like transcripts. These Atlantic cod paralogues were termed gaduscidins (GAD-1 and GAD-2), derived from the genus name ...
DOI:
10.1016/j.dci.2010.11.010

45. Characterization and gene expression of transcription factors, PU.1 and C/EBPα driving transcription from the tumor necrosis factor α promoter in Japanese flounder, Paralichthys olivaceus

Author:
Jun-ichi Hikima; Maki Ohtani; Hidehiro Kondo; Ikuo Hirono; Tae-Sung Jung; Takashi Aoki
Source:
Developmental and comparative immunology 2011 v.35 no.3 pp. 304-313
ISSN:
0145-305X
Subject:
Edwardsiella tarda; Paralichthys olivaceus; Streptococcus iniae; bacterial infections; flounder; gene expression; genes; inflammation; messenger RNA; tissues; transcription factors; tumor necrosis factor-alpha
Abstract:
... Both PU.1 and C/EBPα transcription factors play important roles in myeloid development and inflammatory response. These transcripts were cloned from the Japanese flounder (Paralichthys olivaceus) and were highly conserved with those of other vertebrates. PU.1 mRNA was mainly expressed in lymphoid tissues while C/EBPα mRNA was widely expressed in all tissues examined. Higher levels of PU.1 mRNA wer ...
DOI:
10.1016/j.dci.2010.10.003

46. Characterization and immune function of two intracellular sensors, HmTLR1 and HmNLR, in the injured CNS of an invertebrate

Author:
Virginie Cuvillier-Hot; Céline Boidin-Wichlacz; Christian Slomianny; Michel Salzet; Aurélie Tasiemski
Source:
Developmental and comparative immunology 2011 v.35 no.2 pp. 214-226
ISSN:
0145-305X
Subject:
Toll-like receptor 3; immune response; invertebrates; mammals; nerve tissue; neurites
Abstract:
... Unlike mammals, the CNS of the medicinal leech can regenerate damaged neurites, thus restoring neural functions. Our group recently demonstrated that the injured leech nerve cord is able to mount an immune response, which promotes the regenerative processes. This defense mechanism is microorganism-specific, suggesting that the leech CNS is able to discriminate among microbial components. We report ...
DOI:
10.1016/j.dci.2010.09.011

47. Characterization of a TnMAVS protein from Tetraodon nigroviridis

Author:
Zhiming Xiang; Lin Qi; Weijian Chen; Chuanfu Dong; Zhaoyu Liu; Dong Liu; Mengli Huang; Wei Li; Gan Yang; Shaoping Weng; Jianguo He
Source:
Developmental and comparative immunology 2011 v.35 no.11 pp. 1103-1115
ISSN:
0145-305X
Subject:
Tetraodon nigroviridis; caspases; eukaryotic cells; fish; fluorescence; gene overexpression; humans; immune response; liver; luciferase; mitochondria; muscles; signal transduction; spleen; transcription factor NF-kappa B
Abstract:
... A growing family of cellular proteins encoding for caspase activation and the recruitment domain (CARD) plays a crucial role in immunity by sensing viral infections and signaling antiviral immune defenses. We obtained a MAVS-like protein (named TnMAVS) from Tetradon nigroviridis, which contains a CARD domain, a pro-rich domain, and a TM domain similar to human MAVS. A fluorescence assay showed tha ...
DOI:
10.1016/j.dci.2011.03.029

48. Characterization of ovine Toll-like receptor 9 protein coding region, comparative analysis, detection of mutations and maedi visna infection

Author:
Ivan Mikula Jr.; Ivan Mikula Sr.
Source:
Developmental and comparative immunology 2011 v.35 no.2 pp. 182-192
ISSN:
0145-305X
Subject:
Toll-like receptor 9; antigens; genes; humans; innate immunity; mutation; sheep
Abstract:
... One of the major roles of innate immunity system is the recognition and the determination of the nature of the antigen. This ability is encompassed by specific receptors as Toll-like receptors (TLRs). TLR9 recognizes bacterial and viral CpG motifs, while their potent immunostimulation effect seems to be promising for lentiviral therapies. Recent studies, however, show the presence of a big polymor ...
DOI:
10.1016/j.dci.2010.09.008

49. Characterization of the novel antibacterial peptide Leucrocin from crocodile (Crocodylus siamensis) white blood cell extracts

Author:
Supawadee Pata; Nualyai Yaraksa; Sakda Daduang; Yosapong Temsiripong; Jisnuson Svasti; Tomohiro Araki; Sompong Thammasirirak
Source:
Developmental and comparative immunology 2011 v.35 no.5 pp. 545-553
ISSN:
0145-305X
Subject:
Crocodylus siamensis; Salmonella Typhi; Staphylococcus epidermidis; Vibrio cholerae; amino acid sequences; antibacterial properties; antimicrobial peptides; beta-galactosidase; cell membranes; crocodiles; electron microscopy; erythrocytes; extracts; fluorescence; humans; leukocytes; molecular weight; reversed-phase high performance liquid chromatography; toxicity
Abstract:
... Four novel antibacterial peptides, Leucrocin I–IV from Siamese crocodile white blood cell extracts were purified by reverse phase high performance liquid chromatography (RP-HPLC). Leucrocins exhibit strong antibacterial activity towards Staphylococcus epidermidis, Salmonella typhi and Vibrio cholerae. The peptides were 7–10 residues in length with different primary structure. The amino acid sequen ...
DOI:
10.1016/j.dci.2010.12.011

50. Chemokines in teleost fish species

Author:
Alí Alejo; Carolina Tafalla
Source:
Developmental and comparative immunology 2011 v.35 no.12 pp. 1215-1222
ISSN:
0145-305X
Subject:
Oncorhynchus mykiss; chemoattractants; chemokines; cysteine; fish; genes; homeostasis; immune response; innate immunity; invertebrates; mammals
Abstract:
... Chemokines are chemoattractant cytokines defined by the presence of four conserved cysteine residues which in mammals can be divided into four subfamilies depending on the arrangement of the first two conserved cysteines in their sequence: CXC (α), CC (β), C and CX₃C classes. Evolutionarily, fish can be considered as an intermediate step between species which possess only innate immunity (inverteb ...
DOI:
10.1016/j.dci.2011.03.011