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You searched for: Journal name Electrophoresis Remove constraint Journal name: Electrophoresis Publication year rev 7980-2020 Remove constraint Publication year rev: 7980-2020 Source 2020 v.41 no.7-8 Remove constraint Source: 2020 v.41 no.7-8
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1. CE determination of the thermodynamic pKa values and limiting ionic mobilities of 14 low molecular mass UV absorbing ampholytes for accurate characterization of the pH gradient in carrier ampholytes‐based IEF and its numeric simulation

Author:
Martin Ansorge; Bohuslav Gaš; Milan Boublík; Michal Malý; Jana Šteflová; Vlastimil Hruška; Gyula Vigh
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 514-522
ISSN:
0173-0835
Subject:
computer software; electrophoresis; ionic strength; mathematical models; moieties; molecular weight; pH; thermodynamics
Abstract:
... Fourteen low molecular mass UV absorbing ampholytes containing 1 or 2 weakly acidic and 1 or 2 weakly basic functional groups that best satisfy Rilbe's requirement for being good carrier ampholytes (ΔpKₐ = pKaₘₒₙₒₐₙᵢₒₙ ‒ pKaₘₒₙₒcₐₜᵢₒₙ < 2) were selected from a large group of commercially readily available ampholytes in a computational study using two software packages (ChemSketch and SPARC). Their ...
DOI:
10.1002/elps.201900381

2. Capillary electrophoresis–based immunoassay and aptamer assay: A review

Author:
Hana Nevídalová; Lenka Michalcová; Zdeněk Glatz
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 414-433
ISSN:
0173-0835
Subject:
antibodies; chemiluminescence; electrochemistry; electrophoresis; fluorescence; immunoassays; microchip technology; oligonucleotides; prices; proteins; thermal stability
Abstract:
... Over the last two decades, the group of techniques called affinity probe CE has been widely used for the detection and the determination of several types of biomolecules with high sensitivity. These techniques combine the low sample consumption and high separation power of CE with the selectivity of the probe to the target molecule. The assays can be defined according to the type of probe used: CE ...
DOI:
10.1002/elps.201900426

3. Comprehensive model of electromigrative transport in microfluidic paper based analytical devices

Author:
Federico Schaumburg; Pablo A. Kler; Claudio L. A. Berli
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 598-606
ISSN:
0173-0835
Subject:
cellulosic fibers; chemical species; differential equation; electrophoresis; geometry; mathematical models; paper; porous media
Abstract:
... A complete mathematical model for electromigration in paper‐based analytical devices is derived, based on differential equations describing the motion of fluids by pressure sources and EOF, the transport of charged chemical species, and the electric potential distribution. The porous medium created by the cellulose fibers is considered like a network of tortuous capillaries and represented by macr ...
DOI:
10.1002/elps.201900353

4. Contemporary chiral simulators for capillary zone electrophoresis

Author:
Jitka Caslavska; Wolfgang Thormann
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 502-513
ISSN:
0173-0835
Subject:
absorbance; capillary zone electrophoresis; enantiomers; equations
Abstract:
... For separation of enantiomers in presence of a chiral selector, data obtained with the 1D dynamic simulators SIMUL5complex and GENTRANS are compared to data predicted by PeakMaster 6, a recently released generalized model of the linear theory of electromigration. Four electrophoretic systems with stereoisomers of weak bases were investigated. They deal with the estimation of input data for complex ...
DOI:
10.1002/elps.201900363

5. Determination of binding constants of multiple charged cyclodextrin complexes by ACE using uncorrected and ionic strength corrected actual mobilities of the species involved

Author:
Petra Sázelová; Dušan Koval; Lukáš Severa; Filip Teplý; Gyula Vigh; Václav Kašička
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 523-535
ISSN:
0173-0835
Subject:
cyclodextrins; electrophoresis; enantiomers; ionic strength; iron; ruthenium
Abstract:
... In this study, the apparent binding constants and limiting mobilities of the multiply charged complexes of the Δ− and Λ−enantiomers of Ru(II)‐ and Fe(II)‐polypyridyl associates ([Ru(2,2′‐bipyridine)₃]²⁺, [Ru(1,10‐phenanthroline)₃]²⁺, and [Fe(1,10‐phenanthroline)₃]²⁺) with single‐isomer 2,3‐diacetylated‐6‐sulfated‐cyclodextrins (CDs) (12Ac‐6S‐α‐CD, 14Ac‐7S‐β‐CD, and 16Ac‐8S‐γ‐CD) were determined by ...
DOI:
10.1002/elps.201900352

6. Determination of thermodynamic acidity constants and limiting ionic mobilities of weak electrolytes by capillary electrophoresis using a new free software AnglerFish

Author:
Michal Malý; Milan Boublík; Marijana Pocrnić; Martin Ansorge; Kateřina Lorinčíková; Jana Svobodová; Vlastimil Hruška; Pavel Dubský; Bohuslav Gaš
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 493-501
ISSN:
0173-0835
Subject:
acidity; capillary electrophoresis; computer software; dissociation; electrolytes; ionic strength; ionization; pH; temperature; thermodynamics
Abstract:
... Thermodynamic acidity constants (acid or acid‐base dissociation constants, sometimes called also as ionization constants) and limiting ionic mobilities (both of them at defined temperature, usually 25°C) are the fundamental physicochemical characteristics of a weak electrolyte, that is, weak acid or weak base or ampholyte. We introduce a novel method for determining the data of a weak electrolyte ...
DOI:
10.1002/elps.201900283

7. Elastic instabilities in the electroosmotic flow of non‐Newtonian fluids through T‐shaped microchannels

Author:
Le Song; Liandong Yu; Di Li; Purva P. Jagdale; Xiangchun Xuan
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 588-597
ISSN:
0173-0835
Subject:
electric potential difference; electroosmosis; electrophoresis; hyaluronic acid; lab-on-a-chip technology; phosphates; polyacrylamide; polyethylene glycol; polyvinylpyrrolidone; quantitative analysis; viscoelasticity; xanthan gum
Abstract:
... Electroosmotic flow (EOF) has been widely used to transport fluids and samples in micro‐ and nanofluidic channels for lab‐on‐a‐chip applications. This essentially surface‐driven plug‐like flow is, however, sensitive to both the fluid and wall properties, of which any inhomogeneity may draw disturbances to the flow and even instabilities. Existing studies on EOF instabilities have been focused prim ...
DOI:
10.1002/elps.201900331

8. Electrolysis phenomena in electrophoresis

Author:
Tomáš Novotný; Bohuslav Gaš
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 536-544
ISSN:
0173-0835
Subject:
electrical conductivity; electrodes; electrolysis; electrophoresis; pH
Abstract:
... We present a new theoretical approach for calculating changes in the physico‐chemical properties of BGEs for measurements by CZE due to the electrolysis in electrode vials (vessels). Electrolysis is an inevitable phenomenon in any measurement in CZE. Water electrolysis, which occurs in most measurements, can significantly alter the composition of the BGE in electrode vials and in the separation ca ...
DOI:
10.1002/elps.201900411

9. Electrophoretic ratcheting of spherical particles in well/channel microfluidic devices: Making particles move against the net field

Author:
Hanyang Wang; Hendrick W. de Haan; Gary W. Slater
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 621-629
ISSN:
0173-0835
Subject:
DNA; computer simulation; electrophoresis; pulsed electric fields
Abstract:
... We examine the electrophoresis of spherical particles in microfluidic devices made of alternating wells and narrow channels, including a system previously used to separate DNA molecules. Our computer simulations predict that such systems can be used to separate spherical particles of different sizes that share the same free‐solution mobility. Interestingly, the electrophoretic velocity shows an in ...
DOI:
10.1002/elps.201900299

10. Electro‐kinetically driven route for highly sensitive blood pathology on a paper‐based device

Author:
Naresh Kumar Mani; Sankha Shuvra Das; Sayantan Dawn; Suman Chakraborty
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 615-620
ISSN:
0173-0835
Subject:
blood plasma; color; colorimetry; detection limit; electric field; electrophoresis; glucose; paper; point-of-care systems
Abstract:
... Enhancing the sensitivity of colorimetric detection in paper‐devices is a quintessential step in achieving frugal diagnosis. Here, we demonstrate an effective way of improving the detection sensitivity of paper‐based devices, as mediated by electro‐kinetic mechanisms. By directly employing blood plasma, we investigate the electro‐kinetic clustering of glucose, a neutral molecule in paper devices. ...
DOI:
10.1002/elps.201900356

11. Fabrication of hexagonal boron nitride based 2D nanopore sensor for the assessment of electro‐chemical responsiveness of human serum transferrin protein

Author:
Jugal Saharia; Y. M. Nuwan D. Y. Bandara; Jung Soo Lee; Qingxiao Wang; Moon J. Kim; Min Jun Kim
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 630-637
ISSN:
0173-0835
Subject:
DNA; blood serum; boron nitride; capacitance; dielectric properties; electric potential difference; electrophoresis; humans; nanopores; normal distribution; pH; protein composition; transferrin
Abstract:
... In this work, we present a step‐by‐step workflow for the fabrication of 2D hexagonal boron nitride (h‐BN) nanopores which are then used to sense holo‐human serum transferrin (hSTf) protein at pH ∼8 under applied voltages ranging from +100 mV to +800 mV. 2D nanopores are often used for DNA, however, there is a great void in the literature for single‐molecule protein sensing and this, to the best of ...
DOI:
10.1002/elps.201900336

12. Mass transfer of a neutral solute in polyelectrolyte grafted soft nanochannel with porous wall

Author:
Debashis Roy; Saikat Bhattacharjee; Sirshendu De
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 578-587
ISSN:
0173-0835
Subject:
electrolytes; electroosmosis; electrophoresis; equations; mass transfer; permeability; solutes
Abstract:
... A soft nanochannel involves a soft interface that contains a polyelectrolyte layer (PEL) sandwiched between a rigid surface and a bulk electrolyte solution. Mass transfer of a neutral solute in a combined electroosmotic and pressure driven flow through a polyelectrolyte grafted charged nanochannel with porous wall is presented in this work. Assuming the PEL as fixed charged layer and PEL‐electroly ...
DOI:
10.1002/elps.201900282

13. Nonaqueous electrophoresis on microchips: A review

Author:
Lucas M. Duarte; Roger C. Moreira; Wendell K. T. Coltro
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 434-448
ISSN:
0173-0835
Subject:
electrolytes; electrophoresis; microchip technology; solubility; solvents
Abstract:
... The use of organic solvents as electrolytic medium in electrophoresis has become an important alternative for the analysis of compounds that exhibit low or no solubility in water. In recent years, nonaqueous electrophoresis has been extensively explored in conventional capillary systems for different applications. On the other hand, this separation strategy is still not as popular as free solution ...
DOI:
10.1002/elps.201900238

14. Theory of electrophoretic focusing on an inverse electromigration dispersion profile

Author:
Petr Gebauer
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 471-480
ISSN:
0173-0835
Subject:
electric current; electric potential difference; electrolytes; electroosmosis; hydrodynamics; isoelectric focusing
Abstract:
... Electrophoretic focusing on an inverse electromigration dispersion (EMD) profile is based on a principle different from those of other electrophoretic separation methods. It has already proved its applicability in analytical practice by offering competitive separation performance and sensitivity and specific selectivity. It can be classified as an intermediate between field‐driven and equilibrium ...
DOI:
10.1002/elps.201900229

15. The effect of the finite size of ions and Debye layer overspill on the screened Coulomb interactions between charged flat plates

Author:
Arghyadeep Paul; Siddhartha Mukherjee; Jayabrata Dhar; Sandip Ghosal; Suman Chakraborty
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 607-614
ISSN:
0173-0835
Subject:
electrophoresis; ions; logit analysis; nanomaterials
Abstract:
... Screened repulsion between uniformly charged plates with an intervening electrolyte is analyzed for strongly overlapped electrical double layers (EDL), accounting for the steric effect of ions and their expulsion from EDL edges into the surrounding solution. As a generalization of a study by Philipse et al. which does not account for these effects, an analytical expression is derived for the repul ...
DOI:
10.1002/elps.201900318

16. Effect of surface conduction–induced electromigration on current monitoring method for electroosmotic flow measurement

Author:
Mohammad Babar; Kaushlendra Dubey; Supreet Singh Bahga
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 570-577
ISSN:
0173-0835
Subject:
electrolytes; electroosmosis; electrophoresis; glass; monitoring; organ-on-a-chip
Abstract:
... Current monitoring method for measurement of EOF in microchannels involves measurement of time‐varying current while an electrolyte displaces another electrolyte having different conductivity due to EOF. The basic premise of the current monitoring method is that an axial gradient in conductivity of a binary electrolyte in a microchannel advects only due to EOF. In the current work, using theory an ...
DOI:
10.1002/elps.201900308

17. Enhancement in MS‐based peptide detection by microfluidic free‐flow zone electrophoresis

Author:
Tristan F. Kinde; Natalie Hess; Debashis Dutta
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 545-553
ISSN:
0173-0835
Subject:
DNA; angiotensin II; blood serum; detection limit; electric field; electrodes; electrophoresis; electrospray ionization mass spectrometry; fractionation; ionization; solvents
Abstract:
... Matrix components are known to significantly alter the ionization of a target analyte in ESI‐based measurements particularly when working with complex biological samples. This issue however may be alleviated by extracting the analyte of interest from the original sample into a relatively simple matrix compatible with ESI mass‐spectrometric analysis. In this article, we report a microfluidic device ...
DOI:
10.1002/elps.201900321

18. Ionic current in nanochannels grafted with pH‐responsive polyelectrolyte brushes modeled using augmented strong stretching theory

Author:
Harnoor Singh Sachar; Vishal Sankar Sivasankar; Sai Ankit Etha; Guang Chen; Siddhartha Das
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 554-561
ISSN:
0173-0835
Subject:
electrophoresis; hydrogen ions; nanomaterials; pH; salt concentration
Abstract:
... In this paper, we provide a theory to quantify the ionic current (iion) in nanochannels grafted with pH‐responsive polyelectrolyte (PE) brushes. We consider the PE brushes to be modeled by our recently proposed augmented strong stretching theory (SST) model that improves the existing SST models by incorporating the effects of excluded volume interactions and an extended mass action law. Use of suc ...
DOI:
10.1002/elps.201900248

19. Mechanical characterization of vesicles and cells: A review

Author:
Adnan Morshed; Buddini Iroshika Karawdeniya; Y.M. Nuwan D.Y. Bandara; Min Jun Kim; Prashanta Dutta
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 449-470
ISSN:
0173-0835
Subject:
bending strength; capacitance; cell engineering; cholesterol; drug carriers; drugs; electrophoresis; mechanical loads; mechanical stress; pharmaceutical industry; sphingomyelins; viscosity
Abstract:
... Vesicles perform many essential functions in all living organisms. They respond like a transducer to mechanical stress in converting the applied force into mechanical and biological responses. At the same time, both biochemical and biophysical signals influence the vesicular response in bearing mechanical loads. In recent years, liposomes, artificial lipid vesicles, have gained substantial attenti ...
DOI:
10.1002/elps.201900362

20. USB powered microfluidic paper‐based analytical devices

Author:
Federico Schaumburg; Pablo A. Kler; Cody S. Carrell; Claudio L. A. Berli; Charles S. Henry
Source:
Electrophoresis 2020 v.41 no.7-8 pp. 562-569
ISSN:
0173-0835
Subject:
capillarity; electrolytes; electronic equipment; electrophoresis; human resources; point-of-care systems; prediction
Abstract:
... Microfluidic paper‐based analytical devices (μPADs) allow user‐friendly and portable chemical determinations, although they provide limited applicability due to insufficient sensitivity. Several approaches have been proposed to address poor sensitivity in μPADs, but they frequently require bulky equipment for power and/or read‐outs. Universal serial buses (USB) are an attractive alternative to les ...
DOI:
10.1002/elps.201900273