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- Author:
- Zilberstein, Gleb; Zilberstein, Roman; Zilberstein, Svetlana; Fau, Guillaume; D'Amato, Alfonsina; Righetti, Pier Giorgio
- Source:
- Electrophoresis 2019 v.40 no.23-24 pp. 3050-3056
- ISSN:
- 0173-0835
- Subject:
- Lactobacillus; Streptococcus uberis; animal diseases; bacteria; cations; cultural heritage; electrophoresis; ethylene-vinyl acetate; mass spectrometry; resins; saliva; sexually transmitted diseases; France
- Abstract:
- ... The original manuscript of Casanova's Memoirs is stored at the Bibliothèque Nationale de France in Paris. We have gained access to it and explored the surfaces of chapters one and two (via the ethylene vinyl acetate [EVA] film technology, i.e., of diskettes of ethylene vinyl acetate with embedded strong cation and anion exchangers and C8 resins) in search of potential diseases of the author, espec ...
- DOI:
- 10.1002/elps.201800505
-
http://dx.doi.org/10.1002/elps.201800505
- Author:
- Boulanger, Madeleine; Delvaux, Cédric; Quinton, Loïc; Joris, Bernard; De Pauw, Edwin; Far, Johann
- Source:
- Electrophoresis 2019 v.40 no.20 pp. 2672-2682
- ISSN:
- 0173-0835
- Subject:
- Bacillus licheniformis; N-acetylglucosamine; acetylation; bacteria; cell growth; cell walls; electron transfer; electrophoresis; glycopeptides; hydrophilicity; lysozyme; moieties; peptidoglycans; polymers; reversed-phase liquid chromatography; screening
- Abstract:
- ... Peptidoglycan or murein is an essential polymer found in bacterial cell wall. It is a dynamic structure that is continuously remodeled or modified during bacterial cell growth or in presence of cell wall stresses. These modifications are still poorly understood mainly due to the peptidoglycan, which is rather non‐soluble, and the difficulties to separate the hydrophilic glycopeptides (muropeptides ...
- DOI:
- 10.1002/elps.201900147
-
http://dx.doi.org/10.1002/elps.201900147
- Author:
- Wang, Chao; Zhang, Qiaoli; Wang, Yang; Tang, Xudong; An, Yanan; Li, Shulin; Xu, Hongyue; Li, Yan; Luan, Wenjing; Wang, Xuefei; Liu, Mingyuan; Yu, Lu
- Source:
- Electrophoresis 2019 v.40 no.20 pp. 2736-2746
- ISSN:
- 0173-0835
- Subject:
- Mycobacterium tuberculosis; anti-infective agents; antigens; bacteria; binding proteins; biofilm; catalytic activity; cell walls; drug resistance; electrophoresis; gene ontology; liquid chromatography; mass spectrometry; metabolism; pathogenicity; phenotype; plankton; proteomics; tuberculosis; vaccines
- Abstract:
- ... Tuberculosis is highly persistent and displays phenotypic resistance to high concentrations of antimicrobials. Recent reports exhibited that Mycobacterium tuberculosis biofilm was implicated to its pathogenicity and drug resistance. In this study, there were 47 kinds of differential proteins in the biofilm of M. tuberculosis H37Rv cells compared with the planktonic bacteria, and 37 proteins were n ...
- DOI:
- 10.1002/elps.201900030
-
http://dx.doi.org/10.1002/elps.201900030
- Author:
- Wang, Wei; Bai, Ruiguang; Zhang, Huimin; Cai, Xiaoyu
- Source:
- Electrophoresis 2019 v.40 no.20 pp. 2665-2671
- ISSN:
- 0173-0835
- Subject:
- Corynebacterium glutamicum; bacteria; carbon; derivatization; electrophoresis; glucose; lysine; metabolism; metabolites; micellar electrokinetic capillary chromatography; microbial fuel cells; nitrogen; sulfur; valine
- Abstract:
- ... Corynebacterium glutamicum (C. glutamicum) is a well‐known workhorse for the industrial production of amino acids. Different carbon, nitrogen, and sulfur source may force the bacterium to produce specific metabolites. In this work, a method of high‐speed MEKC with LIF detection was developed to rapidly analyze the amino acid metabolites released by C. glutamicum, which is fed with different cultur ...
- DOI:
- 10.1002/elps.201900010
-
http://dx.doi.org/10.1002/elps.201900010
- Author:
- Pethig, Ronald
- Source:
- Electrophoresis 2019 v.40 no.18-19 pp. 2575-2583
- ISSN:
- 0173-0835
- Subject:
- DNA; bacteria; dielectrophoresis; electric impedance; electrophoresis; proteins; virion
- Abstract:
- ... Dielectrophoresis (DEP) studies have progressed from the microscopic scale of cells and bacteria, through the mesoscale of virions to the molecular scale of DNA and proteins. The Clausius‐Mossotti function, based on macroscopic electrostatics, is invariably employed in the analyses of all these studies. The limitations of this practice are explored, with the conclusion that it should be abandoned ...
- DOI:
- 10.1002/elps.201900057
-
http://dx.doi.org/10.1002/elps.201900057
- Author:
- Chen, Na; Meng, Xinyu; Ding, Pan; Su, Yuanyuan; Wang, Houyu; He, Yao
- Source:
- Electrophoresis 2019 v.40 no.16-17 pp. 2172-2179
- ISSN:
- 0173-0835
- Subject:
- Raman spectroscopy; bacteria; biomimetics; detection limit; electrophoresis; fouling; hydrogen peroxide; mercury; municipal wastewater; nanosilver; nitric acid; polyphenols; reducing agents; silicon; silver; tea; vibration
- Abstract:
- ... The fouling and stability are two most critical limiting factors for practical applications of surface‐enhanced Raman scattering (SERS)‐based microfluidic electrophoresis device. Herein, we present a novel biomimetic nanoengineering strategy to achieve a SERS substrate featuring antifouling ability, good stability, and reliable quantitative capability. Typically, by employing tea polyphenol as the ...
- DOI:
- 10.1002/elps.201800538
-
http://dx.doi.org/10.1002/elps.201800538
- Author:
- Jiang, Yu; Wu, Jayne
- Source:
- Electrophoresis 2019 v.40 no.16-17 pp. 2084-2097
- ISSN:
- 0173-0835
- Subject:
- agricultural industry; bacteria; biocompatibility; bioprocessing; biosensors; chitosan; electrophoresis; environmental monitoring; glucose; moieties; nanocomposites; nanomaterials; permeability; proteins; strength (mechanics)
- Abstract:
- ... Recent years have witnessed ever expanding use of biosensors in the fields of environmental monitoring, homeland security, pharmaceutical, food and bioprocessing, and agricultural industries. To produce effective and reliable biosensors, good quality immobilization of biological recognition elements is critical. Chitosan and its nanocomposites emerge as an excellent immobilization matrix on biosen ...
- DOI:
- 10.1002/elps.201900066
- CHORUS:
- 10.1002/elps.201900066
-
http://dx.doi.org/10.1002/elps.201900066
- Author:
- Hanson, Cynthia; Barney, Jacob T.; Bishop, Morgan M.; Vargis, Elizabeth
- Source:
- Electrophoresis 2019 v.40 no.10 pp. 1446-1456
- ISSN:
- 0173-0835
- Subject:
- Raman spectroscopy; agar; bacteria; dielectrophoresis; electrodes; electrophoresis; fouling; heat; polystyrenes
- Abstract:
- ... The traditional bacterial identification method of growing colonies on agar plates can take several days to weeks to complete depending on the growth rate of the bacteria. Successfully decreasing this analysis time requires cell isolation followed by identification. One way to decrease analysis time is by combining dielectrophoresis (DEP), a common technique used for cell sorting and isolation, an ...
- DOI:
- 10.1002/elps.201800389
-
http://dx.doi.org/10.1002/elps.201800389
- Author:
- Wang, Yanjuan; Wang, Junsheng; Wu, Xudong; Jiang, Zong; Wang, Wei
- Source:
- Electrophoresis 2019 v.40 no.6 pp. 969-978
- ISSN:
- 0173-0835
- Subject:
- Closterium; bacteria; ballast water; dielectrophoresis; electric field; electrodes; electrophoresis; microalgae; microparticles; microplastics; organ-on-a-chip
- Abstract:
- ... The composition of the ship's ballast water is complex and contains a large number of microalgae cells, bacteria, microplastics, and other microparticles. To increase the accuracy and efficiency of detection of the microalgae cells in ballast water, a new microfluidic chip for continuous separation of microalgae cells based on alternating current dielectrophoresis was proposed. In this microfluidi ...
- DOI:
- 10.1002/elps.201800302
-
http://dx.doi.org/10.1002/elps.201800302
- Author:
- Chen, Kang; Wang, Jie; Li, Shiyan; Wang, Yang; Zhang, Yiqi; Shen, Qing
- Source:
- Electrophoresis 2019 v.40 no.4 pp. 555-562
- ISSN:
- 0173-0835
- Subject:
- Mycoplasma; Spirochaetes; aquaculture; bacteria; electrophoresis; labor; methanol; microparticles; pH; protocols; quarantine; solid phase extraction; solvents; sorbents; tandem mass spectrometry; tetracycline; ultra-performance liquid chromatography
- Abstract:
- ... Tetracyclines abuse has frequently occurred in aquaculture against bacteria, rickettsiae, spirochetes, and mycoplasmas. In this study, a high‐throughput sample preparation method was developed using 96‐well plate solid‐phase extraction (p‐SPE) and the extract was analyzed by ultra‐performance liquid chromatography tandem mass spectrometry (UPLC‐MS/MS). The experimental conditions were optimized su ...
- DOI:
- 10.1002/elps.201800473
-
http://dx.doi.org/10.1002/elps.201800473
- Author:
- Abdullah, Amjed; Dastider, Shibajyoti Ghosh; Jasim, Ibrahem; Shen, Zhenyu; Yuksek, Nuh; Zhang, Shuping; Dweik, Majed; Almasri, Mahmoud
- Source:
- Electrophoresis 2019 v.40 no.4 pp. 508-520
- ISSN:
- 0173-0835
- Subject:
- Escherichia coli O157; Salmonella Typhimurium; antibodies; bacteria; biosensors; chicken meat; dielectrophoresis; electrodes; electrophoresis; glass; pathogens; raw chicken meat
- Abstract:
- ... A MEMS‐based impedance biosensor was designed, fabricated, and tested to effectively detect the presence of bacterial cells including E. coli O157:H7 and Salmonella typhimurium in raw chicken products using detection region made of multiple interdigitated electrode arrays. A positive dielectrophoresis based focusing electrode was used in order to focus and concentrate the bacterial cells at the ce ...
- DOI:
- 10.1002/elps.201800405
-
http://dx.doi.org/10.1002/elps.201800405