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1. A CE‐LIF method based on long wavelength fluorescence labeling for the analysis of thiols in human urine

Author:
Duan, Ya‐Jun; Zhang, Li‐Yun; Guo, Xiao‐Feng; Wang, Hong
Source:
Electrophoresis 2016 v.37 no.14 pp. 2097-2105
ISSN:
0173-0835
Subject:
acetonitrile; acetylcysteine; borates; cysteine; derivatization; detection limit; electrophoresis; fluorescence; fluorescent dyes; glutathione; homocysteine; humans; pH; penicillamine; phosphates; thiols; urine; wavelengths
Abstract:
... A rapid and robust CE method using a long wavelength fluorescent reagent 1,7‐dimethyl‐3,5‐distyryl‐8‐phenyl‐(2‐maleimide)difluoroboradiaza‐s‐indacene as the labeling reagent has been developed for the simultaneous determination of thiols, including glutathione, cysteine, homocysteine, N‐acetylcysteine, cysteinylglycine, and penicillamine. The derivatization reaction was carried out in 14 mmol/L pH ...
DOI:
10.1002/elps.201600148