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- Zhu, Guijie; Sun, Liangliang; Heidbrink‐Thompson, Jennifer; Kuntumalla, Srilatha; Lin, Hung‐yu; Larkin, Christopher J.; McGivney, James B., IV; Dovichi, Norman J.
- Electrophoresis 2016 v.37 no.4 pp. 616-622
- capillary zone electrophoresis; humans; monoclonal antibodies; pH; proteins; tandem mass spectrometry; therapeutics
- ... We have evaluated CZE‐ESI‐MS/MS for detection of trace amounts of host cell protein impurities in recombinant therapeutics. Compared to previously published procedures, we have optimized the buffer pH used in the formation of a pH junction to increase injection volume. We also prepared a 5‐point calibration curve by spiking 12 standard proteins into a solution of a human mAb. A custom CZE‐MS/MS sy ...
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