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- Liu, Yan‐Ming; Cao, Jun‐Tao; Liu, Ying‐Ying; Zhang, Jing‐Jing; Zhou, Min; Huang, Ke‐Jing; Chen, Yong‐Hong; Ren, Shu‐Wei
- Electrophoresis 2015 v.36 no.19 pp. 2413-2418
- blood serum; capillary electrophoresis; chemiluminescence; detection limit; humans; immunoglobulin E; models; nanogold; oligonucleotides; quantitative analysis
- ... A novel aptamer‐based CE with chemiluminescence (CL) assay was developed for highly sensitive detection of human immunoglobulin E (IgE). The IgE aptamer was conjugated with gold nanoparticles (AuNPs) to form AuNPs‐aptamer that could specifically recognize the IgE to produce an AuNPs‐aptamer‐IgE complex. The mixture of the AuNPs‐aptamer‐IgE complex and the unbounded AuNPs‐aptamer could be effective ...