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You searched for: Journal Environmental microbiology Remove constraint Journal: Environmental microbiology Publication Year 2008 Remove constraint Publication Year: 2008 Source 2008 v.10 no.10 Remove constraint Source: 2008 v.10 no.10
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1. A large gene cluster encoding peptide synthetases and polyketide synthases is involved in production of siderophores and oxidative stress response in the cyanobacterium Anabaena sp. strain PCC 7120

Author:
Jeanjean, Robert; Talla, Emmanuel; Latifi, Amel; Havaux, Michel; Janicki, Annick; Zhang, Cheng-Cai
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2574-2585
ISSN:
1462-2912
Subject:
Anabaena; chromosomes; iron; ligases; multigene family; mutants; oxidative stress; polyketide synthases; secondary metabolites; siderophores; stress response
Abstract:
... Non-ribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs) are necessary for the production of a variety of secondary metabolites, such as siderophores involved in iron acquisition. In response to iron limitation, the cyanobacterium Anabaena sp. strain PCC 7120 synthesizes several siderophores. The chromosome of this organism contains a large gene cluster of 76 kb with 24 open-readi ...
DOI:
10.1111/j.1462-2920.2008.01680.x

2. Assigning Escherichia coli strains to phylogenetic groups: multi-locus sequence typing versus the PCR triplex method

Author:
Gordon, David M.; Clermont, Olivier; Tolley, Heather; Denamur, Erick
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2484-2496
ISSN:
1462-2912
Subject:
DNA; Escherichia coli; algorithms; genes; genotype; life history; niches; nucleotide sequences; phylogeny; polymerase chain reaction
Abstract:
... It is well recognized that Escherichia coli consists of a number of distinct phylo-groups and that strains of the different phylo-groups vary in their ecological niches, life-history characteristics and propensity to cause disease. Consequently, much can be learnt by assigning a strain of E. coli to one of the recognized phylo-groups. A triplex PCR-based method that enables strains of E. coli to b ...
DOI:
10.1111/j.1462-2920.2008.01669.x
PubMed:
18518895

3. Biodiversity of amoebae and amoebae-resisting bacteria in a drinking water treatment plant

Author:
Thomas, Vincent; Loret, Jean-François; Jousset, Michel; Greub, Gilbert
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2728-2745
ISSN:
1462-2912
Subject:
Acanthamoeba castellanii; Legionella; Mycobacterium mucogenicum; Parachlamydia acanthamoebae; activated carbon; bacteria; biodiversity; biofilm; coculture; disinfection; drinking water; filters; filtration; hosts; ozonation; risk reduction; river water; rivers; sand; water treatment
Abstract:
... The complex ecology of free-living amoebae (FLA) and their role in spreading pathogenic microorganisms through water systems have recently raised considerable interest. In this study, we investigated the presence of FLA and amoebae-resisting bacteria (ARB) at various stages of a drinking water plant fed with river water. We isolated various amoebal species from the river and from several points wi ...
DOI:
10.1111/j.1462-2920.2008.01693.x
PubMed:
18637950

4. Burkholderia cenocepacia strains isolated from cystic fibrosis patients are apparently more invasive and more virulent than rhizosphere strains

Author:
Pirone, Luisa; Bragonzi, Alessandra; Farcomeni, Alessio; Paroni, Moira; Auriche, Cristina; Conese, Massimo; Chiarini, Luigi; Dalmastri, Claudia; Bevivino, Annamaria; Ascenzioni, Fiorentina
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2773-2784
ISSN:
1462-2912
Subject:
Burkholderia; DNA; amidase; cystic fibrosis; epithelial cells; genes; lungs; mice; microbial load; models; mortality; patients; rhizosphere; virulence
Abstract:
... Given the widespread presence of Burkholderia cenocepacia in the rhizosphere it is important to determine whether rhizosphere strains are pathogenic for cystic fibrosis patients or not. Eighteen B. cenocepacia strains of rhizosphere and clinical origin were typed by multi-locus sequence typing (MLST) analysis and compared for their ability to invade pulmonary epithelial cells and their virulence i ...
DOI:
10.1111/j.1462-2920.2008.01697.x
PubMed:
18643926

5. Colonization of overlaying water by bacteria from dry river sediments

Author:
Fazi, Stefano; Amalfitano, Stefano; Piccini, Claudia; Zoppini, Annamaria; Puddu, Alberto; Pernthaler, Jakob
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2760-2772
ISSN:
1462-2912
Subject:
Actinobacteria; Brevundimonas; Caulobacter; DNA; alkaline phosphatase; bacteria; community structure; enzyme activity; microbial activity; nucleotide sequences; plankton; predation; ribosomal RNA; rivers; sediments; sequence analysis; Italy; Sardinia
Abstract:
... We studied the diversity, community composition and activity of the primary microbial colonizers of the water above freshly re-wetted sediments from a temporary river. Dried sediments, collected from Mulargia River (Sardinia, Italy), were covered with sterile freshwater in triplicate microcosms, and changes of the planktonic microbial assemblage were monitored over a 48 h period. During the first ...
DOI:
10.1111/j.1462-2920.2008.01695.x
PubMed:
18643927

6. ColRS two-component system prevents lysis of subpopulation of glucose-grown Pseudomonas putida

Author:
Putrinš, Marta; Ilves, Heili; Kivisaar, Maia; Hõrak, Rita
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2886-2893
ISSN:
1462-2912
Subject:
DNA; Pseudomonas putida; bacteria; carbon; cell membranes; flow cytometry; glucose; iodides; membrane permeability; mutants; proteins; Republic of the Congo
Abstract:
... ColRS two-component system is well conserved in pseudomonads, but its exact role has remained obscure. Here, we report that Pseudomonas putida deficient in ColR experiences serious carbon source-specific stress that leads to the lysis of a subpopulation of bacteria growing on solid glucose medium. We observed that on glucose medium colR-deficient bacteria aggregated, produced a Congo Red-binding s ...
DOI:
10.1111/j.1462-2920.2008.01705.x
PubMed:
18657172

7. Comparative analysis of genome fragments of Acidobacteria from deep Mediterranean plankton

Author:
Quaiser, Achim; López-García, Purificación; Zivanovic, Yvan; Henn, Matthew R.; Rodriguez-Valera, Francisco; Moreira, David
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2704-2717
ISSN:
1462-2912
Subject:
Acidobacteria; ecosystems; genomic libraries; microbial communities; open reading frames; operon; plankton; soil; soil sequences
Abstract:
... Acidobacteria constitute a still poorly studied phylum that is well represented in soils. Recent studies suggest that members of this phylum may be also abundant in deep-sea plankton, but their relative abundance and ecological role in this ecosystem are completely unknown. A recent screening of three metagenomic deep-sea libraries of bathypelagic plankton from the South Atlantic (1000 m depth), t ...
DOI:
10.1111/j.1462-2920.2008.01691.x
PubMed:
18627413

8. Development and evaluation of a quantitative real-time PCR assay for the detection of saltwater Bacteriovorax

Author:
Zheng, Guili; Wang, Cynthia; Williams, Henry N.; Pineiro, Silvia A.
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2515-2526
ISSN:
1462-2912
Subject:
Bacteriovorax; Gram-negative bacteria; detection limit; genes; nucleotide sequences; paper; polymerase chain reaction; population distribution; population dynamics; quantitative analysis; reproduction; ribosomal DNA; ribosomal RNA; saline water
Abstract:
... Bdellovibrio-and-like-organisms (BALOs) are small, Gram-negative predatory bacteria with the ability to prey on a wide variety of Gram-negative bacteria, and which may have a significant ecological role. Detection and quantification of BALOs by culture-dependent methods are complicated, as their reproduction is dependent upon the use of appropriate prey. For this reason, a sensitive and specific m ...
DOI:
10.1111/j.1462-2920.2008.01676.x
PubMed:
18631362

9. Differential pathogenicity and genetic diversity among Pectobacterium carotovorum ssp. carotovorum isolates from monocot and dicot hosts support early genomic divergence within this taxon

Author:
Yishay, Moran; Burdman, Saul; Valverde, Angel; Luzzatto, Tal; Ophir, Ron; Yedidia, Iris
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2746-2759
ISSN:
1462-2912
Subject:
Brassica oleracea; Geographical Locations; Ornithogalum; Pectobacterium carotovorum subsp. carotovorum; Solanum tuberosum; Zantedeschia aethiopica; amplified fragment length polymorphism; genetic variation; hosts; nucleotide sequences; ornamental plants; pathogens; ribosomal RNA; virulence
Abstract:
... The capability of Pectobacterium carotovorum isolates to infect monocotyledonous plants has been previously reported; however, no full consideration was given to characterize the association between such isolates and their monocot hosts. To assess differences in aggressiveness among P. carotovorum ssp. carotovorum isolates originating from monocotyledonous or dicotyledonous plants, we used as mode ...
DOI:
10.1111/j.1462-2920.2008.01694.x
PubMed:
18681897

10. Diversification of unicellular eukaryotes: cryptomonad colonizations of marine and fresh waters inferred from revised 18S rRNA phylogeny

Author:
Shalchian-Tabrizi, Kamran; Bråte, Jon; Logares, Ramiro; Klaveness, Dag; Berney, Cédric; Jakobsen, Kjetill S.
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2635-2644
ISSN:
1462-2912
Subject:
Cryptomonas; eukaryotic cells; freshwater; habitats; history; nucleotide sequences; phylogeny; ribosomal RNA
Abstract:
... The cryptomonads is a well-defined lineage of unicellular eukaryotes, composed of several marine and freshwater groups. However, the evolutionary relationships among these groups are unclear due to conflicting inferences between morphological and molecular phylogenies. Here, we have inferred the evolutionary relationships among marine and freshwater species in order to better understand the import ...
DOI:
10.1111/j.1462-2920.2008.01685.x
PubMed:
18643928

11. Enriching plant microbiota for a metagenomic library construction

Author:
Wang, Hao-Xin; Geng, Zhao-Liang; Zeng, Ying; Shen, Yue-Mao
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2684-2691
ISSN:
1462-2912
Subject:
Actinobacteria; Proteobacteria; biomass; drugs; genes; genomic libraries; host plants; microorganisms; nucleotide sequences; oceans; plant tissues; ribosomal DNA; ribotypes; sequence analysis; sodium chloride; sodium dodecyl sulfate; soil
Abstract:
... Plant microbiota (the microorganisms that live in any associations with plant tissues) represents a rather unexplored area of metagenomic research compared with soils and oceans. Constructing a metagenomic library for plant microbiota is technically challenging. Using all the biomass without pre-enrichment could lead to vast proportions of the host plant DNA in the metagenomic library, doubtless o ...
DOI:
10.1111/j.1462-2920.2008.01689.x
PubMed:
18631363

12. Enrichment of a dioxin-dehalogenating Dehalococcoides species in two-liquid phase cultures

Author:
Bunge, Michael; Wagner, Anke; Fischer, Marco; Andreesen, Jan R.; Lechner, Ute
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2670-2683
ISSN:
1462-2912
Subject:
Dehalococcoides; bacteria; dechlorination; electrons; enrichment culture; genes; hexadecane; hydrogen; isolation techniques; methanogens; microscopy; organic acids and salts; polymerase chain reaction; restriction fragment length polymorphism; ribosomal RNA; vancomycin
Abstract:
... Enrichment cultures capable of reductively dechlorinating 1,2,4-trichlorodibenzo-p-dioxin (1,2,4-TrCDD) were shown to dechlorinate 1,2,3-trichlorobenzene (1,2,3-TrCB) to 1,3-dichlorobenzene. To test if this activity can be used to enrich for dioxin-dechlorinating bacteria, a two-liquid phase cultivation with 200 mM 1,2,3-TrCB dissolved in hexadecane was established. During the dechlorination of 1, ...
DOI:
10.1111/j.1462-2920.2008.01688.x
PubMed:
18627411

13. Environmental distribution and population biology of Candidatus Accumulibacter, a primary agent of biological phosphorus removal

Author:
Peterson, S. Brook; Warnecke, Falk; Madejska, Julita; McMahon, Katherine D.; Hugenholtz, Philip
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2692-2703
ISSN:
1462-2912
Subject:
activated sludge; environmental impact; estuarine sediments; freshwater; genes; habitats; metagenomics; phosphates; phosphorus; phylogeny; population distribution; wastewater treatment
Abstract:
... Members of the uncultured bacterial genus Candidatus Accumulibacter are capable of intracellular accumulation of inorganic phosphate in activated sludge wastewater treatment plants (WWTPs) performing enhanced biological phosphorus removal, but were also recently shown to inhabit freshwater and estuarine sediments. Additionally, metagenomic sequencing of two bioreactor cultures enriched in Candidat ...
DOI:
10.1111/j.1462-2920.2008.01690.x
PubMed:
18643843
PubMed Central:
PMC2561248

14. Epibiotic bacteria associated with the recently discovered Yeti crab, Kiwa hirsuta

Author:
Goffredi, Shana K.; Jones, William J.; Erhlich, Hermann; Springer, Armin; Vrijenhoek, Robert C.
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2623-2634
ISSN:
1462-2912
Subject:
Decapoda; bacteria; biogeochemical cycles; crabs; electron microscopy; enzymes; fluorescence microscopy; legs; oxidation; ribotypes; sulfates; sulfur; tricarboxylic acid cycle
Abstract:
... The Yeti crab, Kiwa hirsuta Macpherson et al., is the single known species in a recently discovered crab family Kiwaidae (Decapoda: Galatheoidea) from deep-sea hydrothermal vents. Its chelipeds, walking legs and the ventral surface of its cephalothorax are covered with dense setae that, in turn, are covered with clusters of filamentous bacteria, making the crab appear extraordinarily 'hairy'. Elec ...
DOI:
10.1111/j.1462-2920.2008.01684.x
PubMed:
18564185

15. Fingerprinting the genetic diversity of the biotin carboxylase gene (accC) in aquatic ecosystems as a potential marker for studies of carbon dioxide assimilation in the dark

Author:
Auguet, Jean-Christophe; Borrego, Carles M.; Bañeras, Lluís; Casamayor, Emilio O.
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2527-2536
ISSN:
1462-2912
Subject:
Crenarchaeota; DNA fingerprinting; DNA primers; bacteria; biogeochemistry; carbon; carbon dioxide; denaturing gradient gel electrophoresis; ecosystems; enzymes; genes; genetic variation; lakes; mountains; nucleotide sequences; polymerase chain reaction; ribosomal RNA; sequence analysis; surveys
Abstract:
... We designed and tested a set of specific primers for specific PCR amplification of the biotin carboxylase subunit gene (accC) of the Acetyl CoA carboxylase (ACCase) enzyme. The primer set yielded a PCR product of c. 460 bp that was suitable for denaturing gradient gel electrophoresis (DGGE) fingerprinting followed by direct sequencing of excised DGGE bands and sequence analysis. Optimization of PC ...
DOI:
10.1111/j.1462-2920.2008.01677.x
PubMed:
18557770

16. High beta diversity of bacteria in the shallow terrestrial subsurface

Author:
Wang, Jianjun; Wu, Yucheng; Jiang, Hongchen; Li, Chunhai; Dong, Hailiang; Wu, Qinglong; Soininen, Janne; Shen, Ji
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2537-2549
ISSN:
1462-2912
Subject:
bacteria; bacterial communities; community structure; denaturing gradient gel electrophoresis; environmental factors; history; sediments
Abstract:
... While there have been a vast number of studies on bacterial alpha diversity in the shallow terrestrial subsurface, beta diversity - how the bacterial community composition changes with spatial distance - has received surprisingly limited attention. Here, bacterial beta diversity and its controlling factors are investigated by denaturing gradient gel electrophoresis and cloning of samples from a 70 ...
DOI:
10.1111/j.1462-2920.2008.01678.x

17. Host-specificity of environmentally transmitted Mycoplasma-like isopod symbionts

Author:
Fraune, Sebastian; Zimmer, Martin
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2497-2504
ISSN:
1462-2912
Subject:
diet; foods; survival rate; longevity; humans; Isopoda; hosts; symbionts; midgut; phylogeny; host specificity; habitats; pathogens
Abstract:
... Mycoplasms are known as pathogens of economic and medical interest in plants, animals and man. Here, we show a positive correlation between the presence of Mycoplasma-like symbionts in their isopod hosts and survivorship on low-quality food. Most isopods that survived feeding on a cellulose-based low-quality diet for 90 days harboured 'Candidatus Hepatoplasma' in their midgut glands, while those t ...
DOI:
10.1111/j.1462-2920.2008.01672.x

18. How natural infection by Nosema ceranae causes honeybee colony collapse

Author:
Higes, Mariano; Martín-Hernández, Raquel; Botías, Cristina; Bailón, Encarna Garrido; González-Porto, Amelia V.; Barrios, Laura; del Nozal, M. Jesús; Bernal, José L.; Jiménez, Juan J.; Palencia, Pilar García; Meana, Aránzazu
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2659-2669
ISSN:
1462-2912
Subject:
etiology; Apis mellifera; winter; Nosema ceranae; antibiotics; honey bees; Apis cerana; disease control; spring; death; brood rearing; pollen; honey; honey bee colonies
Abstract:
... In recent years, honeybees (Apis mellifera) have been strangely disappearing from their hives, and strong colonies have suddenly become weak and died. The precise aetiology underlying the disappearance of the bees remains a mystery. However, during the same period, Nosema ceranae, a microsporidium of the Asian bee Apis cerana, seems to have colonized A. mellifera, and it's now frequently detected ...
DOI:
10.1111/j.1462-2920.2008.01687.x
PubMed:
18647336

19. Identification and analysis of four candidate symbiosis genes from 'Chlorochromatium aggregatum', a highly developed bacterial symbiosis

Author:
Vogl, Kajetan; Wenter, Roland; Dreßen, Martina; Schlickenrieder, Martina; Plöscher, Matthias; Eichacker, Lutz; Overmann, Jörg
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2842-2856
ISSN:
1462-2912
Subject:
gene transfer; proteins; sugars; autoradiography; open reading frames; enzymes; symbiosis; bioinformatics; hybridization; amino acid sequences; Chlorobiales; genes; toxins; tripeptides; bacteria; ethylene glycol tetraacetic acid
Abstract:
... The consortium 'Chlorochromatium aggregatum' currently represents the most highly developed interspecific association between prokaryotes. It consists of green sulfur bacteria, so-called epibionts, which surround a central, motile, chemotrophic bacterium. Four putative symbiosis genes of the epibiont were recovered by suppression subtractive hybridization and bioinformatics approaches. These genes ...
DOI:
10.1111/j.1462-2920.2008.01709.x
PubMed:
18707609

20. Identity and abundance of active sulfate-reducing bacteria in deep tidal flat sediments determined by directed cultivation and CARD-FISH analysis

Author:
Gittel, Antje; Mußmann, Marc; Sass, Henrik; Cypionka, Heribert; Könneke, Martin
Source:
Environmental microbiology 2008 v.10 no.10 pp. 2645-2658
ISSN:
1462-2912
Subject:
Desulfobacter; Desulfobacterium catecholicum; Desulfobacula phenolica; Desulfobulbaceae; Firmicutes; acetates; anaerobes; fluorescence in situ hybridization; hydrogen; nucleotide sequences; nutrients; oligonucleotide probes; ribosomal RNA; sand; sulfate-reducing bacteria; sulfates; sulfides
Abstract:
... The identity and abundance of potentially active sulfate-reducing bacteria (SRB) in several metre deep sediments of a tidal sand flat in the German Wadden Sea were assessed by directed cultivation and cultivation-independent CARD-FISH analysis (catalysed reporter deposition fluorescence in situ hybridization). Presumably abundant SRB from different sediment layers between 0.5 and 4 m depth were se ...
DOI:
10.1111/j.1462-2920.2008.01686.x
PubMed:
18627412