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- Menéndez, Carmen; Martínez, Duniesky; Pérez, Enrique R.; Musacchio, Alexis; Ramírez, Ricardo; López-Munguía, Agustín; Hernández, Lázaro
- Enzyme and microbial technology 2019 v.125 pp. 53-62
- Pichia pastoris; Thermotoga maritima; bacteria; fructooligosaccharides; fructose; gene expression; glucose; glycoproteins; hydrolases; hydrolysis; mutants; mutation; pasteurization; sucrose; syrups; temperature; thermal stability; thermostable enzymes; transferases; yeasts
- ... The thermostable β–fructosidase (BfrA) from the bacterium Thermotoga maritima converts sucrose into glucose, fructose, and low levels of short–chain fructooligosaccharides (FOS) at high substrate concentration (1.75 M) and elevated temperatures (60–70 °C). In this research, FOS produced by BfrA were characterized by HPAE–PAD analysis as a mixture of 1–kestotriose, 6G–kestotriose (neokestose), and ...