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- Lin, Jianping, et al. Show all 6 Authors
- Enzyme and microbial technology 2018 v.115 pp. 45-51
- Corynebacterium glutamicum; amino acid substitution; amino acids; directed evolution; enzyme activity; fumarate hydratase; half life; industrial applications; industrialization; mutants; site-directed mutagenesis; temperature; thermal stability
- ... Fumarases have been successfully applied in industry for the production of l-malate. However, the industrialization of fumarases is limited by their low thermostability. In this study, the thermostability of fumarase C from Corynebacterium glutamicum was enhanced through directed evolution, simulated mutagenesis, site-directed mutagenesis and saturated mutagenesis. Mutant 2G (A411V) was initially ...