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- Arnao, Marino B., et al. Show all 7 Authors
- European journal of biochemistry 2001 v.268 no.10 pp. 3091-3098
- absorption; ascorbate peroxidase; catalytic activity; cytochrome-c peroxidase; digestion; electron paramagnetic resonance spectroscopy; hydrogen peroxide; peas; peroxidase; porphyrins; reversed-phase high performance liquid chromatography; temperature; tryptophan
- ... The reactivity of recombinant pea cytosolic ascorbate peroxidase (rAPX) towards H2O2, the nature of the intermediates and the products of the reaction have been examined using UV/visible and EPR spectroscopies together with HPLC. CompoundâI of rAPX, generated by reaction of rAPX with 1 molar equivalent of H2O2, contains a porphyrin Ïâcation radical. This species is unstable and, in the absenc ...