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- Kato, Ryuichi, et al. Show all 8 Authors
- European journal of biochemistry 2000 v.267 no.4 pp. 1125-1137
- DNA repair; Escherichia coli; activation energy; adenosine triphosphate; adenosinetriphosphatase; genes; homologous recombination; hydrolysis; pH; proteins; single-stranded DNA; temperature
- ... The radA gene is an archaeal homolog of bacterial recA and eukaryotic RAD51 genes, which are critical components in homologous recombination and recombinational DNA repair. We cloned the radA gene from a hyperthermophilic archaeon, Pyrobaculum islandicum, overproduced the radA gene product in Escherichia coli and purified it to homogeneity. The purified P.âislandicum RadA protein maintained its ...