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You searched for: Journal European journal of plant pathology Remove constraint Journal: European journal of plant pathology Publication Year 2013 Remove constraint Publication Year: 2013 Source 2013 v.137 no.2 Remove constraint Source: 2013 v.137 no.2
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1. A nested-polymerase chain reaction protocol for the detection of Mycosphaerella nawae in persimmon

Author:
Berbegal, M.; Mora-Sala, B.; García-Jiménez, J.
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 273-281
ISSN:
0929-1873
Subject:
Mycosphaerella; detection limit; disease surveillance; foliar diseases; internal transcribed spacers; leaves; orchards; pathogens; persimmons; plant litter; polymerase chain reaction; ribosomal DNA
Abstract:
... Mycosphaerella nawae is the causal agent of circular leaf spot of persimmon. A polymerase chain reaction (PCR) based protocol was developed for M. nawae-specific identification from pure culture, or infected symptomatic and asymptomatic persimmon tissues. Variation among the internal transcribed spacer regions (ITS) of the ribosomal DNA (rDNA) sequences of potentially related fungal species in per ...
DOI:
10.1007/s10658-013-0237-0

2. Detection and characterization of fungicide resistant phenotypes of Botrytis cinerea in lettuce crops in Greece

Author:
Chatzidimopoulos, Michael; Papaevaggelou, Dimitris; Pappas, Athanassios Christos
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 363-376
ISSN:
0929-1873
Subject:
Botrytis cinerea; boscalid; carbendazim; chemical control; chlorothalonil; cyprodinil; fenhexamid; fludioxonil; fungicide resistance; greenhouse experimentation; lettuce; pesticide application; phenotype; pyraclostrobin; vegetable crops; Greece
Abstract:
... The development of resistance to chemical control agents needs continuous monitoring in Botrytis cinerea. 790 isolates from lettuce and other vegetable crops were collected from six widely separated sites in Greece and tested for their sensitivity to 11 fungicides from nine unrelated chemical groups. 44 of the isolates exhibited multiple resistance to fenhexamid (hydroxyanilides), azoxystrobin and ...
DOI:
10.1007/s10658-013-0248-x

3. Detection and quantification of Fusarium oxysporum f. sp. cucumerinum in environmental samples using a specific quantitative PCR assay

Author:
Scarlett, Kelly; Tesoriero, Len; Daniel, Rosalie; Guest, David
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 315-324
ISSN:
0929-1873
Subject:
DNA; Fusarium oxysporum; cucumbers; disease control; epidemiology; fungi; pathogens; quantitative polymerase chain reaction; soil; vascular wilt; wilting
Abstract:
... The rapid and reliable identification and quantification of pathogens is essential for the management of economically important plant diseases. Fusarium oxysporum f. sp. cucumerinum is the soil borne fungus responsible for Fusarium vascular wilt of cucumber. In this study, we report the development of a specific and reliable real-time quantitative PCR assay and the development of an ultra-sensitiv ...
DOI:
10.1007/s10658-013-0244-1

4. Detection of streptomycin resistance in Erwinia amylovora strains isolated from apple orchards in Chihuahua, Mexico

Author:
de León Door, Adrian Ponce; Romo Chacón, Alejandro; Acosta Muñiz., Carlos
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 223-229
ISSN:
0929-1873
Subject:
Erwinia amylovora; amino acid substitution; antibiotic resistance; apples; bacteria; chromosomes; enzymes; genes; orchards; pears; plasmids; polymerase chain reaction; streptomycin; Mexico
Abstract:
... Fire blight, one of the most severe diseases of apple and pear, is caused by the bacterium Erwinia amylovora. One control method is the use of antibiotics like streptomycin; however, streptomycin is the only antibiotic registered to control fire blight. A total of 107 E. amylovora strains were isolated from apple orchards located in Cuauhtémoc and Guerrero, Chihuahua, two major apple-producing are ...
DOI:
10.1007/s10658-013-0241-4

5. Development and validation of a set of standard area diagrams to estimate severity of potato early blight

Author:
Duarte, Henrique Silva Silveira; Zambolim, Laércio; Capucho, Alexandre Sandri; Júnior, Antonio Fernandes Nogueira; Rosado, André Wilson Campos; Cardoso, Carine Rezende; Paul, Pierce Anderson; Mizubuti, Eduardo Seiti Gomide
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 249-257
ISSN:
0929-1873
Subject:
Alternaria; Solanum tuberosum; blight; correlation; disease severity; fungicides; image analysis; leaves; potatoes; screening
Abstract:
... Standard area diagrams (SADs) to assess the severity of potato early blight (Alternaria grandis) on leaves of potato (Solanum tuberosum L.) were developed and validated. The proposed SADs include images of leaves with 10 distinct disease severities (0.1, 1, 3, 5, 10, 20, 40, 60, 80 and 100 %). The SADs were validated by 12 raters who had no previous experience in evaluating plant disease. Lin’s co ...
DOI:
10.1007/s10658-013-0234-3

6. Development of a quantitative real-time RT-PCR (qRT-PCR) for the detection of hop stunt viroid

Author:
Luigi, Marta; Faggioli, Francesco
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 231-235
ISSN:
0929-1873
Subject:
Hop stunt viroid; chemistry; peaches; Potato spindle tuber viroid; cachexia; genome; hosts; plant pathogens; Citrus; pears; plums; reverse transcriptase polymerase chain reaction; Pear blister canker viroid; Apple scar skin viroid; Peach latent mosaic viroid; Apple dimple fruit viroid; RNA; apricots
Abstract:
... Hop stunt viroid (HSVd) is a worldwide plant pathogen that infects a wide range of hosts. In some cases different molecular variants are associated with particular disease symptoms, e.g. the cachexia isolate on citrus. A new method for HSVd detection has been developed by designing a new primer pair and a probe for quantitative real-time RT-PCR (qRT-PCR) assay that is based on TaqMan™ chemistry. T ...
DOI:
10.1007/s10658-013-0243-2

7. Development of a specific molecular tool for the detection of epidemiologically active mulberry causing-disease strains of Ralstonia solanacearum phylotype I (historically race 5-biovar 5) in China

Author:
Pan, Z. C.; Xu, J.; Prior, P.; Xu, J. S.; Zhang, H.; Chen, K. Y.; Tian, Q.; Zhang, L. Q.; Liu, L.; He, L. Y.; Feng, J.
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 377-391
ISSN:
0929-1873
Subject:
DNA; Ralstonia solanacearum; bacterial wilt; diagnostic techniques; genome; polymerase chain reaction; suppression subtractive hybridization; wilting; China
Abstract:
... Ralstonia solanacearum causes bacterial wilt disease in many plant species, including mulberry. Here, we used a suppression subtractive hybridization (SSH) approach to identify specific DNA fragments in R. solanacearum race 5-biovar 5. The genome of the R. solanacearum M7 strain was subtracted from that of the GMI1000 strain, resulting in the identification of 85 subtracted fragments. The primer s ...
DOI:
10.1007/s10658-013-0249-9

8. Genotypic variability and aggressiveness of Bipolaris oryzae in the Philippines

Author:
Burgos, M. R. G.; Katimbang, M. L. B.; Dela Paz, M. A. G.; Beligan, G. A.; Goodwin, P. H.; Ona, I. P.; Mauleon, R. P.; Ardales, E. Y.; Vera Cruz, C. M.
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 415-429
ISSN:
0929-1873
Subject:
microsatellite repeats; rice; haplotypes; Bipolaris oryzae; aggression; DNA; genetic variation; minisatellite repeats; pathogens; ecosystems; paddies; leaves; Philippines
Abstract:
... The genotypic diversity of a collection of 352 isolates of Bipolaris oryzae obtained from 11 locations in the Philippines was estimated. The isolates could be divided into 50 haplotypes based on variation in microsatellite DNA with a moderately high genotypic diversity value of 0.88. Thirty nine haplotypes were represented by three or fewer isolates, whereas 80 % of the isolates belonged to only e ...
DOI:
10.1007/s10658-013-0256-x

9. Guava decline: effect of root exudates from Meloidogyne enterolobii-parasitized plants on Fusarium solani in vitro and on growth and development of guava seedlings under controlled conditions

Author:
Gomes, Vicente Martins; Souza, Ricardo Moreira; da Silveira, Silvaldo Felipe; Almeida, Alexandre Macedo
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 393-401
ISSN:
0929-1873
Subject:
Fusarium solani; Meloidogyne; agar; biomass; decline; filtration; freeze drying; fungi; parasitism; plastics; root exudates; root rot; roots; sand; seed germination; seedlings; shoots; trees
Abstract:
... In guava decline, Fusarium solani-immune guava trees become susceptible to extensive root rot caused by this fungus after parasitism by Meloidogyne enterolobii. To understand the mechanisms involved in this disease, root exudates were collected from nematode-inoculated (NI) or uninoculated (UN) guava plants cultivated in sand. After filtration through a Millipore® membrane, NI and UN exudates were ...
DOI:
10.1007/s10658-013-0251-2

10. Influence of light qualities on antifungal lipopeptide synthesis in Bacillus amyloliquefaciens JBC36

Author:
Ramkumar, Gandhimani; Yu, Sang-Mi; Lee, Yong Hoon
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 243-248
ISSN:
0929-1873
Subject:
Bacillus amyloliquefaciens; Citrus unshiu; Penicillium digitatum; antifungal properties; biological control agents; blue light; disease control; gene expression; high performance liquid chromatography; lighting; lipopeptides; quantitative polymerase chain reaction; red light; secondary metabolites; white light
Abstract:
... Bacillus amyloliquefaciens JBC36 (JBC36) is a potent biocontrol agent and was proved to control green and blue molds of Satsuma mandarin (Citrus unshiu). Moreover, light qualities affected its biocontrol efficacy; green and red light significantly increased the antifungal activity of the antagonist in our previous study. The disparity in antagonism on Penicillium digitatum under different light qu ...
DOI:
10.1007/s10658-013-0253-0

11. Molecular identification of a 16SrIII-B phytoplasma associated with cassava witches’ broom disease

Author:
Flôres, Daniela; Haas, Isolda Cristina; Canale, Maria Cristina; Bedendo, Ivan Paulo
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 237-242
ISSN:
0929-1873
Subject:
16SrIII (X-disease group); cassava; deformation; leaves; phylogeny; Brazil
Abstract:
... Cassava plants displaying typical symptoms commonly associated with phytoplasmas, such as witches’ broom, general stunt, leaves with chlorosis, deformation, and reduced size, were observed in fields located in the state of São Paulo, Brazil. Molecular analysis using specific primers evidenced that the phytoplasma found in all positive samples was affiliated with group 16SrIII (X-disease group). Ba ...
DOI:
10.1007/s10658-013-0250-3

12. Multi-gene sequence analysis and phenotypic diversity of Phaeoacremonium species isolated from grapevines in Spain

Author:
Martín, Laura; Martín, María Teresa
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 343-361
ISSN:
0929-1873
Subject:
sequence analysis; vines; translation (genetics); tubulin; decline; polymerase chain reaction; phylogeny; internal transcribed spacers; mature plants; genotype; wine industry; actin; dieback; exons; Spain
Abstract:
... Esca and Petri disease, caused by Phaeoa-cremonium, and other grapevine decline diseases, such as eutypa dieback, are responsible for serious economic losses to the wine industry worldwide. In the present work, 175 isolates of Phaeoacremonium spp. were collected (mostly in the region of Castilla y León, Spain) from vines either asymptomatic or symptomatic for grapevine decline diseases. The sequen ...
DOI:
10.1007/s10658-013-0247-y

13. Multiplex RT-PCR for parallel detection of three RNA viruses associated with stripe and mosaic diseases of sorghum in India

Author:
Srinivas, K. P.; Sreekanth Reddy, M.; Hema, M.; Sreenivasulu, P.
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 211-215
ISSN:
0929-1873
Subject:
Maize stripe virus; RNA; Sugarcane streak mosaic virus; reverse transcriptase polymerase chain reaction; viruses; India
Abstract:
... Stripe of sorghum caused by Maize stripe virus (MSpV-Sorg) and mosaic of sorghum caused by Sugarcane streak mosaic virus (SStMV-Sorg) and a newly identified rhabdovirus (tentatively named Indian sorghum mosaic virus-ISMV) are the two prevalent and important viral diseases of sorghum in India. In this study, a multiplex-reverse transcription-polymerase chain reaction (M-RT-PCR) was developed for th ...
DOI:
10.1007/s10658-013-0236-1

14. Nematicidal potential of Artemisia annua and its main metabolites

Author:
D’Addabbo, Trifone; Carbonara, Teresa; Argentieri, Maria Pia; Radicci, Vincenzo; Leonetti, Paola; Villanova, Luciano; Avato, Pinarosa
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 295-304
ISSN:
0929-1873
Subject:
Artemisia annua; Globodera rostochiensis; Meloidogyne incognita; Xiphinema index; artemisinin; biocides; caffeic acid; chlorogenic acid; cyst nematodes; egg masses; eggs; exposure duration; females; juveniles; metabolites; phytochemicals; potatoes; root-knot nematodes; toxicity
Abstract:
... Nematotoxic effect of an aqueous extract of Artemisia annua and its components caffeic acid, chlorogenic acid (5-caffeoylquinic acid, 5-CQA), artemisinin and the related semi-synthetic artesunate, was investigated on the root-knot nematode Meloidogyne incognita and the potato cyst nematode Globodera rostochiensis and on the virus-vector dagger nematode Xiphinema index. Juveniles of M. incognita an ...
DOI:
10.1007/s10658-013-0240-5

15. Preliminary research on soil conditioner mediated citrus Huanglongbing mitigation in the field in Guangdong, China

Author:
Xu, Meirong; Liang, Meidan; Chen, Jianchi; Xia, Yulu; Zheng, Zheng; Zhu, Qing; Deng, Xiaoling
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 283-293
ISSN:
0929-1873
Subject:
Candidatus Liberibacter asiaticus; Citrus; calcium; fruit yield; greening disease; leaves; mandarins; manganese; orchard soils; orchards; pathogens; shoots; soil conditioners; trees; China
Abstract:
... Huanglongbing (HLB, yellow shoot disease) is devastating citrus production worldwide. No effective control measure is currently available. In China, management of HLB through nutrient applications was used in the past and the interest has been renewed recently. In this study the effects of a soil conditioner (SC) on HLB-affected mandarin citrus (Citrus reticulate Blanco) were evaluated in four orc ...
DOI:
10.1007/s10658-013-0238-z

16. Psathyrostachys huashanica, a potential resource for resistance to Barley yellow dwarf virus-GAV

Author:
Song, Shuang; Tao, Ye; Zhang, Huawei; Wu, Yunfeng
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 217-221
ISSN:
0929-1873
Subject:
Psathyrostachys huashanica; barley; drought tolerance; fungi; germplasm; leaves; pathogens; reverse transcriptase polymerase chain reaction; salinity; viruses; wheat
Abstract:
... Barley/cereal yellow dwarf viruses (B/CYDVs) are recognized as serious pathogens of wheat. It is critical to find diverse sources of resistance to reduce yield losses caused by B/CYDVs. Psathyrostachys huashanica Keng, a perennial wheat-relative species, has been regarded as a potential germplasm resource because of its excellent performance on resistance to drought, salinity, wheat stripe rust an ...
DOI:
10.1007/s10658-013-0239-y

17. Quantitative detection of the root-lesion nematode, Pratylenchus penetrans, using qPCR

Author:
Mokrini, Fouad; Waeyenberge, Lieven; Viaene, Nicole; Abbad Andaloussi, Fouad; Moens, Maurice
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 403-413
ISSN:
0929-1873
Subject:
DNA; Pratylenchus penetrans; annealing; crops; extension education; farmers; genes; pest management; quantitative polymerase chain reaction; root lesion nematodes; taxonomy; temperature
Abstract:
... Pratylenchus penetrans is one of the most economically damaging plant-parasitic nematodes and is found on a wide variety of crops. Correct identification and quantification of this nematode are necessary for providing advice to farmers, but are not easily obtained with the traditional way of microscopic observation. We developed a qPCR assay to detect and quantify P. penetrans in a short but accur ...
DOI:
10.1007/s10658-013-0252-1

18. Real-time PCR quantification and spatio-temporal distribution of airborne inoculum of Mycosphaerella graminicola in Belgium

Author:
Duvivier, Maxime; Dedeurwaerder, Géraldine; De Proft, Michel; Moreau, Jean-Marc; Legrève, Anne
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 325-341
ISSN:
0929-1873
Subject:
Mycosphaerella graminicola; air; ascospores; complementary DNA; correlation; flowering; growing season; inoculum; leaves; quantitative polymerase chain reaction; stem elongation; trapping; traps; Belgium
Abstract:
... Two kinds of propagules play a role in Mycosphaerella graminicola dissemination: splash-dispersed pycnidiospores and airborne sexual ascospores. A method based on real-time polymerase chain reaction (PCR) assay and using Burkard spore traps was developed to quantify M. graminicola airborne inoculum. The method was tested for its reliability and applied in a spore trap network over a 2-year period ...
DOI:
10.1007/s10658-013-0245-0

19. Screening of cotton germplasm for resistance to Verticillium dahlia Kleb. under greenhouse and field conditions

Author:
Khaskheli, M. Ibrahim; Sun, J. Ling; He, S. Pu; Du, X. Ming
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 259-272
ISSN:
0929-1873
Subject:
Dahlia; Verticillium; Verticillium wilt; cotton; crop production; cultivars; disease resistance; germplasm; greenhouses; screening; wilting
Abstract:
... Currently, the most effective and economically feasible means of controlling Verticillium wilt is to grow wilt-resistant cultivars. Nevertheless, resistant germplasm lines that are able to overcome this detrimental disease remain rare. Hence, we aimed to identify high-quality germplasm lines that exhibit high resistance, to address the dearth in optimum production. Our field and greenhouse studies ...
DOI:
10.1007/s10658-013-0235-2

20. Species-specific DNA markers for identification of two root-knot nematodes of coffee: Meloidogyne arabicida and M. izalcoensis

Author:
Correa, Valdir Ribeiro; dos Santos, Marcilene Fernandes Almeida; Almeida, Maria Ritta Alves; Peixoto, José Ricardo; Castagnone-Sereno, Philippe; Carneiro, Regina Maria Dechechi Gomes
Source:
European journal of plant pathology 2013 v.137 no.2 pp. 305-313
ISSN:
0929-1873
Subject:
DNA; Meloidogyne; females; genetic markers; juveniles; males; pathogens; polymerase chain reaction; root-knot nematodes; roots; soil
Abstract:
... Seven root-knot nematodes (RKN), including Meloidogyne exigua, M. incognita, M. paranaensis, M. enterolobii, M. arabicida, M. izalcoensis and M. arenaria are major pathogens of coffee crop in the Americas. Species-specific primers for their identification have been developed for five of them and constitute a fast and reliable method of identification. Here we report a PCR-based assay for specific ...
DOI:
10.1007/s10658-013-0242-3