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... The present study focuses on antifungal potential of 1,2,4-triazole derivatives of allyl sulfides. The antifungal potential of synthesized 2-(allylthio)-1-(1'H-1',2',4'-triazol-1'-yl)ethanone (ATT) was compared with its diallyl analogue (a natural occurring) against Fusarium fujikuroito unveil its improved antifungal potential. The synthesized compound ATT had significantly better antifungal poten ...
... Bakanae disease of rice caused by Fusarium fujikuroi is a significant disease of rice worldwide. The use of inoculum free seeds works well to act against this seed borne disease. Increased restrictions by the environmental protection agency (EPA) on the use of chemical seed-dressing products have made this disease a major concern among seed producers. In the present studies, 1,2,4-triazole derivat ...
... Root drench-treatment of tobacco seedlings with Bacillus sp. BS107 (BS107) suppressed disease development caused by the pathogen Pectobacterium carotovorum subsp. carotovotrum SCC1 (SCC1). A determinant of BS107 involved in induced systemic resistance (ISR) against SCC1 was isolated from cell-free culture. ISR bioassay-guided isolation was involved in determining active fractions during chromatogr ...
... This study aimed at evaluating the ability of 6-pentyl-α-pyrone (6PP) isolated from Trichoderma koningii CTX1172 (AUMC 11520), an endophytic fungal strain to induce systemic resistance in tobacco (Nicotiana tabacum cv. White Burley) plant against tobacco mosaic virus (TMV). 6PP exhibited 10–60% symptoms inhibition at low concentrations (10–30 μg mL⁻¹) achieving 100% biocontrol efficacy at high con ...
... Leptosphaeria maculans, the cause of stem canker of oilseed rape (OSR), exhibits gene-for-gene interactions with its host plant. The race structure of L. maculans was assessed on the basis of the analysis of 1011 isolates collected in France between 1990 and 2000, with regards to three AVR genes, AvrLm1, AvrLm2 and AvrLm4. The effect of selection pressure, due to large-scale cropping of Rlm1 culti ...
... Two binucleate Rhizoctonia (BNR) isolates (HLJ-21 and HLJ-57) were recovered from diseased sugar beet seedlings in Heilongjiang Province, Northeastern China in May, 2015. The two isolates were identified as members of the anastomosis group (AG)-W based on morphological characteristics, hyphal anastomosis, polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP), and phylogenet ...
... Botrytis cinerea (anamorph of Botryotinia fuckeliana) is a filamentous ascomycete that causes grey mould on grapevine. We had previously described two distinct populations, named HydR1 and non-HydR1, that comprise two distinct genetic entities based on genetic polymorphism, natural resistance towards the fungicide hydroxyanilide fenhexamid, and vegetative incompatibility between them. Here, we use ...
... Bursaphelenchus xylophilus is an economically important pathogen that has caused serious damage to pine forests. Fatty acid and retinol-binding proteins (FARs) are nematode-specific proteins. One FAR gene, Bx-FAR-1, was identified from the transcriptome of B. xylophilus as a putative effector. It was upregulated in both highly and weakly virulent isolates in early infection stages. Transient expre ...
... Root-knot nematodes are obligate parasites that invade the roots of agricultural plants and induce the formation of specialized feeding structures, especially races 3 and 4 of the southern root-knot nematode. However, not much is known about the defense mechanisms of plants against the invasion of M. incognita race 1. In this study, we characterized and performed functional analysis of the CC-NBS- ...
... Nine avirulence genes (AvrLm1-AvrLm9) were identified in Leptosphaeria maculans, the causal agent of stem canker of oilseed rape (OSR), combinations of which could theoretically generate up to 512 different races of the fungus. L. maculans displays a high evolutionary potential to adapt to novel resistance genes as illustrated by the Rlm1 breakdown in France, where virulent populations became prev ...
... Fusarium graminearum and Zymoseptoria tritici cause economically important diseases of wheat. F. graminearum is one of the primary causal agents of Fusarium head blight (FHB) and Z. tritici is the causal agent of Septoria tritici blotch (STB). Alternative control methods are required in the face of fungicide resistance and EU legislation which seek to cut pesticide use by 2030. Both fungal pathoge ...
... The cereal root-knot nematode Meloidogyne naasi can cause serious cereal crop losses. The nematode is also found in agricultural fields where non-host crops are grown. Control of M. naasi can be based on preventing its spread, host resistance and crop management as well as on the design of crop rotation systems. Detection methods are required for these purposes and can also be helpful for inspecti ...
... The partial nucleotide sequence of the RNA polymerase gene from one isolate of Pepino mosaic virus (PepMV) was determined. Phylogenetic and distance analysis indicated that this isolate was related to other isolates of PepMV previously reported. To develop a method for detecting PepMV by reverse transcriptase-polymerase chain reaction (RT-PCR), a pair of primers was designated from RNA polymerase ...
... Zeamines are family of potent antibiotics and virulence determinants produced by the rice foot rot bacterial pathogen Dickeya zeae. So zeamines are important for the pathogenesis of D. zeae and development of new strategies against this devastating disease. In this study, we show that production of zeamines is positively modulated by ZmsO, which is a conserved Sfp-type phosphopantetheinyl thransfe ...
... Rhizoctonia cerealis causes sharp eyespot in cereals and the pathogen survives as mycelia or sclerotia in soil. Real-time Polymerase Chain Reaction (qPCR) assays based on TaqMan chemistry are highly suitable for use on DNA extracted from soil. We report here the first qPCR assay for R. cerealis using TaqMan primers and a probe based on a unique Sequence Characterised Amplified Region (SCAR). The a ...
... Watermelon mosaic virus (WMV), a plus-strand RNA virus that belongs to the family Potyviridae, is one of the most damaging viruses that infect cucurbit crops. However, isolates of this species frequently induce mild symptoms, which makes difficult to manage the disease in the field and breeding programs. A new generation of marker genes has been recently developed to visually track plant virus inf ...
... ‘Gold Veined Oxalis’ (Oxalis debilis) is an ornamental plant cultivar with attractive yellow vein foliage. After grafting scions of this plant to O. debilis plants that lacked the yellow vein foliage, grafted plants developed yellow vein suggesting that an infectious agent was the cause of the yellow vein. DNA extracts from O. debilis plants showing yellow vein were used in rolling circle amplific ...
... The root knot nematode Meloidogyne javanicaa plant parasite that is an agricultural pest, establishes and maintains a permanent feeding site within plant roots. In order to gain a broad view of gene expression in nematode feeding sites during the compatible response of tomato to root knot nematodes, we used the tomato spotted microarray chip, followed by real-time reverse transcription polymerase ...