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You searched for: Journal name Experimental parasitology Remove constraint Journal name: Experimental parasitology Publication year rev 7984-2016 Remove constraint Publication year rev: 7984-2016 Source 2016 v.162 Remove constraint Source: 2016 v.162
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1. Co-administration of interleukins 7 and 15 with DNA vaccine improves protective immunity against Toxoplasma gondii

Author:
Jia Chen; Zhong-Yuan Li; Eskild Petersen; Wen-Ge Liu; Xing-Quan Zhu
Source:
Experimental parasitology 2016 v.162 pp. 18-23
ISSN:
0014-4894
Subject:
CD4-positive T-lymphocytes; Toxoplasma gondii; antibodies; blood serum; brain; cell-mediated immunity; cytotoxicity; flow cytometry; homeostasis; humans; immune response; immunoglobulin G; interferon-gamma; interleukin-15; interleukin-2; interleukin-7; mice; parasites; plasmids; protein kinases; recombinant vaccines; tissues
Abstract:
... Toxoplasma gondii is an obligatory intracellular parasite, which can infect all warm-blooded animals including humans. Cytokines, including IL-15 and IL-7, play a critical role in the regulation of the homeostasis of naive and memory T cells. Co-administration the DNA vaccine with cytokines may improve its efficacy. IL-7 and IL-15 from splenic tissues of Kunming mice were cloned, and eukaryotic pl ...
DOI:
10.1016/j.exppara.2015.12.013

2. DsRNA-mediated silencing of Nudix hydrolase in Trichinella spiralis inhibits the larval invasion and survival in mice

Author:
Shuai Bing Zhang; Peng Jiang; Zhong Quan Wang; Shao Rong Long; Ruo Dan Liu; Xi Zhang; Wei Yang; Hui Jun Ren; Jing Cui
Source:
Experimental parasitology 2016 v.162 pp. 35-42
ISSN:
0014-4894
Subject:
RNA interference; Trichinella spiralis; Western blotting; adults; dose response; double-stranded RNA; electroporation; genes; intestinal mucosa; larval development; mice; muscle larvae; pathogenicity; quantitative polymerase chain reaction; soaking
Abstract:
... The aim of this study was to investigate the functions of Trichinella spiralis Nudix hydrolase (TsNd) during the larval invasion of intestinal epithelial cells (IECs), development and survival in host by RNAi. The TsNd-specific double-stranded RNA (dsRNA) was designed to silence the expression of TsNd in T. spiralis larvae. DsRNA were delivered to the larvae by soaking incubation or electroporatio ...
DOI:
10.1016/j.exppara.2016.01.005

3. In vitro and in vivo action of terpinen-4-ol, γ-terpinene, and α-terpinene against Trypanosoma evansi

Author:
Matheus D. Baldissera; Thirssa H. Grando; Carine F. Souza; Lucas T. Gressler; Lenita M. Stefani; Aleksandro S. da Silva; Silvia G. Monteiro
Source:
Experimental parasitology 2016 v.162 pp. 43-48
ISSN:
0014-4894
Subject:
Melaleuca alternifolia; Trypanosoma evansi; diminazene; drug therapy; essential oils; gamma-terpinene; in vivo studies; longevity; mice; tea tree oil; trees; trypanosomiasis
Abstract:
... This study aimed to evaluate the susceptibility in vitro and in vivo of Trypanosoma evansi to terpinen-4-ol, γ-terpinene and α-terpinene, the three main compounds of tea tree oil (Melaleuca alternifolia) with known efficacy in the treatment of trypanosomosis. In vitro, a trypanocidal effect of terpinen-4-ol, γ-terpinene, and α-terpinene was observed when used alone or associated at 0.5, 1 and 2% c ...
DOI:
10.1016/j.exppara.2016.01.004

4. Cryptosporidium parvum and Enterocytozoon bieneusi in American Mustangs and Chincoteague ponies

Author:
Pavla Wagnerová; Bohumil Sak; John McEvoy; Michael Rost; Dawn Sherwood; Kevin Holcomb; Martin Kváč
Source:
Experimental parasitology 2016 v.162 pp. 24-27
ISSN:
0014-4894
Subject:
Chincoteague Pony; Cryptosporidium parvum; Encephalitozoon; Enterocytozoon bieneusi; diarrhea; feces; feral animals; genes; genotype; glycoproteins; horses; humans; internal transcribed spacers; loci; parasites; pathogens; ribosomal RNA; sequence analysis; United States
Abstract:
... The prevalence of Cryptosporidium and microsporidia in feral horses, which have minimal contact with livestock and humans, is not currently known. We report the findings of a study on Cryptosporidium and microsporidia in 34 Mustangs and 50 Chincoteague ponies in the USA. Fecal samples were screened for presence of Cryptosporidium spp. by analysis of the small-subunit rRNA (SSU) and 60-kDa glycopro ...
DOI:
10.1016/j.exppara.2015.12.004

5. Development of resazurin-based assay in 384-well format for high throughput whole cell screening of Trypanosoma brucei rhodesiense strain STIB 900 for the identification of potential anti-trypanosomal agents

Author:
Kah Tee Lim; Zuriati Zahari; Azimah Amanah; Zafarina Zainuddin; Mohd Ilham Adenan
Source:
Experimental parasitology 2016 v.162 pp. 49-56
ISSN:
0014-4894
Subject:
African trypanosomiasis; Trypanosoma rhodesiense; automation; cost effectiveness; dimethyl sulfoxide; drugs; fluorescence; humans; liquids; screening
Abstract:
... To accelerate the discovery of novel leads for the treatment of Human African Trypanosomiasis (HAT), it is necessary to have a simple, robust and cost-effective assay to identify positive hits by high throughput whole cell screening. Most of the fluorescence assay was made in black plate however in this study the HTS assay developed in 384-well format using clear plate and black plate, for compari ...
DOI:
10.1016/j.exppara.2016.01.002

6. Identification and immunogenicity of microneme protein 2 (EbMIC2) of Eimeria brunetti

Author:
Tran Duc Hoan; Zhenchao Zhang; Jingwei Huang; Ruofeng Yan; Xiaokai Song; Lixin Xu; Xiangrui Li
Source:
Experimental parasitology 2016 v.162 pp. 7-17
ISSN:
0014-4894
Subject:
Eimeria brunetti; Escherichia coli; amino acid sequences; antibodies; antigens; antiserum; blood serum; chickens; coccidiostats; cytokines; genes; immunogenicity; immunoglobulin G; nucleotide sequences; oocysts; plasmids; polyacrylamide gel electrophoresis; rats; recombinant proteins; reverse transcriptase polymerase chain reaction; sporozoites; vaccine development
Abstract:
... There have been only a few antigen genes of Eimeria brunetti reported up to now. In this study, the gene encoding the microneme protein 2 (EbMIC2) was isolated from oocysts of E. brunetti by RT-PCR and the immunogenicity of recombinant EbMIC2 was observed. The EbMIC2 was cloned into vector pMD19-T for sequencing. The sequence was compared with the published EbMIC2 gene from GenBank revealed homolo ...
DOI:
10.1016/j.exppara.2015.12.015

7. Mixture of Alum – Naloxone and Alum – Naltrexone as a novel adjuvant elicits immune responses for Toxoplasma gondii lysate antigen in BALB /c mice

Author:
Zohre Khorshidvand; Shahram Shahabi; Habib Mohamadzade; Ahmad Daryani; Khosrow Hazrati Tappeh
Source:
Experimental parasitology 2016 v.162 pp. 28-34
ISSN:
0014-4894
Subject:
Toxoplasma gondii; adjuvants; adverse effects; alum; aluminum; antigens; cell-mediated immunity; delayed hypersensitivity; immune response; immunization; interferon-gamma; interleukin-5; mice; models; naloxone; parasites; toxicity; vaccine development; vaccines
Abstract:
... Toxoplasma gondii (T. gondii) is an obligate intracellular parasite. Treatment of the infection induced by this parasite is not straightforward due to the toxic side effects of the available drugs. Vaccine development could be a solution to this problem. In the present study, T.gondii Lysate Antigen (TLA), as a model vaccine, in combination with the Alum-NLT (Aluminum phosphate-Naltrexone) and Alu ...
DOI:
10.1016/j.exppara.2016.01.001

8. Sexual transmission of Trypanosoma cruzi in murine model

Author:
Marcelle Ribeiro; Nadjar Nitz; Camilla Santana; Aline Moraes; Luciana Hagström; Rafael Andrade; Adriano Rios; Alessandro Sousa; Bruno Dallago; Rodrigo Gurgel-Gonçalves; Mariana Hecht
Source:
Experimental parasitology 2016 v.162 pp. 1-6
ISSN:
0014-4894
Subject:
Chagas disease; Triatominae; Trypanosoma cruzi; animal models; blood; blood transfusion; females; males; mice; parasites; tissues
Abstract:
... Trypanosoma cruzi is mainly transmitted by blood-sucking triatomines, but other routes also have epidemiological importance, such as blood transfusion and congenital transmission. Although the possibility of sexual transmission of T. cruzi has been suggested since its discovery, few studies have been published on this subject. We investigated acquisition of T. cruzi by sexual intercourse in an exp ...
DOI:
10.1016/j.exppara.2015.12.017

9. TGF-β1 levels and intraocular tissue alterations in mice infected with a virulent type I RH Toxoplasma gondii strain

Author:
Jamshaid Iqbal; Mohammad Ahmed Al-Awadhi; Raj Gopal Raghupathy
Source:
Experimental parasitology 2016 v.162 pp. 57-63
ISSN:
0014-4894
Subject:
Toxoplasma gondii; adults; blindness; blood; cell-mediated immunity; disease severity; epithelium; genetics; immune response; inflammation; interferon-gamma; mice; morbidity; mortality; parasites; tachyzoites; toxoplasmosis; transforming growth factor beta 1; tumor necrosis factor-alpha; virulence
Abstract:
... Toxoplasmosis is generally self-limiting in healthy adults but it may cause toxoplasmic retinochoroiditis in cases of congenital infection leading to blindness. The importance of host genetics in determining disease severity in ocular toxoplasmosis has been shown in different inbred mouse strains using low-virulence toxoplasma strain. In this study, we studied intraocular immune response and tissu ...
DOI:
10.1016/j.exppara.2016.01.003