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You searched for: Journal Experimental parasitology Remove constraint Journal: Experimental parasitology Publication Year 2016 Remove constraint Publication Year: 2016 Source 2016 v.170 Remove constraint Source: 2016 v.170
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1. A nanotechnology based new approach for Trypanosoma evansi chemotherapy: In vitro and vivo trypanocidal effect of (-)-α-bisabolol

Author:
Baldissera, Matheus D.; Grando, Thirssa H.; de Souza, Carine F.; Cossetin, Luciana F.; da Silva, Ana P.T.; Giongo, Janince L.; Monteiro, Silvia G.
Source:
Experimental parasitology 2016 v.170 pp. 156-160
ISSN:
0014-4894
Subject:
Trypanosoma evansi; alpha-bisabolol; diminazene; drug therapy; lipids; longevity; mice; nanoparticles; prepatent period
Abstract:
... The aim of this study was to evaluate the in vitro and in vivo susceptibility of Trypanosoma evansi to α-Bisabolol and solid lipid nanoparticles containing α-Bisabolol (SLN-B). In vitro, a trypanocidal effect of α-Bisabolol and SLN-B was observed when used at 0.5, 1 and 2% concentrations, i.e., the concentrations of 1 and 2% showed a faster trypanocidal effect when compared to chemotherapy (dimina ...
DOI:
10.1016/j.exppara.2016.09.018

2. Anti Leishmania activity of Lucilia sericata and Calliphora vicina maggots in laboratory models

Author:
Sanei-Dehkordi, Alireza; Khamesipour, Ali; Akbarzadeh, Kamran; Akhavan, Amir Ahmad; Mir Amin Mohammadi, Akram; Mohammadi, Younes; Rassi, Yavar; Oshaghi, Mohammad Ali; Alebrahim, Zahra; Eskandari, Seyed Ebrahim; Rafinejad, Javad
Source:
Experimental parasitology 2016 v.170 pp. 59-65
ISSN:
0014-4894
Subject:
Calliphora vicina; Leishmania major; Lucilia sericata; amastigotes; anti-infective properties; bacteria; cutaneous leishmaniasis; debridement; excretion; in vivo studies; insect larvae; macrophages; mechanism of action; mice; models; protective effect; salivary glands; secretion; skin lesions
Abstract:
... Use of sterile fly larvae (maggots) of blow flies for the treatment of many different types of skin and soft tissue wounds is called Maggot debridement therapy (MDT). The larvae of blow flies secrete a broad spectrum of compounds with diverse mechanisms of action in the gut and salivary glands called excretion/secretion (ES) products which showed to have antimicrobial activities against Gram negat ...
DOI:
10.1016/j.exppara.2016.08.007

3. Biocompatible silver nanoparticles reduced from Anethum graveolens leaf extract augments the antileishmanial efficacy of miltefosine

Author:
Kalangi, Suresh K.; Dayakar, A.; Gangappa, D.; Sathyavathi, R.; Maurya, R.S.; Narayana Rao, D.
Source:
Experimental parasitology 2016 v.170 pp. 184-192
ISSN:
0014-4894
Subject:
Anethum graveolens; DNA; DNA fragmentation; Fourier transform infrared spectroscopy; Leishmania; X-ray diffraction; antiparasitic properties; crystal structure; dill; drug delivery systems; drug therapy; drugs; humans; inhibitory concentration 50; leaf extracts; leishmaniasis; macrophages; nanosilver; parasites; promastigotes; reducing agents; scanning electron microscopy; toxicity; transmission electron microscopy; viability
Abstract:
... Despite the existence of chemotherapy, there is no effective cure for leishmaniasis. In the light of recommended therapeutic regimen is attributed for toxicity and development of clinical resistance, exploration of an efficient method of drug delivery could be one of the option in reducing the dosage and toxicity of drugs. This work is aimed in such fashion to study the enhanced antileishmanial ac ...
DOI:
10.1016/j.exppara.2016.09.002

4. Calcium-dependent microneme protein discharge and in vitro egress of Eimeria tenella sporozoites

Author:
Yan, Xinlei; Tao, Geru; Liu, Xianyong; Ji, Yongsheng; Suo, Xun
Source:
Experimental parasitology 2016 v.170 pp. 193-197
ISSN:
0014-4894
Subject:
Eimeria tenella; acetaldehyde; calcium; chickens; hosts; kidney cells; parasites; secretion; sporozoites
Abstract:
... Egress is a vital step in the endogenous development of apicomplexan parasites, as it assures the parasites exit from consumed host cells and entry into fresh ones. However, little information has previously been reported on this step of Eimeria spp. In this study, we investigated in vitro egress of Eimeria tenella sporozoites triggered by acetaldehyde. We found that addition of exogenous acetalde ...
DOI:
10.1016/j.exppara.2016.09.005

5. Characterisation of a new, highly effective method for detecting nematode eggs (Ascaris spp., Toxocara spp., Trichuris spp.) in sewage sludge containing flocculants

Author:
Zdybel, Jolanta; Karamon, Jacek; Różycki, Mirosław; Bilska-Zając, Ewa; Kłapeć, Teresa; Cencek, Tomasz
Source:
Experimental parasitology 2016 v.170 pp. 198-206
ISSN:
0014-4894
Subject:
Ascaris suum; Toxocara canis; Trichuris vulpis; detection limit; eggs; electrolytes; flocculants; parasites; regression analysis; sewage sludge; surfactants
Abstract:
... Because traditional methods used for sewage sludge parasitological examinations have low sensitivity, a new, highly effective method (own method – OM) was devised. The principle of this method is to eliminate the flocculent effect on the structure of sewage sludge by mechanically damaging floccules in the presence of surfactants and to increase the effectiveness of egg isolation processes in large ...
DOI:
10.1016/j.exppara.2016.09.011

6. Correlation between the virulence of T. cruzi strains, complement regulatory protein expression levels, and the ability to elicit lytic antibody production

Author:
Henrique, Priscila Moraes; Marques, Tatiane; da Silva, Marcos Vinícius; Nascentes, Gabriel Antônio Nogueira; de Oliveira, Carlo Freire; Rodrigues, Virmondes; Gómez-Hernández, César; Norris, Karen Ann; Ramirez, Luis Eduardo; Meira, Wendell Sérgio Ferreira
Source:
Experimental parasitology 2016 v.170 pp. 66-72
ISSN:
0014-4894
Subject:
Trypanosoma cruzi; animal models; antibodies; antibody formation; complement; flow cytometry; host-parasite relationships; mice; parasites; protein synthesis; regulatory proteins; surface proteins; trypomastigotes; virulence; virulent strains
Abstract:
... Trypanosoma cruzi trypomastigotes are able to resist lysis via the complement system, which involves many surface proteins including the complement regulatory protein (CRP). To examine the diversity in CRP recognition among strains of T. cruzi, the expression levels of the translated protein on trypomastigote surfaces were analyzed by flow cytometry, and associations between protein expression and ...
DOI:
10.1016/j.exppara.2016.09.001

7. Cryptosporidium species and Cryptosporidium parvum subtypes in dairy calves and goat kids reared under traditional farming systems in Turkey

Author:
Taylan-Ozkan, Aysegul; Yasa-Duru, Sibel; Usluca, Selma; Lysen, Colleen; Ye, Jianbin; Roellig, Dawn M.; Feng, Yaoyu; Xiao, Lihua
Source:
Experimental parasitology 2016 v.170 pp. 16-20
ISSN:
0014-4894
Subject:
Cryptosporidium parvum; Cryptosporidium ryanae; DNA; cow-calf operations; cryptosporidiosis; dairy calves; diarrhea; farms; genes; genotyping; glycoproteins; humans; kids (goats); management systems; nucleotide sequences; pathogens; rearing; ribosomal RNA; sequence analysis; traditional farming; Turkey (country)
Abstract:
... Molecular characterizations of Cryptosporidium spp. in ruminants reared under traditional animal management systems are scarce and studies conducted thus far have revealed largely an absence of the pathogenic and zoonotic species Cryptosporidium parvum in pre-weaned animals. In this study, we examined Cryptosporidium species and subtype distribution in free-range pre-weaned dairy calves and goat k ...
DOI:
10.1016/j.exppara.2016.06.014

8. Cytokine gene expression and pathology in mice experimentally infected with different isolates of Trypanosoma evansi

Author:
Krishnamoorthy, P.; Sengupta, P.P.; Das, Sangita; Ligi, M.; Shome, B.R.; Rahman, H.
Source:
Experimental parasitology 2016 v.170 pp. 168-176
ISSN:
0014-4894
Subject:
DNA; Trypanosoma evansi; blood; blood vessels; buffaloes; dogs; gene expression; genes; heart; histopathology; interferon-gamma; interleukin-10; interleukin-12; interleukin-2; interleukin-4; interleukin-6; kidneys; liver; mice; muscles; parasites; pathogenicity; polymerase chain reaction; spleen; splenomegaly; testes; tumor necrosis factor-alpha
Abstract:
... Aim of the present study was to assess the cytokine gene expression in liver, kidney and spleen and histopathological changes in mice infected with buffalo and dog isolates of Trypanosoma evansi. Forty-four Swiss albino mice was divided into eleven groups of four mice each and injected subcutaneously with 1 × 105 trypanosomes of buffalo and dog isolate to twenty mice each, four mice served as cont ...
DOI:
10.1016/j.exppara.2016.09.019

9. Development and evaluation of molecular methods for detection of Cryptosporidium spp. in human clinical samples

Author:
Khalil, Shehla; Mirdha, Bijay Ranjan; Paul, Jaishree; Panda, Ashutosh; Makharia, Govind; Chaudhry, Rama; Bhatnagar, Shinjini
Source:
Experimental parasitology 2016 v.170 pp. 207-213
ISSN:
0014-4894
Subject:
Cryptosporidium; DNA; Protozoa; cryptosporidiosis; digestive system diseases; digoxigenin; humans; microscopy; monitoring; nucleic acid hybridization; parasites; quantitative polymerase chain reaction
Abstract:
... Cryptosporidiosis is predominantly a gastrointestinal disease of humans and other animals, caused by various species of protozoan parasites representing the genus Cryptosporidium. Detection of Cryptosporidium spp. in human clinical samples is central to the prevention, surveillance and control of cryptosporidiosis, particularly given that there is presently no broadly applicable treatment regimen ...
DOI:
10.1016/j.exppara.2016.10.001

10. Effective anti-leishmanial activity of minimalist squaramide-based compounds

Author:
Marín, Clotilde; Ximenis, Marta; Ramirez-Macías, Inmaculada; Rotger, Carmen; Urbanova, Kristina; Olmo, Francisco; Martín-Escolano, Rubén; Rosales, María José; Cañas, Rocio; Gutierrez-Sánchez, Ramón; Costa, Antonio; Sánchez-Moreno, Manuel
Source:
Experimental parasitology 2016 v.170 pp. 36-49
ISSN:
0014-4894
Subject:
Leishmania braziliensis; Leishmania donovani; Leishmania infantum; antileishmanials; antiparasitic properties; cytotoxicity; drugs; excretion; hydrogen bonding; macrophages; metabolites; microbodies; mitochondria; parasites; pathogenicity; promastigotes; transmission electron microscopy
Abstract:
... In order to evaluate the in vitro leishmanicidal activity of N,N′-Squaramides derivatives, compounds that feature both hydrogen bond donor and acceptor groups and are capable of multiple interactions with complementary sites, against Leishmania infantum, Leishmania braziliensis and Leishmania donovani a series of 18compounds was prepared and assayed on extracellular and intracellular parasite form ...
DOI:
10.1016/j.exppara.2016.07.013

11. Eimeria spp. infecting quenda (Isoodon obesulus) in the greater Perth region, Western Australia

Author:
Hillman, Alison E.; Yang, Rongchang; Lymbery, Alan J.; Thompson, R.C. Andrew
Source:
Experimental parasitology 2016 v.170 pp. 148-155
ISSN:
0014-4894
Subject:
Eimeria; Isoodon obesulus; cytochrome-c oxidase; domestic animals; humans; loci; mitochondria; parasites; phylogeny; public health; ribosomal RNA; wildlife; Western Australia
Abstract:
... Parasites of wildlife inhabiting urbanised and peri-urban environments are of interest regarding wildlife population health, and also veterinary public health in the case of parasites that can also infect humans and domestic animals. This study aimed to: identify, and estimate the prevalence of, species of Eimeria parasitic in quenda (Isoodon obesulus) in the greater Perth region, Western Australi ...
DOI:
10.1016/j.exppara.2016.09.012

12. Endothelial cells release soluble factors that support the long-term survival of filarial worms in vitro

Author:
Evans, Holly; Flynn, Alexander Francis; Mitre, Edward
Source:
Experimental parasitology 2016 v.170 pp. 50-58
ISSN:
0014-4894
Subject:
Litomosoides sigmodontis; adults; coculture; dialysis; endothelial cells; fetal bovine serum; gerbils; heat; heat inactivation; heat stability; lipids; mice; microfilariae; nematicides; nutrients; parasites; screening
Abstract:
... The inability to maintain filarial nematodes in long-term in vitro culture greatly limits research into the basic biology of these parasites and hinders in vitro screening of novel anti-filarial agents. In this study, we sought to characterize nutrients that promote the long-term survival of filarial worms in vitro. Using microfilariae (MF) obtained from gerbils infected with Litomosoides sigmodon ...
DOI:
10.1016/j.exppara.2016.08.004
PubMed:
27565719
PubMed Central:
PMC5115618

13. Experimental infection of calves with Haemonchus placei or Haemonchus contortus: Assessment of clinical, hematological and biochemical parameters and histopathological characteristics of abomasums

Author:
Fávero, Flávia Carolina; Buzzulini, Carolina; Cruz, Breno Cayeiro; Felippelli, Gustavo; Maciel, Willian Giquelin; Salatta, Bruna; Siniscalchi, Débora; Lopes, Welber Daniel Zanetti; Teixeira, Weslen Fabricio Pires; Soares, Vando Edésio; de Oliveira, Gilson Pereira; da Costa, Alvimar José
Source:
Experimental parasitology 2016 v.170 pp. 125-134
ISSN:
0014-4894
Subject:
Haemonchus contortus; Haemonchus placei; Holstein; adverse effects; calves; color; edema; euthanasia; helminths; hemorrhage; histopathology; hyperplasia; hypertrophy; males; mixed grazing; mucosa; neonates; placebos; sheep; staining
Abstract:
... The present study had the primary objective of evaluating clinical, hematological and biochemical parameters, as well as observing anatomical and histopathological characteristics of abomasums, from calves prime-infected with Haemonchus contortus or H. placei. Ten male Holstein newborns were subdivided in three groups (GI placebo; GII infected with H. contortus; GIII inoculated with H. placei). Ey ...
DOI:
10.1016/j.exppara.2016.09.017

14. Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population

Author:
Carlos, Bianca C.; Fotoran, Wesley L.; Menezes, Maria J.; Cabral, Fernanda J.; Bastos, Marcele F.; Costa, Fabio T.M.; Sousa-Neto, Jayme A.; Ribolla, Paulo E.M.; Wunderlich, Gerhard; Ferreira, Marcelo U.
Source:
Experimental parasitology 2016 v.170 pp. 90-99
ISSN:
0014-4894
Subject:
surface antigens; Plasmodium falciparum; correlation; cross reaction; correlated responses; cumulative exposure; parasites; antibodies; expressed sequence tags; erythrocytes; indigenous species; antigenic variation; malaria
Abstract:
... The var gene-encoded erythrocyte membrane protein-1 of Plasmodium falciparum (PfEMP-1) is the main variant surface antigen (VSA) expressed on infected erythrocytes. The rate at which antibody responses to VSA expressed by circulating parasites are acquired depends on the size of the local VSA repertoire and the frequency of exposure to new VSA. Because parasites from areas with declining malaria e ...
DOI:
10.1016/j.exppara.2016.09.006

15. FhCaBP1 (FH22): A Fasciola hepatica calcium-binding protein with EF-hand and dynein light chain domains

Author:
Cheung, Sarah; Thomas, Charlotte M.; Timson, David J.
Source:
Experimental parasitology 2016 v.170 pp. 109-115
ISSN:
0014-4894
Subject:
Fasciola hepatica; antagonists; barium; binding sites; cadmium; calcium; calmodulin; cations; chlorpromazine; cobalt; copper; denaturation; dynein ATPase; gel electrophoresis; iron; lead; liver flukes; magnesium; manganese; models; nickel; potassium; protein conformation; strontium; trifluoperazine; zinc
Abstract:
... FH22 has been previously identified as a calcium-binding protein from the common liver fluke, Fasciola hepatica. It is part of a family of at least four proteins in this organism which combine an EF-hand containing N-terminal domain with a C-terminal dynein light chain-like domain. Here we report further biochemical properties of FH22, which we propose should be renamed FhCaBP1 for consistency wit ...
DOI:
10.1016/j.exppara.2016.09.013

16. Hepatic injury associated with Trypanosoma cruzi infection is attenuated by treatment with 15-deoxy-Δ12,14 prostaglandin J2

Author:
Penas, Federico Nicolás; Cevey, Ágata Carolina; Siffo, Sofía; Mirkin, Gerardo Ariel; Goren, Nora Beatriz
Source:
Experimental parasitology 2016 v.170 pp. 100-108
ISSN:
0014-4894
Subject:
Chagas disease; Trypanosoma cruzi; agonists; anti-inflammatory activity; collagen; cytokines; enzymes; etiological agents; fibrosis; heart; inflammation; liver; liver function; metabolism; mice; peroxisome proliferator-activated receptors; prostaglandins; tissues; transcription factor NF-kappa B
Abstract:
... Trypanosoma cruzi, the etiological agent of Chagas' disease, causes an intense inflammatory response in several tissues, including the liver. Since this organ is central to metabolism, its infection may be reflected in the outcome of the disease. 15-deoxy-Δ12,14 prostaglandin J2 (15dPGJ2), a natural agonist of peroxisome-proliferator activated receptor (PPAR) γ, has been shown to exert anti-inflam ...
DOI:
10.1016/j.exppara.2016.09.015

17. Identification and functional study of a novel 2-cys peroxiredoxin (BmTPx-1) of Babesia microti

Author:
Zhang, Houshuang; Wang, Zhonghua; Gong, Haiyan; Cao, Jie; Zhou, Yongzhi; Zhou, Jinlin
Source:
Experimental parasitology 2016 v.170 pp. 21-27
ISSN:
0014-4894
Subject:
prokaryotic cells; parasites; babesiosis; electrons; complementary DNA; humans; oxidative stress; hydrogen peroxide; peroxiredoxin; erythrocytic stage; eukaryotic cells; pathogens; oxidation; Babesia microti; histidine; Escherichia coli
Abstract:
... Babesia microti is an emerging human pathogen and the primary causative agent of human babesiosis in many regions of the world. Although the peroxiredoxins (Prxs) or thioredoxin peroxidases (TPx) enzymes of this parasite have been sequenced and annotated, their biological properties remain largely unknown. Prxs are a family of antioxidant enzymes that protect biological molecules against metabolic ...
DOI:
10.1016/j.exppara.2016.08.005

18. Identification and typing of free-living Acanthamoeba spp. by MALDI-TOF MS Biotyper

Author:
Del Chierico, Federica; Di Cave, David; Accardi, Cristel; Santoro, Maristella; Masotti, Andrea; D'Alfonso, Rossella; Berrilli, Federica; Urbani, Andrea; Putignani, Lorenza
Source:
Experimental parasitology 2016 v.170 pp. 82-89
ISSN:
0014-4894
Subject:
Acanthamoeba; agar; cluster analysis; databases; encephalitis; genes; genotype; genotyping; humans; keratitis; matrix-assisted laser desorption-ionization mass spectrometry; pathogenicity; phylogeny; polymerase chain reaction; ribosomal DNA; sequence analysis
Abstract:
... Over the years, the potential pathogenicity of Acanthamoeba for humans and animals has gained increasing attention from the scientific community. More than 24 species belong to this genus, however only some of them are causative agents of keratitis and encephalitis in humans. Due to technical difficulties in diagnosis, these infections are likely to be under-detected. The introduction of 18S rDNA ...
DOI:
10.1016/j.exppara.2016.09.007

19. Identification of immunogenic proteins from ovarian tissue and recognized in larval extracts of Rhipicephalus (Boophilus) microplus, through an immunoproteomic approach

Author:
Ramírez Rodríguez, Patricia Berenice; Rosario Cruz, Rodrigo; Domínguez García, Delia Inés; Hernández Gutiérrez, Rodolfo; Lagunes Quintanilla, Rodolfo Esteban; Ortuño Sahagún, Daniel; González Castillo, Celia; Gutiérrez Ortega, Abel; Herrera Rodríguez, Sara Elisa; Vallejo Cardona, Adriana; Martínez Velázquez, Moisés
Source:
Experimental parasitology 2016 v.170 pp. 227-235
ISSN:
0014-4894
Subject:
Rhipicephalus microplus; acaricides; adjuvants; animal ovaries; antigens; bioinformatics; cathepsins; cattle; humoral immunity; immunization; immunoglobulin G; larvae; microorganisms; pollution; polypeptides; tick control; ticks; vaccines; vitellogenin
Abstract:
... Rhipicephalus (Boophilus) microplus ticks are obligatory hematophagous ectoparasites of cattle and act as vectors for disease-causing microorganisms. Conventional tick control is based on the use of chemical acaricides; however, their uncontrolled use has increased tSresistant tick populations, as well as food and environmental contamination. Alternative immunological tick control has shown to be ...
DOI:
10.1016/j.exppara.2016.10.005

20. Invasion by Trichinella spiralis infective larvae affects the levels of inflammatory cytokines in intestinal epithelial cells in vitro

Author:
Ming, Liang; Peng, Ruo Yu; Zhang, Lei; Zhang, Chun Li; Lv, Pin; Wang, Zhong Quan; Cui, Jing; Ren, Hui Jun
Source:
Experimental parasitology 2016 v.170 pp. 220-226
ISSN:
0014-4894
Subject:
Trichinella spiralis; chemotaxis; enzyme-linked immunosorbent assay; in vitro studies; inducible nitric oxide synthase; inflammation; interleukin-1beta; interleukin-8; intestinal mucosa; larvae; messenger RNA; mice; models; molting; monocytes; necrosis; quantitative polymerase chain reaction; secretion; tumor necrosis factor-alpha
Abstract:
... As we all know, invasion of host intestinal epithelium is very important for T. spiralis to complete successfully their life cycle. However, the mechanisms that the intestinal infective larvae (IIL) invade and migrate in the intestinal epithailial cells (IECs) remain unclear until now. The related researches have been hindered since a readily operable in vitro normal model. In our earlier study, a ...
DOI:
10.1016/j.exppara.2016.10.003