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- Ali, Moazzam; Field, Mark C.
- Experimental parasitology 2013 v.134 no.2 pp. 249-255
- Trypanosoma brucei; Western blotting; blood flow; cell biology; cell culture; fluorescence microscopy; gene expression; mutants; parasitology; polymerase chain reaction; protein synthesis; proteins
- ... Ectopic expression of either wild type or mutant proteins is a standard method in cell biology, and a vital part of the tool kit of molecular parasitology. During study of protein expression levels mediating intracellular trafficking, we became aware of highly variable expression between experiments. When investigated systematically it became apparent that ectopic expression of proteins from a rib ...
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