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- Roberts, Sigrid C.; Kline, Chelsey; Liu, Wei; Ullman, Buddy
- Experimental parasitology 2011 v.128 no.2 pp. 166-169
- Leishmania donovani; alleles; chimerism; clones; drugs; gene deletion; gene expression; homologous recombination; inventories; loci; mutants; nucleotide sequences; ornithine; ornithine decarboxylase; parasites; parasitology; phenotype; plasmids; polyamines; transgenes
- ... Leishmania null mutants created by targeted gene replacement are typically complemented with chimeric episomes harboring the replaced gene in order to validate that the observed phenotype is due to the specific gene deletion. However, the current inventory of available episomes for complementation of genetic lesions in Leishmania is unstable in the absence of drug selection, and levels of gene exp ...
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