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1. Function of conserved aromatic residues in the Gal/GalNAc-glycosyltransferase motif of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1

Author:
Tenno, Mari; Saeki, Aki; Elhammer, Åke P.; Kurosaka, Akira
Source:
FEBS journal 2007 v.274 no.23 pp. 6037-6045
ISSN:
1742-464X
Subject:
amino acid substitution; biosynthesis; catalytic activity; enzyme activity; isozymes; leucine; mutants; peptides; transferases; tryptophan
Abstract:
... UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc transferases), which initiate mucin-type O-glycan biosynthesis, have broad acceptor substrate specificities, and it is still unclear how they recognize peptides with different sequences. To increase our understanding of the catalytic mechanism of GalNAc-T1, one of the most ubiquitous isozymes, we studied the effect of substituting s ...
DOI:
10.1111/j.1742-4658.2007.06124.x

2. Tertiary structure in 7.9 m guanidinium chloride - the role of Glu53 and Asp287 in Pyrococcus furiosus endo-β-1,3-glucanase

Author:
Chiaraluce, Roberta; Florio, Rita; Angelaccio, Sebastiana; Gianese, Giulio; van Lieshout, Johan F.T.; van der Oost, John; Consalvi, Valerio
Source:
FEBS journal 2007 v.274 no.23 pp. 6167-6179
ISSN:
1742-464X
Subject:
Pyrococcus furiosus; alanine; binding sites; calcium; carboxylic acids; chelating agents; energy; environmental factors; guanidinium; mutants; mutation; prediction; proteins; surface area; thermodynamics; titration
Abstract:
... The thermodynamic stability of family 16 endo-β-1,3-glucanase (EC 3.2.1.39) from the hyperthermophilic archaeon Pyrococcus furiosus is decreased upon single (D287A, E53A) and double (E53A/D287A) mutation of Asp287 and Glu53. In accordance with the homology model prediction, both carboxylic acids are involved in the composition of a calcium binding site, as shown by titration of the wild-type and t ...
DOI:
10.1111/j.1742-4658.2007.06137.x

3. The Drosophila jumonji gene encodes a JmjC-containing nuclear protein that is required for metamorphosis

Author:
Sasai, Nobuhiro; Kato, Yasuko; Kimura, Gaku; Takeuchi, Takashi; Yamaguchi, Masamitsu
Source:
FEBS journal 2007 v.274 no.23 pp. 6139-6151
ISSN:
1742-464X
Subject:
DNA-directed RNA polymerase; Drosophila; antibodies; cell proliferation; ecdysone; gene expression; genes; heterochromatin; larvae; metamorphosis; mice; mutants; mutation; phenotype; polytene chromosomes; repressor proteins; salivary glands; tissues; transcription (genetics); transgenic animals
Abstract:
... Jumonji (Jmj) is a transcriptional repressor that plays important roles in the suppression of cell proliferation and development of various tissues in the mouse. To further clarify the roles of Jmj during development and gain insight into mechanisms of Jmj-mediated transcriptional regulation, we have taken advantage of Drosophila as a model organism. Drosophila Jmj (dJmj) shares high homology with ...
DOI:
10.1111/j.1742-4658.2007.06135.x

4. The phosphatidylethanolamine level of yeast mitochondria is affected by the mitochondrial components Oxa1p and Yme1p

Author:
Nebauer, Ruth; Schuiki, Irmgard; Kulterer, Birgit; Trajanoski, Zlatko; Daum, Günther
Source:
FEBS journal 2007 v.274 no.23 pp. 6180-6190
ISSN:
1742-464X
Subject:
decarboxylation; homeostasis; membrane proteins; mitochondria; mitochondrial membrane; mutants; phosphatidylethanolamines; phosphatidylserines; protein transport; proteinases; proteolysis; yeasts
Abstract:
... The majority of phosphatidylethanolamine, an essential component of yeast mitochondria, is synthesized by phosphatidylserine decarboxylase 1 (Psd1p), a component of the inner mitochondrial membrane. Here, we report that deletion of OXA1 encoding an inner mitochondrial membrane protein translocase markedly affects the mitochondrial phosphatidylethanolamine level. In an oxa1Δ mutant, cellular and mi ...
DOI:
10.1111/j.1742-4658.2007.06138.x