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- Myers, Cullen L.; Kuiper, Emily G.; Grant, Pei C.; Hernandez, Jennifer; Conn, Graeme L.; Honek, John F.
- FEBS letters 2015 v.589 no.21 pp. 3263-3270
- S-adenosylmethionine; active sites; antibiotic resistance; arginine; asparagine; binding sites; catalytic activity; methyltransferases; mutants; thermodynamics; thiostrepton
- ... Resistance to the antibiotic thiostrepton, in producing Streptomycetes, is conferred by the S-adenosyl-l-methionine (SAM)-dependent SPOUT methyltransferase Tsr. For this and related enzymes, the roles of active site amino acids have been inadequately described. Herein, we have probed SAM interactions in the Tsr active site by investigating the catalytic activity and the thermodynamics of SAM bindi ...
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