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You searched for: Journal FEMS microbiology letters Remove constraint Journal: FEMS microbiology letters Publication Year 2007 Remove constraint Publication Year: 2007 Source 2007 v.270 no.2 Remove constraint Source: 2007 v.270 no.2
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1. Amino acid and nucleotide sequence, adjacent genes, and heterologous expression of hiracin JM79, a sec-dependent bacteriocin produced by Enterococcus hirae DCH5, isolated from Mallard ducks (Anas platyrhynchos)

Author:
Sánchez, Jorge; Diep, Dzung B.; Herranz, Carmen; Nes, Ingolf F.; Cintas, Luis M.; Hernández, Pablo E.
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 227-236
ISSN:
0378-1097
Subject:
Anas platyrhynchos; DNA fragmentation; Enterococcus hirae; amino acids; bacteriocins; ducks; genes; genetically modified organisms; heterologous gene expression; immunity; open reading frames; sequence analysis; signal peptide
Abstract:
... The primary structure of a bacteriocin produced by Enterococcus hirae DCH5 was determined by combined amino acid and DNA sequencing. Nucleotide analysis of a 2838-bp DNA fragment of E. hirae DCH5 revealed five putative ORFs. The first orf (hirJM79) encodes a 74-amino-acid peptide containing an N-terminal signal peptide of 30 amino acids, followed by the amino acid sequence of the mature bacterioci ...
DOI:
10.1111/j.1574-6968.2007.00673.x
PubMed:
17326750

2. ampC gene expression in promoter mutants of cefoxitin-resistant Escherichia coli clinical isolates

Author:
Tracz, Dobryan M.; Boyd, David A.; Hizon, Romeo; Bryce, Elizabeth; McGeer, Allison; Ofner-Agostini, Marianna; Simor, Andrew E.; Paton, Shirley; Mulvey, Michael R.
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 265-271
ISSN:
0378-1097
Subject:
Escherichia coli; cefoxitin; gene overexpression; genes; minimum inhibitory concentration; mutants; mutation; promoter regions; reverse transcriptase polymerase chain reaction; transposons
Abstract:
... Reverse transcriptase polymerase chain reaction was used to determine the amount of overexpression of the ampC gene in 52 cefoxitin-resistant Escherichia coli clinical isolates that had previously characterized mutations in their ampC promoter/attenuator regions. The results showed that mutations that create a consensus -35 box (TTGACA) are the most important factor in strengthening the ampC promo ...
DOI:
10.1111/j.1574-6968.2007.00672.x
PubMed:
17326753

3. An intragenic deletion in pilQ leads to nonpiliation of a Pseudomonas aeruginosa strain isolated from cystic fibrosis lung

Author:
Chang, Yu-Sing Tammy; Klockgether, Jens; Tümmler, Burkhard
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 201-206
ISSN:
0378-1097
Subject:
Pseudomonas aeruginosa; cystic fibrosis; frameshift mutation; habitats; lungs; patients; phenotype; sequence alignment
Abstract:
... Deficient motility is one of the characteristic hallmarks observed in Pseudomonas aeruginosa strains that chronically colonize the lungs of cystic fibrosis (CF) patients. Pseudomonas aeruginosa TB is a nonpiliated CF isolate known to be defective in twitching motility. Complementation confirmed a direct link of this phenotype to an intragenic out-of-frame deletion in pilQ (PA5040). Sequence alignm ...
DOI:
10.1111/j.1574-6968.2007.00664.x
PubMed:
17302936

4. Detecting low concentrations of Shigella sonnei in environmental water samples by PCR

Author:
Hsu, Wen-Bin; Wang, Jiann-Haur; Chen, Pei-Chun; Lu, Yu-Shiou; Chen, Jiann-Hwa
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 291-298
ISSN:
0378-1097
Subject:
Shigella dysenteriae; Shigella sonnei; detection limit; enteroinvasive Escherichia coli; filtration; polymerase chain reaction; water pollution
Abstract:
... Outbreaks of Shigella sonnei associated with contaminated water have been reported and methods for the simultaneous detection of Shigellae and enteroinvasive Escherichia coli in water samples have been developed with detection limits of 10¹-10² CFU mL⁻¹ of water. Because 10¹-10²Shigellae can cause disease, a more sensitive detection method as an addition to the existing methods for detection of Sh ...
DOI:
10.1111/j.1574-6968.2007.00686.x
PubMed:
17391373

5. Efficient downregulation of alb1 gene using an AMA1-based episomal expression of RNAi construct in Aspergillus fumigatus

Author:
Khalaj, Vahid; Eslami, Hamid; Azizi, Mohammad; Rovira-Graells, Nuria; Bromley, Mike
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 250-254
ISSN:
0378-1097
Subject:
Aspergillus fumigatus; RNA interference; fungi; genes; humans; pathogens; phenotype; promoter regions; protoplasts; spores
Abstract:
... An episomal RNAi silencing construct containing the inducible cbhB promoter and a hairpin structure has been made to downregulate the alb1 gene in the human pathogen Aspergillus fumigatus. Transformation of fungal protoplasts resulted in a high number of transformants with an inducible silenced phenotype (white spores). Efficient downregulation of the alb1 gene using this system suggests that this ...
DOI:
10.1111/j.1574-6968.2007.00680.x
PubMed:
17343676

6. Efficient silencing of the gene coding for the ε subunit of DNA polymerase III in Escherichia coli is triggered by antisense RNAs featuring stability in vivo

Author:
Stefan, Alessandra; Tabler, Martin; Hochkoeppler, Alejandro
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 277-283
ISSN:
0378-1097
Subject:
DNA-directed DNA polymerase; Escherichia coli; gene silencing; genes; messenger RNA
Abstract:
... The Escherichia coli gene dnaQ coding for the ε subunit of DNA polymerase III was suppressed in vivo via antisense RNAs. To this aim, different fragments of dnaQ were cloned in reverse orientation into the pBAD-HisB vector or into pT3T7, and the corresponding antisense RNAs were conditionally expressed in vivo. Antisense transcripts featuring fast hybridization in vitro with dnaQ mRNA but lacking ...
DOI:
10.1111/j.1574-6968.2007.00679.x

7. Enhancement of fludioxonil fungicidal activity by disrupting cellular glutathione homeostasis with 2,5-dihydroxybenzoic acid

Author:
Kim, Jong H.; Campbell, Bruce C.; Mahoney, Noreen; Chan, Kathleen L.; Molyneux, Russell J.; May, Gregory S.
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 284
ISSN:
0378-1097
Subject:
pyrroles; antifungal agents; fungicides; synergism; benzoic acid; mitogen-activated protein kinase; signal transduction; glutathione; homeostasis; antioxidant activity; oxidative stress; Aspergillus fumigatus; animal pathogenic fungi; plant pathogenic fungi; Aspergillus flavus; Aspergillus parasiticus; Aspergillus niger; Aspergillus oryzae; Aspergillus nidulellus
Abstract:
... The activity of fludioxonil, a phenylpyrrole fungicide, is elevated by coapplication of the aspirin/salicylic acid metabolite, 2,5-dihydroxybenzoic acid (2,5-DHBA). Fludioxonil activity is potentiated through a mitogen-activated protein kinase (MAPK) pathway that regulates osmotic/oxidative stress-responses. 2,5-DHBA disrupts cellular GSH (reduced glutathione)/GSSG (oxidized glutathione) homeostas ...
Handle:
10113/16471
DOI:
10.1111/j.1574-6968.2007.00682.x
PubMed:
17355596

8. ica and beyond: biofilm mechanisms and regulation in Staphylococcus epidermidis and Staphylococcus aureus

Author:
O'Gara, James P.
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 179-188
ISSN:
0378-1097
Subject:
Staphylococcus aureus; Staphylococcus epidermidis; adhesins; biofilm; biosynthesis; cell walls; epitopes; extracellular matrix; gametolysin; loci; pathogenesis; polysaccharides; protein synthesis; surface proteins; teichoic acids
Abstract:
... Recent progress in elucidating the role of the icaADBC-encoded polysaccharide intercellular adhesin (PIA) or polymeric N-acetyl-glucosamine (PNAG) in staphylococcal biofilm development has in turn contributed significantly to our understanding of the pathogenesis of device-related infections. Nevertheless, our understanding of how the ica locus and PIA/PNAG biosynthesis are regulated is far from c ...
DOI:
10.1111/j.1574-6968.2007.00688.x
PubMed:
17419768

9. Mechanisms of action of ostrich β-defensins against Escherichia coli

Author:
Sugiarto, Haryadi; Yu, Pak-Lam
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 195-200
ISSN:
0378-1097
Subject:
DNA; Escherichia coli; Gram-negative bacteria; RNA; anti-infective properties; antimicrobial peptides; cell membranes; enzyme activity; humans; lipopolysaccharides; mechanism of action; microbial growth; neutrophils; ostriches; protein synthesis; sheep
Abstract:
... To understand their mechanism of antimicrobial activity against Gram-negative bacteria, ostrich β-defensins, ostricacins-1 and 2 (Osp-1 and Osp-2), were compared with those of sheep myeloid antimicrobial peptide (SMAP)-29 and human neutrophil peptide (HNP)-1, well-characterized sheep α-helical and human α-defensin peptides, respectively. Fluorescence-based biochemical assays demonstrated that the ...
DOI:
10.1111/j.1574-6968.2007.00642.x

10. MmcBC in Pelotomaculum thermopropionicum represents a novel group of prokaryotic fumarases

Author:
Shimoyama, Takefumi; Rajashekhara, Eranna; Ohmori, Daijiro; Kosaka, Tomoyuki; Watanabe, Kazuya
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 207-213
ISSN:
0378-1097
Subject:
Escherichia coli; amino acid sequences; electron paramagnetic resonance spectroscopy; molecular weight; temperature
Abstract:
... The overall amino-acid sequence of MmcBC in Pelotomaculum thermopropionicum was substantially homologous (33%) to fumarase A in Escherichia coli, although its possible subunit structure was different from known fumarases and it lacked the fumarate-lyase signature sequence. Here, MmcBC in E. coli is expressed and characterized. The purified enzyme catalyzed reversible conversion of fumarate to l-ma ...
DOI:
10.1111/j.1574-6968.2007.00665.x

11. Oxidative metabolic profiles of Brucella strains isolated from marine mammals: contribution to their species classification

Author:
Jacques, Isabelle; Grayon, Maggy; Verger, Jean-Michel
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 245-249
ISSN:
0378-1097
Subject:
Brucella; Cetacea; aerobiosis; host preferences; marine mammals
Abstract:
... Since the 1990s, Brucella strains not matching the characteristics of any of the six conventional species have been isolated worldwide from marine mammals. In this study, 31 Brucella strains isolated from various marine mammals were examined for their oxidative metabolic pattern on 12 amino-acid and carbohydrate substrates. Three main oxidative profiles different from those of the Brucella terrest ...
DOI:
10.1111/j.1574-6968.2007.00675.x
PubMed:
17326752

12. Polynucleotide phosphorylase independently controls virulence factor expression levels and export in Yersinia spp

Author:
Rosenzweig, Jason A.; Chromy, Brett; Echeverry, Andrea; Yang, Jing; Adkins, Becky; Plano, Gregory V.; McCutchen-Maloney, Sandra; Schesser, Kurt
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 255-264
ISSN:
0378-1097
Subject:
RNA; Yersinia; cell culture; mice; phosphorylase; type III secretion system; virulence
Abstract:
... Previously, it was shown that optimal functioning of the Yersinia type III secretion system (T3SS) in cell culture infection assays requires the exoribonuclease polynucleotide phosphorylase (PNPase) and that normal T3SS activity could be restored in the Δpnp strains by expressing just the ~70-aa S1 RNA-binding domain of PNPase. Here, it is shown that the YersiniaΔpnp strain is less virulent in the ...
DOI:
10.1111/j.1574-6968.2007.00689.x
PubMed:
17391372

13. Quorum-sensing signaling is required for production of the antibiotic pyrrolnitrin in a rhizospheric biocontrol strain of Serratia plymuthica

Author:
Liu, Xiaoguang; Bimerew, Mohammed; Ma, Yingxin; Müller, Henry; Ovadis, Marianna; Eberl, Leo; Berg, Gabriele; Chernin, Leonid
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 299-305
ISSN:
0378-1097
Subject:
Brassica napus; Gram-negative bacteria; Serratia plymuthica; Verticillium wilt; antibiotics; biological control; crops; culture media; genes; mutants; phenazines; plant pathogenic fungi; plasmids; quorum sensing; rhizosphere
Abstract:
... One mechanism that bacteria have adopted to regulate the production of antimicrobial compounds is population-density-dependent LuxRI-type quorum sensing (QS), exploiting the production of N-acyl homoserine lactone (AHL) autoinducer signals. In biocontrol bacteria, most known cases involve the AHL control of phenazine antibiotics production by rhizospheric pseudomonads. This work is the first to de ...
DOI:
10.1111/j.1574-6968.2007.00681.x
PubMed:
17355597

14. Rice endophyte Pantoea agglomerans YS19 forms multicellular symplasmata via cell aggregation

Author:
Duan, Jinyan; Yi, Ting; Lu, Zhenyue; Shen, Delong; Feng, Yongjun
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 220-226
ISSN:
0378-1097
Subject:
Pantoea agglomerans; bacteria; endophytes; green fluorescent protein; mixing; mutation; rice
Abstract:
... Pantoea agglomerans is characterized by the formation of multicellular symplasmata. One unanswered question regarding this bacterium is how these structures are formed. In this study, the rice diazotrophic endophyte P. agglomerans YS19 was selected for exploration of this theme. YS19 was labeled with green fluorescent protein and the resulting recombinant YS19::gfp was observed to grow only slight ...
DOI:
10.1111/j.1574-6968.2007.00677.x
PubMed:
17391364

15. Sequential hydrophobic partitioning of cells of Pseudomonas aeruginosa gives rise to variants of increasing cell-surface hydrophobicity

Author:
Obuekwe, Christian O.; Al-Jadi, Zamya K.; Al-Saleh, Esmaeil S.
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 214-219
ISSN:
0378-1097
Subject:
Pseudomonas aeruginosa; bacteria; bacterial adhesion; electron microscopy; fimbriae; hydrophilicity; hydrophobic interaction chromatography; hydrophobicity; polysaccharides; polystyrenes
Abstract:
... The partitioning of bacterial cells in a dual aqueous-solvent phase system leads to separation into 'hydrophilic' and hydrophobic functions. Sequential multistep partitioning, accompanied by successive enrichment, gives rise to several cycles of hydrophobic and hydrophilic cell populations which possess different cell-surface hydrophobicity characteristics. Characterization of the cell-surface hyd ...
DOI:
10.1111/j.1574-6968.2007.00685.x

16. Silver (Ag⁺) reduces denitrification and induces enrichment of novel nirK genotypes in soil

Author:
Throbäck, Ingela Noredal; Johansson, Mats; Rosenquist, Magnus; Pell, Mikael; Hansson, Mikael; Hallin, Sara
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 189-194
ISSN:
0378-1097
Subject:
community structure; denitrification; denitrifying microorganisms; dose response; genotype; heavy metals; ions; microbial growth; models; nitrite reductase; sewage; silver; soil; soil ecology
Abstract:
... The use of silver ions in industry to prevent microbial growth is increasing and silver is a new and an overlooked heavy-metal contaminant in sewage sludge-amended soil. The denitrifying community was the model used to assess the dose-dependent effects of silver ions on microorganisms overtime in soil microcosms. Silver caused a sigmoid dose-dependent reduction in denitrification activity, and no ...
DOI:
10.1111/j.1574-6968.2007.00632.x

17. The contribution of induction of temperate phages to the numbers of free somatic coliphages in waters is not significant

Author:
Muniesa, Maite; Jofre, Juan
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 272-276
ISSN:
0378-1097
Subject:
bacteria; ciprofloxacin; coliphages; mitomycin; ultraviolet radiation; water quality
Abstract:
... Somatic coliphages have been proposed as indicators of water quality. But several factors have been considered a drawback for their use as indicators. We evaluated the contribution of temperate phages to the numbers of somatic coliphages detected in water by ISO (International Standards Organization) standardised methods. Prophage induction from naturally occurring bacteria was assayed with mitomy ...
DOI:
10.1111/j.1574-6968.2007.00676.x
PubMed:
17343678

18. Variability in occurrence of multiple prophage genes in Salmonella Typhimurium strains isolated in Slovak Republic

Author:
Drahovská, Hana; Mikasová, Eva; Szemes, Tomáš; Ficek, Andrej; Sásik, Milan; Majtán, Viktor; Turna, Ján
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 237-244
ISSN:
0378-1097
Subject:
Salmonella Typhimurium; bacteriophages; genes; minisatellite repeats; polymerase chain reaction; screening; Slovakia
Abstract:
... Lysogenic bacteriophages are a significant source of variability in closely related Salmonella strains. In this study, screening for diversity of 152 Salmonella Typhimurium strains was performed using PCR detection of selected prophage regions derived from phages P22, Gifsy-1, Gifsy-2, Fels-1, ST104 and SopEΦ. A high degree of variability was observed in the presence of specific genes. Based on th ...
DOI:
10.1111/j.1574-6968.2007.00674.x
PubMed:
17355601