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- Khalaj, Vahid; Eslami, Hamid; Azizi, Mohammad; Rovira-Graells, Nuria; Bromley, Mike
- FEMS microbiology letters 2007 v.270 no.2 pp. 250-254
- Aspergillus fumigatus; RNA interference; fungi; genes; humans; pathogens; phenotype; promoter regions; protoplasts; spores
- ... An episomal RNAi silencing construct containing the inducible cbhB promoter and a hairpin structure has been made to downregulate the alb1 gene in the human pathogen Aspergillus fumigatus. Transformation of fungal protoplasts resulted in a high number of transformants with an inducible silenced phenotype (white spores). Efficient downregulation of the alb1 gene using this system suggests that this ...