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You searched for: Journal FEMS microbiology letters Remove constraint Journal: FEMS microbiology letters Publication Year 2007 Remove constraint Publication Year: 2007 Source 2007 v.275 no.2 Remove constraint Source: 2007 v.275 no.2
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1. A longitudinal study to assess the persistence of vancomycin-resistant Enterococcus faecium (VREF) on an intensive broiler farm in the United Kingdom

Author:
Garcia-Migura, Lourdes; Liebana, Ernesto; Jensen, Lars Bogø; Barnes, Simon; Pleydell, Eve
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 319-325
ISSN:
0378-1097
Subject:
Enterococcus faecium; avoparcin; broiler chickens; clones; disinfection; farms; flocks; genetic variation; livestock and meat industry; longitudinal studies; multiple drug resistance; poultry housing; pulsed-field gel electrophoresis; vancomycin; United Kingdom
Abstract:
... Seven years after the ban of avoparcin, VREF could still be isolated within sectors of the UK broiler industry. The aim of this study was to assess whether there is a carryover of VREF between consecutive flocks of birds, to conduct a preliminary investigation of possible routes of entry of VREF into broiler houses and to follow the dynamics of VREF shed by growing birds. A series of nine visits w ...
DOI:
10.1111/j.1574-6968.2007.00911.x
PubMed:
17825067

2. An rmlA gene encoding d-glucose-1-phosphate thymidylyltransferase is essential for mycobacterial growth

Author:
Qu, Hong; Xin, Yi; Dong, Xu; Ma, Yufang
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 237-243
ISSN:
0378-1097
Subject:
Mycobacterium smegmatis; arabinogalactans; bacteria; biosynthesis; cell biology; drugs; enzymes; genes; homologous recombination; mutants; peptidoglycans
Abstract:
... The rhamnose-GlcNAc disaccharide is a critical linker which connects arabinogalactan to peptidoglycan via a phosphodiester linkage. The biosynthesis of dTDP-rhamnose is catalysed by four enzymes, and the first reaction is catalysed by an rmlA gene encoding d-glucose-1-phosphate thymidylyltransferase (RmlA). We generated a Mycobacterium smegmatis mc²155 mutant lacking the rmlA gene via a homologous ...
DOI:
10.1111/j.1574-6968.2007.00890.x
PubMed:
17784859

3. Apoptosis inhibition of alveolar macrophages upon interaction with conidia of Aspergillus fumigatus

Author:
Volling, Katrin; Brakhage, Axel A.; Saluz, Hans Peter
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 250-254
ISSN:
0378-1097
Subject:
Aspergillus fumigatus; animal pathogenic fungi; apoptosis; aspergillosis; caspase-3; conidia; humans; immune response; innate immunity; macrophages
Abstract:
... The opportunistic human pathogenic fungus Aspergillus fumigatus (Af) causes the majority of cases of invasive aspergillosis. Because Af enters the human body through inhalation of airborne conidia, the interaction of conidia with the innate immune system (alveolar macrophages) plays a key role in the etiology of aspergillosis. Therefore, it is of central interest to investigate response mechanisms ...
DOI:
10.1111/j.1574-6968.2007.00883.x
PubMed:
17714483

4. Coprinellus curtus (Hitoyo-take) prevents diseases of vegetables caused by pathogenic fungi

Author:
Nakasaki, Kiyohiko; Saito, Miyuki; Suzuki, Nobuaki
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 286-291
ISSN:
0378-1097
Subject:
Brassica rapa subsp. oleifera; Chinese cabbage; Coprinellus; Fusarium solani; Fusarium wilt; Thanatephorus cucumeris; biological control; disease control; fungi; grasses; hyphae; lettuce; melons; pathogens; root rot; tomatoes
Abstract:
... A strain of Coprinellus curtus (designated GM-21), a basidiomycete that suppressed bottom-rot disease of Chinese cabbage, 'pak-choi' (Brassica campestris), caused by the pathogen Rhizoctonia solani Pak-choi 2 was isolated. The mechanism of plant disease suppression was discovered to be hyphal interference, a combative fungal interaction between strain GM-21 and the pathogen. The antifungal spectru ...
DOI:
10.1111/j.1574-6968.2007.00899.x
PubMed:
17850327

5. Deletion of peb4 gene impairs cell adhesion and biofilm formation in Campylobacter jejuni

Author:
Asakura, Hiroshi; Yamasaki, Manabu; Yamamoto, Shigeki; Igimi, Shizunobu
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 278-285
ISSN:
0378-1097
Subject:
Campylobacter jejuni; adhesion; bacteria; biofilm; cell adhesion; genes; intestines; mice; mutants; pathogens; proteins; proteomics
Abstract:
... Campylobacter jejuni is a microaerophilic bacterium that causes diarrhea in humans. The first step in establishing an infection is adherence to a host cell, which involves two major cell-binding proteins, Peb1A (CBF1) and Peb4 (CBF2). Because the functional role of Peb4 on the cell adhesion remains unclear compared with that of Peb1A, a C. jejuni peb4 deletion mutant was constructed and cell adher ...
DOI:
10.1111/j.1574-6968.2007.00893.x
PubMed:
17714477

6. Effects of three benzimidazoles on growth, general morphology and ultrastructure of Tritrichomonas foetus

Author:
Carvalho, Karina Penedo; Gadelha, Ana Paula Rocha
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 292-300
ISSN:
0378-1097
Subject:
Tritrichomonas foetus; albendazole; cattle; embryonic mortality; flagellum; mebendazole; organelles; parasites; pathogens; therapeutics; thiabendazole; trichomoniasis; ultrastructure
Abstract:
... Tritrichomonas foetus is a venereal pathogen of cattle, which causes infertility, early embryonic death or abortion. In order to evaluate the potential trichomonicidal activity of benzimidazoles, the effects of thiabendazole, mebendazole and albendazole were analyzed on the multiplication, general morphology and ultrastructure of T. foetus. It was found that mebendazole presented the highest IC₅₀% ...
DOI:
10.1111/j.1574-6968.2007.00897.x
PubMed:
17825070

7. Expression analysis of the colibactin gene cluster coding for a novel polyketide in Escherichia coli

Author:
Homburg, Stefan; Oswald, Eric; Hacker, Jörg; Dobrindt, Ulrich
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 255-262
ISSN:
0378-1097
Subject:
Escherichia coli; RNA-directed DNA polymerase; bacteria; carbon; coculture; cytopathogenicity; gene expression; luciferase; multigene family; operon; reporter genes; reverse transcriptase polymerase chain reaction; transcription (genetics)
Abstract:
... The recently described hybrid nonribosomal peptide-polyketide colibactin, found in various Escherichia coli strains, invokes a cytopathic effect in HeLa cells upon cocultivation with these bacteria. However, not much is known so far about the transcriptional organization of the colibactin genes (clb) or the regulation of their transcription. Here, the operon structure of the colibactin gene cluste ...
DOI:
10.1111/j.1574-6968.2007.00889.x
PubMed:
17714479

8. Identification of a new hemolysin from diarrheal isolate SSU of Aeromonas hydrophila

Author:
Erova, Tatiana E.; Sha, Jian; Horneman, Amy J.; Borchardt, Mark A.; Khajanchi, Bijay K.; Fadl, Amin A.; Chopra, Ashok K.
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 301-311
ISSN:
0378-1097
Subject:
Aeromonas hydrophila; DNA fingerprinting; Escherichia coli; Shewanella oneidensis; Vibrio vulnificus; antibodies; cystathionine; cytotoxicity; diarrhea; enterotoxins; gene overexpression; genes; hemolysins; hemolysis; molecular weight; mutants; nucleotide sequences; trypsin; virulence
Abstract:
... A clinical strain SSU of Aeromonas hydrophila produces a potent cytotoxic enterotoxin (Act) with cytotoxic, enterotoxic, and hemolytic activities. A new gene, which encoded a hemolysin of 439-amino acid residues with a molecular mass of 49 kDa, was identified. This hemolysin (HlyA) was detected based on the observation that the act gene minus mutant of A. hydrophila SSU still had residual hemolyti ...
DOI:
10.1111/j.1574-6968.2007.00895.x
PubMed:
17725618

9. Identification of genes differentially expressed in the phytopathogenic fungus Cercospora nicotianae between cercosporin toxin-resistant and -susceptible strains

Author:
Herrero, Sonia; Amnuaykanjanasin, Alongkorn; Daub, Margaret E.
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 326-337
ISSN:
0378-1097
Subject:
Cercospora nicotianae; H+/K+-exchanging ATPase; adenosinetriphosphatase; antioxidants; biosynthesis; expressed sequence tags; gene expression regulation; genes; mutants; pH; plant pathogenic fungi; reactive oxygen species; suppression subtractive hybridization; toxicity; transcription factors; transporters
Abstract:
... Plant pathogens from the genus Cercospora produce cercosporin, a photoactivated fungal toxin that generates toxic reactive oxygen species. Mechanisms governing toxin auto-resistance in Cercospora spp. are poorly understood. In this work, suppressive subtractive hybridization was used to identify genes differentially expressed between the cercosporin-resistant wild-type (WT) Cercospora nicotianae a ...
DOI:
10.1111/j.1574-6968.2007.00903.x
PubMed:
17850326

10. Identification of Tannerella forsythia antigens specifically expressed in patients with periodontal disease

Author:
Yoo, Ji Yeon; Kim, Hyeong Chan; Zhu, Weidong; Kim, Seon-Mi; Sabet, Mojgan; Handfield, Martin; Hillman, Jeffrey; Progulske-Fox, Ann; Lee, Seok-Woo
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 344-352
ISSN:
0378-1097
Subject:
Bacteroides forsythus; DNA; DNA repair; antigens; base pair mismatch; clones; dipeptidyl-peptidase IV; gene expression; genes; glycine hydroxymethyltransferase; ligases; nucleotide sequences; outer membrane proteins; pathogenesis; pathogens; patients; periodontal diseases; quantitative polymerase chain reaction; transfer RNA; virulence
Abstract:
... Molecular pathogenesis of Tannerella forsythia, a putative periodontal pathogen, has not yet been adequately elucidated due to limited information on its virulence factors. Here, identification of in vivo expressed antigens of T. forsythia is reported using in vivo-induced antigen technology (IVIAT). Among 13 000 recombinant clones screened, 16 positive clones were identified that reacted reproduc ...
DOI:
10.1111/j.1574-6968.2007.00906.x
PubMed:
17868359

11. Impact of the small RNA RyhB on growth, physiology and heterologous protein expression in Escherichia coli

Author:
Bollinger, Christian J.T.; Kallio, Pauli T.
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 221-228
ISSN:
0378-1097
Subject:
Escherichia coli; Vitreoscilla; acetates; electron transport chain; enzymes; gene overexpression; green fluorescent protein; hemoglobin; heterologous gene expression; homeostasis; mutants; non-coding RNA; tricarboxylic acid cycle
Abstract:
... The small noncoding RNA RyhB is a regulator of iron homeostasis in Escherichia coli. During iron limitation, it downregulates the expression of a number of iron-containing proteins, including enzymes of the tricarboxylic acid cycle and the respiratory chain. Because this infers a potential for RyhB to limit energy metabolism and biosynthetic capacity, the effect of knocking out ryhB on the physiol ...
DOI:
10.1111/j.1574-6968.2007.00880.x
PubMed:
17784860

12. In silico comparison of pKLC102-like genomic islands of Pseudomonas aeruginosa

Author:
Würdemann, Dieco; Tümmler, Burkhard
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 244-249
ISSN:
0378-1097
Subject:
DNA replication; Pseudomonas aeruginosa; genes; genomic islands; open reading frames; replication origin
Abstract:
... The genomic island pKLC102 first detected in Pseudomonas aeruginosa clone C strains can cross species barriers and exhibits the highest mobilization rate of a genomic island known to date. Homologous genomic islands of 81-108 kb in size were identified in the completely sequenced P. aeruginosa strains PA7, PA14, 2192, C3719 and PACS2, but not in strains PAO1 and LES. All pKLC102-like genomic islan ...
DOI:
10.1111/j.1574-6968.2007.00891.x
PubMed:
17714478

13. In vivo colonization profile study of Bordetella bronchiseptica in the nasal cavity

Author:
Irie, Yasuhiko; Yuk, Ming H.
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 191-198
ISSN:
0378-1097
Subject:
Bordetella bronchiseptica; adhesins; epithelium; hemagglutinins; mammals; nasal cavity; respiratory tract diseases; virulence
Abstract:
... Bordetella bronchiseptica chronically infects a wide range of mammals, and resides primarily in the nasal cavity of the infected host. Multiple virulence factors of Bordetella species have been studied in the context of lower respiratory tract infections, but relatively less is known about the bacterial life cycle in the nasal cavity. Evidences were discovered for Bvg intermediate (Bvgi) phase exp ...
DOI:
10.1111/j.1574-6968.2007.00852.x
PubMed:
17714485
PubMed Central:
PMC2121150

14. Metal-dependent repression of siderophore and biofilm formation in Actinomyces naeslundii

Author:
Moelling, Cas; Oberschlacke, Ross; Ward, Price; Karijolich, John; Borisova, Ksenia; Bjelos, Nikola; Bergeron, Lori
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 214-220
ISSN:
0378-1097
Subject:
Actinomyces naeslundii; Gallionellaceae; bacteria; biofilm; culture media; fungi; gels; gene expression; genes; iron; models; mouth; promoter regions; siderophores; virulence
Abstract:
... Actinomyces naeslundii is a pioneer of the oral cavity and forms a biofilm on the tooth's surface. Most bacteria require iron for survival and in pathogenic bacteria iron availability regulates virulence gene expression. Metal-dependent repressors control gene expression involved in metal transport and uptake including siderophores. Siderophores are small molecules synthesized by bacteria and fung ...
DOI:
10.1111/j.1574-6968.2007.00888.x

15. Mutation rate is reduced by increased dosage of mutL gene in Escherichia coli K-12

Author:
Galán, Juan-Carlos; Turrientes, María-Carmen; Baquero, María-Rosario; Rodríguez-Alcayna, Manuel; Martínez-Amado, Jorge; Martínez, José-Luis; Baquero, Fernando
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 263-269
ISSN:
0378-1097
Subject:
Escherichia coli K12; genes; mutation; phenotype
Abstract:
... A variable but substantial proportion of wild Escherichia coli isolates present consistently lower mutation frequencies than that found in the ensemble of strains. The genetic mechanisms responsible for the hypo-mutation phenotype are much less known than those involved in hyper-mutation. Changes in E. coli mutation frequencies derived from the gene-copy effect of mutS, mutL, mutH, uvrD, mutT, mut ...
DOI:
10.1111/j.1574-6968.2007.00902.x

16. Phosphatidylcholine synthesis in Crithidia deanei: the influence of the endosymbiont

Author:
de Azevedo-Martins, Allan Cézar; Frossard, Mariana Lins; de Souza, Wanderley; Einicker-Lamas, Marcelo; Motta, Maria Cristina Machado
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 229-236
ISSN:
0378-1097
Subject:
Crithidia; bacteria; biochemical pathways; biosynthesis; cell walls; endosymbionts; mitochondria; phosphatidylcholines; phosphatidylethanolamines; prokaryotic cells; symbiosis
Abstract:
... In this study, the role of phospholipid biosynthetic pathways was investigated in the establishment of the mutualistic relationship between the trypanosomatid protozoan Crithidia deanei and its symbiotic bacterium. Although the endosymbiont displays two unit membranes, it lacks a typical Gram-negative cell wall. As in other intracellular bacteria, phosphatidylcholine is a major component of the sy ...
DOI:
10.1111/j.1574-6968.2007.00892.x
PubMed:
17714482

17. Protective role and regulation of Rad9 from the fission yeast Schizosaccharomyces pombe

Author:
Kang, Min-Hee; Park, Eun-Hee; Lim, Chang-Jin
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 270-277
ISSN:
0378-1097
Subject:
RNA-directed DNA polymerase; Schizosaccharomyces pombe; beta-galactosidase; gene expression; gene fusion; genes; genetic vectors; malnutrition; messenger RNA; nitric oxide; nitrogen; nitroprusside; plasmids; protective effect; starvation; vector control
Abstract:
... To assess novel cellular roles and regulation of Rad9 in the fission yeast Schizosaccharomyces pombe, the full-length rad9 gene was cloned into the shuttle vector pRS316, generating pYFRad9. The rad9 mRNA level was significantly increased in the S. pombe cells harboring the plasmid pYFRad9, suggesting that the cloned rad9 gene is functioning. The S. pombe cells harboring pYFRad9 showed higher surv ...
DOI:
10.1111/j.1574-6968.2007.00898.x
PubMed:
17725619

18. Recent advances in molecular techniques for the detection of phylogenetic markers and functional genes in microbial communities

Author:
Lau, Stanley C.K.; Liu, Wen-Tso
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 183-190
ISSN:
0378-1097
Subject:
functional diversity; genes; microbial communities; microorganisms; nucleic acids; phylogeny; species diversity
Abstract:
... The detection and analysis of nucleic acids extracted from microbial communities are the ultimate ways to determine the diversity and functional capability of microbial communities in the environments. However, it remains a challenge to use molecular techniques for unequivocal determination and quantification of microbial species composition and functional activities. Considerable efforts have bee ...
DOI:
10.1111/j.1574-6968.2007.00853.x

19. Specific mutations in the Mycobacterium tuberculosis rpoB gene are associated with increased dnaE2 expression

Author:
Bergval, Indra L.; Klatser, Paul R.; Schuitema, Anja R.J.; Oskam, Linda; Anthony, Richard M.
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 338-343
ISSN:
0378-1097
Subject:
DNA-directed DNA polymerase; Mycobacterium tuberculosis; bacteria; cell viability; genes; mutants; mutation; probability; quantitative polymerase chain reaction; rifampicin; stress response
Abstract:
... In Mycobacterium tuberculosis (MTB), rifampicin resistance is almost invariably due to mutations in the rpoB gene, whose function is critical for cell viability. Most of these mutations, at least initially, impair the fitness of the bacteria but confer a selective advantage when antibiotic pressure is exerted. Subsequent adaptation may be critical to restore fitness. The possibility was considered ...
DOI:
10.1111/j.1574-6968.2007.00905.x
PubMed:
17868360

20. Use of green fluorescent protein to monitor Lactobacillus in the gastro-intestinal tract of chicken

Author:
Yu, Qing-Hua; Dong, Suo-Meng; Zhu, Wei-Yun; Yang, Qian
Source:
FEMS microbiology letters 2007 v.275 no.2 pp. 207-213
ISSN:
0378-1097
Subject:
Lactobacillus delbrueckii subsp. lactis; Leuconostoc fructosum; bacteria; cecum; chickens; epithelium; fermentation; fluorescence; fluorescence microscopy; genes; green fluorescent protein; ileum; ingestion; jejunum; liver; mucus; oral administration; probiotics; spleen; stomach
Abstract:
... Lactobacilli, like other gut commensal bacteria, are well known for their use in industrial food fermentations and for their probiotic properties. However, little is known about the interaction of these microorganisms with the gastro-intestinal epithelia when administered in vivo. To specifically monitor the passage of lactobacilli after oral administration, the gfp gene was cloned downstream from ...
DOI:
10.1111/j.1574-6968.2007.00877.x
PubMed:
17714484